LIN Yong Xin; DING Zhi Yong; ZHOU Xiao Bin; LI Si Tao; XIE De Ming; LI Zhi Zhong; SUN Guo Dong

doi:10.3967/bes2015.001

In vitro and In vivo Evaluation of the Developed PLGA/HAp/Zein Scaffolds for Bone-Cartilage Interface Regeneration

LIN Yong Xin, DING Zhi Yong, ZHOU Xiao Bin, LI Si Tao, XIE De Ming, LI Zhi Zhong, SUN Guo Dong

文章导航 > Biomedical and Environmental Sciences > 2015 > 28(1): 1-12

LIN Yong Xin, DING Zhi Yong, ZHOU Xiao Bin, LI Si Tao, XIE De Ming, LI Zhi Zhong, SUN Guo Dong. In vitro and In vivo Evaluation of the Developed PLGA/HAp/Zein Scaffolds for Bone-Cartilage Interface Regeneration[J]. Biomedical and Environmental Sciences, 2015, 28(1): 1-12. doi: 10.3967/bes2015.001

Citation:

LIN Yong Xin, DING Zhi Yong, ZHOU Xiao Bin, LI Si Tao, XIE De Ming, LI Zhi Zhong, SUN Guo Dong. In vitro and In vivo Evaluation of the Developed PLGA/HAp/Zein Scaffolds for Bone-Cartilage Interface Regeneration[J]. Biomedical and Environmental Sciences, 2015, 28(1): 1-12. doi: 10.3967/bes2015.001

doi: 10.3967/bes2015.001

基金项目: This research was financially supported by the National Natural Science Foundation of China, No.31070862%Science and Technology Plan of Guangzhou, No.12C32071662%Research Foundation of Guangdong Provincial Bureau of Traditional Chinese Medicine, No.2013113%scientific research and cultivating Foundation of the First Clinical Medical College of Jinan University, No.2012103 and No.2013208

In vitro and In vivo Evaluation of the Developed PLGA/HAp/Zein Scaffolds for Bone-Cartilage Interface Regeneration

Department of 0rthopedics, the First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong, China
Department of Traumatology, HuiZhou Municipal Central Hospital, Huizhou 516000, Guangdong, China
Department of Pediatrics, the Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou 510655, Guangdong, China
Department of Biomedical Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510630, Guangdong, China

Funds: This research was financially supported by the National Natural Science Foundation of China, No.31070862%Science and Technology Plan of Guangzhou, No.12C32071662%Research Foundation of Guangdong Provincial Bureau of Traditional Chinese Medicine, No.2013113%scientific research and cultivating Foundation of the First Clinical Medical College of Jinan University, No.2012103 and No.2013208

摘要: Objective To investigate the effect of electronspun PLGA/HAp/Zein scaffolds on the repair of cartilage defects.
Methods The PLGA/HAp/Zein composite scaffolds were fabricated by electrospinning method. The physiochemical properties and biocompatibility of the scaffolds were separately characterized by scanning electron microscope (SEM), transmission electron microscope (TEM), and fourier transform infrared spectroscopy (FTIR), human umbilical cord mesenchymal stem cells (hUC-MSCs) culture and animal experiments.
Results The prepared PLGA/HAp/Zein scaffolds showed fibrous structure with homogenous distribution. hUC-MSCs could attach to and grow well on PLGA/HAp/Zein scaffolds, and there was no significant difference between cell proliferation on scaffolds and that without scaffolds (P>0.05). The PLGA/HAp/Zein scaffolds possessed excellent ability to promote in vivo cartilage formation. Moreover, there was a large amount of immature chondrocytes and matrix with cartilage lacuna on PLGA/HAp/Zein scaffolds.
Conclusion The data suggest that the PLGA/HAp/Zein scaffolds possess good biocompatibility, which are anticipated to be potentially applied in cartilage tissue engineering and reconstruction.
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Abstract: Objective To investigate the effect of electronspun PLGA/HAp/Zein scaffolds on the repair of cartilage defects.
Methods The PLGA/HAp/Zein composite scaffolds were fabricated by electrospinning method. The physiochemical properties and biocompatibility of the scaffolds were separately characterized by scanning electron microscope (SEM), transmission electron microscope (TEM), and fourier transform infrared spectroscopy (FTIR), human umbilical cord mesenchymal stem cells (hUC-MSCs) culture and animal experiments.
Results The prepared PLGA/HAp/Zein scaffolds showed fibrous structure with homogenous distribution. hUC-MSCs could attach to and grow well on PLGA/HAp/Zein scaffolds, and there was no significant difference between cell proliferation on scaffolds and that without scaffolds (P>0.05). The PLGA/HAp/Zein scaffolds possessed excellent ability to promote in vivo cartilage formation. Moreover, there was a large amount of immature chondrocytes and matrix with cartilage lacuna on PLGA/HAp/Zein scaffolds.
Conclusion The data suggest that the PLGA/HAp/Zein scaffolds possess good biocompatibility, which are anticipated to be potentially applied in cartilage tissue engineering and reconstruction.
- hUC-MSCs /
- Electrospun /
- PLGA/HAp/Zein grafts /
- Cartilage tissue engineering /
- Chondrocyte Biomed Environ Sci,2015 /
- 28(1):1-12

