Volume 29 Issue 4
Apr.  2016
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Fatma Guesmi, Hamida Beghalem, Amit K Tyagi, Manel Ben Ali, Ramla Ben Mouhoub, Houda Bellamine, Ahmed Landoulsi. Prevention of H2O2 Induced Oxidative Damages of Rat Testis by Thymus algeriensis[J]. Biomedical and Environmental Sciences, 2016, 29(4): 275-285. doi: 10.3967/bes2016.035
Citation: Fatma Guesmi, Hamida Beghalem, Amit K Tyagi, Manel Ben Ali, Ramla Ben Mouhoub, Houda Bellamine, Ahmed Landoulsi. Prevention of H2O2 Induced Oxidative Damages of Rat Testis by Thymus algeriensis[J]. Biomedical and Environmental Sciences, 2016, 29(4): 275-285. doi: 10.3967/bes2016.035

Prevention of H2O2 Induced Oxidative Damages of Rat Testis by Thymus algeriensis

doi: 10.3967/bes2016.035
  • ObjectiveWe evaluate the effects ofThymus algeriensis (TEO) against hydrogen peroxide (H2O2) toxicity on body and testis weight, testis sperm count, testis lipid peroxidation, and antioxidant enzyme activities in rats.
    MethodsRats were treated with low (LD) and high dose (HD) of H2O2 (0.1 and 1 mmol/L) in the presence or absence of TEO (150 mg/kg).
    ResultsThe results exhibited a significant decrease in body weight and testis weight, in total sperm number decrease (P<0.05), sperm motility and percentage of sperm viability, leading to complete arrest, in sperm flagellar beat frequency by the gavage of 1 mmol/L H2O2 compared to controls.The administration of H2O2 resulted in a significant reduction in testis GSH, GPx, CAT, SOD, and GST activity andsignificant increase (P<0.05) in MDAconcentration compared with the untreated control animals. TEO pre-treatment protected testis from the H2O2generated oxidative stress.These results were confirmed byhistological architecture examinations.
    ConclusionH2O2 has the ability to alter the sperm function, characteristics and development of testis. However, TEO is an efficient natural agent, which can prevent the testis from H2O2-induced oxidative damage in rats.
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Prevention of H2O2 Induced Oxidative Damages of Rat Testis by Thymus algeriensis

doi: 10.3967/bes2016.035

Abstract: ObjectiveWe evaluate the effects ofThymus algeriensis (TEO) against hydrogen peroxide (H2O2) toxicity on body and testis weight, testis sperm count, testis lipid peroxidation, and antioxidant enzyme activities in rats.
MethodsRats were treated with low (LD) and high dose (HD) of H2O2 (0.1 and 1 mmol/L) in the presence or absence of TEO (150 mg/kg).
ResultsThe results exhibited a significant decrease in body weight and testis weight, in total sperm number decrease (P<0.05), sperm motility and percentage of sperm viability, leading to complete arrest, in sperm flagellar beat frequency by the gavage of 1 mmol/L H2O2 compared to controls.The administration of H2O2 resulted in a significant reduction in testis GSH, GPx, CAT, SOD, and GST activity andsignificant increase (P<0.05) in MDAconcentration compared with the untreated control animals. TEO pre-treatment protected testis from the H2O2generated oxidative stress.These results were confirmed byhistological architecture examinations.
ConclusionH2O2 has the ability to alter the sperm function, characteristics and development of testis. However, TEO is an efficient natural agent, which can prevent the testis from H2O2-induced oxidative damage in rats.

Fatma Guesmi, Hamida Beghalem, Amit K Tyagi, Manel Ben Ali, Ramla Ben Mouhoub, Houda Bellamine, Ahmed Landoulsi. Prevention of H2O2 Induced Oxidative Damages of Rat Testis by Thymus algeriensis[J]. Biomedical and Environmental Sciences, 2016, 29(4): 275-285. doi: 10.3967/bes2016.035
Citation: Fatma Guesmi, Hamida Beghalem, Amit K Tyagi, Manel Ben Ali, Ramla Ben Mouhoub, Houda Bellamine, Ahmed Landoulsi. Prevention of H2O2 Induced Oxidative Damages of Rat Testis by Thymus algeriensis[J]. Biomedical and Environmental Sciences, 2016, 29(4): 275-285. doi: 10.3967/bes2016.035

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