Induction of Apoptosis by Superoxide Anion and the Protective Effects of Selenium and Vitamin E

GUO LING; XUE AN-NA; WANG Shu-qin; CHEN Jing-yan; WU YUAN-DE; ZHANG BIN

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Volume 14 Issue 3

Sep. 2001

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Article Navigation > Biomedical and Environmental Sciences > 2001 > 14(3): 241-247

GUO LING, XUE AN-NA, WANG Shu-qin, CHEN Jing-yan, WU YUAN-DE, ZHANG BIN. Induction of Apoptosis by Superoxide Anion and the Protective Effects of Selenium and Vitamin E[J]. Biomedical and Environmental Sciences, 2001, 14(3): 241-247.

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GUO LING, XUE AN-NA, WANG Shu-qin, CHEN Jing-yan, WU YUAN-DE, ZHANG BIN. Induction of Apoptosis by Superoxide Anion and the Protective Effects of Selenium and Vitamin E[J]. Biomedical and Environmental Sciences, 2001, 14(3): 241-247.

Induction of Apoptosis by Superoxide Anion and the Protective Effects of Selenium and Vitamin E

Institute of Nutrition and Food Hygiene, Chinese Academy of Preventive Medicine, Nan Wei Road 29,

Abstract

Objective The purpose of this study is to investigate the effect of superoxide anion on the apoptosis of cultured fibroblasts and the protective role of selenium and Vitamin E. Methods Cultured fibroblasts (NIH3T3), with or without selenium or vitamin E in the medium, were treated by superoxide anion produced by xanthine/xanthine oxidase reaction system and changes in cell structure and DNA were observed microscopically and electrophoretically. Results Apoptosis was observed when superoxide anion at a concentration of 5 nmol/L or 10 nmol/L had acted on the fibroblasts for 5-10 h. Selenium and Vitamin E in the medium inhibited the apoptosis significantly when their concentrations reached 1.15 mol/L and 2.3 mol/L respectively. Conclusion　Selenium and vitamin E have protective effect against the apoptosis induced by superoxide anion. The effect of selenium is more remarkable than that of vitamin E.
- Superoxide Anion,
- superoxide anion,
- Vitamin E,
- protective effect,
- cell structure
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Induction of Apoptosis by Superoxide Anion and the Protective Effects of Selenium and Vitamin E

Institute of Nutrition and Food Hygiene, Chinese Academy of Preventive Medicine, Nan Wei Road 29,

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Abstract: Objective The purpose of this study is to investigate the effect of superoxide anion on the apoptosis of cultured fibroblasts and the protective role of selenium and Vitamin E. Methods Cultured fibroblasts (NIH3T3), with or without selenium or vitamin E in the medium, were treated by superoxide anion produced by xanthine/xanthine oxidase reaction system and changes in cell structure and DNA were observed microscopically and electrophoretically. Results Apoptosis was observed when superoxide anion at a concentration of 5 nmol/L or 10 nmol/L had acted on the fibroblasts for 5-10 h. Selenium and Vitamin E in the medium inhibited the apoptosis significantly when their concentrations reached 1.15 mol/L and 2.3 mol/L respectively. Conclusion　Selenium and vitamin E have protective effect against the apoptosis induced by superoxide anion. The effect of selenium is more remarkable than that of vitamin E.

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