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In vitro and In vivo Evaluation of the Developed PLGA/HAp/Zein Scaffolds for Bone-Cartilage Interface Regeneration

doi: 10.3967/bes2015.001

刊出日期: 2015-01-20

关键词:

hUC-MSCs /

Electrospun /

PLGA/HAp/Zein grafts /

Cartilage tissue engineering /

Chondrocyte Biomed Environ Sci,2015 /

28(1):1-12

摘要: Objective To investigate the effect of electronspun PLGA/HAp/Zein scaffolds on the repair of cartilage defects.
Methods The PLGA/HAp/Zein composite scaffolds were fabricated by electrospinning method. The physiochemical properties and biocompatibility of the scaffolds were separately characterized by scanning electron microscope (SEM), transmission electron microscope (TEM), and fourier transform infrared spectroscopy (FTIR), human umbilical cord mesenchymal stem cells (hUC-MSCs) culture and animal experiments.
Results The prepared PLGA/HAp/Zein scaffolds showed fibrous structure with homogenous distribution. hUC-MSCs could attach to and grow well on PLGA/HAp/Zein scaffolds, and there was no significant difference between cell proliferation on scaffolds and that without scaffolds (P>0.05). The PLGA/HAp/Zein scaffolds possessed excellent ability to promote in vivo cartilage formation. Moreover, there was a large amount of immature chondrocytes and matrix with cartilage lacuna on PLGA/HAp/Zein scaffolds.
Conclusion The data suggest that the PLGA/HAp/Zein scaffolds possess good biocompatibility, which are anticipated to be potentially applied in cartilage tissue engineering and reconstruction.

English Abstract

In vitro and In vivo Evaluation of the Developed PLGA/HAp/Zein Scaffolds for Bone-Cartilage Interface Regeneration

Department of 0rthopedics, the First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong, China

Department of Traumatology, HuiZhou Municipal Central Hospital, Huizhou 516000, Guangdong, China

Department of Pediatrics, the Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou 510655, Guangdong, China

Department of Biomedical Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510630, Guangdong, China

Publish Date: 2015-01-20

Keywords:

Abstract: Objective To investigate the effect of electronspun PLGA/HAp/Zein scaffolds on the repair of cartilage defects.
Methods The PLGA/HAp/Zein composite scaffolds were fabricated by electrospinning method. The physiochemical properties and biocompatibility of the scaffolds were separately characterized by scanning electron microscope (SEM), transmission electron microscope (TEM), and fourier transform infrared spectroscopy (FTIR), human umbilical cord mesenchymal stem cells (hUC-MSCs) culture and animal experiments.
Results The prepared PLGA/HAp/Zein scaffolds showed fibrous structure with homogenous distribution. hUC-MSCs could attach to and grow well on PLGA/HAp/Zein scaffolds, and there was no significant difference between cell proliferation on scaffolds and that without scaffolds (P>0.05). The PLGA/HAp/Zein scaffolds possessed excellent ability to promote in vivo cartilage formation. Moreover, there was a large amount of immature chondrocytes and matrix with cartilage lacuna on PLGA/HAp/Zein scaffolds.
Conclusion The data suggest that the PLGA/HAp/Zein scaffolds possess good biocompatibility, which are anticipated to be potentially applied in cartilage tissue engineering and reconstruction.

Citation:

LIN Yong Xin, DING Zhi Yong, ZHOU Xiao Bin, LI Si Tao, XIE De Ming, LI Zhi Zhong, SUN Guo Dong. In vitro and In vivo Evaluation of the Developed PLGA/HAp/Zein Scaffolds for Bone-Cartilage Interface Regeneration[J]. Biomedical and Environmental Sciences, 2015, 28(1): 1-12. doi: 10.3967/bes2015.001