-
Overall, 2008 participants were included in the analysis, and the numbers of children aged 3, 4, and 5 years were 642, 700, and 666, respectively (Figure 1). The characteristics of the children and their parents are presented in Table 1. Approximately 89.2% of the children were ever breastfed and the median duration of breastfeeding was 12 months (IQR, 7–15 months). As the children grew, their height, weight, total body water, fat mass, and fat-free mass gradually increased (all P < 0.001; Table 2). Body composition indicators were stratified by age for subsequent analyses.
Table 1. Characteristics of children and parents
Characteristics N (%) or Mean ± SD Children Age groups (years) 3 32.0 (642/2,008) 4 34.8 (700/2,008) 5 33.2 (666/2,008) Gender Boy 50.2 (1,008/2,008) Girl 49.8 (1,000/2,008) Residential area Urban 54.9 (1,102/2,008) Rural 45.1 (906/2,008) Region North 55.1 (1,106/2,008) South 44.9 (902/2,008) Survey site Taigu County 18.0 (362/2,008) Jichang District 17.9 (360/2,008) Yongji County 19.1 (384/2,008) Qingshan Lake District 17.6 (354/2,008) Yuelu District 19.4 (388/2,008) Longchuan County 8.0 (160/2,008) Annual household income (per capita CNY*) ˂ 10,000 4.2 (85/2,004) 10,000– 7.3 (146/2,004) ≥ 20,000 12.2 (245/2,004) Refusal to answer 41.3 (828/2,004) Unknown 34.9 (700/2,004) Birth weight (g) < 2,500 2.6 (47/1,814) 2,500–4,000 90.7 (1,645/1,814) > 4,000 6.7 (122/1,814) Gestational age pre-term 4.6 (92/1,997) Term 95.4 (1,905/1,997) Nutrition status Stunting 1.1 (22/2,008) Wasting 1.7 (34/2,008) Underweight 0.8 (17/2,008) Overweight or obesity 9.6 (193/2,008) Breastfeeding Never 10.8 (217/2,007) Ever 89.2 (1,790/2,007) Duration of breastfeeding (months) Never 11.2 (217/1,931) < 4 11.4 (221/1,931) 4– 3.6 (69/1,931) 6– 28.4 (548/1,931) 12– 41.4 (800/1,931) ≥ 24 3.9 (76/1,931) Middle/high-level physical activity time (min) ≥ 60 81.0 (1,467/1,811) < 60 19.0 (344/1,811) Nocturnal sleep time (hours) 9.5 ± 0.6 Eating behavior scores 19.2 ± 3.4 Diet quality distance (scores) 22.6 ± 8.9 Parents Maternal BMI (kg/m2) Underweight 9.4 (180/1,912) Normal 72.2 (1,381/1,912) Overweight 15.1 (288/1,912) Obesity 3.3 (63/1,912) Maternal education Junior high school or below 27.0 (537/1,988) High school 18.5 (368/1,988) Associate’s degree 22.6 (450/1,988) Bachelor’s degree or above 31.8 (633/1,988) Father’s BMI (kg/m2) Underweight 3.0 (56/1,868) Normal 56.6 (1,057/1,868) Overweight 33.3 (622/1,868) Obesity 7.1 (133/1,868) Father’s education Junior high school or below 29.1 (580/1,996) High school 19.5 (390/1,996) Associate’s degree 19.9 (397/1,996) Bachelor’s degree or above 31.5 (629/1,996) Note. *CNY: Chinese Yuan. Table 2. Status of body composition and anthropometric measurements for children aged 3 to 5 years
Variables Total 3 years 4 years 5 years n M (IQR) or
Mean ± SDn M (IQR) or
Mean ± SDn M (IQR) or
Mean ± SDn M (IQR) or
