Oxidative Damage to DNA and Its Relationship With Diabetic Complications

HONG-ZHI PAN; DONG CHANG; LEI-GUANG FENG; FENG-JUAN XU; HONG-YU KUANG; MING-JUN LU

PDF
Cite
Share

facebook

twitter

google

linkedin

Volume 20 Issue 2

Apr. 2007

Turn off MathJax

Article Contents

Article Navigation > Biomedical and Environmental Sciences > 2007 > 20(2): 160-163

HONG-ZHI PAN, DONG CHANG, LEI-GUANG FENG, FENG-JUAN XU, HONG-YU KUANG, MING-JUN LU. Oxidative Damage to DNA and Its Relationship With Diabetic Complications[J]. Biomedical and Environmental Sciences, 2007, 20(2): 160-163.

Citation:

HONG-ZHI PAN, DONG CHANG, LEI-GUANG FENG, FENG-JUAN XU, HONG-YU KUANG, MING-JUN LU. Oxidative Damage to DNA and Its Relationship With Diabetic Complications[J]. Biomedical and Environmental Sciences, 2007, 20(2): 160-163.

Oxidative Damage to DNA and Its Relationship With Diabetic Complications

School of Public Health, Harbin Medical University, Harbin 150081, Heilongjiang, China
The First Affiliated Hospital of the Harbin Medical University, Harbin 150001,Heilongjiang, China

Funds: Postgraduate Innovative Foundation of Harbin Medical University(HCXB2006008)%the Grant from Health Bureau of Heilongjiang Province(2005-91)

Abstract

Objective To detect the oxidative DNA damage in diabetic patients and to investigate the relationship of oxidative DNA damage with diabetes and diabetic nephropathy. Methods Single cell gel electrophoresis (SCGE) was used to detect the DNA strand breaks in peripheral blood lymphocytes, and oxidative DNA damage product and serum 8-OHdG were determined by a competitive ELISA in 47 cases, including 25 patients without diabetic complications, 22 patients with diabetic nephropathy and 25 normal control subjects. Results Diabetic patients showed greater oxidative damage to DNA. The percentage of comet cells and the length of DNA migration (comet tail length) of peripheral blood lymphocytes were significantly increased in patients with diabetes, and significantly higher in patients with diabetic nephropathy than in diabetic patients without vascular complications (P＜0.05). There was a significant increase in serum 8-OHdG in diabetic patients compared with normal subjects (P＜0.05). Moreover, serum 8-OHdG was much higher in patients with diabetic nephropathy than in diabetic patients without vascular complications (P＜0.05). Conclusion There is severe oxidative DNA damage in diabetic patients. Enhanced oxidative stress may be associated with diabetes, especially in patients with diabetic nephropathy.
- Diabetes,
- Diabetic nephropathy,
- Comet assay,
- 8-OHdG
References
Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article Metrics

Article views(1193) PDF downloads(54) Cited by()

Proportional views

Oxidative Damage to DNA and Its Relationship With Diabetic Complications

School of Public Health, Harbin Medical University, Harbin 150081, Heilongjiang, China

The First Affiliated Hospital of the Harbin Medical University, Harbin 150001,Heilongjiang, China

Funds: Postgraduate Innovative Foundation of Harbin Medical University(HCXB2006008)%the Grant from Health Bureau of Heilongjiang Province(2005-91)

Key words:

Abstract: Objective To detect the oxidative DNA damage in diabetic patients and to investigate the relationship of oxidative DNA damage with diabetes and diabetic nephropathy. Methods Single cell gel electrophoresis (SCGE) was used to detect the DNA strand breaks in peripheral blood lymphocytes, and oxidative DNA damage product and serum 8-OHdG were determined by a competitive ELISA in 47 cases, including 25 patients without diabetic complications, 22 patients with diabetic nephropathy and 25 normal control subjects. Results Diabetic patients showed greater oxidative damage to DNA. The percentage of comet cells and the length of DNA migration (comet tail length) of peripheral blood lymphocytes were significantly increased in patients with diabetes, and significantly higher in patients with diabetic nephropathy than in diabetic patients without vascular complications (P＜0.05). There was a significant increase in serum 8-OHdG in diabetic patients compared with normal subjects (P＜0.05). Moreover, serum 8-OHdG was much higher in patients with diabetic nephropathy than in diabetic patients without vascular complications (P＜0.05). Conclusion There is severe oxidative DNA damage in diabetic patients. Enhanced oxidative stress may be associated with diabetes, especially in patients with diabetic nephropathy.

Citation: