Evaluation of the Sensitivity and Specificity of the New Clinical Diagnostic and Classification Criteria for Kashin-Beck Disease, an Endemic Osteoarthritis, in China

YU Fang Fang PING Zhi Guang YAO Chong WANG Zhi Wen WANG FuQi GUO Xiong

YU Fang Fang, PING Zhi Guang, YAO Chong, WANG Zhi Wen, WANG FuQi, GUO Xiong. Evaluation of the Sensitivity and Specificity of the New Clinical Diagnostic and Classification Criteria for Kashin-Beck Disease, an Endemic Osteoarthritis, in China[J]. Biomedical and Environmental Sciences, 2017, 30(2): 150-155. doi: 10.3967/bes2017.021
Citation: YU Fang Fang, PING Zhi Guang, YAO Chong, WANG Zhi Wen, WANG FuQi, GUO Xiong. Evaluation of the Sensitivity and Specificity of the New Clinical Diagnostic and Classification Criteria for Kashin-Beck Disease, an Endemic Osteoarthritis, in China[J]. Biomedical and Environmental Sciences, 2017, 30(2): 150-155. doi: 10.3967/bes2017.021

doi: 10.3967/bes2017.021
基金项目: 

the National Natural Scientific Foundation of China 81620108026

the National Natural Scientific Foundation of China 81472924

Evaluation of the Sensitivity and Specificity of the New Clinical Diagnostic and Classification Criteria for Kashin-Beck Disease, an Endemic Osteoarthritis, in China

Funds: 

the National Natural Scientific Foundation of China 81620108026

the National Natural Scientific Foundation of China 81472924

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    Author Bio:

    YU Fang Fang, male, born in 1987, PhD, majoring in pathogenesis of Kashin-Beck disease

    PING Zhi Guang, male, born in 1978, PhD, Associate Professor, majoring in medical data management and mining

    Corresponding author: GUO Xiong, Tel:86-29-82655091, Fax:86-29-82655032; E-mail:guox@mail.xjtu.edu.cn
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  • 收稿日期:  2016-05-05
  • 录用日期:  2016-12-25
  • 刊出日期:  2017-02-20

Evaluation of the Sensitivity and Specificity of the New Clinical Diagnostic and Classification Criteria for Kashin-Beck Disease, an Endemic Osteoarthritis, in China

doi: 10.3967/bes2017.021
    基金项目:

    the National Natural Scientific Foundation of China 81620108026

    the National Natural Scientific Foundation of China 81472924

    作者简介:

    YU Fang Fang, male, born in 1987, PhD, majoring in pathogenesis of Kashin-Beck disease

    PING Zhi Guang, male, born in 1978, PhD, Associate Professor, majoring in medical data management and mining

    通讯作者: GUO Xiong, Tel:86-29-82655091, Fax:86-29-82655032; E-mail:guox@mail.xjtu.edu.cn

English Abstract

YU Fang Fang, PING Zhi Guang, YAO Chong, WANG Zhi Wen, WANG FuQi, GUO Xiong. Evaluation of the Sensitivity and Specificity of the New Clinical Diagnostic and Classification Criteria for Kashin-Beck Disease, an Endemic Osteoarthritis, in China[J]. Biomedical and Environmental Sciences, 2017, 30(2): 150-155. doi: 10.3967/bes2017.021
Citation: YU Fang Fang, PING Zhi Guang, YAO Chong, WANG Zhi Wen, WANG FuQi, GUO Xiong. Evaluation of the Sensitivity and Specificity of the New Clinical Diagnostic and Classification Criteria for Kashin-Beck Disease, an Endemic Osteoarthritis, in China[J]. Biomedical and Environmental Sciences, 2017, 30(2): 150-155. doi: 10.3967/bes2017.021
  • Kashin-Beck disease (KBD) is an endemic, chronic, and degenerative form of osteoarthritis, characterized by deformity and disability[1-3]. KBD occurs most commonly in children aged 5-15 years and is characterized by the degeneration and necrosis of epiphyseal plate cartilage as well as articular cartilage at several other locations in the developing skeleton. The clinical syndrome includes joint pain and enlargement, restricted movement, short fingers, and dwarfism. Severe patients experience a loss of capacity for physical labor in adulthood and lifelong disability[4-5]. A previous study has described 20 clinical symptoms and four radiological signs in various joints of 2, 560 KBD patients from endemic and non-endemic areas in China[6]. It has been suggested that the symptoms should be classified into the following five groups, representing the manifestations of KBD: flexion of the distal joint of fingers or deformed fingers, arthritic pain in the knee and ankle joints, enlargement of small-and middle-sized joints, limited motion in affected joints with deformity, and retarded growth[7-10].