Mean ± SDBody composition Total body water (kg) 2,007 10.8 ± 1.7 641 9.5 ± 1.1 700 10.7 ± 1.3 666 12.2 ± 1.5 Total fat-free mass (kg) 1,999 14.6 ± 2.3 641 12.8 ± 1.5 700 14.6 ± 1.7 658 16.5 ± 1.9 Fat-free mass index (kg/m2.5) 1,999 12.3 ± 0.8 641 12.8 ± 0.8 700 12.3 ± 0.7 658 11.9 ± 0.7 Total body fat mass (kg) 1,968 3.0 (2.4, 4.0) 632 2.7 (2.1, 3.5) 693 3.2 (2.5, 4.0) 643 3.3 (2.6, 4.5) Fat mass index (kg/m2) 1,968 2.7 (2.1, 3.5) 632 2.7 (2.2, 3.4) 693 2.8 (2.2, 3.5) 643 2.6 (2.1, 3.5) Percentage body fat (%) 1,968 18.2 ± 5.5 632 18.1 ± 5.1 693 18.6 ± 5.5 643 18.1 ± 6.0 Anthropometric status* Height (cm) 2,008 107.1 ± 7.4 642 99.9 ± 4.6 700 107.1 ± 4.9 666 114.0 ± 4.9 Weight (kg) 2,002 17.4 (15.6, 19.6) 642 15.3 (14.2, 16.6) 700 17.5 (16.0, 19.3) 660 19.6 (18.0, 22.1) BMI (kg/m2) 2,002 15.4 (14.6, 16.3) 642 15.4 (14.7, 16.2) 700 15.4 (14.6, 16.3) 660 15.3 (14.5, 16.5) WAZ 2,002 0.2 ± 1.1 642 0.1 ± 1.0 700 0.2 ± 1.0 660 0.3 ± 1.2 HAZ 2,008 0.2 ± 1.0 642 0.1 ± 1.0 700 0.1 ± 1.0 666 0.3 ± 1.0 WHZ 1,342 0.1 ± 1.0 642 0.1 ±1.0 700 0.1 ± 1.0 / / BAZ 2,002 0.1 ± 1.1 642 0.1 ±1.0 700 0.2 ± 1.0 660 0.2 ± 1.3 Note. *HAZ: Height-for-age Z-score, WAZ: Weight-for-age Z-score, WHZ: Weight-for-height Z-score, BAZ: BMI-for-age Z-score. -
After adjusting for gender, birth weight, survey sites, nocturnal sleep time, middle- and high-level physical activity time, eating behavior score, balanced diet index score, parental BMI, and education level, HAZ was lower in ever breastfed children than in never breastfed children (0.12 vs. 0.42, F = 4.14, P = 0.043) at 3 years of age (Table 3). In addition, WAZ was lower in ever breastfed children than in never breastfed children (0.31 vs. 0.65, F = 4.99, P = 0.026) at 4 years of age. The difference in the WAZ for different breastfeeding durations was statistically significant (F = 2.43, P = 0.034), and the WAZ was lower in children who breastfed for 12–23 months than in those who never breastfed (0.21 vs. 0.66) among children aged 4 years. No statistically significant difference in WHZ/BAZ was observed among children with different breastfeeding durations (Table 4).
Table 3. Relationship between breastfeeding duration and HAZ and WAZ of participants stratified by age
Variables n Height-for-age Z-score (HAZ) Weight-for-age Z-score (WAZ) Adjusted* Difference
(95% CI)Adjusted** Difference
(95% CI)Adjusted* Difference
(95% CI)Adjusted** Difference
(95% CI)Children aged 3 years Breastfeeding 642 Never 72 0.27 Referent 0.42 Referent 0.15 Referent 0.25 Referent Ever 570 0.12 −0.15
(−0.41, 0.10)0.12 −0.30
(−0.58, −0.01)0.14 0
(−0.25, 0.24)0.21 −0.03
(−0.30, 0.23)F value 1.46 4.14 0 0.06 P value 0.228 0.043 0.969 0.799 Breastfeeding duration (months) 623 Never 72 0.27 Referent 0.41 Referent 0.15 Referent 0.24 Referent < 4 68 0 −0.27
(−0.71, 0.16)−0.03 −0.43
(−0.94, 0.08)0.13 −0.02
(−0.44, 0.40)0.27 0.03
(−0.44, 0.49)4– 17 0.66 0.39
(−0.31, 1.08)0.38 −0.02
(−0.81, 0.76)0.50 0.36
(−0.32, 1.03)0.32 0.07
(−0.65, 0.79)6– 174 0.21 −0.06
(−0.42, 0.30)0.23 −0.18
(−0.60, 0.25)0.18 0.03
(−0.32, 0.38)0.24 0
(−0.39, 0.39)12– 266 0.03 −0.24
(−0.59, 0.10)0.05 −0.36
(−0.76, 0.05)0.07 −0.07
(−0.40, 0.26)0.19 −0.06
(−0.43, 0.31)≥ 24 26 0.16 −0.12
(−0.70, 0.47)−0.29 −0.69
(−1.41, 0.03)0.34 0.20
(−0.37, 0.77)−0.08 −0.33
(−0.99, 0.34)F value 2.15 2.19 0.96 0.44 P value 0.058 0.054 0.440 0.819 Children aged 4 years Breastfeeding 699 Never 71 0.38 Referent 0.40 Referent 0.46 Referent 0.65 Referent Ever 628 0.10 −0.28
