YIN Jing Feng; WANG Jin Ling; TANG Qing; DING Yu Lin; TAO XiaoYan; LI Hao; SONG Miao; GUO ZhenYang; SHEN Xin Xin; LIANG Guo Dong; WANG Feng Long

doi:10.3967/bes2014.003

Identification of Animal Rabies in Inner Mongolia and Analysis of the Etiologic Characteristics

YIN Jing Feng, WANG Jin Ling, TANG Qing, DING Yu Lin, TAO XiaoYan, LI Hao, SONG Miao, GUO ZhenYang, SHEN Xin Xin, LIANG Guo Dong, WANG Feng Long

文章导航 > Biomedical and Environmental Sciences > 2014 > 27(1): 35-44

YIN Jing Feng, WANG Jin Ling, TANG Qing, DING Yu Lin, TAO XiaoYan, LI Hao, SONG Miao, GUO ZhenYang, SHEN Xin Xin, LIANG Guo Dong, WANG Feng Long. Identification of Animal Rabies in Inner Mongolia and Analysis of the Etiologic Characteristics[J]. Biomedical and Environmental Sciences, 2014, 27(1): 35-44. doi: 10.3967/bes2014.003

Citation:

YIN Jing Feng, WANG Jin Ling, TANG Qing, DING Yu Lin, TAO XiaoYan, LI Hao, SONG Miao, GUO ZhenYang, SHEN Xin Xin, LIANG Guo Dong, WANG Feng Long. Identification of Animal Rabies in Inner Mongolia and Analysis of the Etiologic Characteristics[J]. Biomedical and Environmental Sciences, 2014, 27(1): 35-44. doi: 10.3967/bes2014.003

doi: 10.3967/bes2014.003

基金项目: a grant from the National Department Public Benefit Research Foundation (201103032,200803014)

Identification of Animal Rabies in Inner Mongolia and Analysis of the Etiologic Characteristics

College of Veterinary Medicine, Inner Mongolia Agricultural University, Huhhot, 010018, Inner Mongolia, China
Key Laboratory for Medical Virology, Ministry of Health, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention IVDC, China CDC, Beijing 102206, China
State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention IVDC, China CDC, Beijing 102206, China

Funds: a grant from the National Department Public Benefit Research Foundation (201103032,200803014)

摘要: Objective To perform pathological observation and etiological identification of specimens collected from dairy cows, beef cattle and dogs which were suspected of rabies in Inner Mongolia in 2011, and analyze their etiological characteristics.
Methods Pathological observation was conducted on the brain specimens of three infected animals with Hematoxylin-Eosin staining, followed by confirmation using immunofluorescence and nested RT-PCR methods. Finally, phylogenetic analysis was conducted using the virus N gene sequence amplified from three specimens.
Results Eosinophilic and cytoplasmic inclusion bodies were seen in neuronal cells of the CNS; and rabies non-characteristic histopathological changes were also detected in the CNS. The three brain specimens were detected positive. N gene nucleotide sequence of these three isolates showed distinct sequence identity, therefore they fell into different groups in the phylogenetic analysis. N gene in the cow and dog had higher homology with that in Hebei isolate, but that in the beef cattle had higher homology with that in Mongolian lupine isolate and Russian red fox isolate.
Conclusion Rabies were observed in the dairy cow, beef cattle and canine in the farm in Inner Mongolia, in 2011, which led to a different etiologic characteristics of the epidemic situation.
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Abstract: Objective To perform pathological observation and etiological identification of specimens collected from dairy cows, beef cattle and dogs which were suspected of rabies in Inner Mongolia in 2011, and analyze their etiological characteristics.
Methods Pathological observation was conducted on the brain specimens of three infected animals with Hematoxylin-Eosin staining, followed by confirmation using immunofluorescence and nested RT-PCR methods. Finally, phylogenetic analysis was conducted using the virus N gene sequence amplified from three specimens.
Results Eosinophilic and cytoplasmic inclusion bodies were seen in neuronal cells of the CNS; and rabies non-characteristic histopathological changes were also detected in the CNS. The three brain specimens were detected positive. N gene nucleotide sequence of these three isolates showed distinct sequence identity, therefore they fell into different groups in the phylogenetic analysis. N gene in the cow and dog had higher homology with that in Hebei isolate, but that in the beef cattle had higher homology with that in Mongolian lupine isolate and Russian red fox isolate.
Conclusion Rabies were observed in the dairy cow, beef cattle and canine in the farm in Inner Mongolia, in 2011, which led to a different etiologic characteristics of the epidemic situation.
- Rabies /
- Cow /
- Histopathology /
- Phylogeny

