GUO Xiang; YANG Xing Fen; YANG Ying; HANS Raabe; CAI Jing Heng; XUE Jin Yu; TAN Xiao Hua; XIE Xiao Ping; XIONG Xi Kun; HUANG Jun Ming

doi:10.3967/0895-3988.2012.03.015

Prediction of Ocular Irritancy of 26 Chemicals and 26 Cosmetic Products with Isolated Rabbit Eye (IRE) Test

GUO Xiang, YANG Xing Fen, YANG Ying, HANS Raabe, CAI Jing Heng, XUE Jin Yu, TAN Xiao Hua, XIE Xiao Ping, XIONG Xi Kun, HUANG Jun Ming

文章导航 > Biomedical and Environmental Sciences > 2012 > 25(3): 359-366

GUO Xiang, YANG Xing Fen, YANG Ying, HANS Raabe, CAI Jing Heng, XUE Jin Yu, TAN Xiao Hua, XIE Xiao Ping, XIONG Xi Kun, HUANG Jun Ming. Prediction of Ocular Irritancy of 26 Chemicals and 26 Cosmetic Products with Isolated Rabbit Eye (IRE) Test[J]. Biomedical and Environmental Sciences, 2012, 25(3): 359-366. doi: 10.3967/0895-3988.2012.03.015

Citation:

GUO Xiang, YANG Xing Fen, YANG Ying, HANS Raabe, CAI Jing Heng, XUE Jin Yu, TAN Xiao Hua, XIE Xiao Ping, XIONG Xi Kun, HUANG Jun Ming. Prediction of Ocular Irritancy of 26 Chemicals and 26 Cosmetic Products with Isolated Rabbit Eye (IRE) Test[J]. Biomedical and Environmental Sciences, 2012, 25(3): 359-366. doi: 10.3967/0895-3988.2012.03.015

doi: 10.3967/0895-3988.2012.03.015

基金项目: This work was supported by a grant from the Ministry of Health of the People's Republic of China for the Alternative to animals Testing project(200802080)

Prediction of Ocular Irritancy of 26 Chemicals and 26 Cosmetic Products with Isolated Rabbit Eye (IRE) Test

Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 510300, Guangdong,China; National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China
Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 510300, Guangdong,China
Institute for in vitro Science, Gaithersburg MD 20878,Maryland, USA
Faculty of Mathematics and Computational Science, Sun Yat-sen University,Guangzhou 510275, Guangdong, China

Funds: This work was supported by a grant from the Ministry of Health of the People's Republic of China for the Alternative to animals Testing project(200802080)

摘要: Objective This study aims to establish and evaluate the methodology of isolated rabbit eye (IRE) test.Methods IRE test was performed according to modifications of the in vitro toxicology (INVITTOX)Protocol No.85:Rabbit enucleated eye test by European Centre for the Validation of Alternative Methods (ECVAM),and then 26 chemicals and 26 cosmetic products were tested in both in vitro IRE and in vivo Draize tests.A statistical analysis was conducted to determine the relevance of the IRE test to the data generated in the Draize test.Results IRE test was established successfully in our laboratory.It was shown that ranking correlation and class concordance were fairly well between the IRE test and the Draize test for 26 reference chemicals (Fisher's Exact Test x2=51.314,P＜0.001; McNemar P=0.261; Gamma=0.960,P＜0.001;Kappa=0.843,P＜0.001) and 26 cosmetic products (Fisher's Exact Test x2=15.522,P＜0.001; McNemar P=0.311; Gamma=0.967,P＜0.001; Kappa=0.611,P＜0.001).Conclusion IRE test was established successfully for in vitro testing of eye irritation as an alternative to Draize test.
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Abstract: Objective This study aims to establish and evaluate the methodology of isolated rabbit eye (IRE) test.Methods IRE test was performed according to modifications of the in vitro toxicology (INVITTOX)Protocol No.85:Rabbit enucleated eye test by European Centre for the Validation of Alternative Methods (ECVAM),and then 26 chemicals and 26 cosmetic products were tested in both in vitro IRE and in vivo Draize tests.A statistical analysis was conducted to determine the relevance of the IRE test to the data generated in the Draize test.Results IRE test was established successfully in our laboratory.It was shown that ranking correlation and class concordance were fairly well between the IRE test and the Draize test for 26 reference chemicals (Fisher's Exact Test x2=51.314,P＜0.001; McNemar P=0.261; Gamma=0.960,P＜0.001;Kappa=0.843,P＜0.001) and 26 cosmetic products (Fisher's Exact Test x2=15.522,P＜0.001; McNemar P=0.311; Gamma=0.967,P＜0.001; Kappa=0.611,P＜0.001).Conclusion IRE test was established successfully for in vitro testing of eye irritation as an alternative to Draize test.
- Isolated rabbit eye /
- IRE /
- Eye irritation /
- Alternative testing /
- Draize test