(−0.51, −0.04)0.12 −0.28
(−0.58, 0.01)0.15 −0.31
(−0.55, −0.07)0.31 −0.34
(−0.64, −0.04)F value 5.21 3.60 6.22 4.99 P value 0.023 0.058 0.013 0.026 Breastfeeding duration (months) 663 Never 71 0.38 Referent 0.41 Referent 0.46 Referent 0.66 Referent < 4 80 0.21 −0.17
(−0.56, 0.22)0.29 −0.12
(−0.60, 0.35)0.21 −0.25
(−0.65, 0.15)0.44 −0.22
(−0.70, 0.26)4– 24 0.10 −0.28
(−0.85, 0.29)0.14 −0.28
(−0.93, 0.37)0.15 −0.31
(−0.89, 0.28)0.29 −0.37
(−1.03, 0.29)6– 196 0.16 −0.22
(−0.55, 0.12)0.23 −0.18
(−0.60, 0.24)0.20 −0.26
(−0.60, 0.08)0.48 −0.18
(−0.61, 0.24)12– 264 0.07 −0.30
(−0.62, 0.02)0.03 −0.38
(−0.78, 0.02)0.15 −0.31
(−0.64, 0.02)0.21 −0.45
(−0.85, −0.05)≥ 24 28 0.10 −0.27
(−0.81, 0.26)−0.06 −0.47
(−1.09, 0.14)0.13 −0.33
(−0.88, 0.22)0.14 −0.52
(−1.15, 0.10)F value 1.23 1.84 1.18 2.43 P value 0.292 0.105 0.315 0.034 Children aged 5 years Breastfeeding 666 Never 74 0.48 Referent 0.40 Referent 0.53 Referent 0.33 Referent Ever 592 0.32 −0.16
(−0.39, 0.06)0.34 −0.06
(−0.35, 0.24)0.13 −0.22
(−0.49, 0.06)0.34 0.02
(−0.34, 0.37)F value 1.96 0.14 2.46 0.01 P value 0.162 0.709 0.117 0.925 Breastfeeding duration (months) 645 Never 74 0.48 Referent 0.40 Referent 0.53 Referent 0.36 Referent < 4 73 0.51 0.03
(−0.36, 0.42)0.67 0.27
(−0.22, 0.75)0.37 −0.16
(−0.63, 0.31)0.64 0.28
(−0.30, 0.87)4– 28 0.42 0.06
(−0.59, 0.46)0.42 0.01
(−0.60, 0.63)0.53 0
(−0.64, 0.63)0.68 0.32
(−0.42, 1.06)6– 178 0.35 −0.13
(−0.46, 0.19)0.39 −0.01
(−0.44, 0.41)0.28 −0.26
(−0.65, 0.14)0.25 −0.10
(−0.62, 0.41)12– 270 0.22 −0.27
(−0.58, 0.04)0.25 −0.15
(−0.55, 0.24)0.29 −0.25
(−0.62, 0.13)0.34 −0.02
(−0.49, 0.46)≥ 24 22 0.41 −0.07
(−0.65, 0.50)0.39 −0.01
(−0.67, 0.65)0.16 −0.37
(−1.07, 0.32)0.23 −0.13
(−0.92, 0.67)F value 1.82 1.69 0.92 1.28 P value 0.106 0.135 0.470 0.273 Note. *Mean value after adjusting for gender. **Mean values after adjusting for gender, birth weight, survey site, nocturnal sleep time, middle- and high-level physical activity time, eating behavior score, balanced diet index score, parental BMI, and education level. Table 4. Relationship between breastfeeding duration and WHZ or BAZ of participants stratified by age
Variables n Weight-for-height Z-score (WHZ) or BMI-for-age Z-score (BAZ) Adjusted* Difference (95% CI) Adjusted** Difference (95% CI) Children aged 3 years Breastfeeding 642 Never 72 −0.02 Referent 0.001 Referent Ever 570 0.11 −0.08 0.21 0.21 (−0.07, 0.49) F value 1.00 0.13 (−0.12, 0.37) 2.08 P value 0.317 0.150 Breastfeeding duration (months) 623 Never 72 −0.02 Referent 0.01 Referent < 4 68 0.19 0.21 (−0.22, 0.64) 0.42 0.42 (−0.08, 0.91) 4– 17 0.19 0.21 (−0.48, 0.89) 0.14 0.13 (−0.63, 0.89) 6– 174 0.08 0.10 (−0.26, 0.45) 0.16 0.15 (−0.26, 0.56) 12– 266 0.09 0.37 (−0.21, 0.95) 0.22 0.22 (−0.17, 0.61) ≥ 24 26 0.35 0.10 0.09 (−0.61, 0.80) F value 0.70 1.03 P value 0.626 0.399 Children aged 4 years Breastfeeding 699 Never 71 0.28 Referent 0.55 Referent Ever 628 0.10 −0.18 (−0.43, 0.07) 0.32 −0.23 (−0.54, 0.09) F value 2.09 1.94 P value 0.149 0.164 Breastfeeding duration (months) 663 Never 71 0.28 Referent 0.56 Referent < 4 80 0.10 −0.19 (−0.60, 0.23) 0.37 −0.20 (−0.71, 0.32) 4– 24 0.09 −0.19 (−0.80, 0.41) 0.27 −0.29 (−1.00, 0.42) 6– 196 0.12 −0.17 (−0.52, 0.19) 0.47 −0.09 (−0.54, 0.37) 12– 264 0.12 −0.16 (−0.51, 0.18) 0.26 −0.30 (−0.74, 0.13) ≥ 24 28 0.05 −0.23 (−0.81, 0.34) 0.22 −0.34 (−1.01, 0.33) F value 0.39 1.10 P value 0.856 0.361 Children aged 5 years Breastfeeding 666 Never 74 0.31 Referent 0.08 Referent Ever 592 0.14 −0.17 (−0.47, 0.13) 0.17 0.09 (−0.30, 0.49) F value 1.26 0.21 P value 0.262 0.649 Breastfeeding duration (months) 645 Never 74 0.32 Referent 0.12 Referent < 4 73 0.08 −0.24 (−0.76, 0.27) 0.33 0.21 (−0.44, 0.86) 4– 28 0.40 0.08 (−0.61, 0.77) 0.62 0.50 (−0.32, 1.33) 6– 178 0.07 −0.25 (−0.68, 0.18) −0.01 −0.13 (−0.70, 0.44) 12– 270 0.20 −0.12 (−0.53, 0.29) 0.25 0.13 (−0.40, 0.66) ≥ 24 22 −0.23 −0.55 (−1.30, 0.21) −0.12 −0.24 (−1.13, 0.64) F value 1.21 1.58 P value 0.301 0.166 Note. * Mean value after adjusting for gender. **Mean value after adjusting for gender, birthweight, survey site, nocturnal sleep time, middle- and high-level physical activity time, eating behavior score, balanced diet index score, parental BMI, and education level. -
After adjusting for gender, birth weight, survey sites, HAZ (fat-free mass index was not adjusted by HAZ), nocturnal sleep time, middle- and high-level physical activity time, eating behavior score, balanced diet index score, parental BMI, and education level, there were no significant differences in fat-free mass index among the ever breastfed and never breastfed groups and different breastfeeding duration groups for children aged 3–5 years (Table 5).
Table 5. Relationship between breastfeeding status and fat-free mass and fat-free mass index of children stratified by age
Variables n Fat-free mass, kg Fat-free mass index, kg/m2.5 Adjusted* Difference
(95% CI)Adjusted** Difference
(95% CI)Adjusted* Difference
(95% CI)Adjusted** Difference
(95% CI)Children aged 3 years Breastfeeding 641 Never 71 12.72 Referent 12.52 Referent 12.70 Referent 12.62 Referent Ever 570 12.75 0.03
(−0.32, 0.38)12.84 0.32
(0.06, 0.59)12.75 0.05
(−0.14, 0.24)12.76 0.14
(−0.09, 0.37)F value 0.03 5.91 0.31 1.50 P value 0.868 0.015 0.580 0.222 Breastfeeding duration (months) 622 Never 71 12.72 Referent 12.51 Referent 12.70 Referent 12.63 Referent < 4 68 12.76 0.03
(−0.57, 0.64)12.98 0.46
(0.01, 0.92)12.74 0.04
(−0.29, 0.37)12.83 0.21
(−0.19, 0.61)4– 17 13.62 0.89
(−0.07, 1.86)13.06 0.54
(−0.16, 1.24)12.74 0.04
(−0.49, 0.56)12.70 0.07
(−0.54, 0.69)6– 174 12.85 0.13
(−0.37, 0.63)12.85 0.33
(−0.05, 0.71)12.74 0.04
(−0.23, 0.31)12.74 0.11
(−0.22, 0.44)12– 266 12.63 −0.10
(−0.58, 0.38)12.85 0.33
(−0.03, 0.69)12.77 0.07
(−0.18, 0.33)12.81 0.19
(−0.13, 0.51)≥ 24 26 12.76 0.03
(−0.78, 0.85)12.57 0.06
(−0.59, 0.71)12.77 0.08
(−0.37, 0.52)12.74 0.12
(−0.45, 0.68)F value 1.88 1.94 0.13 0.59 P value 0.096 0.086 0.986 0.711 Children aged 4 years Breastfeeding 699 Never 71 15.20 Referent 14.64 Referent 12.25 Referent 12.17 Referent Ever 628 14.49 −0.71
(−1.12, −0.30)14.31 −0.33
(−0.66, −0.003)12.25 −0.004
(−0.17, 0.16)12.13 −0.04
