Articles in press have been peer-reviewed and accepted, which are not yet assigned to volumes /issues, but are citable by Digital Object Identifier (DOI).
Coronavirus Disease 2019 versus Influenza A in Children: An Observational Control Study in China
ZHAO Yang, SUN De Lin, Heather C. Bouchard, ZHANG Xin Xin, WAN Gang, HAO Yi Wei, HE Shu Xin, JIANG Yu Yong, PANG Lin
Corrected proof  doi: 10.3967/bes2020.080
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This study aimed to understand the differences in clinical, epidemiological, and laboratory features between the new coronavirus disease 2019 (COVID-2019) and influenza A in children.Data of 23 hospitalized children with COVID-19 (9 boys, 5.7 ± 3.8 years old) were compared with age- and sex-matched 69 hospitalized and 69 outpatient children with influenza A from a hospital in China. The participants’ epidemiological history, family cluster, clinical manifestations, and blood test results were assessed.Compared with either inpatients or outpatients with influenza A, children with COVID-19 showed significantly more frequent family infections and higher ratio of low fever (< 37.3 °C), but shorter cough and fever duration, lower body temperature, and lower rates of cough, fever, high fever (> 39 °C), nasal congestion, rhinorrhea, sore throat, vomiting, myalgia or arthralgia, and febrile seizures. They also showed higher counts of lymphocytes, T lymphocyte CD8, and platelets and levels of cholinesterase, aspartate aminotransferase, lactate dehydrogenase, and lactic acid, but lower serum amyloid, C-reactive protein, and fibrinogen levels and erythrocyte sedimentation rate, and shorter prothrombin time. The level of alanine aminotransferase in children with COVID-19 is lower than that in inpatients but higher than that in outpatients with influenza A.Pediatric COVID-19 is associated with more frequent family infection, milder symptoms, and milder immune responses relative to pediatric influenza A.
Assessment of the Sensitivity and Specificity of the Established Real-time Quaking-induced Conversion (RT-QuIC) Technique in Chinese CJD Surveillance
XIAO Kang, YANG Xue Hua, ZOU Wen Quan, DONG Xiao Ping, SHI Qi
Corrected proof  doi: 10.3967/bes2020.081
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Real-time quaking-induced conversion (RT-QuIC) assay is a newly established PrPSc-detecting method. The development of RT-QuIC improves the diagnosis of sporadic Creutzfeldt–Jakob disease (sCJD), showing good sensitivity and specificity in many countries when the method was used in cerebrospinal fluid (CSF) samples. However, in China, the sensitivity and specificity of RT-QuIC has yet to be determined due to the lack of definitive diagnosis samples. Recently, 30 definitive sCJD and 30 non-CJD diagnoses were evaluated by RT-QuIC assay. In the 30 sCJD CSF samples, 29 showed positive results. By contrast, all the non-CJD samples were negative. The sensitivity and specificity of our RT-QuIC assay were 96.67% and 100%, respectively, and are comparable to other published data. Results can provide a fundamental basis for the usage of RT-QuIC assay in CJD surveillance in China.
Basal Energy Expenditure of Chinese Healthy Adults: Comparison of Measured and Predicted Values
MAO De Qian, WU Jing Huan, HUANG Cheng Yu, LI Ke Ji, LIU Xiao Li, ZHANG Shi Lian, WANG Yan Ling, CHEN Wei, LI Ming, YANG Xiao Guang, PIAO Jian Hua
Corrected proof  doi: 10.3967/bes2020.075
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  Objective  This study aimed to measure the basal energy expenditure (BEE) of Chinese healthy adults and establish an accurate predictive equation for this population.  Methods  In total, 470 Chinese healthy adults had their BEE measured using the Cosmed K4b2 portable metabolic system. Multiple linear regression analysis was applied to develop new optimal equations for predicting BEE. The bias, accuracy rate, concordance correlation coefficient (CCC), and root mean square error (RMSE) were used to evaluate the accuracy of the predictive equations.  Results  There was a significant difference in BEE between males and females, with 5,954 kJ/d and 5,089 kJ/d, respectively. People living in rural areas expended significantly higher BEE (5,885 kJ/d) than those in urban areas (5,279 kJ/d). Previous equations developed by Henry, Schofield, Harris-Benedict (H-B), and Liu overestimated the BEE of Chinese healthy adults. The new equations derived from the present study displayed the smallest average bias and RMSE from the measured basal energy expenditure (mBEE). The CCC of the new equations was higher than other predictive equations, but it was lower than 0.8. There was no significant difference in the accuracy rate among all predictive equations.  Conclusions  Sex and regional differences in BEE were observed in Chinese healthy adults. Neither the widely used previous predictive equations nor the one derived in the present study were accurate enough for estimating the BEE of Chinese healthy adults. Further study is required to develop more accurate equations for predicting the BEE of Chinese healthy adults aged between 20–45 years.
