Volume 24 Issue 4
Aug.  2011
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WANG Jing, LV XiaoWen, SHI JiePing, HU XiaoSong, DU YuGuo. Troglitazone Induced Apoptosis via PPARy Activated POX-induced ROS Formation in HT29 Cells[J]. Biomedical and Environmental Sciences, 2011, 24(4): 391-399. doi: 10.3967/0895-3988.2011.04.010
Citation: WANG Jing, LV XiaoWen, SHI JiePing, HU XiaoSong, DU YuGuo. Troglitazone Induced Apoptosis via PPARy Activated POX-induced ROS Formation in HT29 Cells[J]. Biomedical and Environmental Sciences, 2011, 24(4): 391-399. doi: 10.3967/0895-3988.2011.04.010

Troglitazone Induced Apoptosis via PPARy Activated POX-induced ROS Formation in HT29 Cells

doi: 10.3967/0895-3988.2011.04.010
Funds:  the National Basic Research Program (973) of China(2009CB421605%2008CB418102)%the National Natural Science Foundation of China(20890112%21077127)
  • Objective In order to investigate the potential mechanisms in troglitazone-induced apoptosis in HT29 cells,the effects of PPARY and POX-induced ROS were explored.Methods [3-(4,5)-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay,Annexin V and PI staining using FACS,plasmid transfection,ROS formation detected by DCFH staining,RNA interference,RT-PCR & RT-QPCR,and Western blotting analyses were employed to investigate the apoptotic effect of troglitazone and the potential role of PPARY pathway and POX-induced ROS formation in HT29 cells.Results Troglitazone was found to inhibit the growth of HT29 cells by induction of apoptosis.During this process,mitochondria related pathways including ROS formation,POX expression and cytochrome c release increased,which were inhibited by pretreatment with GW9662,a specific antagonist of PPARY.These results illustrated that POX upregulation and ROS formation in apoptosis induced by troglitazone was modulated in PPARY-dependent pattern.Furthermore,the inhibition of ROS and apoptosis after POX siRNA used in troglitazone-treated HT29 cells indicated that POX be essential in the ROS formation and PPARy-dependent apoptosis induced by troglitazone.Conclusion The findings from this study showed that troglitazone-induced apoptosis was mediated by POX-induced ROS formation,at least partly,via PPARY activation.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Troglitazone Induced Apoptosis via PPARy Activated POX-induced ROS Formation in HT29 Cells

doi: 10.3967/0895-3988.2011.04.010
Funds:  the National Basic Research Program (973) of China(2009CB421605%2008CB418102)%the National Natural Science Foundation of China(20890112%21077127)

Abstract: Objective In order to investigate the potential mechanisms in troglitazone-induced apoptosis in HT29 cells,the effects of PPARY and POX-induced ROS were explored.Methods [3-(4,5)-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay,Annexin V and PI staining using FACS,plasmid transfection,ROS formation detected by DCFH staining,RNA interference,RT-PCR & RT-QPCR,and Western blotting analyses were employed to investigate the apoptotic effect of troglitazone and the potential role of PPARY pathway and POX-induced ROS formation in HT29 cells.Results Troglitazone was found to inhibit the growth of HT29 cells by induction of apoptosis.During this process,mitochondria related pathways including ROS formation,POX expression and cytochrome c release increased,which were inhibited by pretreatment with GW9662,a specific antagonist of PPARY.These results illustrated that POX upregulation and ROS formation in apoptosis induced by troglitazone was modulated in PPARY-dependent pattern.Furthermore,the inhibition of ROS and apoptosis after POX siRNA used in troglitazone-treated HT29 cells indicated that POX be essential in the ROS formation and PPARy-dependent apoptosis induced by troglitazone.Conclusion The findings from this study showed that troglitazone-induced apoptosis was mediated by POX-induced ROS formation,at least partly,via PPARY activation.

WANG Jing, LV XiaoWen, SHI JiePing, HU XiaoSong, DU YuGuo. Troglitazone Induced Apoptosis via PPARy Activated POX-induced ROS Formation in HT29 Cells[J]. Biomedical and Environmental Sciences, 2011, 24(4): 391-399. doi: 10.3967/0895-3988.2011.04.010
Citation: WANG Jing, LV XiaoWen, SHI JiePing, HU XiaoSong, DU YuGuo. Troglitazone Induced Apoptosis via PPARy Activated POX-induced ROS Formation in HT29 Cells[J]. Biomedical and Environmental Sciences, 2011, 24(4): 391-399. doi: 10.3967/0895-3988.2011.04.010

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