ROS-related Enzyme Expressions in Endothelial Cells Regulated by Tea Polyphenols

CHEN-JIANG YING; XIU-FA SUN; Shu-Lin Zhang; XI-PING ZHANG; LI-MEI MAO; XUE-ZHI ZUO; PING YAO

PDF
Cite
Share

facebook

twitter

google

linkedin

Volume 17 Issue 1

Mar. 2004

Turn off MathJax

Article Contents

Article Navigation > Biomedical and Environmental Sciences > 2004 > 17(1): 33-39

CHEN-JIANG YING, XIU-FA SUN, Shu-Lin Zhang, XI-PING ZHANG, LI-MEI MAO, XUE-ZHI ZUO, PING YAO. ROS-related Enzyme Expressions in Endothelial Cells Regulated by Tea Polyphenols[J]. Biomedical and Environmental Sciences, 2004, 17(1): 33-39.

Citation:

CHEN-JIANG YING, XIU-FA SUN, Shu-Lin Zhang, XI-PING ZHANG, LI-MEI MAO, XUE-ZHI ZUO, PING YAO. ROS-related Enzyme Expressions in Endothelial Cells Regulated by Tea Polyphenols[J]. Biomedical and Environmental Sciences, 2004, 17(1): 33-39.

ROS-related Enzyme Expressions in Endothelial Cells Regulated by Tea Polyphenols

Department of Nutrition and Food Hygiene,Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, Hubei, China
School of Public Health,Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, Hubei, China
Tongji Hospital,Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, Hubei, China

Funds: This study was supported in part by the Japan-China Sasakawa Medical Fellowship

Abstract

Objective Elevation of reactive oxygen species (ROS), especially the level of superoxide is a key event in many forms of cardiovascular diseases. To study the mechanism of tea polyphenols against cardiovascular diseases, we observed the expressions of ROS-related enzymes in endothelial cells. Methods Tea polyphenols were co-incubated with bovine carotid artery endothelial cells (BCAECs) in vitro and intracellular NADPH oxidase subunits p22phox and p67phox, SOD-1, and catalase protein were detected using Western blot method. Results Tea polyphenols of 0.4 μg/mL and 4.0 μg/mL (from either green tea or black tea) down-regulated NADPH oxidase p22phox and p67phox expressions in a dose-negative manner (P＜0.05), and up-regulated the expressions of catalase (P＜0.05). Conclusions Tea polyphenols regulate the enzymes involved in ROS production and elimination in endothelial cells, and may be beneficial to the prevention of endothelial cell dysfunction and the development of cardiovascular diseases.
- Tea polyphenols,
- Endothelial cells,
- NADPH oxidase,
- Catalase,
- Western blot
References
Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article Metrics

Article views(1330) PDF downloads(42) Cited by()

Proportional views

ROS-related Enzyme Expressions in Endothelial Cells Regulated by Tea Polyphenols

Department of Nutrition and Food Hygiene,Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, Hubei, China

School of Public Health,Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, Hubei, China

Tongji Hospital,Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, Hubei, China

Funds: This study was supported in part by the Japan-China Sasakawa Medical Fellowship

Key words:

Abstract: Objective Elevation of reactive oxygen species (ROS), especially the level of superoxide is a key event in many forms of cardiovascular diseases. To study the mechanism of tea polyphenols against cardiovascular diseases, we observed the expressions of ROS-related enzymes in endothelial cells. Methods Tea polyphenols were co-incubated with bovine carotid artery endothelial cells (BCAECs) in vitro and intracellular NADPH oxidase subunits p22phox and p67phox, SOD-1, and catalase protein were detected using Western blot method. Results Tea polyphenols of 0.4 μg/mL and 4.0 μg/mL (from either green tea or black tea) down-regulated NADPH oxidase p22phox and p67phox expressions in a dose-negative manner (P＜0.05), and up-regulated the expressions of catalase (P＜0.05). Conclusions Tea polyphenols regulate the enzymes involved in ROS production and elimination in endothelial cells, and may be beneficial to the prevention of endothelial cell dysfunction and the development of cardiovascular diseases.

Citation:

CHEN-JIANG YING, XIU-FA SUN, Shu-Lin Zhang, XI-PING ZHANG, LI-MEI MAO, XUE-ZHI ZUO, PING YAO. ROS-related Enzyme Expressions in Endothelial Cells Regulated by Tea Polyphenols[J]. Biomedical and Environmental Sciences, 2004, 17(1): 33-39.