Schwann Cells Transplantation Promoted and the Repair of Brain Stem Injury in Rats

Hong WAN; YI-HUA AN; Mei-Zhen SUN; YA-ZHUO ZHANG; Zhong-Cheng WANG

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Volume 16 Issue 3

Sep. 2003

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Article Navigation > Biomedical and Environmental Sciences > 2003 > 16(3): 212-218

Hong WAN, YI-HUA AN, Mei-Zhen SUN, YA-ZHUO ZHANG, Zhong-Cheng WANG. Schwann Cells Transplantation Promoted and the Repair of Brain Stem Injury in Rats[J]. Biomedical and Environmental Sciences, 2003, 16(3): 212-218.

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Hong WAN, YI-HUA AN, Mei-Zhen SUN, YA-ZHUO ZHANG, Zhong-Cheng WANG. Schwann Cells Transplantation Promoted and the Repair of Brain Stem Injury in Rats[J]. Biomedical and Environmental Sciences, 2003, 16(3): 212-218.

Schwann Cells Transplantation Promoted and the Repair of Brain Stem Injury in Rats

Beijing Neurosurgical Institute, Beijing 100050, China

Funds: 国家自然科学基金(Grant 39970750)

Abstract

To explore the possibility of Schwann cells transplantation to promote the repair of injured brain stem reticular structure in rats. Methods Schwann cells originated from sciatic nerves of 1 to 2-day-old rats were expanded and labelled by BrdU in vitro, transplanted into rat brain stem reticular structure that was pre-injured by electric needle stimulus. Immunohistochemistry and myelin-staining were used to investigate the expression of BrdU, GAP-43 and new myelination respectively. Results BrdU positive cells could be identified for up to 8 months and their number increased by about 23%, which mainly migrated toward injured ipsilateral cortex. The GAP-43expression reached its peak in 1 month after transplantation and was significantly higher than that in the control group. New myelination could be seen in destructed brain stem areas. Conclusion The transplantation of Schwann cells can promote the restoration of injured brain stem reticular structure.
- Schwann cell,
- GAP-43,
- Brain stem
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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Schwann Cells Transplantation Promoted and the Repair of Brain Stem Injury in Rats

Beijing Neurosurgical Institute, Beijing 100050, China

Funds: 国家自然科学基金(Grant 39970750)

Key words:

Schwann cell /
GAP-43 /
Brain stem

Abstract: To explore the possibility of Schwann cells transplantation to promote the repair of injured brain stem reticular structure in rats. Methods Schwann cells originated from sciatic nerves of 1 to 2-day-old rats were expanded and labelled by BrdU in vitro, transplanted into rat brain stem reticular structure that was pre-injured by electric needle stimulus. Immunohistochemistry and myelin-staining were used to investigate the expression of BrdU, GAP-43 and new myelination respectively. Results BrdU positive cells could be identified for up to 8 months and their number increased by about 23%, which mainly migrated toward injured ipsilateral cortex. The GAP-43expression reached its peak in 1 month after transplantation and was significantly higher than that in the control group. New myelination could be seen in destructed brain stem areas. Conclusion The transplantation of Schwann cells can promote the restoration of injured brain stem reticular structure.

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