2020, 33(7): 0-0.  
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2020, 33(7): 1-2.  
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Original Article
Photothermal Effect-based Cytotoxic Ability of Melanin from Mytilus edulis Shells to Heal Wounds Infected with Drug-resistant Bacteria in vivo
LIU Ya Mei, MA Wei Shuai, WEI Yu Xi, XU Yuan Hong
2020, 33(7): 471-483.   doi: 10.3967/bes2020.052
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  Objective  Owing to antibiotic abuse and the subsequent development of antibiotic resistance, bacterial infection has become one of the most persistent unresolved problems. New antibacterial agents, especially those that are environmental-friendly, are urgently needed.  Methods  Melanin extracted by filtration centrifugation and acid and proteolytic hydrolysis was characterized using UV, FTIR, TEM, and XPS. Photothermal conversion was calculated, and the bacteriostatic effects, in vitro and in vivo, were assessed by plate counting and ratios (%) of wound areas.  Results  Natural melanin hydrolyzed by trypsin had good photothermal conversion effects, which resulted in superior bacteriostatic activities. The extracted melanin along with laser NIR irradiation at 808 nm promoted the healing of wounds infected by drug-resistant bacteria in vivo and was biocompatible according to toxicity tests in vivo and in vitro.  Conclusion  The present findings indicated a safe and efficient method of developing natural antibacterial agents.
Modeling the Long-term Antibody Response and Duration of Immune Protection Induced by an Inactivated, Preservative-free Hepatitis A Vaccine (Healive) in Children
YU Yong Pei, CHEN Jiang Ting, JIANG Zhi Wei, WANG Ling, YU Cheng Kai, YAN Xiao Yan, YAO Chen, XIA Jie Lai
2020, 33(7): 484-492.   doi: 10.3967/bes2020.065
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  Objective  Long-term seroprotection via the hepatitis A vaccine is essential for the prevention of disease from the hepatitis A virus (HAV). Due to documented difficulties during decade-long follow-ups after receiving vaccines, statistical-modeling approaches have been applied to predict the duration of immune protection.  Methods  Based on five-year follow-up data from a randomized positive-controlled trial among Chinese children (1–8 years old) following a 0, 6 months vaccination schedule, a power-law model accounting for the kinetics of B-cell turnover, as well as a modified power-law model considering a memory-B-cell subpopulation, were fitted to predict the long-term immune responses induced by HAV vaccination (Healive or Havrix). Anti-HAV levels of each individual and seroconversion rates up to 30 years after vaccination were predicted.  Results  A total of 375 participants who completed the two-dose vaccination were included in the analysis. Both models predicted that, over a life-long period, participants vaccinated with Healive would have close but slightly higher antibody titers than those of participants vaccinated with Havrix. Additionally, consistent with previous studies, more than 90% of participants were predicted to maintain seroconversion for at least 30 years. Moreover, the modified power-law model predicted that the antibody titers would reach a plateau level after nearly 15 years post-vaccination.  Conclusions  Based on the results of our modeling, Healive may adequately induce long-term immune responses following a 0, 6 months vaccination schedule in children via induction of memory B cells to provide stable and durable immune protection.
Preparation of PrP-specific Polyclonal Antibody via Immunization of PRNP-knockout Mice with Recombinant Human PrP Protein
YANG Xue Hua, WU Yue Zhang, XIAO Kang, GAO Li Ping, CHEN Dong Dong, DONG Xiao Ping, SHI Qi
2020, 33(7): 493-501.   doi: 10.3967/bes2020.066
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  Objective  The definite diagnosis of human and animal prion diseases depends on the examination of special pathological changes and/or detection of PrPSc in the brain tissues of suspected cases. Thus, developing methods to obtain PrP antibody with good specificity and sensitivity is fundamental for prion identification.  Methods  We prepared a PrP-specific polyclonal antibody (pAb P54) in a PRNP-knockout mouse model via immunization with recombinant full-length human PrP protein residues 23–231. Thereafter, we verified that pAb in Western blot, immunohistochemistry (IHC), and immunofluorescent (IFA) assays.  Results  Western blot illustrated that the newly prepared pAb P54 could react with recombinant PrP protein, normal brain PrPC from healthy rodents and humans, and pathological PrPSc in the brains of experimental rodents infected with scrapie and humans infected with different types of prion diseases. The electrophoretic patterns of brain PrPC and PrPSc observed after their reaction with pAb P54 were nearly identical to those produced by commercial PrP monoclonal antibodies. Three glycosylated PrP molecules in the brain homogenates were clearly demonstrated in the reactions of these molecules with pAb P54. IHC assay revealed apparent PrP deposits in the GdnCl-treated brain slices of 139A-infected mice and 263K-infected hamsters. IFA tests with pAb P54 also showed clear green signals surrounding blue-stained cell nuclei.  Conclusion  The newly prepared pAb P54 demonstrated reliable specificity and sensitivity and, thus, may have potential applications not only in studies of prion biology but also in the diagnosis of human and experimental rodent prion diseases.
