In vitro Study of Nucleostemin Gene as a Potential Therapeutic Target for Human Lung Carcinoma

GAO Hong Xia; GAO Xiu Feng; WANG Guo Qing; WANG En Shu; HUANG Wei; HUANG Ping

doi:10.3967/0895-3988.2012.01.013

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Volume 25 Issue 1

Feb. 2012

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Article Navigation > Biomedical and Environmental Sciences > 2012 > 25(1): 91-97

GAO Hong Xia, GAO Xiu Feng, WANG Guo Qing, WANG En Shu, HUANG Wei, HUANG Ping. In vitro Study of Nucleostemin Gene as a Potential Therapeutic Target for Human Lung Carcinoma[J]. Biomedical and Environmental Sciences, 2012, 25(1): 91-97. doi: 10.3967/0895-3988.2012.01.013

Citation:

GAO Hong Xia, GAO Xiu Feng, WANG Guo Qing, WANG En Shu, HUANG Wei, HUANG Ping. In vitro Study of Nucleostemin Gene as a Potential Therapeutic Target for Human Lung Carcinoma[J]. Biomedical and Environmental Sciences, 2012, 25(1): 91-97. doi: 10.3967/0895-3988.2012.01.013

In vitro Study of Nucleostemin Gene as a Potential Therapeutic Target for Human Lung Carcinoma

doi: 10.3967/0895-3988.2012.01.013

Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine,Sichuan University, Chengdu 610041, Sichuan, China
Institute of Laboratory Medicine, Beihua University, Jilin 132013, Jilin, China
College of Communication Engineering, Jilin University, Changchun 130012, Jilin,China

Funds: This research was supported by the Plan of Promoting Sichuan University science research start up fund(0082204127092)

Abstract

Objective Nucleostemin (NS) is a GTP-conjugated protein located in the nucleoli of stem cells and some cancer cells,and maintains cell self-renewal.We aimed to evaluate NS as a potential target for lung carcinoma gene therapy by investigating NS gene expression and its effect on A549 cell proliferation.Methods NS mRNA and protein expression in A549,HepG2,SMMC-7721,HeLa,and U251 cells was analyzed by RT-PCR and western blotting following transfection of NS siRNAs and negative control siRNA (NC).The effect on cell proliferation was also analyzed by MTT assays.Results NS mRNA and protein were both expressed in A549 cells and four other tumor cell lines; the relative expression levels were similar in all five cell lines.The three pairs of NS siRNA,either transfected alone or cotransfected into A549 cells,could effectively inhibit the expression of NS mRNA and protein.Moreover,the interference ratio showed an obvious concentration-dependent relationship.NS siRNA treatment resulted in significant inhibition of A549 cell proliferation by 35.7％.Conclusion NS gene was not only highly expressed but also played an important role in A549 cell proliferation.Thus,targeting of NS may be a promising novel strategy for the treatment of lung carcinoma.
- Nucleostemin,
- RNA interference,
- A549 cells,
- Tumor gene therapy
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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In vitro Study of Nucleostemin Gene as a Potential Therapeutic Target for Human Lung Carcinoma

doi: 10.3967/0895-3988.2012.01.013

Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine,Sichuan University, Chengdu 610041, Sichuan, China

Institute of Laboratory Medicine, Beihua University, Jilin 132013, Jilin, China

College of Communication Engineering, Jilin University, Changchun 130012, Jilin,China

Funds: This research was supported by the Plan of Promoting Sichuan University science research start up fund(0082204127092)

Key words:

Abstract: Objective Nucleostemin (NS) is a GTP-conjugated protein located in the nucleoli of stem cells and some cancer cells,and maintains cell self-renewal.We aimed to evaluate NS as a potential target for lung carcinoma gene therapy by investigating NS gene expression and its effect on A549 cell proliferation.Methods NS mRNA and protein expression in A549,HepG2,SMMC-7721,HeLa,and U251 cells was analyzed by RT-PCR and western blotting following transfection of NS siRNAs and negative control siRNA (NC).The effect on cell proliferation was also analyzed by MTT assays.Results NS mRNA and protein were both expressed in A549 cells and four other tumor cell lines; the relative expression levels were similar in all five cell lines.The three pairs of NS siRNA,either transfected alone or cotransfected into A549 cells,could effectively inhibit the expression of NS mRNA and protein.Moreover,the interference ratio showed an obvious concentration-dependent relationship.NS siRNA treatment resulted in significant inhibition of A549 cell proliferation by 35.7％.Conclusion NS gene was not only highly expressed but also played an important role in A549 cell proliferation.Thus,targeting of NS may be a promising novel strategy for the treatment of lung carcinoma.

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