(−0.26, 0.18)F value 11.79 3.92 0.00 0.12 P value < 0.001 0.048 0.960 0.728 Breastfeeding duration (months) 663 Never 71 15.19 Referent 14.64 Referent Referent 12.17 Referent < 4 80 14.53 −0.67
(−1.34, 0.01)14.29 −0.34
(−0.87, 0.19)12.25 −0.04
(−0.32, 0.24)12.15 −0.01
(−0.38, 0.33)4– 24 14.71 −0.49
(−1.46, 0.49)14.59 −0.04
(−0.77, 0.69)12.22 0.08
(−0.32, 0.49)12.21 0.04
(−0.45, 0.53)6– 196 14.57 −0.62
(−1.19, −0.04)14.30 −0.33
(−0.80, 0.13)12.34 0.001
(−0.24, 0.24)12.17 −0.003
(−0.32, 0.31)12– 264 14.46 −0.73
(−1.29, −0.18)14.26 −0.37
(−0.82, 0.07)12.26 0.004
(−0.23, 0.23)12.08 −0.09
(−0.39, 0.21)≥ 24 28 14.69 −0.51
(−1.43, 0.42)14.48 −0.16
(−0.85, 0.53)12.26 0.001
(−0.38, 0.39)12.17 0.004
(−0.46, 0.47)F value 2.30 1.24 0.13 0.31 P value 0.044 0.290 0.986 0.908 Children aged 5 years Breastfeeding 658 Never 74 16.91 Referent 16.42 Referent 11.96 Referent 11.81 Referent Ever 584 16.46 −0.45
(−0.87, −0.02)16.40 −0.01
(−0.37, 0.33)11.90 −0.06
(−0.22, 0.10)11.87 0.06
(−0.15, 0.28)F value 4.24 0.01 0.55 0.36 P value 0.040 0.914 0.459 0.547 Breastfeeding duration (months) 637 Never 74 16.91 Referent 16.48 Referent 11.97 Referent 11.83 Referent < 4 72 16.79 −0.12
(−0.86, 0.61)16.52 0.04
(−0.52, 0.61)11.92 −0.05
(−0.32, 0.23)11.91 0.08
(−0.27, 0.42)4– 27 16.43 −0.48
(−1.48, 0.51)16.31 −0.16
(−0.87, 0.54)11.87 −0.09
(−0.47, 0.28)11.84 0.01
(−0.43, 0.44)6– 174 16.31 −0.61
(−1.22, 0.01)16.08 −0.40
(−0.89, 0.09)11.87 −0.10
(−0.33, 0.13)11.73 −0.11
(−0.41, 0.20)12– 268 16.45 −0.46
(−1.04, 0.12)16.64 0.16
(−0.29, 0.62)11.94 −0.03
(−0.25, 0.19)11.97 0.14
(−0.14, 0.42)≥ 24 22 16.19 −0.72
(−1.79, 0.35)16.14 −0.33
(−1.10, 0.43)11.76 −0.21
(0.61, 0.20)11.85 0.02
(−0.45, 0.49)F value 1.80 4.09 0.62 1.96 P value 0.110 0.001 0.684 0.083 Note. * Mean value after adjusting for gender. **Mean value after adjusting for gender, birthweight, survey site, nocturnal sleep time, middle-and high-level physical activity time, eating behavior score, balanced diet index score, parental BMI, and education level. HAZ was also adjusted for fat-free mass. After adjusting for potential confounders, the fat-free mass of ever breastfed infants was higher than that of never breastfed infants (12.84 kg vs. 12.52 kg, F = 5.91, P = 0.015) among children aged 3 years, and was lower (14.31 kg vs. 14.64 kg, F = 3.92, P = 0.048) among children aged 4 years. No statistically significant difference was observed between the two groups in children aged 5 years.
For children aged 3 and 4 years, there was no statistically significant difference in fat-free mass among those with different breastfeeding durations after adjusting for potential confounders.
For children aged 5 years, the overall difference in fat-free mass (F = 4.09, P = 0.001) among children with different breastfeeding durations was statistically significant. However, there were no significant differences between the different breastfeeding duration groups and the reference group (never breastfed).
-
After adjusting for potential confounders, there was no statistically significant difference in body fat indicators between the ever breastfed and never breastfed groups and the different breastfeeding duration groups for children aged 3–5 years (Tables 6–7).
