Isolation and Characterization of Nickel Uptake by Nickel Resistant Bacterial Isolate (NiRBI)

JAGDISH S PATEL; PRERNA C PATEL; KIRAN KALIA

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Volume 19 Issue 4

Aug. 2006

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Article Navigation > Biomedical and Environmental Sciences > 2006 > 19(4): 297-301

JAGDISH S PATEL, PRERNA C PATEL, KIRAN KALIA. Isolation and Characterization of Nickel Uptake by Nickel Resistant Bacterial Isolate (NiRBI)[J]. Biomedical and Environmental Sciences, 2006, 19(4): 297-301.

Citation:

JAGDISH S PATEL, PRERNA C PATEL, KIRAN KALIA. Isolation and Characterization of Nickel Uptake by Nickel Resistant Bacterial Isolate (NiRBI)[J]. Biomedical and Environmental Sciences, 2006, 19(4): 297-301.

Isolation and Characterization of Nickel Uptake by Nickel Resistant Bacterial Isolate (NiRBI)

Department of Biosciences, Sardar Patel University, Vallabh Vidyanagar-388 120, Gujarat, India

Abstract

Bioremediation technology has gained importance because microbes could be the convenient source of bio-absorption/bioaccumulation of metals from effluent streams. Methods The nickel-resistant bacterial isolates (NiRBI)were selected from various bacterial isolates from industrial effluent and grown in nutrient broth containing different concentrations of nickel sulfate (0.3-3.0 mmol/L) and their capability of accumulating metal from the medium. Results Well-defined growth of NiRBI was observed in the medium containing up to 2.5 mmol/L of nickel. The isolate was identified using 16S rRNA and closely related to Pseudomonas fragi. Maximum accumulation of nickel (0.59 mg/g dry weight of bacterial cells) was observed when NiRBI was grown in media containing 2 mmol/L of nickel. The protein profile of the NiRBI cellular extract by SDS-PAGE showed two metal stress-induced proteins of molecular weight 48 KD and 18 KD with a simultaneous down regulation of four proteins of 46.7 KD, 42.2 KD, 19.7 KD, and 4.0 KD. Conclusion 48 KD and 18 KD proteins play a role in metal resistance mechanism by NiRBI.
- Nickel,
- Nickel-resistant bacterial isolates,
- Induction,
- Repression,
- Proteins
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Isolation and Characterization of Nickel Uptake by Nickel Resistant Bacterial Isolate (NiRBI)

Department of Biosciences, Sardar Patel University, Vallabh Vidyanagar-388 120, Gujarat, India

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Abstract: Bioremediation technology has gained importance because microbes could be the convenient source of bio-absorption/bioaccumulation of metals from effluent streams. Methods The nickel-resistant bacterial isolates (NiRBI)were selected from various bacterial isolates from industrial effluent and grown in nutrient broth containing different concentrations of nickel sulfate (0.3-3.0 mmol/L) and their capability of accumulating metal from the medium. Results Well-defined growth of NiRBI was observed in the medium containing up to 2.5 mmol/L of nickel. The isolate was identified using 16S rRNA and closely related to Pseudomonas fragi. Maximum accumulation of nickel (0.59 mg/g dry weight of bacterial cells) was observed when NiRBI was grown in media containing 2 mmol/L of nickel. The protein profile of the NiRBI cellular extract by SDS-PAGE showed two metal stress-induced proteins of molecular weight 48 KD and 18 KD with a simultaneous down regulation of four proteins of 46.7 KD, 42.2 KD, 19.7 KD, and 4.0 KD. Conclusion 48 KD and 18 KD proteins play a role in metal resistance mechanism by NiRBI.

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