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Prediction of Ocular Irritancy of 26 Chemicals and 26 Cosmetic Products with Isolated Rabbit Eye (IRE) Test

doi: 10.3967/0895-3988.2012.03.015

基金项目: This work was supported by a grant from the Ministry of Health of the People's Republic of China for the Alternative to animals Testing project(200802080)

刊出日期: 2012-06-20

关键词:

Isolated rabbit eye /

IRE /

Eye irritation /

Alternative testing /

Draize test

摘要: Objective This study aims to establish and evaluate the methodology of isolated rabbit eye (IRE) test.Methods IRE test was performed according to modifications of the in vitro toxicology (INVITTOX)Protocol No.85:Rabbit enucleated eye test by European Centre for the Validation of Alternative Methods (ECVAM),and then 26 chemicals and 26 cosmetic products were tested in both in vitro IRE and in vivo Draize tests.A statistical analysis was conducted to determine the relevance of the IRE test to the data generated in the Draize test.Results IRE test was established successfully in our laboratory.It was shown that ranking correlation and class concordance were fairly well between the IRE test and the Draize test for 26 reference chemicals (Fisher's Exact Test x2=51.314,P＜0.001; McNemar P=0.261; Gamma=0.960,P＜0.001;Kappa=0.843,P＜0.001) and 26 cosmetic products (Fisher's Exact Test x2=15.522,P＜0.001; McNemar P=0.311; Gamma=0.967,P＜0.001; Kappa=0.611,P＜0.001).Conclusion IRE test was established successfully for in vitro testing of eye irritation as an alternative to Draize test.

English Abstract

Prediction of Ocular Irritancy of 26 Chemicals and 26 Cosmetic Products with Isolated Rabbit Eye (IRE) Test

Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 510300, Guangdong,China; National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China

Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 510300, Guangdong,China

Institute for in vitro Science, Gaithersburg MD 20878,Maryland, USA

Faculty of Mathematics and Computational Science, Sun Yat-sen University,Guangzhou 510275, Guangdong, China

Funds: This work was supported by a grant from the Ministry of Health of the People's Republic of China for the Alternative to animals Testing project(200802080)

Publish Date: 2012-06-20

Keywords:

Abstract: Objective This study aims to establish and evaluate the methodology of isolated rabbit eye (IRE) test.Methods IRE test was performed according to modifications of the in vitro toxicology (INVITTOX)Protocol No.85:Rabbit enucleated eye test by European Centre for the Validation of Alternative Methods (ECVAM),and then 26 chemicals and 26 cosmetic products were tested in both in vitro IRE and in vivo Draize tests.A statistical analysis was conducted to determine the relevance of the IRE test to the data generated in the Draize test.Results IRE test was established successfully in our laboratory.It was shown that ranking correlation and class concordance were fairly well between the IRE test and the Draize test for 26 reference chemicals (Fisher's Exact Test x2=51.314,P＜0.001; McNemar P=0.261; Gamma=0.960,P＜0.001;Kappa=0.843,P＜0.001) and 26 cosmetic products (Fisher's Exact Test x2=15.522,P＜0.001; McNemar P=0.311; Gamma=0.967,P＜0.001; Kappa=0.611,P＜0.001).Conclusion IRE test was established successfully for in vitro testing of eye irritation as an alternative to Draize test.

Citation:

GUO Xiang, YANG Xing Fen, YANG Ying, HANS Raabe, CAI Jing Heng, XUE Jin Yu, TAN Xiao Hua, XIE Xiao Ping, XIONG Xi Kun, HUANG Jun Ming. Prediction of Ocular Irritancy of 26 Chemicals and 26 Cosmetic Products with Isolated Rabbit Eye (IRE) Test[J]. Biomedical and Environmental Sciences, 2012, 25(3): 359-366. doi: 10.3967/0895-3988.2012.03.015