Volume 20 Issue 3
Jun.  2007
Turn off MathJax
Article Contents
Article Navigation >  Biomedical and Environmental Sciences  > 2007  >  20(3): 184-188

JIE-BO MI, JIN YAN, XIAO-JIE DING, ZHEN-QUAN GUO, MEI-PING ZHAO, WEN-BAO CHANG. Production and Characterization of Monoclonal Antibody Against Recombinant Human Erythropoietin[J]. Biomedical and Environmental Sciences, 2007, 20(3): 184-188.
Citation: JIE-BO MI, JIN YAN, XIAO-JIE DING, ZHEN-QUAN GUO, MEI-PING ZHAO, WEN-BAO CHANG. Production and Characterization of Monoclonal Antibody Against Recombinant Human Erythropoietin[J]. Biomedical and Environmental Sciences, 2007, 20(3): 184-188.
shu

Production and Characterization of Monoclonal Antibody Against Recombinant Human Erythropoietin

  • Beijing National Laboratory for Molecular Sciences(BNLMS),College of Chemistry and Molecular Engineering

  • College of Life Sciences,Peking University,Beijing 100871,China

Funds:  国家自然科学基金(20675006)
  • Objective To produce specific monoclonal antibody(mAb)against recombinant human erythropoietin(rHuEPO)for development of higmy efficient methods for erythropoietin detection in biological fluids.Methods rHuEPO was covalently coupled with bovine serum albumin(BSA)and the conjugate was used to immunize mice to produce specific mAb against rHuEPO based on hybridoma technology.The obtained F3-mAb was characterized by enzyme-linked immunosorbent assay (ELISA),SDS-PAGE and Western blot.Results The isotype of F3-mAb Was found to be IgM with an affinity constant of 2.1x108 L/mol.The competitive ELISA using the obtained IgM showed a broader linear range and lower detection limit compared with previous work.Conclusions The modification of rHuEPO was proved to be successful in generating required specific mAb with high avidity to rHuEPO.
  • 加载中
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索
Get Citation
PDF
XML

Article Metrics

Article views(1181) PDF downloads(18) Cited by()

Proportional views
Related

Production and Characterization of Monoclonal Antibody Against Recombinant Human Erythropoietin

  • Beijing National Laboratory for Molecular Sciences(BNLMS),College of Chemistry and Molecular Engineering
  • College of Life Sciences,Peking University,Beijing 100871,China
Funds:  国家自然科学基金(20675006)

Abstract: Objective To produce specific monoclonal antibody(mAb)against recombinant human erythropoietin(rHuEPO)for development of higmy efficient methods for erythropoietin detection in biological fluids.Methods rHuEPO was covalently coupled with bovine serum albumin(BSA)and the conjugate was used to immunize mice to produce specific mAb against rHuEPO based on hybridoma technology.The obtained F3-mAb was characterized by enzyme-linked immunosorbent assay (ELISA),SDS-PAGE and Western blot.Results The isotype of F3-mAb Was found to be IgM with an affinity constant of 2.1x108 L/mol.The competitive ELISA using the obtained IgM showed a broader linear range and lower detection limit compared with previous work.Conclusions The modification of rHuEPO was proved to be successful in generating required specific mAb with high avidity to rHuEPO.

JIE-BO MI, JIN YAN, XIAO-JIE DING, ZHEN-QUAN GUO, MEI-PING ZHAO, WEN-BAO CHANG. Production and Characterization of Monoclonal Antibody Against Recombinant Human Erythropoietin[J]. Biomedical and Environmental Sciences, 2007, 20(3): 184-188.
Citation: JIE-BO MI, JIN YAN, XIAO-JIE DING, ZHEN-QUAN GUO, MEI-PING ZHAO, WEN-BAO CHANG. Production and Characterization of Monoclonal Antibody Against Recombinant Human Erythropoietin[J]. Biomedical and Environmental Sciences, 2007, 20(3): 184-188.
shu

    HTML

Catalog

    Biomedical and Environmental Sciences ISSN 0895-3988 CN 11-2816/Q

    Publish Under the Auspices of Chinese Center for Disease Control and Prevention

    Distributed by Chinese Center for Disease Control and Prevention

    All Rights Reserved by Chinese Center for Disease Control and Prevention

    京ICP备11024750号-4     京公网京备11011402013006号

    Quick Links

    Supported by Beijing Renhe Information Technology Co. Ltd E-mail: info@rhhz.net 百度统计

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return