JI Ya Wei; KONG Yan; ZHAO Ya Hong; WANG Ya Ling; ZHAO Jing; ZHANG Lu Zhong; YANG Yu Min

doi:10.3967/bes2014.139

Controlling the Release of bFGF from Silk Fibroin Membrane

JI Ya Wei, KONG Yan, ZHAO Ya Hong, WANG Ya Ling, ZHAO Jing, ZHANG Lu Zhong, YANG Yu Min

文章导航 > Biomedical and Environmental Sciences > 2014 > 27(12): 973-977

JI Ya Wei, KONG Yan, ZHAO Ya Hong, WANG Ya Ling, ZHAO Jing, ZHANG Lu Zhong, YANG Yu Min. Controlling the Release of bFGF from Silk Fibroin Membrane[J]. Biomedical and Environmental Sciences, 2014, 27(12): 973-977. doi: 10.3967/bes2014.139

Citation:

JI Ya Wei, KONG Yan, ZHAO Ya Hong, WANG Ya Ling, ZHAO Jing, ZHANG Lu Zhong, YANG Yu Min. Controlling the Release of bFGF from Silk Fibroin Membrane[J]. Biomedical and Environmental Sciences, 2014, 27(12): 973-977. doi: 10.3967/bes2014.139

doi: 10.3967/bes2014.139

基金项目: the National High Technology Research and Development Program of China(2012AA020502)%National Natural Science Foundation of China(81171457 and 81371687)%the Priority of Academic Program Development of Jiangsu Higher Education Institutions%Natural Science Foundation of Jiangsu Province of China(BK20130390)

Controlling the Release of bFGF from Silk Fibroin Membrane

Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, Jiangsu, China; The Neural Regeneration Co-innovation Center of Jiangsu Province, Nantong University, Nantong 226001, Jiangsu, China
College of Chemistry and Chemical Engineering, Nantong University, Nantong 226001, Jiangsu, China
Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, Jiangsu, China

Funds: the National High Technology Research and Development Program of China(2012AA020502)%National Natural Science Foundation of China(81171457 and 81371687)%the Priority of Academic Program Development of Jiangsu Higher Education Institutions%Natural Science Foundation of Jiangsu Province of China(BK20130390)

摘要: Since neurotrophic factor is easy to degrade and aggregate,it usually has a short half-life in vitro.To overcome this shortage,neurotrophic factor has been combined with the silk fibroin (SF) membrane to realize less degradation,optimal loading efficiency,sustained release,and good adsorption.By optimizing its binding conditions,main parameters were investigated and its optimal loading efficiency was obtained.bFGF was combined to SF membrane by layer by layer (LbL) static adsorption technique.The natural and nontoxic chondroitin sulfate (CS) was used as a crosslinking agent.Optimization was carried out in three aspects:the concentration of bFGF,the concentration of CS,and the reaction time.This experiment provides a better environment for the growth of cells and offers a new kind material of absorbing neurotrophic factor to meet increasing demand for biological materials.
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- neurotrophic factor /
- biological materials /
- crosslinking agent /
- reaction time /
- silk fibroin /
- the growth /
- in vitro

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Controlling the Release of bFGF from Silk Fibroin Membrane

doi: 10.3967/bes2014.139

刊出日期: 2014-12-20

关键词:

neurotrophic factor /

biological materials /

the growth /

摘要: Since neurotrophic factor is easy to degrade and aggregate,it usually has a short half-life in vitro.To overcome this shortage,neurotrophic factor has been combined with the silk fibroin (SF) membrane to realize less degradation,optimal loading efficiency,sustained release,and good adsorption.By optimizing its binding conditions,main parameters were investigated and its optimal loading efficiency was obtained.bFGF was combined to SF membrane by layer by layer (LbL) static adsorption technique.The natural and nontoxic chondroitin sulfate (CS) was used as a crosslinking agent.Optimization was carried out in three aspects:the concentration of bFGF,the concentration of CS,and the reaction time.This experiment provides a better environment for the growth of cells and offers a new kind material of absorbing neurotrophic factor to meet increasing demand for biological materials.