LI Hao; GUO ZhenYang; ZHANG Jian; TAO XiaoYan; ZHU WuYang; TANG Qing; LIU HongTu

doi:10.3967/bes2016.044

Whole Genome Sequencing and Comparisons of Different Chinese Rabies Virus Lineages Including the First Complete Genome of an Arctic-like Strain in China

LI Hao, GUO ZhenYang, ZHANG Jian, TAO XiaoYan, ZHU WuYang, TANG Qing, LIU HongTu

文章导航 > Biomedical and Environmental Sciences > 2016 > 29(5): 340-346

LI Hao, GUO ZhenYang, ZHANG Jian, TAO XiaoYan, ZHU WuYang, TANG Qing, LIU HongTu. Whole Genome Sequencing and Comparisons of Different Chinese Rabies Virus Lineages Including the First Complete Genome of an Arctic-like Strain in China[J]. Biomedical and Environmental Sciences, 2016, 29(5): 340-346. doi: 10.3967/bes2016.044

Citation:

LI Hao, GUO ZhenYang, ZHANG Jian, TAO XiaoYan, ZHU WuYang, TANG Qing, LIU HongTu. Whole Genome Sequencing and Comparisons of Different Chinese Rabies Virus Lineages Including the First Complete Genome of an Arctic-like Strain in China[J]. Biomedical and Environmental Sciences, 2016, 29(5): 340-346. doi: 10.3967/bes2016.044

doi: 10.3967/bes2016.044

基金项目: the National Science and Technology Major Project of the Ministry of Science and Technology of China (2012ZX10004215)

Whole Genome Sequencing and Comparisons of Different Chinese Rabies Virus Lineages Including the First Complete Genome of an Arctic-like Strain in China

Key Laboratory for Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention, Beijing 102206, China

Funds: the National Science and Technology Major Project of the Ministry of Science and Technology of China (2012ZX10004215)

摘要: ObjectiveTo learn the rabies genome molecular characteristics and compare the difference of China rabies lineages.
MethodsThe complete genomes of 12 strains from different China rabies lineages were amplified and sequenced, and all the China street strain genomes (total 43), Arctic and Arctic-like genomes were aligned using ClustalX2, the genome homologies were analyzed using MegAlign software, and the phylogenetic trees were constructed by MEGA 5.
ResultsFirst Arctic-like rabies genome in China (CQH1202D) was reported, and we supplemented the rabies genome data of China, ensuring at least one genome was available in each China lineage. The genome size of China V (11908nt) is obviously shorter than other lineages’ (11923-11925nt) for the difference of N-P non-coding regions. Among different lineages, the genome homologies are almost under 90%.CQH1202D (China IV lineage) has close relationship with strains from South Korea and they share about 95% genome similarities.
ConclusionThe molecular characteristics of 6 different China rabies lineages werecompared and analyzed from genome level, which benefits for continued comprehensive rabies surveillance, rabies prevention and control in China.
- /
- /
Abstract: ObjectiveTo learn the rabies genome molecular characteristics and compare the difference of China rabies lineages.
MethodsThe complete genomes of 12 strains from different China rabies lineages were amplified and sequenced, and all the China street strain genomes (total 43), Arctic and Arctic-like genomes were aligned using ClustalX2, the genome homologies were analyzed using MegAlign software, and the phylogenetic trees were constructed by MEGA 5.
ResultsFirst Arctic-like rabies genome in China (CQH1202D) was reported, and we supplemented the rabies genome data of China, ensuring at least one genome was available in each China lineage. The genome size of China V (11908nt) is obviously shorter than other lineages’ (11923-11925nt) for the difference of N-P non-coding regions. Among different lineages, the genome homologies are almost under 90%.CQH1202D (China IV lineage) has close relationship with strains from South Korea and they share about 95% genome similarities.
ConclusionThe molecular characteristics of 6 different China rabies lineages werecompared and analyzed from genome level, which benefits for continued comprehensive rabies surveillance, rabies prevention and control in China.
- Rabies virus /
- Genome /
- Lineage

