Orthogonal Test Design for Optimization of the Extraction of Flavonid from the Fructus Gardeniae

CAI Ding Jian; SHU Qing; XU Bao Quan; PENG Li Mei; HE Yan

doi:10.3967/0895-3988.2011.06.015

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Volume 24 Issue 6

Dec. 2011

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Article Navigation > Biomedical and Environmental Sciences > 2011 > 24(6): 688-693

CAI Ding Jian, SHU Qing, XU Bao Quan, PENG Li Mei, HE Yan. Orthogonal Test Design for Optimization of the Extraction of Flavonid from the Fructus Gardeniae[J]. Biomedical and Environmental Sciences, 2011, 24(6): 688-693. doi: 10.3967/0895-3988.2011.06.015

Citation:

CAI Ding Jian, SHU Qing, XU Bao Quan, PENG Li Mei, HE Yan. Orthogonal Test Design for Optimization of the Extraction of Flavonid from the Fructus Gardeniae[J]. Biomedical and Environmental Sciences, 2011, 24(6): 688-693. doi: 10.3967/0895-3988.2011.06.015

Orthogonal Test Design for Optimization of the Extraction of Flavonid from the Fructus Gardeniae

doi: 10.3967/0895-3988.2011.06.015

School of Metallurgy and Chemical Engineering, Jiangxi University of Science and Technology, Ganzhou 341000,Jiangxi, China

Funds: National Natural Science Foundation of China(21067004)%Research Fund for the Doctoral Program of Jiangxi University of Science and Technology(22020)

Abstract

Objective It is imperative to provide some consistent experimental results for the extraction of flavonid from Fructus Gardeniae.Methods The key extraction parameters that influenced the yield of flavonid from Fructus Gardeniae were optimized by employing an orthogonal experiment [L9(3)4],including the ratio of buffer solution (Na2B4O7 · 10H2O) to raw material,concentration of Fructus Gardeniae in extracting solution,extraction time and pH of buffer solution.An UV/Vis detector was used to perform the qualitative and quantitative analyses of the extracted flavonid with the using of the standard sample.Results The maximum extraction yield of the crude extract was 5.0533 (mg/g) after 20 min when the mass ratio of Na2B4O7 · 10H2O to raw material was 0.4％,the concentration of Fructus Gardeniae in the extraction solution was 1/12 (g/mL),and pH of buffer solution was 4.5.The positive reactions to the Molish and HCI-Mg tests suggested that the extracted compound was flavonoid,and FTIR measurements also identified the presence of flavonoid in the extracts.Conclusion This work is expected to provide a basis for further research,development,and utilization of Fructus gardenia in flavonid extraction.
- Fructus Gardeniae,
- Flavonid,
- Extraction,
- Orthogonal experiment
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Orthogonal Test Design for Optimization of the Extraction of Flavonid from the Fructus Gardeniae

doi: 10.3967/0895-3988.2011.06.015

School of Metallurgy and Chemical Engineering, Jiangxi University of Science and Technology, Ganzhou 341000,Jiangxi, China

Funds: National Natural Science Foundation of China(21067004)%Research Fund for the Doctoral Program of Jiangxi University of Science and Technology(22020)

Key words:

Abstract: Objective It is imperative to provide some consistent experimental results for the extraction of flavonid from Fructus Gardeniae.Methods The key extraction parameters that influenced the yield of flavonid from Fructus Gardeniae were optimized by employing an orthogonal experiment [L9(3)4],including the ratio of buffer solution (Na2B4O7 · 10H2O) to raw material,concentration of Fructus Gardeniae in extracting solution,extraction time and pH of buffer solution.An UV/Vis detector was used to perform the qualitative and quantitative analyses of the extracted flavonid with the using of the standard sample.Results The maximum extraction yield of the crude extract was 5.0533 (mg/g) after 20 min when the mass ratio of Na2B4O7 · 10H2O to raw material was 0.4％,the concentration of Fructus Gardeniae in the extraction solution was 1/12 (g/mL),and pH of buffer solution was 4.5.The positive reactions to the Molish and HCI-Mg tests suggested that the extracted compound was flavonoid,and FTIR measurements also identified the presence of flavonoid in the extracts.Conclusion This work is expected to provide a basis for further research,development,and utilization of Fructus gardenia in flavonid extraction.

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