Volume 20 Issue 4
Aug.  2007
Turn off MathJax
Article Contents

FA-XIN HEI, YAN JIANG1, WEI-DONG SUN, QI-YUN ZHANG, QIN ZHANG, JING-RONG YE, HAI-LIN LIU, HONG-YAN LU, XIONG HE. Field Evaluation of Alternative Testing Strategies for the Detection of HIV Infection in Beijing[J]. Biomedical and Environmental Sciences, 2007, 20(4): 265-268.
Citation: FA-XIN HEI, YAN JIANG1, WEI-DONG SUN, QI-YUN ZHANG, QIN ZHANG, JING-RONG YE, HAI-LIN LIU, HONG-YAN LU, XIONG HE. Field Evaluation of Alternative Testing Strategies for the Detection of HIV Infection in Beijing[J]. Biomedical and Environmental Sciences, 2007, 20(4): 265-268.

Field Evaluation of Alternative Testing Strategies for the Detection of HIV Infection in Beijing

Funds:  As part of the AIDS Prevention Applied Research and Program of the Ministry of Health(WA2003-17)%the Department of Reference, National Center for AIDS Control and Prevention, Chinese Center for Disease Control and Prevention
  • Objective To identify a cost-efficient alternative antibody testing strategy for screening and confirmation of HIV infection by rapid simple tests (RSTs) and enzyme-linked immunosorbent assays (ELISAs). Methods Four RSTs (RST1,RST2, RST3, and RST4 ) and five ELISAs (ELISA1, ELISA2, ELISA3, ELISA4, and ELISA5) were evaluated in two phases by using banked and serum specimens prospectively collected at regional hospitals and voluntary counseling and testing (VCT)centers in Beijing. A total of 200 banked serum specimens were included in the first phase, including 62 HIV-positive, 127 HIV-negative and 11 indeterminate specimens. All specimens were tested by four RSTs and five ELISAs respectively. The second phase involved prospective testing of 389 routine specimens, including 92 HIV-positive, 287 HIV-negative, and 10 indeterminate specimens. All the specimens were tested by two RSTs (RST2 and RST4) and three ELISAs (ELISA1, ELISA3,and ELISA4), which were selected for their respective excellent sensitivity and/or specificity. Western blot (WB) was used as a gold standard for confirming the reactivity of all the specimens. Results Sensitivity, specificity, and efficacy were calculated for each assay in two phases. In the first phase, four assays (ELISA4, RST2, RST3, and RST4) had a specificity of 100%. For the determination of efficacy, ELISA4, RST2, and RST4 were selected in the second phase. ELISA1 and ELISA3 which have a sensitivity of 95.9% and 93.2% respectively also entered this phase. In the second phase, all the five assays (ELISA1, ELISA3, ELISA4, RST2, and RST4) had a sensitivity and specifity of over 90%. ELISA1 had a sensitivity of 99% and ELISA4 a specificity of 99%. Conclusion The sensitivity ELISA1 and the specificit of ELISA4 are comparable to ELISA/WB standard strategy. Application of this alternative testing strategy provides a cost-effective method for determining HIV prevalence in Beijing.
  • 加载中
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article Metrics

Article views(924) PDF downloads(12) Cited by()

Proportional views
Related

Field Evaluation of Alternative Testing Strategies for the Detection of HIV Infection in Beijing

Funds:  As part of the AIDS Prevention Applied Research and Program of the Ministry of Health(WA2003-17)%the Department of Reference, National Center for AIDS Control and Prevention, Chinese Center for Disease Control and Prevention

Abstract: Objective To identify a cost-efficient alternative antibody testing strategy for screening and confirmation of HIV infection by rapid simple tests (RSTs) and enzyme-linked immunosorbent assays (ELISAs). Methods Four RSTs (RST1,RST2, RST3, and RST4 ) and five ELISAs (ELISA1, ELISA2, ELISA3, ELISA4, and ELISA5) were evaluated in two phases by using banked and serum specimens prospectively collected at regional hospitals and voluntary counseling and testing (VCT)centers in Beijing. A total of 200 banked serum specimens were included in the first phase, including 62 HIV-positive, 127 HIV-negative and 11 indeterminate specimens. All specimens were tested by four RSTs and five ELISAs respectively. The second phase involved prospective testing of 389 routine specimens, including 92 HIV-positive, 287 HIV-negative, and 10 indeterminate specimens. All the specimens were tested by two RSTs (RST2 and RST4) and three ELISAs (ELISA1, ELISA3,and ELISA4), which were selected for their respective excellent sensitivity and/or specificity. Western blot (WB) was used as a gold standard for confirming the reactivity of all the specimens. Results Sensitivity, specificity, and efficacy were calculated for each assay in two phases. In the first phase, four assays (ELISA4, RST2, RST3, and RST4) had a specificity of 100%. For the determination of efficacy, ELISA4, RST2, and RST4 were selected in the second phase. ELISA1 and ELISA3 which have a sensitivity of 95.9% and 93.2% respectively also entered this phase. In the second phase, all the five assays (ELISA1, ELISA3, ELISA4, RST2, and RST4) had a sensitivity and specifity of over 90%. ELISA1 had a sensitivity of 99% and ELISA4 a specificity of 99%. Conclusion The sensitivity ELISA1 and the specificit of ELISA4 are comparable to ELISA/WB standard strategy. Application of this alternative testing strategy provides a cost-effective method for determining HIV prevalence in Beijing.

FA-XIN HEI, YAN JIANG1, WEI-DONG SUN, QI-YUN ZHANG, QIN ZHANG, JING-RONG YE, HAI-LIN LIU, HONG-YAN LU, XIONG HE. Field Evaluation of Alternative Testing Strategies for the Detection of HIV Infection in Beijing[J]. Biomedical and Environmental Sciences, 2007, 20(4): 265-268.
Citation: FA-XIN HEI, YAN JIANG1, WEI-DONG SUN, QI-YUN ZHANG, QIN ZHANG, JING-RONG YE, HAI-LIN LIU, HONG-YAN LU, XIONG HE. Field Evaluation of Alternative Testing Strategies for the Detection of HIV Infection in Beijing[J]. Biomedical and Environmental Sciences, 2007, 20(4): 265-268.

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return