Effects of the Extract of Ammopiptanthus mongolicus cheng f. (JA1) on Induction of Apoptosis of HepG2 in vitro and Its Molecular Mechanisms

LEI JIA; LI-ZHE AN

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Volume 19 Issue 2

Apr. 2006

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Article Navigation > Biomedical and Environmental Sciences > 2006 > 19(2): 118-123

LEI JIA, LI-ZHE AN. Effects of the Extract of Ammopiptanthus mongolicus cheng f. (JA1) on Induction of Apoptosis of HepG2 in vitro and Its Molecular Mechanisms[J]. Biomedical and Environmental Sciences, 2006, 19(2): 118-123.

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LEI JIA, LI-ZHE AN. Effects of the Extract of Ammopiptanthus mongolicus cheng f. (JA1) on Induction of Apoptosis of HepG2 in vitro and Its Molecular Mechanisms[J]. Biomedical and Environmental Sciences, 2006, 19(2): 118-123.

Effects of the Extract of Ammopiptanthus mongolicus cheng f. (JA1) on Induction of Apoptosis of HepG2 in vitro and Its Molecular Mechanisms

LEI JIA,
LI-ZHE AN

Key Laboratory of Arid and Pastoral Agroecology of Ministry of Education and School of Life Sciences, Lanzhou University, Lanzhou 730000, Gansu, China
Key Laboratory of Desert and Desertification, Cold and Arid Region Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, Gansu, China

Funds: National Key Technologies R&D Program of China(2001BA901A33)

Abstract

To study the effects and the mechanisms of extract from a leguminous plant (Ammopiptanthus mongolicus cheng f.) (JA1) in northwest China on inducing apoptosis and inhibiting proliferation of HepG2 hepatocarcinoma cell in vitro.Methods The HepG2 cell line was used as target cells. The effect of JA 1 on HepG2 cell growth was detected by microculture tetrazolium assay (MTT), flow cytometry assay, DNA agarose gel electrophoresis and transmission electronic microscopy. The expressive effect of the wt-p53 in HepG2 cells was analyzed with p53 protein test-reagent. Results JA1 not only had significant anti-proliferative effects depending upon time and dosage, but also induced apoptosis of HepG2 cells. Apoptotic typical morphological changes were observed in JA1-treated HepG2 cells under transmission electronic microscope, "Sub-Gl"phase peak occurred in flow cytometry and DNA "ladder" was found in DNA agarose gel electrophoresis. The expression of the wt-p53 increased in vitro, and JA1-treated HepG2 and the positive cell percentage of the wt-p53 protein also increased.Conclusions JA1 could obviously induce apoptosis and inhibit proliferation of HepG2 cells in vitro, and these effects are closely related with the increase of wt-p53 expression. JA1 can be used as a good source of medicinal plant for the treatment of hepatocarcinoma.
- Extract from Ammopiptanthus mongolicus cheng f. (JA 1),
- HepG2,
- Apoptosis,
- Induction,
- wt-p53
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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Effects of the Extract of Ammopiptanthus mongolicus cheng f. (JA1) on Induction of Apoptosis of HepG2 in vitro and Its Molecular Mechanisms

LEI JIA,
LI-ZHE AN

Key Laboratory of Arid and Pastoral Agroecology of Ministry of Education and School of Life Sciences, Lanzhou University, Lanzhou 730000, Gansu, China

Key Laboratory of Desert and Desertification, Cold and Arid Region Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, Gansu, China

Funds: National Key Technologies R&D Program of China(2001BA901A33)

Key words:

Extract from Ammopiptanthus mongolicus cheng f. (JA 1) /
HepG2 /
Apoptosis /
Induction /
wt-p53

Abstract: To study the effects and the mechanisms of extract from a leguminous plant (Ammopiptanthus mongolicus cheng f.) (JA1) in northwest China on inducing apoptosis and inhibiting proliferation of HepG2 hepatocarcinoma cell in vitro.Methods The HepG2 cell line was used as target cells. The effect of JA 1 on HepG2 cell growth was detected by microculture tetrazolium assay (MTT), flow cytometry assay, DNA agarose gel electrophoresis and transmission electronic microscopy. The expressive effect of the wt-p53 in HepG2 cells was analyzed with p53 protein test-reagent. Results JA1 not only had significant anti-proliferative effects depending upon time and dosage, but also induced apoptosis of HepG2 cells. Apoptotic typical morphological changes were observed in JA1-treated HepG2 cells under transmission electronic microscope, "Sub-Gl"phase peak occurred in flow cytometry and DNA "ladder" was found in DNA agarose gel electrophoresis. The expression of the wt-p53 increased in vitro, and JA1-treated HepG2 and the positive cell percentage of the wt-p53 protein also increased.Conclusions JA1 could obviously induce apoptosis and inhibit proliferation of HepG2 cells in vitro, and these effects are closely related with the increase of wt-p53 expression. JA1 can be used as a good source of medicinal plant for the treatment of hepatocarcinoma.

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