Table 6. Relationship between breastfeeding status and body fat mass, and fat mass index
Variables n Body fat mass, kg Body fat mass index, kg/m2 Adjusted* Difference
(95% CI)Adjusted** Difference
(95% CI)Adjusted* Difference
(95% CI)Adjusted** Difference
(95% CI)Children aged 3 years Breastfeeding 632 Never 72 2.76 Referent 2.75 Referent 2.77 Referent 2.73 Referent Ever 560 2.71 0.98
(0.90, 1.07)2.79 1.02
(0.91, 1.12)2.72 0.98
(0.90, 1.10)2.80 1.03
(0.93, 1.13)F value 0.18 0.08 0.16 0.26 P value 0.669 0.771 0.690 0.608 Breastfeeding duration
(months)613 Never 72 2.76 Referent 2.74 Referent 2.77 Referent 2.73 Referent < 4 68 2.78 1.01
(0.86, 1.18)2.92 1.07
(0.89, 1.27)2.79 1.01
(0.87, 1.17)2.95 1.08
(0.91, 1.29)4– 16 2.86 1.03
(0.80, 1.34)2.83 1.03
(0.78, 1.37)2.72 0.98
(0.77, 1.26)2.76 1.01
(0.77, 1.33)6– 172 2.73 0.99
(0.87, 1.13)2.80 1.02
(0.88, 1.19)2.72 0.98
(0.87, 1.11)2.78 1.02
(0.88, 1.18)12– 264 2.69 0.97
(0.86, 1.10)2.73 1.00
(0.87, 1.15)2.72 0.98
(0.87, 1.11)2.77 1.01
(0.88, 1.17)≥ 24 21 2.44 0.88
(0.70, 1.11)2.67 0.97
(0.75, 1.27)2.53 0.91
(0.73, 1.14)2.83 1.04
(0.80, 1.34)F value 0.54 0.31 0.28 0.32 P value 0.750 0.909 0.926 0.899 Children aged 4 years Breastfeeding 692 Never 68 3.31 Referent 3.46 Referent 2.81 Referent 2.92 Referent Ever 624 3.16 0.95
(0.86, 1.05)3.18 0.92
(0.81, 1.04)2.77 0.98
(0.90, 1.08)2.78 0.95
(0.85, 1.07)F value 0.90 1.69 0.10 0.64 P value 0.344 0.194 0.749 0.425 Breastfeeding duration
(months)656 Never 68 3.31 Referent 3.49 Referent 2.81 Referent 2.94 Referent < 4 78 3.26 0.98
(0.83, 1.16)3.35 0.96
(0.79, 1.18)2.86 1.02
(0.87, 1.19)2.93 0.99
(0.82, 1.20)4– 24 3.11 0.94
(0.74, 1.19)3.04 0.87
(0.66, 1.14)2.71 0.96
(0.77, 1.21)2.63 0.89
(0.69, 1.15)6– 195 3.2 0.97
(0.84, 1.11)3.30 0.95
(0.79, 1.13)2.80 0.99
(0.87, 1.13)2.85 0.97
(0.82, 1.14)12– 263 3.11 0.94
(0.82, 1.08)3.13 0.9
(0.76, 1.06)2.74 0.97
(0.86, 1.11)2.75 0.94
(0.80, 1.10)≥ 24 28 3.16 0.95
(0.76, 1.19)3.11 0.89
(0.69, 1.15)2.75 0.98
(0.79, 1.21)2.74 0.93
(0.73, 1.18)F value 0.37 0.86 0.22 0.57 P value 0.869 0.507 0.952 0.724 Children aged 5 years Breastfeeding 643 Never 71 3.48 Referent 3.22 Referent 2.66 Referent 2.46 Referent Ever 572 3.42 0.98
(0.88, 1.09)3.54 1.1
(0.96, 1.26)2.65 1.00
(0.90, 1.10)2.73 1.11
(0.97, 1.26)F value 0.10 1.81 0.01 2.39 P value 0.747 0.180 0.929 0.123 Breastfeeding duration
(months)623 Never 71 3.49 Referent 3.24 Referent 2.66 Referent 2.48 Referent < 4 70 3.14 0.90
(0.75, 1.08)3.46 1.07
(0.85, 1.35)2.41 0.9
(0.76, 1.07)2.61 1.06
(0.85, 1.31)4– 26 3.83 1.10
(0.86, 1.41)4.22 1.30
(0.98, 1.73)2.96 1.11
(0.88, 1.4)3.24 1.31
(1.00, 1.72)6– 175 3.39 0.97
(0.83, 1.13)3.42 1.05
(0.86, 1.29)2.63 0.99
(0.85, 1.14)2.64 1.07
(0.88, 1.29)12– 259 3.49 1.00
(0.87, 1.16)3.55 1.09
(0.91, 1.32)2.71 1.02
(0.89, 1.17)2.74 1.11
(0.93, 1.32)≥ 24 22 3.43 0.98
