Multi-drug Resistance and Characteristic of Integrons in Shigella spp.Isolated from China

ZHU JingYuan; DUAN GuangCai; YANG HaiYan; FAN QingTang; XI YuanLin

doi:10.3967/0895-3988.2011.01.007

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Volume 24 Issue 1

Feb. 2011

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Article Navigation > Biomedical and Environmental Sciences > 2011 > 24(1): 56-61

ZHU JingYuan, DUAN GuangCai, YANG HaiYan, FAN QingTang, XI YuanLin. Multi-drug Resistance and Characteristic of Integrons in Shigella spp.Isolated from China[J]. Biomedical and Environmental Sciences, 2011, 24(1): 56-61. doi: 10.3967/0895-3988.2011.01.007

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ZHU JingYuan, DUAN GuangCai, YANG HaiYan, FAN QingTang, XI YuanLin. Multi-drug Resistance and Characteristic of Integrons in Shigella spp.Isolated from China[J]. Biomedical and Environmental Sciences, 2011, 24(1): 56-61. doi: 10.3967/0895-3988.2011.01.007

Multi-drug Resistance and Characteristic of Integrons in Shigella spp.Isolated from China

doi: 10.3967/0895-3988.2011.01.007

Department of Environmental Health, School of Public Health, Zhengzhou University, Zhengzhou 450001,Henan China
Department of Epidemiology, School of Public Health, Zhengzhou University, Zhengzhou 450001, Henan China;Henan University of Science & Technology, Luoyang 471003, Henan, China
Department of Epidemiology, School of Public Health, Zhengzhou University, Zhengzhou 450001, Henan China
The Key Laboratory of Molecular Medicine in Henan Province, Zhengzhou 450052, Henan, China

Funds: the Research Fund from Ministry of Health of the People's Republic of China(WKJ2007-2-024)

Abstract

Objective To investigate the characteristic of integrons and the relationship between integrons and antimicrobial resistance in Shigellaspp.Methods Ninety 5higella strains (83 S. flexneri and 7 S. sonnel) were isolated from the stools of patients in China. Susceptibility to 8 antimicrobials was tested for all isolated strains. PCR, RFLP and sequencing analysis of integrons were applied to all of them.Results High prevalence of multi-drug resistance (95.6%) was identified. Of the isolates 79 (87.8%)carried integrase genes of class 1 integron (3.3%), class 2 integron (10.0%) or both (74.4%). No intβ was detected in the tested isolates. The prevalence of intΩ was significantly higher in isolates with multi-drug resistance to at least 3 antibiotics than that in isolates with resistance to 2 and less antibiotics (P＜0.05). Gene cassettes dfrA17-aadAS, dfrA12-orfF-aadA2 of class 1 integron and dfrA1-sat1-aadA1 of class 2 integron were identified.Conclusion The class 2 integron may play a role in the emergence of multi-drug resistance in Shigella spp.
- Resistance,
- Shigella flexneri,
- Integron,
- Gene cassettes
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Multi-drug Resistance and Characteristic of Integrons in Shigella spp.Isolated from China

doi: 10.3967/0895-3988.2011.01.007

Department of Environmental Health, School of Public Health, Zhengzhou University, Zhengzhou 450001,Henan China

Department of Epidemiology, School of Public Health, Zhengzhou University, Zhengzhou 450001, Henan China;Henan University of Science & Technology, Luoyang 471003, Henan, China

Department of Epidemiology, School of Public Health, Zhengzhou University, Zhengzhou 450001, Henan China

The Key Laboratory of Molecular Medicine in Henan Province, Zhengzhou 450052, Henan, China

Funds: the Research Fund from Ministry of Health of the People's Republic of China(WKJ2007-2-024)

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Abstract: Objective To investigate the characteristic of integrons and the relationship between integrons and antimicrobial resistance in Shigellaspp.Methods Ninety 5higella strains (83 S. flexneri and 7 S. sonnel) were isolated from the stools of patients in China. Susceptibility to 8 antimicrobials was tested for all isolated strains. PCR, RFLP and sequencing analysis of integrons were applied to all of them.Results High prevalence of multi-drug resistance (95.6%) was identified. Of the isolates 79 (87.8%)carried integrase genes of class 1 integron (3.3%), class 2 integron (10.0%) or both (74.4%). No intβ was detected in the tested isolates. The prevalence of intΩ was significantly higher in isolates with multi-drug resistance to at least 3 antibiotics than that in isolates with resistance to 2 and less antibiotics (P＜0.05). Gene cassettes dfrA17-aadAS, dfrA12-orfF-aadA2 of class 1 integron and dfrA1-sat1-aadA1 of class 2 integron were identified.Conclusion The class 2 integron may play a role in the emergence of multi-drug resistance in Shigella spp.

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