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Whole Genome Sequencing and Comparisons of Different Chinese Rabies Virus Lineages Including the First Complete Genome of an Arctic-like Strain in China

doi: 10.3967/bes2016.044

基金项目: the National Science and Technology Major Project of the Ministry of Science and Technology of China (2012ZX10004215)

刊出日期: 2016-05-20

关键词:

Rabies virus /

Genome /

Lineage

摘要: ObjectiveTo learn the rabies genome molecular characteristics and compare the difference of China rabies lineages.
MethodsThe complete genomes of 12 strains from different China rabies lineages were amplified and sequenced, and all the China street strain genomes (total 43), Arctic and Arctic-like genomes were aligned using ClustalX2, the genome homologies were analyzed using MegAlign software, and the phylogenetic trees were constructed by MEGA 5.
ResultsFirst Arctic-like rabies genome in China (CQH1202D) was reported, and we supplemented the rabies genome data of China, ensuring at least one genome was available in each China lineage. The genome size of China V (11908nt) is obviously shorter than other lineages’ (11923-11925nt) for the difference of N-P non-coding regions. Among different lineages, the genome homologies are almost under 90%.CQH1202D (China IV lineage) has close relationship with strains from South Korea and they share about 95% genome similarities.
ConclusionThe molecular characteristics of 6 different China rabies lineages werecompared and analyzed from genome level, which benefits for continued comprehensive rabies surveillance, rabies prevention and control in China.

English Abstract

Whole Genome Sequencing and Comparisons of Different Chinese Rabies Virus Lineages Including the First Complete Genome of an Arctic-like Strain in China

Key Laboratory for Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention, Beijing 102206, China

Funds: the National Science and Technology Major Project of the Ministry of Science and Technology of China (2012ZX10004215)

Publish Date: 2016-05-20

Keywords:

Rabies virus /
Genome /
Lineage

Abstract: ObjectiveTo learn the rabies genome molecular characteristics and compare the difference of China rabies lineages.
MethodsThe complete genomes of 12 strains from different China rabies lineages were amplified and sequenced, and all the China street strain genomes (total 43), Arctic and Arctic-like genomes were aligned using ClustalX2, the genome homologies were analyzed using MegAlign software, and the phylogenetic trees were constructed by MEGA 5.
ResultsFirst Arctic-like rabies genome in China (CQH1202D) was reported, and we supplemented the rabies genome data of China, ensuring at least one genome was available in each China lineage. The genome size of China V (11908nt) is obviously shorter than other lineages’ (11923-11925nt) for the difference of N-P non-coding regions. Among different lineages, the genome homologies are almost under 90%.CQH1202D (China IV lineage) has close relationship with strains from South Korea and they share about 95% genome similarities.
ConclusionThe molecular characteristics of 6 different China rabies lineages werecompared and analyzed from genome level, which benefits for continued comprehensive rabies surveillance, rabies prevention and control in China.

Citation:

LI Hao, GUO ZhenYang, ZHANG Jian, TAO XiaoYan, ZHU WuYang, TANG Qing, LIU HongTu. Whole Genome Sequencing and Comparisons of Different Chinese Rabies Virus Lineages Including the First Complete Genome of an Arctic-like Strain in China[J]. Biomedical and Environmental Sciences, 2016, 29(5): 340-346. doi: 10.3967/bes2016.044

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doi: 10.3967/bes2016.044

Whole Genome Sequencing and Comparisons of Different Chinese Rabies Virus Lineages Including the First Complete Genome of an Arctic-like Strain in China

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Whole Genome Sequencing and Comparisons of Different Chinese Rabies Virus Lineages Including the First Complete Genome of an Arctic-like Strain in China

doi: 10.3967/bes2016.044

English Abstract

Whole Genome Sequencing and Comparisons of Different Chinese Rabies Virus Lineages Including the First Complete Genome of an Arctic-like Strain in China

Key Laboratory for Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention, Beijing 102206, China

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