Application of PCR-DGGE in Research of Bacterial Diversity in Drinking Water

QING WU; XIN-HUA ZHAO; SHENG-YUE ZHAO

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Volume 19 Issue 5

Oct. 2006

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Article Navigation > Biomedical and Environmental Sciences > 2006 > 19(5): 371-374

QING WU, XIN-HUA ZHAO, SHENG-YUE ZHAO. Application of PCR-DGGE in Research of Bacterial Diversity in Drinking Water[J]. Biomedical and Environmental Sciences, 2006, 19(5): 371-374.

Citation:

QING WU, XIN-HUA ZHAO, SHENG-YUE ZHAO. Application of PCR-DGGE in Research of Bacterial Diversity in Drinking Water[J]. Biomedical and Environmental Sciences, 2006, 19(5): 371-374.

Application of PCR-DGGE in Research of Bacterial Diversity in Drinking Water

Department of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China

Funds: 国家自然科学基金(50478086)%The 10th Five-year Key Programs for Science and Technology Development of China(2002AA601120)

Abstract

Objective To analyze the structure of bacteria in drinking water by molecular biological techniques. Methods DNA of bacteria in drinking water was directly extracted without culture. 16S ribosomal DNA fragments, including V-6, -7, and -8regions, were amplified with universal primers (EUBf933GC and EUBr1387) and analyzed by DGGE. Results DGGE indicated that amplification products could be separated. The results showed that DGGE could be used in the separation of different microbial 16SrRNA genes extracted from drinking water. Though there were special bacteria in different water samples, the predominant bacteria were essentially the same. Three sequences of the reclaimed specific bands were obtained, and phylogenetic tree of these bands was made. Conclusion Bacterial diversity in drinking water is identified by molecular biological techniques.
- Drinking water,
- Denaturing gradient gel electrophoresis (DGGE),
- 16S ribosome RNA,
- Microbial diversity
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Application of PCR-DGGE in Research of Bacterial Diversity in Drinking Water

Department of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China

Funds: 国家自然科学基金(50478086)%The 10th Five-year Key Programs for Science and Technology Development of China(2002AA601120)

Key words:

Drinking water /
Denaturing gradient gel electrophoresis (DGGE) /
16S ribosome RNA /
Microbial diversity

Abstract: Objective To analyze the structure of bacteria in drinking water by molecular biological techniques. Methods DNA of bacteria in drinking water was directly extracted without culture. 16S ribosomal DNA fragments, including V-6, -7, and -8regions, were amplified with universal primers (EUBf933GC and EUBr1387) and analyzed by DGGE. Results DGGE indicated that amplification products could be separated. The results showed that DGGE could be used in the separation of different microbial 16SrRNA genes extracted from drinking water. Though there were special bacteria in different water samples, the predominant bacteria were essentially the same. Three sequences of the reclaimed specific bands were obtained, and phylogenetic tree of these bands was made. Conclusion Bacterial diversity in drinking water is identified by molecular biological techniques.

QING WU, XIN-HUA ZHAO, SHENG-YUE ZHAO. Application of PCR-DGGE in Research of Bacterial Diversity in Drinking Water[J]. Biomedical and Environmental Sciences, 2006, 19(5): 371-374.

Citation:

QING WU, XIN-HUA ZHAO, SHENG-YUE ZHAO. Application of PCR-DGGE in Research of Bacterial Diversity in Drinking Water[J]. Biomedical and Environmental Sciences, 2006, 19(5): 371-374.