(0.76, 1.28)3.73 1.15
(0.84, 1.57)2.66 1.00
(0.78, 1.28)2.89 1.17
(0.87, 1.56)F value 1.04 1.29 1.33 1.54 P value 0.391 0.269 0.251 0.175 Note. Regression analyses of body fat mass and body fat mass index were conducted after logarithmic transformation, and the antilog of the mean difference after logarithmic transformation was reported. * Mean value after adjusting for gender. **Mean value after adjusting for gender, birthweight, survey site, nocturnal sleep time, middle- and high-level physical activity time, eating behavior score, balanced diet index score, parental BMI, and education level. Table 7. Association of breastfeeding status with percentage of fat in children stratified by age
Variables n Percentage of body fat, % Adjusted* Difference (95% CI) Adjusted** Difference (95% CI) Children aged 3 years Breastfeeding 632 Never 72 18.22 Referent 18.44 Referent Ever 560 18.07 −0.15 (−1.41, 1.11) 18.55 0.11 (−1.33, 1.55) F value 0.05 0.02 P value 0.817 0.882 Breastfeeding duration (months) 613 Never 72 18.22 Referent 18.44 Referent < 4 68 18.54 0.32 (−1.86, 2.50) 19.40 0.96 (−1.56, 3.48) 4– 16 17.29 −0.93 (−4.40, 2.55) 17.49 −0.95 (−4.83, 2.94) 6– 172 17.99 −0.23 (−2.04, 1.58) 18.35 −0.09 (−2.20, 2.02) 12– 264 18.12 −0.10 (−1.82, 1.62) 18.39 −0.05 (−2.05, 1.95) ≥ 24 21 17.46 −0.76 (−3.95, 2.44) 19.13 0.69 (−3.06, 4.44) F value 0.28 0.51 P value 0.927 0.767 Children aged 4 years Breastfeeding 692 Never 68 18.87 Referent 19.10 Referent Ever 624 18.53 −0.35 (−1.69, 0.99) 18.63 −0.47 (−2.17, 1.23) F value 0.26 0.29 P value 0.611 0.590 Breastfeeding duration (months) 656 Never 68 18.87 Referent 19.23 Referent < 4 78 19.17 0.30 (−1.94, 2.54) 19.49 0.26 (−2.50, 3.02) 4– 24 17.99 −0.89 (−4.11, 2.33) 17.79 −1.43 (−5.16, 2.29) 6– 195 18.65 −0.21 (−2.11, 1.69) 19.02 −0.21 (−2.63, 2.20) 12– 263 18.29 −0.58 (−2.41, 1.25) 18.47 −0.76 (−3.06, 1.54) ≥ 24 28 18.20 −0.67 (−3.72, 2.37) 18.17 −1.06 (−4.59, 2.46) F value 0.47 0.60 P value 0.801 0.702 Children aged 5 years Breastfeeding 643 Never 71 18.35 Referent 17.07 Referent Ever 572 18.01 −0.34 (−1.82, 1.13) 18.50 1.43 (−0.50, 3.36) F value 0.21 2.13 P value 0.646 0.146 Breastfeeding duration (months) 623 Never 71 18.36 Referent 17.18 Referent < 4 70 17.06 −1.30 (−3.82, 1.21) 18.51 1.34 (−1.85, 4.52) 4– 26 19.97 1.60 (−1.85, 5.06) 21.31 4.13 (0.13, 8.13) 6– 175 17.87 −0.49 (−2.61, 1.62) 18.07 0.89 (−1.89, 3.68) 12– 259 18.10 −0.27 (−2.28, 1.74) 18.38 1.20 (−1.39, 3.80) ≥ 24 22 18.53 0.17 (−3.51, 3.85) 19.68 2.50 (−1.81, 6.81) F value 1.02 1.61 P value 0.404 0.157 Note. *Mean value after adjusting for gender. **Mean value after adjusting for gender, birthweight, survey site, nocturnal sleep time, middle- and high-level physical activity time, eating behavior score, balanced diet index score, parental BMI, and education level.
