Volume 17 Issue 2
Jun.  2004
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YA-JUAN GAO, CHENG-FENG XIAO, Sheng CHEN, Rui-Bo Wang, HAN-ZHEN HE, ROBERT M TANGUAY, Tang-Chun Wu. In vitro Study on Role of Hsp70 Expression in DNA Damage of Human Embryonic Lung Cells Exposed to Benzo[a]pyrene[J]. Biomedical and Environmental Sciences, 2004, 17(2): 144-152.
Citation: YA-JUAN GAO, CHENG-FENG XIAO, Sheng CHEN, Rui-Bo Wang, HAN-ZHEN HE, ROBERT M TANGUAY, Tang-Chun Wu. In vitro Study on Role of Hsp70 Expression in DNA Damage of Human Embryonic Lung Cells Exposed to Benzo[a]pyrene[J]. Biomedical and Environmental Sciences, 2004, 17(2): 144-152.

In vitro Study on Role of Hsp70 Expression in DNA Damage of Human Embryonic Lung Cells Exposed to Benzo[a]pyrene

Funds:  This work was supported by grants from the National Natural Science Foundation of China (NNSFC)%国家重点基础研究发展计划(973计划)(2002 CB512905)%国家自然科学基金
  • Objective Benzo[a]pyrene (B[a]P), a ubiquitous environmental pollutant, is a potent procarcinogen and mutagen that can elicit tumors, leading to malignancy. Heat shock proteins (Hsp) have been shown to protect cells against damages caused by various stresses including exposure to numerous chemicals. Whether Hsps, or more specifically Hsp70, are involved in repair of B[a]P-induced DNA damage is currently unknown. Methods We assessed the potential role of the inducible form of Hsp70 in B[a]P-induced DNA damage of human embryonic lung (HEL) cells using immunoblot and the comet assay (i.e., the single cell gel electrophoresis assay). Results Exposure to B[a]P induced a dose-dependent decrease in the level of Hsp70, but a dose-dependent increase in DNA damage both in untreated (control) HEL cells and in cells preconditioned by a heat treatment. Heat preconditioning prior to B[a]P exposure potentiated the effect of B[a]P at a low dose (10 (mol/L), but appeared to be protective at higher doses. There was a negative correlation between Hsp70 level and DNA damage in the non-preheated as well as in the preconditioned cells. Conclusion These data suggest that exposure of HEL cells to B[a]P may induce a dose-dependent reduction in the levels of the inducible Hsp70. The detailed mechanisms for the reduction of Hsp70 levels by B[a]P and the role of Hsp70 in DNA damage under different concentrations of B[a]P remains to be determined.
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In vitro Study on Role of Hsp70 Expression in DNA Damage of Human Embryonic Lung Cells Exposed to Benzo[a]pyrene

Funds:  This work was supported by grants from the National Natural Science Foundation of China (NNSFC)%国家重点基础研究发展计划(973计划)(2002 CB512905)%国家自然科学基金

Abstract: Objective Benzo[a]pyrene (B[a]P), a ubiquitous environmental pollutant, is a potent procarcinogen and mutagen that can elicit tumors, leading to malignancy. Heat shock proteins (Hsp) have been shown to protect cells against damages caused by various stresses including exposure to numerous chemicals. Whether Hsps, or more specifically Hsp70, are involved in repair of B[a]P-induced DNA damage is currently unknown. Methods We assessed the potential role of the inducible form of Hsp70 in B[a]P-induced DNA damage of human embryonic lung (HEL) cells using immunoblot and the comet assay (i.e., the single cell gel electrophoresis assay). Results Exposure to B[a]P induced a dose-dependent decrease in the level of Hsp70, but a dose-dependent increase in DNA damage both in untreated (control) HEL cells and in cells preconditioned by a heat treatment. Heat preconditioning prior to B[a]P exposure potentiated the effect of B[a]P at a low dose (10 (mol/L), but appeared to be protective at higher doses. There was a negative correlation between Hsp70 level and DNA damage in the non-preheated as well as in the preconditioned cells. Conclusion These data suggest that exposure of HEL cells to B[a]P may induce a dose-dependent reduction in the levels of the inducible Hsp70. The detailed mechanisms for the reduction of Hsp70 levels by B[a]P and the role of Hsp70 in DNA damage under different concentrations of B[a]P remains to be determined.

YA-JUAN GAO, CHENG-FENG XIAO, Sheng CHEN, Rui-Bo Wang, HAN-ZHEN HE, ROBERT M TANGUAY, Tang-Chun Wu. In vitro Study on Role of Hsp70 Expression in DNA Damage of Human Embryonic Lung Cells Exposed to Benzo[a]pyrene[J]. Biomedical and Environmental Sciences, 2004, 17(2): 144-152.
Citation: YA-JUAN GAO, CHENG-FENG XIAO, Sheng CHEN, Rui-Bo Wang, HAN-ZHEN HE, ROBERT M TANGUAY, Tang-Chun Wu. In vitro Study on Role of Hsp70 Expression in DNA Damage of Human Embryonic Lung Cells Exposed to Benzo[a]pyrene[J]. Biomedical and Environmental Sciences, 2004, 17(2): 144-152.

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