Volume 18 Issue 4
Aug.  2005
Turn off MathJax
Article Contents

XIN-LI XIAO, HUI-YING JIANG, JIN ZHANG, JUN HAN, KAI NIE, XIAO-BO ZHOU, YIN-XIA HUANG, LAN CHEN, WEI ZHOU, BAO-YUN ZHANG, YONG LIU, XIAO-PING DONG. Preparation of Monoclonal Antibodies Against Prion Proteins With Full-length Hamster PrP[J]. Biomedical and Environmental Sciences, 2005, 18(4): 273-280.
Citation: XIN-LI XIAO, HUI-YING JIANG, JIN ZHANG, JUN HAN, KAI NIE, XIAO-BO ZHOU, YIN-XIA HUANG, LAN CHEN, WEI ZHOU, BAO-YUN ZHANG, YONG LIU, XIAO-PING DONG. Preparation of Monoclonal Antibodies Against Prion Proteins With Full-length Hamster PrP[J]. Biomedical and Environmental Sciences, 2005, 18(4): 273-280.

Preparation of Monoclonal Antibodies Against Prion Proteins With Full-length Hamster PrP

Funds:  国家自然科学基金(30070038 and 30130070)%国家高技术研究发展计划(863计划)(2001AA215391)%EU Project QLRT(2000 01441)
  • Objective To prepare the PrP specific monoclonal antibodies (mAbs) that can be used for the detection of mammalian prions and study of pathogenesis of prion diseases. Methods Several BALB/c mice were immunized with recombinant hamster prion protein (HaPrP). Three hybridoma cell lines designated as B7, B9, and B10, secreting monoclonal antibodies against HaPrP, were established by hybridoma technique. The mAbs reactivities were evaluated with ELISA, Western blot, and immunohistochemistry. Results The mAbs produced by these cell lines reacted well with different recombinant hamster PrP proteins. Western blot analyses showed that mAbs B7 and B9 reacted with PrPSc from the scrapie-infected animals after proteinase K digestion with three glycosylated forms. The mAbs exhibited cross-reactivity with various PrPC from several other mammalian species, including humans and cattles. Immunohistochemistry assays confirmed that mAbs B7 and B9 could recognize not only extracellular but also intracellular PrPSc. Conclusion The mAbs of prion protein are successfully generated by hybridoma technique and can be applied for the diagnosis of prion associated diseases.
  • 加载中
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article Metrics

Article views(1215) PDF downloads(12) Cited by()

Proportional views
Related

Preparation of Monoclonal Antibodies Against Prion Proteins With Full-length Hamster PrP

Funds:  国家自然科学基金(30070038 and 30130070)%国家高技术研究发展计划(863计划)(2001AA215391)%EU Project QLRT(2000 01441)

Abstract: Objective To prepare the PrP specific monoclonal antibodies (mAbs) that can be used for the detection of mammalian prions and study of pathogenesis of prion diseases. Methods Several BALB/c mice were immunized with recombinant hamster prion protein (HaPrP). Three hybridoma cell lines designated as B7, B9, and B10, secreting monoclonal antibodies against HaPrP, were established by hybridoma technique. The mAbs reactivities were evaluated with ELISA, Western blot, and immunohistochemistry. Results The mAbs produced by these cell lines reacted well with different recombinant hamster PrP proteins. Western blot analyses showed that mAbs B7 and B9 reacted with PrPSc from the scrapie-infected animals after proteinase K digestion with three glycosylated forms. The mAbs exhibited cross-reactivity with various PrPC from several other mammalian species, including humans and cattles. Immunohistochemistry assays confirmed that mAbs B7 and B9 could recognize not only extracellular but also intracellular PrPSc. Conclusion The mAbs of prion protein are successfully generated by hybridoma technique and can be applied for the diagnosis of prion associated diseases.

XIN-LI XIAO, HUI-YING JIANG, JIN ZHANG, JUN HAN, KAI NIE, XIAO-BO ZHOU, YIN-XIA HUANG, LAN CHEN, WEI ZHOU, BAO-YUN ZHANG, YONG LIU, XIAO-PING DONG. Preparation of Monoclonal Antibodies Against Prion Proteins With Full-length Hamster PrP[J]. Biomedical and Environmental Sciences, 2005, 18(4): 273-280.
Citation: XIN-LI XIAO, HUI-YING JIANG, JIN ZHANG, JUN HAN, KAI NIE, XIAO-BO ZHOU, YIN-XIA HUANG, LAN CHEN, WEI ZHOU, BAO-YUN ZHANG, YONG LIU, XIAO-PING DONG. Preparation of Monoclonal Antibodies Against Prion Proteins With Full-length Hamster PrP[J]. Biomedical and Environmental Sciences, 2005, 18(4): 273-280.

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return