ZHAO Ran; WANG Bi; CAI Qing Tao; LI Xiao Xia; LIU Min; HU Dong; GUO Dong Bei; WANG Juan; FAN Chun

doi:10.3967/bes2016.014

Bioremediation of Hexavalent Chromium Pollution by Sporosarcina saromensis M52 Isolated from Offshore Sediments in Xiamen, China

ZHAO Ran, WANG Bi, CAI Qing Tao, LI Xiao Xia, LIU Min, HU Dong, GUO Dong Bei, WANG Juan, FAN Chun

文章导航 > Biomedical and Environmental Sciences > 2016 > 29(2): 127-136

ZHAO Ran, WANG Bi, CAI Qing Tao, LI Xiao Xia, LIU Min, HU Dong, GUO Dong Bei, WANG Juan, FAN Chun. Bioremediation of Hexavalent Chromium Pollution by Sporosarcina saromensis M52 Isolated from Offshore Sediments in Xiamen, China[J]. Biomedical and Environmental Sciences, 2016, 29(2): 127-136. doi: 10.3967/bes2016.014

Citation:

ZHAO Ran, WANG Bi, CAI Qing Tao, LI Xiao Xia, LIU Min, HU Dong, GUO Dong Bei, WANG Juan, FAN Chun. Bioremediation of Hexavalent Chromium Pollution by Sporosarcina saromensis M52 Isolated from Offshore Sediments in Xiamen, China[J]. Biomedical and Environmental Sciences, 2016, 29(2): 127-136. doi: 10.3967/bes2016.014

doi: 10.3967/bes2016.014

基金项目: theXiamen Science and Technology Project of China(3502Z20123003)%Fundamental Research Funds for the Central Universities of China(2011121006)%National Undergraduate Training Program for Innovation and Entrepreneurship(201510384140)

Bioremediation of Hexavalent Chromium Pollution by Sporosarcina saromensis M52 Isolated from Offshore Sediments in Xiamen, China

School of Public Health, Xiamen University, Xiamen 361102, Fujian, China
Shanghai Jinshan District Center for Disease Control and Prevention, Shanghai 201599, China
Department of Preventive Medicine, Xiamen Medical School, Xiamen 361008, Fujian, China

Funds: theXiamen Science and Technology Project of China(3502Z20123003)%Fundamental Research Funds for the Central Universities of China(2011121006)%National Undergraduate Training Program for Innovation and Entrepreneurship(201510384140)

摘要: ObjectiveCr(VI) removal from industrial effluents and sediments has attracted the attention of environmental researchers. In the present study, we aimed to isolate bacteria for Cr(VI) bioremediation from sediment samples and to optimize parameters of biodegradation. MethodsStrains with the ability to tolerate Cr(VI) were obtained by serial dilution and spread plate methods and characterized by morphology, 16S rDNA identification, and phylogenetic analysis. Cr(VI) was determined using the 1,5-diphenylcarbazide method, and the optimum pH and temperature for degradation were studied using a multiple-factor mixed experimental design. Statistical analysis methods were used to analyze the results. ResultsFifty-five strains were obtained, and one strain (Sporosarcina saromensisM52; patent application number: 201410819443.3) having the ability to tolerate 500 mg Cr(VI)/L wasselected to optimize the degradation conditions. M52 was found be able to efficiently remove 50-200 mg Cr(VI)/L in 24 h, achieving the highest removal efficiency at pH 7.0-8.5 and 35°C. Moreover, M52 could completely degrade 100 mg Cr(VI)/L at pH 8.0 and35 °C in 24 h. The mechanism involved in the reduction of Cr(VI) was considered to be bioreduction rather than absorption. ConclusionThe strong degradation ability ofS. saromensis M52 and its advantageous functional characteristics support the potential use of this organism for bioremediation ofheavy metal pollution.
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Abstract: ObjectiveCr(VI) removal from industrial effluents and sediments has attracted the attention of environmental researchers. In the present study, we aimed to isolate bacteria for Cr(VI) bioremediation from sediment samples and to optimize parameters of biodegradation. MethodsStrains with the ability to tolerate Cr(VI) were obtained by serial dilution and spread plate methods and characterized by morphology, 16S rDNA identification, and phylogenetic analysis. Cr(VI) was determined using the 1,5-diphenylcarbazide method, and the optimum pH and temperature for degradation were studied using a multiple-factor mixed experimental design. Statistical analysis methods were used to analyze the results. ResultsFifty-five strains were obtained, and one strain (Sporosarcina saromensisM52; patent application number: 201410819443.3) having the ability to tolerate 500 mg Cr(VI)/L wasselected to optimize the degradation conditions. M52 was found be able to efficiently remove 50-200 mg Cr(VI)/L in 24 h, achieving the highest removal efficiency at pH 7.0-8.5 and 35°C. Moreover, M52 could completely degrade 100 mg Cr(VI)/L at pH 8.0 and35 °C in 24 h. The mechanism involved in the reduction of Cr(VI) was considered to be bioreduction rather than absorption. ConclusionThe strong degradation ability ofS. saromensis M52 and its advantageous functional characteristics support the potential use of this organism for bioremediation ofheavy metal pollution.
- Hexavalent chromium /
- Sediment /
- Sporosarcina saromensis /
- Degradation

