Tetracysteine as a Reporter for Gene Therapy

CHEN-YU XU; YING GU; WANG-HENG HOU; Yu-QIONG QUE; SHUANG-GUAN GAO; TONG CHENG; NING-SHAO XIA

PDF
Cite
Share

facebook

twitter

google

linkedin

Volume 22 Issue 6

Dec. 2009

Turn off MathJax

Article Contents

Article Navigation > Biomedical and Environmental Sciences > 2009 > 22(6): 496-501

CHEN-YU XU, YING GU, WANG-HENG HOU, Yu-QIONG QUE, SHUANG-GUAN GAO, TONG CHENG, NING-SHAO XIA. Tetracysteine as a Reporter for Gene Therapy[J]. Biomedical and Environmental Sciences, 2009, 22(6): 496-501.

Citation:

CHEN-YU XU, YING GU, WANG-HENG HOU, Yu-QIONG QUE, SHUANG-GUAN GAO, TONG CHENG, NING-SHAO XIA. Tetracysteine as a Reporter for Gene Therapy[J]. Biomedical and Environmental Sciences, 2009, 22(6): 496-501.

Tetracysteine as a Reporter for Gene Therapy

National Institute of Diagnostics and Vaccine Development for Infectious Diseases, School of Life Sciences, Xiamen University, Xiamen 361005, Fujian, China;Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005, Fujian, China
National Institute of Diagnostics and Vaccine Development for Infectious Diseases, School of Life Sciences, Xiamen University, Xiamen 361005, Fujian, China

Abstract

Objective To study the feasibility of using tetracysteine (TC) reporter in gene therapy. Methods Effects of TC reporter and conventional reporter genes encoding green fluorescence protein (GFP) and luciferase (Luc) on expression and function of the therapeutic gene MGMT~(P140K) were compared. Cytotoxicity and drug resistance were studied by Western blot. TC reporter used in therapy was analyzed by flow cytometry (FCM). Results The TC reporter had no toxicity to cells and neither affected the expression or activity of therapeutic gene as compared to GFP and Luc. TC could be used in blood sample detection. Conclusion TC is a new kind of reporter gene for lentiviral vector in future gene therapy.
- Tetracysteine,
- Lentiviral vector,
- Reporter,
- Gene therapy
References
Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article Metrics

Article views(936) PDF downloads(24) Cited by()

Proportional views

Tetracysteine as a Reporter for Gene Therapy

National Institute of Diagnostics and Vaccine Development for Infectious Diseases, School of Life Sciences, Xiamen University, Xiamen 361005, Fujian, China;Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005, Fujian, China

National Institute of Diagnostics and Vaccine Development for Infectious Diseases, School of Life Sciences, Xiamen University, Xiamen 361005, Fujian, China

Key words:

Abstract: Objective To study the feasibility of using tetracysteine (TC) reporter in gene therapy. Methods Effects of TC reporter and conventional reporter genes encoding green fluorescence protein (GFP) and luciferase (Luc) on expression and function of the therapeutic gene MGMT~(P140K) were compared. Cytotoxicity and drug resistance were studied by Western blot. TC reporter used in therapy was analyzed by flow cytometry (FCM). Results The TC reporter had no toxicity to cells and neither affected the expression or activity of therapeutic gene as compared to GFP and Luc. TC could be used in blood sample detection. Conclusion TC is a new kind of reporter gene for lentiviral vector in future gene therapy.

Citation: