SHEN YuJuan; YIN JianHai; YUAN ZhongYing; LU WeiYuan; XU YuXin; XIAO LiHua; CAO JianPing

doi:10.3967/0895-3988.2011.03.016

The Identification of the Cryptosporidium ubiquitum in Pre-weaned Ovines from Aba Tibetan and Qiang Autonomous Prefecture in China

SHEN YuJuan, YIN JianHai, YUAN ZhongYing, LU WeiYuan, XU YuXin, XIAO LiHua, CAO JianPing

文章导航 > Biomedical and Environmental Sciences > 2011 > 24(3): 315-320

SHEN YuJuan, YIN JianHai, YUAN ZhongYing, LU WeiYuan, XU YuXin, XIAO LiHua, CAO JianPing. The Identification of the Cryptosporidium ubiquitum in Pre-weaned Ovines from Aba Tibetan and Qiang Autonomous Prefecture in China[J]. Biomedical and Environmental Sciences, 2011, 24(3): 315-320. doi: 10.3967/0895-3988.2011.03.016

Citation:

SHEN YuJuan, YIN JianHai, YUAN ZhongYing, LU WeiYuan, XU YuXin, XIAO LiHua, CAO JianPing. The Identification of the Cryptosporidium ubiquitum in Pre-weaned Ovines from Aba Tibetan and Qiang Autonomous Prefecture in China[J]. Biomedical and Environmental Sciences, 2011, 24(3): 315-320. doi: 10.3967/0895-3988.2011.03.016

doi: 10.3967/0895-3988.2011.03.016

基金项目: the Chinese Special Program for Scientific Research of Public Health(200802012)%Chinese National Key Program for Infectious Diseases of China(2009ZX10004-201%%2008ZX10004-002)

The Identification of the Cryptosporidium ubiquitum in Pre-weaned Ovines from Aba Tibetan and Qiang Autonomous Prefecture in China

National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Laboratory of Parasite and Vector Biology, MOH, China, WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, Shanghai 200025, China
Centers for Disease Control and Prevention, Atlanta, Georgia, USA

Funds: the Chinese Special Program for Scientific Research of Public Health(200802012)%Chinese National Key Program for Infectious Diseases of China(2009ZX10004-201%%2008ZX10004-002)

摘要: Objective Cryptosporidium spp. are prevalent globally and sheep are an important zoonotic reservoir. Little data regarding the rates of Cryptosporidium infections in ovines in China are available. This study assessed the prevalence of Cryptosporidium spp. in pre-weaned ovines from Aba Tibetan and Qjang Autonomous Prefecture in the Sichuan province of China.Methods A total of 213 fecal samples were collected from pre-weaned ovines and were examined microscopically (following modified acid fast staining). In addition, 18S rRNA genetic sequences were amplified from fecal samples by nested PCR and phylogenetically analyzed.Results The prevalence of Cryptosporidium in the collected samples was at 14.6% (31/213) and four isolates identified by PCR belonged to the Cryptosporidium cervine genotype (Cryptosporidium ubiquitum) demonstrating that this species was the primary sheep species found in sheep in China.Conclusion The present study suggested that the high incidence of Cryptosporidium in sheep poses a significant public health threat and that surveillance practices must be established to prevent zoonotic disease of humans.
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Abstract: Objective Cryptosporidium spp. are prevalent globally and sheep are an important zoonotic reservoir. Little data regarding the rates of Cryptosporidium infections in ovines in China are available. This study assessed the prevalence of Cryptosporidium spp. in pre-weaned ovines from Aba Tibetan and Qjang Autonomous Prefecture in the Sichuan province of China.Methods A total of 213 fecal samples were collected from pre-weaned ovines and were examined microscopically (following modified acid fast staining). In addition, 18S rRNA genetic sequences were amplified from fecal samples by nested PCR and phylogenetically analyzed.Results The prevalence of Cryptosporidium in the collected samples was at 14.6% (31/213) and four isolates identified by PCR belonged to the Cryptosporidium cervine genotype (Cryptosporidium ubiquitum) demonstrating that this species was the primary sheep species found in sheep in China.Conclusion The present study suggested that the high incidence of Cryptosporidium in sheep poses a significant public health threat and that surveillance practices must be established to prevent zoonotic disease of humans.
- Cryptosporidium ubiquitum /
- Ovines /
- Aba /
- China /
- Cryptosporidium cervine genotype

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The Identification of the Cryptosporidium ubiquitum in Pre-weaned Ovines from Aba Tibetan and Qiang Autonomous Prefecture in China

doi: 10.3967/0895-3988.2011.03.016

基金项目: the Chinese Special Program for Scientific Research of Public Health(200802012)%Chinese National Key Program for Infectious Diseases of China(2009ZX10004-201%%2008ZX10004-002)

刊出日期: 2011-06-20

关键词:

Cryptosporidium ubiquitum /

Ovines /

Aba /

China /

Cryptosporidium cervine genotype

摘要: Objective Cryptosporidium spp. are prevalent globally and sheep are an important zoonotic reservoir. Little data regarding the rates of Cryptosporidium infections in ovines in China are available. This study assessed the prevalence of Cryptosporidium spp. in pre-weaned ovines from Aba Tibetan and Qjang Autonomous Prefecture in the Sichuan province of China.Methods A total of 213 fecal samples were collected from pre-weaned ovines and were examined microscopically (following modified acid fast staining). In addition, 18S rRNA genetic sequences were amplified from fecal samples by nested PCR and phylogenetically analyzed.Results The prevalence of Cryptosporidium in the collected samples was at 14.6% (31/213) and four isolates identified by PCR belonged to the Cryptosporidium cervine genotype (Cryptosporidium ubiquitum) demonstrating that this species was the primary sheep species found in sheep in China.Conclusion The present study suggested that the high incidence of Cryptosporidium in sheep poses a significant public health threat and that surveillance practices must be established to prevent zoonotic disease of humans.

English Abstract

The Identification of the Cryptosporidium ubiquitum in Pre-weaned Ovines from Aba Tibetan and Qiang Autonomous Prefecture in China

National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Laboratory of Parasite and Vector Biology, MOH, China, WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, Shanghai 200025, China

Centers for Disease Control and Prevention, Atlanta, Georgia, USA

Funds: the Chinese Special Program for Scientific Research of Public Health(200802012)%Chinese National Key Program for Infectious Diseases of China(2009ZX10004-201%%2008ZX10004-002)

Publish Date: 2011-06-20

Keywords:

Abstract: Objective Cryptosporidium spp. are prevalent globally and sheep are an important zoonotic reservoir. Little data regarding the rates of Cryptosporidium infections in ovines in China are available. This study assessed the prevalence of Cryptosporidium spp. in pre-weaned ovines from Aba Tibetan and Qjang Autonomous Prefecture in the Sichuan province of China.Methods A total of 213 fecal samples were collected from pre-weaned ovines and were examined microscopically (following modified acid fast staining). In addition, 18S rRNA genetic sequences were amplified from fecal samples by nested PCR and phylogenetically analyzed.Results The prevalence of Cryptosporidium in the collected samples was at 14.6% (31/213) and four isolates identified by PCR belonged to the Cryptosporidium cervine genotype (Cryptosporidium ubiquitum) demonstrating that this species was the primary sheep species found in sheep in China.Conclusion The present study suggested that the high incidence of Cryptosporidium in sheep poses a significant public health threat and that surveillance practices must be established to prevent zoonotic disease of humans.

Citation:

SHEN YuJuan, YIN JianHai, YUAN ZhongYing, LU WeiYuan, XU YuXin, XIAO LiHua, CAO JianPing. The Identification of the Cryptosporidium ubiquitum in Pre-weaned Ovines from Aba Tibetan and Qiang Autonomous Prefecture in China[J]. Biomedical and Environmental Sciences, 2011, 24(3): 315-320. doi: 10.3967/0895-3988.2011.03.016