Methylation of RAR-β2, RASSF1A, and CDKN2A Genes Induced by Nickel Subsulfide and Nickel-carcinogenesis in Rats

ZHANG Jun; ZHANG Jing; LI MengJie; Wu YouJun; FAN YingYing; ZHOU Yang; TAN LiLi; SHAO ZhiHua; SHI HongJun

doi:10.3967/0895-3988.2011.02.011

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Volume 24 Issue 2

Apr. 2011

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Article Navigation > Biomedical and Environmental Sciences > 2011 > 24(2): 163-171

ZHANG Jun, ZHANG Jing, LI MengJie, Wu YouJun, FAN YingYing, ZHOU Yang, TAN LiLi, SHAO ZhiHua, SHI HongJun. Methylation of RAR-β2, RASSF1A, and CDKN2A Genes Induced by Nickel Subsulfide and Nickel-carcinogenesis in Rats[J]. Biomedical and Environmental Sciences, 2011, 24(2): 163-171. doi: 10.3967/0895-3988.2011.02.011

Citation:

ZHANG Jun, ZHANG Jing, LI MengJie, Wu YouJun, FAN YingYing, ZHOU Yang, TAN LiLi, SHAO ZhiHua, SHI HongJun. Methylation of RAR-β2, RASSF1A, and CDKN2A Genes Induced by Nickel Subsulfide and Nickel-carcinogenesis in Rats[J]. Biomedical and Environmental Sciences, 2011, 24(2): 163-171. doi: 10.3967/0895-3988.2011.02.011

Methylation of RAR-β2, RASSF1A, and CDKN2A Genes Induced by Nickel Subsulfide and Nickel-carcinogenesis in Rats

doi: 10.3967/0895-3988.2011.02.011

Department of Regenerative Medicine, Tongji University School of Medicine, Shanghai 200092, China
School of Life Science and Technology, Tongji University, Shanghai 200092, China

Funds: the Natural Science Foundation of China(30570690%81041069)%the Natural Science Foundation of Shanghai(08ZR1420700)

Abstract

Objective To investigate the expression variation of RAR-β2, RASSF1A, and CDKN2A gene in the process of nickel-induced carcinogenesis. Methods Nickel subsulfide (Ni<,3>S<,2>) at dose of 10 mg was given to Wistar rats by intramuscular injection. The mRNA expression of the three genes in induced tumors and their lung metastasis were examined by Real-time PCR. The methylation status of the 5' region of these genes were detected by Quantitative Real-time methylation specific PCR. Results The mRNA expressions of the three genes both in muscle and lung tumor were decreased distinctly in comparison with normal tissue. But hypermethylation was found only in muscle tumor. Conclusion These findings suggest that loss of function or decrease of RAR-β2, RASSF1A, and CDKN2A, as well as the hypermethylation of 5' region of these genes, are related with nickel exposure.
- Nickel,
- DNA methylation,
- RAR-β2,
- RASSF1A,
- CDKN2A,
- Quantitative Real-time Methylation Specific PCR
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Methylation of RAR-β2, RASSF1A, and CDKN2A Genes Induced by Nickel Subsulfide and Nickel-carcinogenesis in Rats

doi: 10.3967/0895-3988.2011.02.011

Department of Regenerative Medicine, Tongji University School of Medicine, Shanghai 200092, China

School of Life Science and Technology, Tongji University, Shanghai 200092, China

Funds: the Natural Science Foundation of China(30570690%81041069)%the Natural Science Foundation of Shanghai(08ZR1420700)

Key words:

Nickel /
DNA methylation /
RAR-β2 /
RASSF1A /
CDKN2A /
Quantitative Real-time Methylation Specific PCR

Abstract: Objective To investigate the expression variation of RAR-β2, RASSF1A, and CDKN2A gene in the process of nickel-induced carcinogenesis. Methods Nickel subsulfide (Ni<,3>S<,2>) at dose of 10 mg was given to Wistar rats by intramuscular injection. The mRNA expression of the three genes in induced tumors and their lung metastasis were examined by Real-time PCR. The methylation status of the 5' region of these genes were detected by Quantitative Real-time methylation specific PCR. Results The mRNA expressions of the three genes both in muscle and lung tumor were decreased distinctly in comparison with normal tissue. But hypermethylation was found only in muscle tumor. Conclusion These findings suggest that loss of function or decrease of RAR-β2, RASSF1A, and CDKN2A, as well as the hypermethylation of 5' region of these genes, are related with nickel exposure.

Citation:

ZHANG Jun, ZHANG Jing, LI MengJie, Wu YouJun, FAN YingYing, ZHOU Yang, TAN LiLi, SHAO ZhiHua, SHI HongJun. Methylation of RAR-β2, RASSF1A, and CDKN2A Genes Induced by Nickel Subsulfide and Nickel-carcinogenesis in Rats[J]. Biomedical and Environmental Sciences, 2011, 24(2): 163-171. doi: 10.3967/0895-3988.2011.02.011