Analysis of Factors Influencing Insomnia and Construction of a Prediction Model: A Cross-sectional Survey on Rescuers
SAI Xiao Yong, CHEN Qiao, LUO Ting Gang, SUN Yuan Yuan, SONG Yu Jian, CHEN Juan
2020, 33(7): 502-509.   doi: 10.3967/bes2020.067
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  Objective  To determine the factors influencing insomnia and construct early insomnia warning tools for rescuers to informbest practices for early screening and intervention.  Methods  Cluster sampling was used to conduct a cross-sectional survey of 1,133 rescuers from one unit in Beijing, China. Logistic regression modeling and R software were used to analyze insomnia-related factors and construct a PRISM model, respectively.  Results  The positive rate of insomnia among rescuers was 2.74%. Accounting for participants’ age, education, systolic pressure, smoking, per capita family monthly income, psychological resilience, and cognitive emotion regulation, logistic regression analysis revealed that, compared with families with an average monthly income less than 3,000 yuan, the odds ratio (OR) values and the [95% confidence interval (CI)] for participants of the following categories were as follows: average monthly family income greater than 5,000 yuan: 2.998 (1.307–6.879), smoking: 4.124 (1.954–8.706), and psychological resilience: 0.960 (0.933–0.988). The ROC curve area of the PRISM model (AUC) = 0.7650, specificity = 0.7169, and sensitivity = 0.7419.  Conclusion  Insomnia was related to the participants’ per capita family monthly income, smoking habits, and psychological resilience on rescue workers. The PRISM model’s good diagnostic value advises its use to screen rescuer early sleep quality. Further, advisable interventions to optimize sleep quality and battle effectiveness include psychological resilience training and smoking cessation.
Free Flow-through Anterolateral Thigh Flaps for Wrist High-tension Electrical Burns: A Retrospective Case Series
WANG Cheng, SHEN Yu Ming, QIN Feng Jun, HU Xiao Hua
2020, 33(7): 510-517.   doi: 10.3967/bes2020.064
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  Objective  The objective of this report was to demonstrate the clinical application of free flow-through anterolateral thigh flaps for the treatment of high-tension electrical wrist burns.  Methods  We collected the data of 8 patients with high-tension electrical wrist burns admitted to Beijing Jishuitan Hospital from January 2014 to December 2018. The clinical and pathological data were extracted from electronic hospital medical records. We obtained follow-up information through clinic visits.  Results  The injury sites for all 8 patients were the wrists, specifically 5 right and 3 left wrists, all of which were on the flexor side. Five patients had ulnar artery embolism necrosis and patency, with injury to the radial artery. Two patients had ulnar and radial arterial embolization and necrosis. The last patient had ulnar arterial embolization and necrosis with a normal radial artery. After debridement, the wound area ranged from 12 cm × 9 cm to 25 cm × 16 cm. The diagnoses for the eight patients were type II to type III high-tension electrical wrist burns. Free flow-through anterolateral thigh flaps (combined with great saphenous vein transplantation if necessary) were used to repair the wounds. The prognosis for all patients was good after six months to one year of follow-up.  Conclusion  Treating wrist types II and III high-tension electrical burns is still challenging in clinical practice. The use of free flow-through anterolateral thigh flaps (combined with great saphenous vein transplantation if necessary) to repair the wound and to restore the blood supply for the hand at the same time is a good choice for treating severe wrist electrical burns.