    Appropriate diagnostic criteria are important for the surveillance, prevention, control, and elimination of KBD. In 1995, the National Health and Family Planning Commission of the People's Republic of China promulgated and implemented 'Diagnosis Criteria for Kashin-Beck Disease (GB16003-1995)'[11]. These criteria were developed by the Ministry of Health based on KBD research data collected since 1956, diagnostic criteria of KBD research consortium (three northeast provinces) since 1972, diagnostic criteria of the central endemic disease office since 1983, and the Yongshou study group of clinical diagnosis and X-ray diagnostic criteria since 1984[12]. These criteria (GB16003-1995) have been employed for surveillance, therapy, and research for 15 years. In 2010, the National Health and Family Planning Commission of the People's Republic of China promulgated and implemented new 'Diagnostic Criteria for Kashin-Beck Disease (WS/T207-2010)'[13]. This new criteria integrated and revised both 'Diagnostic Criteria for Kashin-Beck Disease (GB16003-1995)'[14] and 'X-ray classification indexing for Kashin-Beck disease (WS/T207-2001)' (Table 1). The new diagnosis criteria (WS/T207-2010) were convenient and simple for epidemiological surveillance and had extensive clinical applications.

    Table 1.  The Two Different Diagnostic Criteria of Kashin-Beck Disease

    Criterion KBD Endemic Areas, Clinical Symptoms, and X-ray Signs
    GB16003-1995 (gold criterion) Early stage Mild limited and pain in hand, wrist, knee and ankle joint, multiple symmetric flexion of distal part of fingers, metaphyseal lesions in phalanges
    Ⅰ stage Multiple symmetric enlarged finger joints, restricted stretch, mild muscle atrophy and clinical symptoms of early stage, metaphyseal or epiphyseal lesions in phalanges
    Ⅱ stage Shortened fingers and clinical symptoms of first stage, early closure of epiphysial plate in phalanges
    Ⅲ stage Dwarfism and clinical symptoms of second stage, aggravated metaphyseal lesions in phalanges
    WS/T207-2010 (new criterion) Ⅰ stage Enlarged finger joints, limited and pain in the joints of the limbs
    Ⅱ stage Shortened fingers and clinical symptoms of first stage
    Ⅲ stage Dwarfism and clinical symptoms of second stage

    This study aimed to evaluate the sensitivity and specificity of the new clinical diagnostic and classification criteria for KBD compared with those of the old criteria (GB16003-1995). We sampled 3, 459 KBD patients in Yongshou County using the new 'Diagnostic Criteria for Kashin-Beck Disease (GB16003-1995)' in 2002. Subsequently, we performed a screening test for six clinical symptoms in this population (n=25, 304) using the new 'Diagnostic Criteria for Kashin-Beck Disease (WS/T207-2010)' to determine its sensitivity and specificity.

  • This study was conducted in 110 villages in Linyou County in 2002. Stratified random sampling was performed according to the prevalence of KBD in the villages. The 110 villages in Linyou County were divided into three groups: 13 low-prevalence villages ( < 10%), 42 medium-prevalence villages (10%-20%), and 55 high-prevalence villages ( > 20%). One-third of the villages in each group were sampled: 36 villages, including four low-prevalence villages, 14 medium-prevalence villages, and 18 high-prevalence villages. In total, 25, 304 individuals were surveyed, and all participants provided written informed consent according to the Declaration of Helsinki. Ethical approval on the research was obtained from the Xi'an Jiaotong University.

  • We surveyed baseline characteristics and clinical diagnosis in accordance with the research objective. Baseline characteristics included name, sex, age, ethnicity, occupation, and age at the incidence. Clinical diagnosis included flexion of the distal part of fingers, deformed fingers, enlarged finger joints, shortened fingers, squat down, and dwarfism (Figure 1). We first identified 3, 459 KBD patients using the old 'Diagnostic Criteria for Kashin-Beck Disease (GB16003-1995)'. Subsequently, we performed a screening test using the new 'Diagnostic Criteria for Kashin-Beck Disease (WS/T207-2010)' (Table 1), and evaluated the sensitivity and specificity of the new criteria.