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Bioremediation of Hexavalent Chromium Pollution by Sporosarcina saromensis M52 Isolated from Offshore Sediments in Xiamen, China

doi: 10.3967/bes2016.014

刊出日期: 2016-02-20

关键词:

Hexavalent chromium /

Sediment /

Sporosarcina saromensis /

Degradation

摘要: ObjectiveCr(VI) removal from industrial effluents and sediments has attracted the attention of environmental researchers. In the present study, we aimed to isolate bacteria for Cr(VI) bioremediation from sediment samples and to optimize parameters of biodegradation. MethodsStrains with the ability to tolerate Cr(VI) were obtained by serial dilution and spread plate methods and characterized by morphology, 16S rDNA identification, and phylogenetic analysis. Cr(VI) was determined using the 1,5-diphenylcarbazide method, and the optimum pH and temperature for degradation were studied using a multiple-factor mixed experimental design. Statistical analysis methods were used to analyze the results. ResultsFifty-five strains were obtained, and one strain (Sporosarcina saromensisM52; patent application number: 201410819443.3) having the ability to tolerate 500 mg Cr(VI)/L wasselected to optimize the degradation conditions. M52 was found be able to efficiently remove 50-200 mg Cr(VI)/L in 24 h, achieving the highest removal efficiency at pH 7.0-8.5 and 35°C. Moreover, M52 could completely degrade 100 mg Cr(VI)/L at pH 8.0 and35 °C in 24 h. The mechanism involved in the reduction of Cr(VI) was considered to be bioreduction rather than absorption. ConclusionThe strong degradation ability ofS. saromensis M52 and its advantageous functional characteristics support the potential use of this organism for bioremediation ofheavy metal pollution.

English Abstract

Bioremediation of Hexavalent Chromium Pollution by Sporosarcina saromensis M52 Isolated from Offshore Sediments in Xiamen, China

School of Public Health, Xiamen University, Xiamen 361102, Fujian, China

Shanghai Jinshan District Center for Disease Control and Prevention, Shanghai 201599, China

Department of Preventive Medicine, Xiamen Medical School, Xiamen 361008, Fujian, China

Publish Date: 2016-02-20

Keywords:

Abstract: ObjectiveCr(VI) removal from industrial effluents and sediments has attracted the attention of environmental researchers. In the present study, we aimed to isolate bacteria for Cr(VI) bioremediation from sediment samples and to optimize parameters of biodegradation. MethodsStrains with the ability to tolerate Cr(VI) were obtained by serial dilution and spread plate methods and characterized by morphology, 16S rDNA identification, and phylogenetic analysis. Cr(VI) was determined using the 1,5-diphenylcarbazide method, and the optimum pH and temperature for degradation were studied using a multiple-factor mixed experimental design. Statistical analysis methods were used to analyze the results. ResultsFifty-five strains were obtained, and one strain (Sporosarcina saromensisM52; patent application number: 201410819443.3) having the ability to tolerate 500 mg Cr(VI)/L wasselected to optimize the degradation conditions. M52 was found be able to efficiently remove 50-200 mg Cr(VI)/L in 24 h, achieving the highest removal efficiency at pH 7.0-8.5 and 35°C. Moreover, M52 could completely degrade 100 mg Cr(VI)/L at pH 8.0 and35 °C in 24 h. The mechanism involved in the reduction of Cr(VI) was considered to be bioreduction rather than absorption. ConclusionThe strong degradation ability ofS. saromensis M52 and its advantageous functional characteristics support the potential use of this organism for bioremediation ofheavy metal pollution.

Citation:

ZHAO Ran, WANG Bi, CAI Qing Tao, LI Xiao Xia, LIU Min, HU Dong, GUO Dong Bei, WANG Juan, FAN Chun. Bioremediation of Hexavalent Chromium Pollution by Sporosarcina saromensis M52 Isolated from Offshore Sediments in Xiamen, China[J]. Biomedical and Environmental Sciences, 2016, 29(2): 127-136. doi: 10.3967/bes2016.014

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doi: 10.3967/bes2016.014

Bioremediation of Hexavalent Chromium Pollution by Sporosarcina saromensis M52 Isolated from Offshore Sediments in Xiamen, China

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Bioremediation of Hexavalent Chromium Pollution by Sporosarcina saromensis M52 Isolated from Offshore Sediments in Xiamen, China

doi: 10.3967/bes2016.014

English Abstract

Bioremediation of Hexavalent Chromium Pollution by Sporosarcina saromensis M52 Isolated from Offshore Sediments in Xiamen, China

School of Public Health, Xiamen University, Xiamen 361102, Fujian, China Shanghai Jinshan District Center for Disease Control and Prevention, Shanghai 201599, China Department of Preventive Medicine, Xiamen Medical School, Xiamen 361008, Fujian, China

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School of Public Health, Xiamen University, Xiamen 361102, Fujian, China

Shanghai Jinshan District Center for Disease Control and Prevention, Shanghai 201599, China

Department of Preventive Medicine, Xiamen Medical School, Xiamen 361008, Fujian, China