Progress in Understanding the Functional Roles of Extracellular Vesicles in Reproduction
HAN Ting Ting, LI Wei, LI Guo Ping
2020, 33(7): 518-527.   doi: 10.3967/bes2020.068
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Relationship between Exposure to Low Dose of x-ray and DNA Hypomethylation in Solid Tumors and Hematological Malignancies
2020, 33(7): 528-537.   doi: 10.3967/bes2020.069
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Letter to the Editor
Whole-Exome Sequencing Identifies Origin of Recurrent Hepatocellular Carcinoma after Liver Transplantation: A Case Report
SHU Qing Ming, CHEN Xin Guo, ZHANG Hong Ying, HUANG Li Hong, ZHANG Yong Liang, ZHANG Qing
2020, 33(7): 538-542.   doi: 10.3967/bes2020.070
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Verification of the Cut-off Value of the Reticulocyte Hemoglobin Content to Diagnose Iron Deficiency
ZHANG Hui Di, CAI Jie, WU Meng, REN Jie, DU Ya Li, LONG Zhang Biao, LI Guo Xun, HAN Bing, YANG Li Chen
2020, 33(7): 543-546.   doi: 10.3967/bes2020.071
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Relationship between Short-term Exposure to PM2.5 and Daily Lung Cancer Mortality in Nanjing
WEI Fei Ran, XIONG Li Lin, LI Wei, WANG Xue Rong, HONG Xin, CHEN Bao An
2020, 33(7): 547-551.   doi: 10.3967/bes2020.072
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Effect of Antiretroviral Therapy Medications for Acquired Immune Deficiency Syndrome on Serum Elemental Concentrations
ZHANG Peng, CHEN Cheng, LIU Li Ying
2020, 33(7): 552-555.   doi: 10.3967/bes2020.073
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The Serum Exosome Derived MicroRNA-135a, -193b, and-384 Were Potential Alzheimer's Disease Biomarkers
YANG Ting Ting, LIU Chen Geng, GAO Shi Chao, ZHANG Yi, WANG Pei Chang
2018, 31(2): 87-96.   doi: 10.3967/bes2018.011
[Abstract](2720) [FullText HTML](247) [PDF 11333KB](247)
Resveratrol Induces Apoptosis and Autophagy in T-cell Acute Lymphoblastic Leukemia Cells by Inhibiting Akt/mTOR and Activating p38-MAPK
GE Jiao, LIU Yan, LI Qiang, GUO Xia, GU Ling, MA Zhi Gui, ZHU Yi Ping
2013, 26(11): 902-911.   doi: 10.3967/bes2013.019
[Abstract](2220) [PDF 12143KB](142)
Stability of SARS Coronavirus in Human Specimens and Environment and Its Sensitivity to Heating and UV Irradiation
2003, 16(3): 246-255.  
[Abstract](3930) [PDF 0KB](278)
Inactivation of Poliovirus by Ozone and the Impact of Ozone on the Viral Genome
JIANG Han Ji, CHEN Na, SHEN Zhi Qiang, YIN Jing, QIU Zhi Gang, MIAO Jing, YANG Zhong Wei, SHI Dan Yang, WANG Hua Ran, WANG Xin Wei, LI Jun Wen, YANG Dong, JIN Min
2019, 32(5): 324-333.   doi: 10.3967/bes2019.044
[Abstract](2074) [FullText HTML](186) [PDF 2666KB](186)
An Evaluation of the Investment for Child Development in China
ZHENG Xiao Ying, CHEN Chun Ming, HUANG Cheng Li, Han You Li, QIU Yue, ZHANG Qian Deng, CHEN He
2012, 25(4): 413-420.   doi: 10.3967/0895-3988.2012.04.006
[Abstract](1861) [PDF 183KB](121)
Analysis of Protoscoleces-specific Antigens from Echinococcus Granulosus with Proteomics Combined with Western Blot
LI Zong Ji, ZHAO Wei
2012, 25(6): 718-723.   doi: 10.3967/0895-3988.2012.06.015
[Abstract](1748) [PDF 279KB](123)
Bone Injury and Fracture Healing Biology
Ahmad Oryan, Somayeh Monazzah, Amin Bigham-Sadegh
2015, 28(1): 57-71.   doi: 10.3967/bes2015.006
[Abstract](1546) [PDF 3875KB](237)
Metabolomic Profiling Differences among Asthma, COPD, and Healthy Subjects: A LC-MS-based Metabolomic Analysis
LIANG Ying, GAI Xiao Yan, CHANG Chun, ZHANG Xu, WANG Juan, LI Ting Ting
2019, 32(9): 659-672.   doi: 10.3967/bes2019.085
[Abstract](2086) [FullText HTML](191) [PDF 2914KB](191)
A Study of the Technique of Western Blot for Diagnosis of Lyme Disease caused by Borrelia afzelii in China
LIU Zhi Yun, HAO Qin, HOU Xue Xia, JIANG Yi, GENG Zhen, WU Yi Mou, WAN Kang Lin
2013, 26(3): 190-200.   doi: 10.3967/0895-3988.2013.03.006
[Abstract](2004) [PDF 9794KB](145)
Outline of the Report on Cardiovascular Disease in China, 2010
HU Sheng Shou, KONG Ling Zhi, GAO Run Lin, ZHU Man Lu, WANG Wen, WANG Yong Jun, WU Zhao Su, CHEN Wei Wei, LIU Ming Bo
2012, 25(3): 251-256.   doi: 10.3967/0895-3988.2012.03.001
[Abstract](2651) [PDF 2961KB](145)
Evaluating the Nutritional Status of Oncology Patientsand Its Association with Quality of Life
ZHANG Ya Hui, XIE Fang Yi, CHEN Ya Wen, WANG Hai Xia, TIAN Wen Xia, SUN Wen Guang, WU Jing
2018, 31(9): 637-644.   doi: 10.3967/bes2018.088
[Abstract](2477) [FullText HTML](189) [PDF 31943KB](189)
Interpretation Criteria for Standardized Western Blot for the Predominant Species of Borrelia Burgdorferi Sensu Lato in China
2010, 23(5): 341-349.  
