Studies of Chemical Constituents and Their Antioxidant Activities From Astragalus mongholicus Bunge
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Key words:
- Astragalus mongholicus Bunge /
- Constituents /
- Antioxidant /
- PC12 /
- Toxicity
Abstract: Objective To evaluate the antioxidant activities of different chemical constituents from Astragalus mongholicus Bunge and their protection against xanthine (XA)/xanthine oxidase (XO)-induced toxicity in PC12 cells. Methods The compounds of Astragalus mongholicus Bunge were isolated by chromatography and the structures were elucidated on the basis of spectral data interpretation. Their antioxidant activities were detected by 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities in a cell-free system. Meanwhile, the effects against XA/XO-induced toxicity were assessed using MTT assay in PC12 cells. Results Ten principal constituents were isolated and identified as formononetin (I), ononin (II), calycosin (III), calycosin-7-O-β-D-glucoside (IV), 9,10-dimethoxypterocarpan-3-O-β-D-glucoside (V), adenosine (VI), pinitol (VII), daucosterol (VIII), β-sitoster (IX) and saccharose (X) from Astragalus mongholicus Bunge. The compounds I, III, and IV scavenged DPPH free radicals in vitro. Formononetin and calycosin were found to inhibit XA/XO-induced cell injury significantly, with an estimated EC50 of 50 ng/mL. Conclusion Compound Ⅱ, Ⅵ, and Ⅶ are first reported in this plant. Calycosin exhibits the most potent antioxidant activity both in the cell-free system and in the cell system.
Citation: | DE-HONG YU, YONG-MING BAO, CHAO-LIANG WEI, LI-JIA AN. Studies of Chemical Constituents and Their Antioxidant Activities From Astragalus mongholicus Bunge[J]. Biomedical and Environmental Sciences, 2005, 18(5): 297-301. |