Gene Expression Changes in the Pituitary Gland of Rats Exposed to Electromagnetic Pulses

QI YuHong; LIANG Jun; HUI YanPing; DING GuiRong; LIU JunYe; SU XiaoMing; GUO GuoZhen

doi:10.3967/0895-3988.2011.01.016

PDF
Cite
Share

facebook

twitter

google

linkedin

Volume 24 Issue 5

Oct. 2011

Turn off MathJax

Article Contents

Article Navigation > Biomedical and Environmental Sciences > 2011 > 24(5): 560-568

QI YuHong, LIANG Jun, HUI YanPing, DING GuiRong, LIU JunYe, SU XiaoMing, GUO GuoZhen. Gene Expression Changes in the Pituitary Gland of Rats Exposed to Electromagnetic Pulses[J]. Biomedical and Environmental Sciences, 2011, 24(5): 560-568. doi: 10.3967/0895-3988.2011.01.016

Citation:

QI YuHong, LIANG Jun, HUI YanPing, DING GuiRong, LIU JunYe, SU XiaoMing, GUO GuoZhen. Gene Expression Changes in the Pituitary Gland of Rats Exposed to Electromagnetic Pulses[J]. Biomedical and Environmental Sciences, 2011, 24(5): 560-568. doi: 10.3967/0895-3988.2011.01.016

Gene Expression Changes in the Pituitary Gland of Rats Exposed to Electromagnetic Pulses

doi: 10.3967/0895-3988.2011.01.016

Department of Radiotherapy, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, Shaanxi,China;Department of Radiation Medicine, Faculty of Preventive Medicine, Fourth Military Medical University,Xi'an 710032, Shaanxi, China
Department of Radiotherapy, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, Shaanxi,China
Department of Pathology, Faculty of Preclinical Medicine, Fourth Military Medical University, Xi 'an 710032, Shaanxi, China

Funds: This study was supported by the Research Fund of the National Natural Science Foundation of China(No:60871068%30970670)

Abstract

Objective We examined alterations in the expression of tumorigenesis-related genes in the pituitary gland of rats exposed to electromagnetic pulses (EMP).Methods The global gene expression profiles of the pituitary gland in EMP-exposed and control groups were detected by cDNA microarray analysis.We then validated and further investigated the reduced expression of two tumorigenesis-related genes,Pten,and Jund,by assessing their mRNA and protein expression by quantitative real-time-PCR,western blotting,and immunohistochemistry in the pituitary gland of rats 6 months after exposure to EMP.Results EMP exposure induced genome-wide gene expression changes in the rat pituitary gland.There was decreased expression of the Pten and Jund mRNAs and proteins in EMP-exposed rats compared with in unexposed control animals.Conclusion EMP exposure alters the expression of tumorigenesis-related genes in the pituitary gland.These tumorigenesis-related genes are potentially involved in the development of pituitary gland tumors in rats.
- Tumorigenesis,
- Electromagnetic pulses,
- Pituitary gland,
- Pten,
- Jund
References
Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article Metrics

Article views(1488) PDF downloads(18) Cited by()

Proportional views

Gene Expression Changes in the Pituitary Gland of Rats Exposed to Electromagnetic Pulses

doi: 10.3967/0895-3988.2011.01.016

Department of Radiotherapy, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, Shaanxi,China;Department of Radiation Medicine, Faculty of Preventive Medicine, Fourth Military Medical University,Xi'an 710032, Shaanxi, China

Department of Radiotherapy, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, Shaanxi,China

Department of Pathology, Faculty of Preclinical Medicine, Fourth Military Medical University, Xi 'an 710032, Shaanxi, China

Funds: This study was supported by the Research Fund of the National Natural Science Foundation of China(No:60871068%30970670)

Key words:

Abstract: Objective We examined alterations in the expression of tumorigenesis-related genes in the pituitary gland of rats exposed to electromagnetic pulses (EMP).Methods The global gene expression profiles of the pituitary gland in EMP-exposed and control groups were detected by cDNA microarray analysis.We then validated and further investigated the reduced expression of two tumorigenesis-related genes,Pten,and Jund,by assessing their mRNA and protein expression by quantitative real-time-PCR,western blotting,and immunohistochemistry in the pituitary gland of rats 6 months after exposure to EMP.Results EMP exposure induced genome-wide gene expression changes in the rat pituitary gland.There was decreased expression of the Pten and Jund mRNAs and proteins in EMP-exposed rats compared with in unexposed control animals.Conclusion EMP exposure alters the expression of tumorigenesis-related genes in the pituitary gland.These tumorigenesis-related genes are potentially involved in the development of pituitary gland tumors in rats.

Citation:

QI YuHong, LIANG Jun, HUI YanPing, DING GuiRong, LIU JunYe, SU XiaoMing, GUO GuoZhen. Gene Expression Changes in the Pituitary Gland of Rats Exposed to Electromagnetic Pulses[J]. Biomedical and Environmental Sciences, 2011, 24(5): 560-568. doi: 10.3967/0895-3988.2011.01.016