Volume 29 Issue 8
Aug.  2016
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DU Ying, YANG Sheng Hua, LI Sha, CUI ChuanJue, ZHANG Yan, ZHUChengGang, GUO YuanLin, WU NaQiong, GAO Ying, SUN Jing, DONG Qian, LIU Geng, LI JianJun. Circulating MicroRNAs as Novel Diagnostic Biomarkers for Very Early-onset (≤40 years) Coronary Artery Disease[J]. Biomedical and Environmental Sciences, 2016, 29(8): 545-554. doi: 10.3967/bes2016.073
Citation: DU Ying, YANG Sheng Hua, LI Sha, CUI ChuanJue, ZHANG Yan, ZHUChengGang, GUO YuanLin, WU NaQiong, GAO Ying, SUN Jing, DONG Qian, LIU Geng, LI JianJun. Circulating MicroRNAs as Novel Diagnostic Biomarkers for Very Early-onset (≤40 years) Coronary Artery Disease[J]. Biomedical and Environmental Sciences, 2016, 29(8): 545-554. doi: 10.3967/bes2016.073

Circulating MicroRNAs as Novel Diagnostic Biomarkers for Very Early-onset (≤40 years) Coronary Artery Disease

doi: 10.3967/bes2016.073
Funds:  This work was partially supported by the National Natural Science Foundation of China(81070171,81241121)%the Specialized Research Fund for the Doctoral Program of Higher Education of China(20111106110013)%the Capital Special Foundation of Clinical Application Research(Z121107001012015)%the Capital Health Development Fund(2011400302)%the Beijing Natural Science Foundation(7131014)
  • ObjectiveVery early-onset coronary artery disease (CAD) is a great challengein cardiovascular medicine throughout the world, especially regarding its early diagnosis. This study explored whether circulating microRNAs (miRNAs) could be used as potential biomarkers for patients with very early-onset CAD. MethodsWe performed an initial screening of miRNA expression using RNA isolated from 20 patients with angiographically documented very early-onset CAD and 20 age- and sex-matched normal controls. For further confirmation, we prospectively examined the miRNAs selected from 40 patientswithvery early-onset CAD and 40 angiography-normal controls. ResultsA total of 22 overexpressed miRNAs and 22 underexpressed miRNAs were detected in the initial screening. RT-qPCR analysisof the miRNAs obtained from the initial screening revealed that four miRNAs including miR-196-5p, miR-3163-3p, miR-145-3p, and miR-190a-5p exhibited significantly decreased expression in patients compared with that in controls (P<0.05).The areas under the receiver operating characteristic curve for these miRNAs were 0.824 (95% CI, 0.731-0.917;P<0.001), 0.758 (95%CI, 0.651-0.864;P<0.001), 0.753 (95% CI, 0.643-0.863;P<0.001), and 0.782 (95% CI, 0.680-0.884;P<0.001), respectively, in the validation set. ConclusionTo our knowledge, this isan advanced study to report about four serum miRNAs (miR-196-5p, miR-3163-3p, miR-145-3p, and miR-190a-5p) that could be used as novel biomarkers for the diagnosis of very early-onset CAD.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Circulating MicroRNAs as Novel Diagnostic Biomarkers for Very Early-onset (≤40 years) Coronary Artery Disease

doi: 10.3967/bes2016.073
Funds:  This work was partially supported by the National Natural Science Foundation of China(81070171,81241121)%the Specialized Research Fund for the Doctoral Program of Higher Education of China(20111106110013)%the Capital Special Foundation of Clinical Application Research(Z121107001012015)%the Capital Health Development Fund(2011400302)%the Beijing Natural Science Foundation(7131014)

Abstract: ObjectiveVery early-onset coronary artery disease (CAD) is a great challengein cardiovascular medicine throughout the world, especially regarding its early diagnosis. This study explored whether circulating microRNAs (miRNAs) could be used as potential biomarkers for patients with very early-onset CAD. MethodsWe performed an initial screening of miRNA expression using RNA isolated from 20 patients with angiographically documented very early-onset CAD and 20 age- and sex-matched normal controls. For further confirmation, we prospectively examined the miRNAs selected from 40 patientswithvery early-onset CAD and 40 angiography-normal controls. ResultsA total of 22 overexpressed miRNAs and 22 underexpressed miRNAs were detected in the initial screening. RT-qPCR analysisof the miRNAs obtained from the initial screening revealed that four miRNAs including miR-196-5p, miR-3163-3p, miR-145-3p, and miR-190a-5p exhibited significantly decreased expression in patients compared with that in controls (P<0.05).The areas under the receiver operating characteristic curve for these miRNAs were 0.824 (95% CI, 0.731-0.917;P<0.001), 0.758 (95%CI, 0.651-0.864;P<0.001), 0.753 (95% CI, 0.643-0.863;P<0.001), and 0.782 (95% CI, 0.680-0.884;P<0.001), respectively, in the validation set. ConclusionTo our knowledge, this isan advanced study to report about four serum miRNAs (miR-196-5p, miR-3163-3p, miR-145-3p, and miR-190a-5p) that could be used as novel biomarkers for the diagnosis of very early-onset CAD.

DU Ying, YANG Sheng Hua, LI Sha, CUI ChuanJue, ZHANG Yan, ZHUChengGang, GUO YuanLin, WU NaQiong, GAO Ying, SUN Jing, DONG Qian, LIU Geng, LI JianJun. Circulating MicroRNAs as Novel Diagnostic Biomarkers for Very Early-onset (≤40 years) Coronary Artery Disease[J]. Biomedical and Environmental Sciences, 2016, 29(8): 545-554. doi: 10.3967/bes2016.073
Citation: DU Ying, YANG Sheng Hua, LI Sha, CUI ChuanJue, ZHANG Yan, ZHUChengGang, GUO YuanLin, WU NaQiong, GAO Ying, SUN Jing, DONG Qian, LIU Geng, LI JianJun. Circulating MicroRNAs as Novel Diagnostic Biomarkers for Very Early-onset (≤40 years) Coronary Artery Disease[J]. Biomedical and Environmental Sciences, 2016, 29(8): 545-554. doi: 10.3967/bes2016.073

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