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Identification of Animal Rabies in Inner Mongolia and Analysis of the Etiologic Characteristics

doi: 10.3967/bes2014.003

基金项目: a grant from the National Department Public Benefit Research Foundation (201103032,200803014)

刊出日期: 2014-01-20

关键词:

Rabies /

Cow /

Histopathology /

Phylogeny

摘要: Objective To perform pathological observation and etiological identification of specimens collected from dairy cows, beef cattle and dogs which were suspected of rabies in Inner Mongolia in 2011, and analyze their etiological characteristics.
Methods Pathological observation was conducted on the brain specimens of three infected animals with Hematoxylin-Eosin staining, followed by confirmation using immunofluorescence and nested RT-PCR methods. Finally, phylogenetic analysis was conducted using the virus N gene sequence amplified from three specimens.
Results Eosinophilic and cytoplasmic inclusion bodies were seen in neuronal cells of the CNS; and rabies non-characteristic histopathological changes were also detected in the CNS. The three brain specimens were detected positive. N gene nucleotide sequence of these three isolates showed distinct sequence identity, therefore they fell into different groups in the phylogenetic analysis. N gene in the cow and dog had higher homology with that in Hebei isolate, but that in the beef cattle had higher homology with that in Mongolian lupine isolate and Russian red fox isolate.
Conclusion Rabies were observed in the dairy cow, beef cattle and canine in the farm in Inner Mongolia, in 2011, which led to a different etiologic characteristics of the epidemic situation.

English Abstract

Identification of Animal Rabies in Inner Mongolia and Analysis of the Etiologic Characteristics

College of Veterinary Medicine, Inner Mongolia Agricultural University, Huhhot, 010018, Inner Mongolia, China

Key Laboratory for Medical Virology, Ministry of Health, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention IVDC, China CDC, Beijing 102206, China

State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention IVDC, China CDC, Beijing 102206, China

Funds: a grant from the National Department Public Benefit Research Foundation (201103032,200803014)

Publish Date: 2014-01-20

Keywords:

Rabies /
Cow /
Histopathology /
Phylogeny

Abstract: Objective To perform pathological observation and etiological identification of specimens collected from dairy cows, beef cattle and dogs which were suspected of rabies in Inner Mongolia in 2011, and analyze their etiological characteristics.
Methods Pathological observation was conducted on the brain specimens of three infected animals with Hematoxylin-Eosin staining, followed by confirmation using immunofluorescence and nested RT-PCR methods. Finally, phylogenetic analysis was conducted using the virus N gene sequence amplified from three specimens.
Results Eosinophilic and cytoplasmic inclusion bodies were seen in neuronal cells of the CNS; and rabies non-characteristic histopathological changes were also detected in the CNS. The three brain specimens were detected positive. N gene nucleotide sequence of these three isolates showed distinct sequence identity, therefore they fell into different groups in the phylogenetic analysis. N gene in the cow and dog had higher homology with that in Hebei isolate, but that in the beef cattle had higher homology with that in Mongolian lupine isolate and Russian red fox isolate.
Conclusion Rabies were observed in the dairy cow, beef cattle and canine in the farm in Inner Mongolia, in 2011, which led to a different etiologic characteristics of the epidemic situation.

Citation:

YIN Jing Feng, WANG Jin Ling, TANG Qing, DING Yu Lin, TAO XiaoYan, LI Hao, SONG Miao, GUO ZhenYang, SHEN Xin Xin, LIANG Guo Dong, WANG Feng Long. Identification of Animal Rabies in Inner Mongolia and Analysis of the Etiologic Characteristics[J]. Biomedical and Environmental Sciences, 2014, 27(1): 35-44. doi: 10.3967/bes2014.003