    Figure 1.  The KBD patients were classified as stages Ⅰ, Ⅱ, or Ⅲ KBD using the new diagnostic criteria for Kashin-Beck disease (WS/T207-2010). Stage Ⅰ KBD is depicted in A (enlarged finger joints) and B (enlarged finger joints and flexion of the distal part of fingers), stage Ⅱ KBD is depicted in C (shortened fingers and enlarged finger joints), and stage Ⅲ KBD is depicted in D (dwarfism).

  • (ⅰ) Shortened fingers: The phalanges of healthy participants and patients with developmental disorder were compared. We found that the axis length of phalanges of patients was much shorter (25%±2.5%) than the axis length of phalanges of healthy participants. (ⅱ) Deformed fingers: Fingers with different directions of skew were termed as 'deformed'. (ⅲ) Enlarged finger joints: The finger joints of patients with bony enlargements and multiple clinical signs were considered as 'enlarged'. (ⅳ) Flexion of the distal part of fingers: Distal flexion occurred more commonly in the Ⅱ, Ⅲ, and Ⅳ fingers. Straight fingers were parallel to the view of inspection, and physical examination was not performed at cold temperatures.

  • Data entry and statistical analyses were performed using the Excel and the SPSS version 16.0 software packages (IBM Corporation, United States). Sensitivity refers to the test's ability to correctly detect KBD patients with clinical symptoms. Mathematically, this can be calculated as follows: Sensitivity=Number of true positives / (Number of true positives + Number of false negatives). Specificity relates to the test's ability to correctly detect KBD patients without clinical symptoms. Mathematically, this can be calculated as follows: Specificity=Number of true negatives / (Number of true negatives + Number of false positives)[15]. The Youden index represents the total ability of the screening test to identify true patients and non-patients. It can be calculated using the following formula: The Youden index=Sensitivity + Specificity-1. A larger Youden index value indicates higher diagnostic accuracy[16].

  • We conducted a sampling survey of one-third of the total population in Linyou County, where the investigated 25, 304 Han Chinese patients were belonged to farming families. We identified 3, 459 KBD patients [1, 953 males (56.5%) and 1, 506 females (43.5%); average age, 46±14 years] based on the history and clinical examination using the old 'Diagnostic Criteria of Kashin-Beck Disease (GB16003-1995)'. The 3, 459 KBD patients were classified into four stages: early stage (69 patients), stage Ⅰ (1, 952 patients), stage Ⅱ (1, 132 patients), and stage Ⅲ (306 patients). The population of 21, 845 healthy people included 11, 305 males (51.8%) and 10, 540 females (48.2%) with average age of 39±18 years (Table 2). The age distribution revealed that the peak prevalence of KBD was at 40-49 years of age, with more than 25% prevalence rate, where as KBD patients of 0-19 years had lower prevalence rate ( < 5%) (Figure 2). The majority of patients had stage Ⅰ KBD (56.4%), followed by stage Ⅱ KBD (32.7%), and stage Ⅲ (8.8%). The ratio of stages Ⅰ, Ⅱ, and Ⅲ KBD was approximately 6:3:1.

    Table 2.  The Baseline Characteristics of a Screening Population in Linyou Contry

    Groups Kashin-Beck Disease Healthy
    Early Stage Ⅰ Stage Ⅱ Stage Ⅲ Stage Total
    Sex (n) Male/female 49/20 1, 066/886 670/462 168/138 1, 953/1, 506 11, 305/10, 540
    Occupation (n) Farmer 69 1, 952 1, 132 306 3, 459 21, 845
    Racial (n) Han Chinese 69 1, 952 1, 132 306 3, 459 21, 845
    Age Mean±SD 15±12 45±13 49±12 51±12 46±14 39±18

    Figure 2.  Prevalence rate of early stage and stages Ⅰ, Ⅱ, and Ⅲ KBD patients distributed among different age groups in Linyou County.

