Volume 28 Issue 10
Oct.  2015
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GUAN Li, LI Zong Yang, ZHANG Yan Lin, CONG Cui Cui, ZHAO Jin Yuan. Early Biomarkers in 1H Nuclear Magnetic Resonance Spectroscopy of Striatal Pathological Mechanisms after Acute Carbon Monoxide Poisoning in Rats[J]. Biomedical and Environmental Sciences, 2015, 28(10): 728-737. doi: 10.3967/bes2015.103
Citation: GUAN Li, LI Zong Yang, ZHANG Yan Lin, CONG Cui Cui, ZHAO Jin Yuan. Early Biomarkers in 1H Nuclear Magnetic Resonance Spectroscopy of Striatal Pathological Mechanisms after Acute Carbon Monoxide Poisoning in Rats[J]. Biomedical and Environmental Sciences, 2015, 28(10): 728-737. doi: 10.3967/bes2015.103

Early Biomarkers in 1H Nuclear Magnetic Resonance Spectroscopy of Striatal Pathological Mechanisms after Acute Carbon Monoxide Poisoning in Rats

doi: 10.3967/bes2015.103
Funds:  This research was supported by the National Natural Science Foundation of China,‘Study on the pathogenic effect of HO-1/CO in the delayed encephalopathy after acute CO poisoning’,(Grant 81101024)
  • Objective In vivo Proton Magnetic Resonance Spectroscopy (1H-MRS) can be used to evaluate the levels of specific neurochemical biomarkers of pathological mechanisms in the brain.
    Methods We conducted T2-Weighted Magnetic Resonance Imaging (MRI) and 1H-MRS with a 3.0-Tesla animal MRI system to investigate the early microstructural and metabolic profiles in vivo in the striatum of rats following carbon monoxide (CO) poisoning.
    Results Compared to baseline, we found significant cortical surface deformation, cerebral edema changes, which were indicated by the unclear gray/white matter border, and lateral ventricular volume changes in the brain. A significant reduction in the metabolite to total creatine (Cr) ratios of N-acetylaspartate (NAA) was observed as early as 1 h after the last CO administration, while the lactate (Lac) levels increased marginally. Both the Lac/Cr and NAA/Cr ratios leveled off at 6 h and showed no subsequent significant changes. In addition, compared to the control, the choline (Cho)/Cr ratio was slightly reduced in the early stages and significantly increased after 6 h. In addition, a pathological examination revealed mild cerebral edema on cessation of the insult and more severe cerebral injury after additional CO poisoning.
    Conclusion The present study demonstrated that 1H-MRS of the brain identified early metabolic changes after CO poisoning. Notably, the relationship between the increased Cho/Cr ratio in the striatum and delayed neuropsychologic sequelae requires further research.
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Early Biomarkers in 1H Nuclear Magnetic Resonance Spectroscopy of Striatal Pathological Mechanisms after Acute Carbon Monoxide Poisoning in Rats

doi: 10.3967/bes2015.103
Funds:  This research was supported by the National Natural Science Foundation of China,‘Study on the pathogenic effect of HO-1/CO in the delayed encephalopathy after acute CO poisoning’,(Grant 81101024)

Abstract: Objective In vivo Proton Magnetic Resonance Spectroscopy (1H-MRS) can be used to evaluate the levels of specific neurochemical biomarkers of pathological mechanisms in the brain.
Methods We conducted T2-Weighted Magnetic Resonance Imaging (MRI) and 1H-MRS with a 3.0-Tesla animal MRI system to investigate the early microstructural and metabolic profiles in vivo in the striatum of rats following carbon monoxide (CO) poisoning.
Results Compared to baseline, we found significant cortical surface deformation, cerebral edema changes, which were indicated by the unclear gray/white matter border, and lateral ventricular volume changes in the brain. A significant reduction in the metabolite to total creatine (Cr) ratios of N-acetylaspartate (NAA) was observed as early as 1 h after the last CO administration, while the lactate (Lac) levels increased marginally. Both the Lac/Cr and NAA/Cr ratios leveled off at 6 h and showed no subsequent significant changes. In addition, compared to the control, the choline (Cho)/Cr ratio was slightly reduced in the early stages and significantly increased after 6 h. In addition, a pathological examination revealed mild cerebral edema on cessation of the insult and more severe cerebral injury after additional CO poisoning.
Conclusion The present study demonstrated that 1H-MRS of the brain identified early metabolic changes after CO poisoning. Notably, the relationship between the increased Cho/Cr ratio in the striatum and delayed neuropsychologic sequelae requires further research.

GUAN Li, LI Zong Yang, ZHANG Yan Lin, CONG Cui Cui, ZHAO Jin Yuan. Early Biomarkers in 1H Nuclear Magnetic Resonance Spectroscopy of Striatal Pathological Mechanisms after Acute Carbon Monoxide Poisoning in Rats[J]. Biomedical and Environmental Sciences, 2015, 28(10): 728-737. doi: 10.3967/bes2015.103
Citation: GUAN Li, LI Zong Yang, ZHANG Yan Lin, CONG Cui Cui, ZHAO Jin Yuan. Early Biomarkers in 1H Nuclear Magnetic Resonance Spectroscopy of Striatal Pathological Mechanisms after Acute Carbon Monoxide Poisoning in Rats[J]. Biomedical and Environmental Sciences, 2015, 28(10): 728-737. doi: 10.3967/bes2015.103

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