Volume 17 Issue 3
Sep.  2004
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XIAN-BING TANG, SHU-YI SI, YUE-QIN ZHANG. Identification of a New Peptide Deformylase Gene From Enterococcus faecium and Establishment of a New Screening Model Targeted on PDF for Novel Antibiotics[J]. Biomedical and Environmental Sciences, 2004, 17(3): 350-358.
Citation: XIAN-BING TANG, SHU-YI SI, YUE-QIN ZHANG. Identification of a New Peptide Deformylase Gene From Enterococcus faecium and Establishment of a New Screening Model Targeted on PDF for Novel Antibiotics[J]. Biomedical and Environmental Sciences, 2004, 17(3): 350-358.

Identification of a New Peptide Deformylase Gene From Enterococcus faecium and Establishment of a New Screening Model Targeted on PDF for Novel Antibiotics

Funds:  国家自然科学基金(grant 30270042)%Technology Platform of New Drugs Screen from Ministry of Science and Technology of China(2002AA22343D)
  • To identify a new peptide deformylase (PDF) gene (Genebank Accession AY238515) from Enterococcus faecium and to establish a new screening model targeted on PDF. Methods A new PDF gene was identified by BLAST analysis and PCR and was subsequently over-expressed in the prokaryotic expression host E.coli Bl21(DE3). Over-expressed protein was purified for enzymatic assay by metal affinity chromatography and a new screening model was established for novel antibiotics. Result A new PDF gene of Enterococcus faecium was identified successfully. Ten positive samples were picked up from 8000 compound library and the microbial fermentation broth samples. Conclusion A new PDF of gene Enterococcus faecium was first identified and the model had a high efficacy. Positive samples screened may be antibacterial agents of broad spectrum.
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通讯作者: 陈斌, bchen63@163.com
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Identification of a New Peptide Deformylase Gene From Enterococcus faecium and Establishment of a New Screening Model Targeted on PDF for Novel Antibiotics

Funds:  国家自然科学基金(grant 30270042)%Technology Platform of New Drugs Screen from Ministry of Science and Technology of China(2002AA22343D)

Abstract: To identify a new peptide deformylase (PDF) gene (Genebank Accession AY238515) from Enterococcus faecium and to establish a new screening model targeted on PDF. Methods A new PDF gene was identified by BLAST analysis and PCR and was subsequently over-expressed in the prokaryotic expression host E.coli Bl21(DE3). Over-expressed protein was purified for enzymatic assay by metal affinity chromatography and a new screening model was established for novel antibiotics. Result A new PDF gene of Enterococcus faecium was identified successfully. Ten positive samples were picked up from 8000 compound library and the microbial fermentation broth samples. Conclusion A new PDF of gene Enterococcus faecium was first identified and the model had a high efficacy. Positive samples screened may be antibacterial agents of broad spectrum.

XIAN-BING TANG, SHU-YI SI, YUE-QIN ZHANG. Identification of a New Peptide Deformylase Gene From Enterococcus faecium and Establishment of a New Screening Model Targeted on PDF for Novel Antibiotics[J]. Biomedical and Environmental Sciences, 2004, 17(3): 350-358.
Citation: XIAN-BING TANG, SHU-YI SI, YUE-QIN ZHANG. Identification of a New Peptide Deformylase Gene From Enterococcus faecium and Establishment of a New Screening Model Targeted on PDF for Novel Antibiotics[J]. Biomedical and Environmental Sciences, 2004, 17(3): 350-358.

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