  • We measured the incidence of six clinical symptoms (flexion of the distal part of fingers, deformed fingers, enlarged finger joints, shortened fingers, squat down, and dwarfism) in different groups summarized in Table 3. There were 3, 394 subjects (96.8%) with enlarged finger joints, 1, 609 (46.5%) with flexion of the distal part of fingers, 1, 491 (43.1%) with deformed fingers, 1, 460 (42.2%) with shortened fingers, 1, 212 (35.0%) with squat down, and 315 (9.1%) with dwarfism. The difference in the incidence of six clinical symptoms was significant between the KBD and healthy populations (P < 0.001). The most common clinical symptom was flexion of the distal part of fingers (85.5%) in early stage KBD, enlarged finger joints (97.8%) in stage IKBD, enlarged finger joints (99.4%) in stage Ⅱ KBD, and shortened fingers (99.3%) in stage Ⅲ KBD.

    Table 3.  Incidence of the Six Clinical Symptoms in the Screening Population in Linyou Country

    Groups No. Flexion of Distal
    Part of Fingers (%)
    Deformed
    Fingers (%)
    Enlarged Finger
    Joints (%)
    Shortened Fingers
    (%)
    Squat Down
    (%)
    Dwarfism
    (%)
    Healthy 21, 845 123 (0.6) 135 (0.6) 9 (0) 2 (0) 28 (0.1) 2 (0)
    KBD 3, 459 1, 609 (46.5) 1, 491 (43.1) 3, 349 (96.8) 1, 460 (42.2) 1, 212 (35.0) 315 (9.1)
    Early stage 69 59 (85.5) 34 (49.3) 14 (20.3) 1 (1.4) 5 (7.2) 0 (0.0)
    Ⅰ stage 1, 952 714 (36.6) 613 (31.4) 1, 910 (97.8) 58 (3.0) 296 (15.2) 4 (0.2)
    Ⅱ stage 1, 132 660 (58.3) 658 (58.1) 1, 125 (99.4) 1, 097 (96.9) 618 (54.6) 20 (1.8)
    Ⅲ stage 306 176 (57.5) 186 (60.8) 300 (98.0) 304 (99.3) 293 (95.8) 291 (95.1)
  • Subsequently, we performed a screening test (Table 4) using the new 'Diagnostic Criteria for Kashin-Beck Disease (WS/T207-2010)', which included three clinical symptoms: enlarged finger joints, shortened fingers, and dwarfism. Enlarged finger joints were used to diagnose KBD with a sensitivity, specificity, and Youden index of 0.968, 1.000, and 0.968, respectively. Enlarged finger joints were used to classify stage Ⅰ KBD with a sensitivity, specificity, and Youden index of 0.978, 0.045, and 0.023, respectively. Shortened fingers were used to classify stage Ⅱ KBD with a sensitivity, specificity, and Youden index of 0.969, 0.844, and 0.813, respectively. Dwarfism was used to classify stage Ⅲ KBD, with a sensitivity, specificity and Youden index of 0.951, 0.992, and 0.943, respectively. In a serial test (Table 5), enlarged finger joints without shortened fingers and dwarfism were used to classify stage Ⅰ KBD with a sensitivity, specificity and Youden index of 0.949, 0.967, and 0.916, respectively. Shortened fingers with enlarged finger joints were used to classify stage Ⅱ KBD with a sensitivity, specificity, and Youden index of 0.945, 0.970, and 0.915, respectively. Dwarfism with shortened fingers and enlarged finger joints were used to classify stage Ⅲ KBD with a sensitivity, specificity, and Youden index of 0.925, 0.993, and 0.918, respectively.

    Table 4.  The Sensitivity and Specificity of the Six Clinical Symptoms for the New Clinical Diagnosis and Classification Criterion of Kashin-Beck Disease in a Screening Test

    Clinical Classification Flexion of Distal Part of Fingers Deformed Fingers Enlarged Finger Joints Shortened Fingers Squat Down Dwarfism
    KBD Sensitivity 0.465 0.431 0.968 0.422 0.350 0.091
    Specificity 0.994 0.994 1.000 1.000 0.999 1.000
    Youden's index 0.459 0.425 0.968 0.422 0.349 0.091
    Ⅰ stage Sensitivity 0.366 0.314 0.978 0.030 0.152 0.002
    Specificity 0.406 0.417 0.045 0.070 0.392 0.794
    Youden's index -0.228 -0.269 0.023 -0.900 -0.456 -0.204
    Ⅱ stage Sensitivity 0.583 0.581 0.994 0.969 0.546 0.018
    Specificity 0.592 0.642 0.044 0.844 0.745 0.873
    Youden's index 0.175 0.223 0.038 0.813 0.291 -0.109
    Ⅲ stage Sensitivity 0.575 0.608 0.980 0.993 0.958 0.951
    Specificity 0.546 0.586 0.033 0.633 0.709 0.992
    Youden's index 0.121 0.194 0.013 0.626 0.667 0.943

