Oxidative Stress and Free Radical Damage in Patients With Acute Dipterex Poisoning

JUN-FU ZHOU; WEN ZHOU; SHU-MEI ZHANG; YAN-ER LUO; Huai-Hong Chen

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Volume 17 Issue 2

Jun. 2004

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Article Navigation > Biomedical and Environmental Sciences > 2004 > 17(2): 223-233

JUN-FU ZHOU, WEN ZHOU, SHU-MEI ZHANG, YAN-ER LUO, Huai-Hong Chen. Oxidative Stress and Free Radical Damage in Patients With Acute Dipterex Poisoning[J]. Biomedical and Environmental Sciences, 2004, 17(2): 223-233.

Citation:

JUN-FU ZHOU, WEN ZHOU, SHU-MEI ZHANG, YAN-ER LUO, Huai-Hong Chen. Oxidative Stress and Free Radical Damage in Patients With Acute Dipterex Poisoning[J]. Biomedical and Environmental Sciences, 2004, 17(2): 223-233.

Oxidative Stress and Free Radical Damage in Patients With Acute Dipterex Poisoning

Second Affiliated Hospital of College of Medicine, Zhejiang University, 88 Jiefang Road, Hangzhou 310009, Zhejiang, China;Zhejiang Surveillance Station of Drug Abuse, 88 Jiefang Road, Hangzhou 310009, Zhejiang, China
Second Affiliated Hospital of College of Medicine, Zhejiang University, 88 Jiefang Road, Hangzhou 310009, Zhejiang, China
Fuyang Municipal Hospital of Traditional Chinese Medicine, 4 Beimen Road, Fuyang 311400, Zhejiang, China

Abstract

Objective To investigate whether acute dipterex poisoning (ADP) may cause oxidative stress and free radical damage in the bodies of acute dipterex poisoning patients (ADPPs), and to explore the mechanisms by which ADP may cause oxidative stress and free radical damage. Methods Fifty ADPPs and fifty healthy adult volunteers (HAVs) whose ages, gender and others were matched with the ADPPs were enrolled in a randomized controlled study, in which concentrations of nitric oxide (NO), vitamin C (VC), vitamin E (VE) and (-carotene ((-CAR) in plasma as well as concentration of lipoperoxide (LPO), and activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and acetylcholinesterase (AChE) in erythrocytes were determined by spectrophotometric analytical methods. Results Compared with the average values of experimental parameters in the HAVs group, the average values of plasma NO and erythrocyte LPO in the ADPPs group were significantly increased (P<0.0001), while those of plasma VC, VE and β-CAR as well as erythrocyte SOD, CAT, GPX and AChE in the ADPPs group were significantly decreased (P<0.0001). Bivariate correlation analysis and partial correlation analysis suggested that when NO and LPO values were increased, and VC, VE, (-CAR, SOD, CAT and GPX values were decreased in the ADPPs, AChE value was decreased gradually in the ADPPs (P<0.001-0.0001). Reliability analysis of experimental parameters reflecting oxidative stress and free radical damage in the ADPPs showed that the reliability coefficient (8 items) alpha=0.6909, and the standardized item alpha=0.8574. Conclusion The findings in the present study suggest that ADP can cause oxidative stress and free radical damage, and inhibit markedly erythrocyte acetylcholinesterase activity in ADPPs.
- Dipterex,
- Dipterex poisoning,
- Oxidative stress,
- Free radical damage,
- Free radicals,
- Oxidation,
- Lipoperoxidation,
- Acetylcholinesterase
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Oxidative Stress and Free Radical Damage in Patients With Acute Dipterex Poisoning

Second Affiliated Hospital of College of Medicine, Zhejiang University, 88 Jiefang Road, Hangzhou 310009, Zhejiang, China;Zhejiang Surveillance Station of Drug Abuse, 88 Jiefang Road, Hangzhou 310009, Zhejiang, China

Second Affiliated Hospital of College of Medicine, Zhejiang University, 88 Jiefang Road, Hangzhou 310009, Zhejiang, China

Fuyang Municipal Hospital of Traditional Chinese Medicine, 4 Beimen Road, Fuyang 311400, Zhejiang, China

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Abstract: Objective To investigate whether acute dipterex poisoning (ADP) may cause oxidative stress and free radical damage in the bodies of acute dipterex poisoning patients (ADPPs), and to explore the mechanisms by which ADP may cause oxidative stress and free radical damage. Methods Fifty ADPPs and fifty healthy adult volunteers (HAVs) whose ages, gender and others were matched with the ADPPs were enrolled in a randomized controlled study, in which concentrations of nitric oxide (NO), vitamin C (VC), vitamin E (VE) and (-carotene ((-CAR) in plasma as well as concentration of lipoperoxide (LPO), and activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and acetylcholinesterase (AChE) in erythrocytes were determined by spectrophotometric analytical methods. Results Compared with the average values of experimental parameters in the HAVs group, the average values of plasma NO and erythrocyte LPO in the ADPPs group were significantly increased (P<0.0001), while those of plasma VC, VE and β-CAR as well as erythrocyte SOD, CAT, GPX and AChE in the ADPPs group were significantly decreased (P<0.0001). Bivariate correlation analysis and partial correlation analysis suggested that when NO and LPO values were increased, and VC, VE, (-CAR, SOD, CAT and GPX values were decreased in the ADPPs, AChE value was decreased gradually in the ADPPs (P<0.001-0.0001). Reliability analysis of experimental parameters reflecting oxidative stress and free radical damage in the ADPPs showed that the reliability coefficient (8 items) alpha=0.6909, and the standardized item alpha=0.8574. Conclusion The findings in the present study suggest that ADP can cause oxidative stress and free radical damage, and inhibit markedly erythrocyte acetylcholinesterase activity in ADPPs.

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