[Abstract](1963) [PDF 314KB](123)
Viral and Bacterial Etiology of Acute Febrile Respiratory Syndrome among Patients in Qinghai, China
LIU Gao Shan, LI Hong, ZHAO Sheng Cang, LU Rou Jian, NIU Pei Hua, TAN Wen Jie
2019, 32(6): 438-445.   doi: 10.3967/bes2019.058
[Abstract](2229) [PDF 10063KB](184)
Predictive Values of Body Mass Index and Waist Circumference for Risk Factors of Certain Related Diseases in Chinese Adults - Study on Optimal Cut-off Points of Body Mass Index and Waist Circumference in Chinese Adults
ZHOU Bei-fan
2002, 15(1): 83-96.  
[Abstract](1979) [PDF 0KB](119)
Paraben Content in Adjacent Normal-malignant Breast Tissues from Women with Breast Cancer
Mohammad Mehdi Amin, Maryam Tabatabaeian, Afsane Chavoshani, Elham Amjadi, Majid Hashemi, Karim Ebrahimpour, Roya Klishadi, Sedigheh Khazaei, Marjan Mansourian
2019, 32(12): 893-904.   doi: 10.3967/bes2019.112
[Abstract](1396) [FullText HTML](151) [PDF 2461KB](151)
Evaluation of Effectiveness of Integrated Intervention Program in Improving Drug Addicts' Psychological Health1
2008, 21(1): 75-84.  
[Abstract](1570) [PDF 0KB](121)
Supplementation of Fermented Barley Extracts with Lactobacillus Plantarum dy-1 Inhibits Obesity via a UCP1-dependent Mechanism
XIAO Xiang, BAI Juan, LI Ming Song, ZHANG Jia Yan, SUN Xin Juan, DONG Ying
2019, 32(8): 578-591.   doi: 10.3967/bes2019.076
[Abstract](2513) [FullText HTML](173) [PDF 9101KB](173)
Report on Childhood Obesity in China (9): Sugar-sweetened Beverages Consumption and Obesity
SHANG Xian Wen, LIU Ai Ling, ZHANG Qian, HU Xiao Qi, DU Song Ming, MA Jun, XU Gui Fa, LI Ying, GUO Hong Wei, DU Lin, LI Ting Yu, MA Guan Sheng
2012, 25(2): 125-132.   doi: 10.3967/0895-3988.2012.02.001
[Abstract](1964) [PDF 460KB](123)
Exposure Effects of Terahertz Waves on Primary Neurons and Neuron-like Cells Under Nonthermal Conditions
TAN Sheng Zhi, TAN Peng Cheng, LUO Lan Qing, CHI Yun Liang, YANG Zi Long, ZHAO Xue Long, ZHAO Li, DONG Ji, ZHANG Jing, YAO Bin Wei, XU Xin Ping, TIAN Guang, CHEN Jian Kui, WANG Hui, PENG Rui Yun
2019, 32(10): 739-754.   doi: 10.3967/bes2019.094
[Abstract](1494) [FullText HTML](148) [PDF 3322KB](148)
Burden of Cirrhosis and Other Chronic Liver Diseases Caused by Specific Etiologies in China, 1990−2016: Findings from the Global Burden of Disease Study 2016
LI Man, WANG Zhuo Qun, ZHANG Lu, ZHENG Hao, LIU Dian Wu, ZHOU Mai Geng
2020, 33(1): 1-10.   doi: 10.3967/bes2020.001
[Abstract](1963) [FullText HTML](131) [PDF 2552KB](131)

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Current Issue

Vol 33, No 7

(July, 2020)

ISSN 0895-3988

CN 11-2816/Q

  • 2019 Impact Factor 2.656

  • 2019 Journal Citation Reports