    Table 5.  A Serial Screening Test of the New Clinical Classification Criterion of Kashin-Beck Disease

    Clinical Classification Sensitivity Specificity Youden's Index
    Ⅰ stage (Enlarged finger joints without shortened fingers and dwarfism) 0.949 0.967 0.916
    Ⅱ stage (Shortened fingers with enlarged finger joints) 0.945 0.970 0.915
    Ⅲ stage (Dwarfism with shortened fingers, enlarged finger joints) 0.925 0.993 0.918

    The 'Diagnostic Criteria for Kashin-Beck Disease (GB16003-1995)' was employed for more than 15 years, and the new version 'Diagnostic Criteria for Kashin-Beck Disease (WS/T207-2010)' was issued in 2010. The new criteria simplified the classification of KBD from four to three stages, creating a simple and practical system. For the screening test in this study, 25, 304 Han Chinese people were sampled and 3, 459 KBD patients were identified using the old criteria (GB16003-1995). Further, we selected KBD patients using the new criteria (WS/T207-2010) with six clinical symptoms. Subsequently, we evaluated the sensitivity and specificity of three clinical symptoms in the clinical classification of KBD using the serial test.

    We identified 3, 459 patients using the old criteria with an average age of 46 years. The proportion of patients with stages Ⅰ, Ⅱ, and Ⅲ KBD was approximately 6:3:1. We selected six clinical symptoms (flexion of the distal part of fingers, deformed fingers, enlarged finger joints, shortened fingers, squat down, and dwarfism) according to the previous studies[2, 17-18]. We identified 96.8% subjects with enlarged finger joints, 46.5% with flexion of distal part of fingers, 43.1% with crooked fingers, 42.2% with short fingers, 35.0% with squat down, and 9.1% with dwarfism. Subsequently, we performed a screening test using the new criteria, including enlarged finger joints, shortened fingers, and dwarfism, to classify stages Ⅰ, Ⅱ, and Ⅲ KBD with high sensitivity. Enlarged finger joints occurring in 96.8% KBD patients was used to classify stage Ⅰ KBD with lower specificity. Therefore, we performed a serial test in which enlarged finger joints without shortened fingers and dwarfism, shortened fingers with enlarged finger joints, and dwarfism with shortened fingers and enlarged finger joints were used to classify stages Ⅰ, Ⅱ, and Ⅲ KBD, respectively, with high sensitivity and specificity.

    The new diagnostic criteria (WS/T207-2010) represent a simplified version of the old diagnostic criteria (GB16003-1995) with higher sensitivity and specificity than the old diagnostic criteria (Table 1). The four stages of classification in the old diagnostic criteria was revised to three stages in the new diagnosis criteria, and the new diagnostic criteria also removed some of the clinical signs from the old diagnostic criteria (Table 1). Therefore, the new diagnostic criteria are convenient for epidemiological surveillance and have extensive clinical applications.

    This study has two limitations. First, the new diagnostic criteria were used to screen KBD patients in endemic areas. The old diagnostic criteria were also applicable to only endemic areas and excluded other areas, and the screening test in the new diagnostic criteria was based on the old diagnostic criteria. Second, screening personnel required professional training to adequately perform the epidemiological surveillance of KBD patients. Therefore, the new diagnostic criteria can be used to screen endemic population only by professionally trained screening personnel.

    In summary, we identified 3, 459 KBD patients, of which 69 were classified as early stage KBD, 1, 952 as stage Ⅰ KBD, 1, 132 as stage Ⅱ KBD, and 306 as stage Ⅲ KBD. The screening and serial tests revealed that enlarged finger joints, shortened fingers, and dwarfism can be used to classify stages Ⅰ, Ⅱ, and Ⅲ KBD with high sensitivity ( > 0.9) and specificity ( > 0.9).

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