doi: 10.3967/bes2023.085
Association of Breastfeeding Duration with Body Composition in Children Aged 3–5 Years
-
Abstract:
Objective This study aimed to assess the relationship between the body composition of children aged 3–5 years and breastfeeding status and duration. Methods The study was conducted using data from the National Nutrition and Health Systematic Survey for children 0–17 years of age in China (CNHSC), a nationwide cross-sectional study. Breastfeeding information and potential confounders were collected using standardized questionnaires administered through face-to-face interviews. The body composition of preschool children was measured using bioelectrical impedance analysis. A multivariate linear regression model was used to assess the relationship between breastfeeding duration and body composition after adjusting for potential confounders. Results In total, 2,008 participants were included in the study. Of these, 89.2% were ever breastfed and the median duration of breastfeeding was 12 months (IQR 7–15 months). Among children aged 3 years, the height-for-age Z-score (HAZ) for the ever breastfed group was lower than that for never breastfed group (0.12 vs. 0.42, P = 0.043). In addition, the weight-for-age Z-score (WAZ) of the ever breastfed group was lower than that of the never breastfed group (0.31 vs. 0.65, P = 0.026), and the WAZ was lower in children aged 4 years who breastfed between 12 and 23 months than in those who never breastfed. Compared to the formula-fed children, the fat-free mass of breastfed infants was higher for children aged 3 years (12.84 kg vs. 12.52 kg, P = 0.015) and lower for those aged 4 years (14.31 kg vs. 14.64 kg, P = 0.048), but no difference was detected for children aged 5 years (16.40 kg vs. 16.42 kg, P = 0.910) after adjusting for potential confounders. No significant difference was detected in the weight-for-height Z-score (WHZ), body mass index (BMI)-for-age Z-score (BAZ), fat-free mass index, and body fat indicators in the ever breastfed and never breastfed groups and among various breastfeeding duration groups for children aged 3–5 years. Conclusion No obvious associations were detected between breastfeeding duration, BMI, and fat mass indicators. Future prospective studies should explore the relationship between breastfeeding status and fat-free mass. -
Key words:
- Breastfeeding /
- Breastfeeding duration /
- Bioelectrical impedance analysis /
- Body composition /
- Pre-school children
注释:1) AUTHORS’ CONTRIBUTIONS: -
Table 1. Characteristics of children and parents
Characteristics N (%) or Mean ± SD Children Age groups (years) 3 32.0 (642/2,008) 4 34.8 (700/2,008) 5 33.2 (666/2,008) Gender Boy 50.2 (1,008/2,008) Girl 49.8 (1,000/2,008) Residential area Urban 54.9 (1,102/2,008) Rural 45.1 (906/2,008) Region North 55.1 (1,106/2,008) South 44.9 (902/2,008) Survey site Taigu County 18.0 (362/2,008) Jichang District 17.9 (360/2,008) Yongji County 19.1 (384/2,008) Qingshan Lake District 17.6 (354/2,008) Yuelu District 19.4 (388/2,008) Longchuan County 8.0 (160/2,008) Annual household income (per capita CNY*) ˂ 10,000 4.2 (85/2,004) 10,000– 7.3 (146/2,004) ≥ 20,000 12.2 (245/2,004) Refusal to answer 41.3 (828/2,004) Unknown 34.9 (700/2,004) Birth weight (g) < 2,500 2.6 (47/1,814) 2,500–4,000 90.7 (1,645/1,814) > 4,000 6.7 (122/1,814) Gestational age pre-term 4.6 (92/1,997) Term 95.4 (1,905/1,997) Nutrition status Stunting 1.1 (22/2,008) Wasting 1.7 (34/2,008) Underweight 0.8 (17/2,008) Overweight or obesity 9.6 (193/2,008) Breastfeeding Never 10.8 (217/2,007) Ever 89.2 (1,790/2,007) Duration of breastfeeding (months) Never 11.2 (217/1,931) < 4 11.4 (221/1,931) 4– 3.6 (69/1,931) 6– 28.4 (548/1,931) 12– 41.4 (800/1,931) ≥ 24 3.9 (76/1,931) Middle/high-level physical activity time (min) ≥ 60 81.0 (1,467/1,811) < 60 19.0 (344/1,811) Nocturnal sleep time (hours) 9.5 ± 0.6 Eating behavior scores 19.2 ± 3.4 Diet quality distance (scores) 22.6 ± 8.9 Parents Maternal BMI (kg/m2) Underweight 9.4 (180/1,912) Normal 72.2 (1,381/1,912) Overweight 15.1 (288/1,912) Obesity 3.3 (63/1,912) Maternal education Junior high school or below 27.0 (537/1,988) High school 18.5 (368/1,988) Associate’s degree 22.6 (450/1,988) Bachelor’s degree or above 31.8 (633/1,988) Father’s BMI (kg/m2) Underweight 3.0 (56/1,868) Normal 56.6 (1,057/1,868) Overweight 33.3 (622/1,868) Obesity 7.1 (133/1,868) Father’s education Junior high school or below 29.1 (580/1,996) High school 19.5 (390/1,996) Associate’s degree 19.9 (397/1,996) Bachelor’s degree or above 31.5 (629/1,996) Note. *CNY: Chinese Yuan. Table 2. Status of body composition and anthropometric measurements for children aged 3 to 5 years
Variables Total 3 years 4 years 5 years n M (IQR) or
Mean ± SDn M (IQR) or
Mean ± SDn M (IQR) or
Mean ± SDn M (IQR) or
Mean ± SDBody composition Total body water (kg) 2,007 10.8 ± 1.7 641 9.5 ± 1.1 700 10.7 ± 1.3 666 12.2 ± 1.5 Total fat-free mass (kg) 1,999 14.6 ± 2.3 641 12.8 ± 1.5 700 14.6 ± 1.7 658 16.5 ± 1.9 Fat-free mass index (kg/m2.5) 1,999 12.3 ± 0.8 641 12.8 ± 0.8 700 12.3 ± 0.7 658 11.9 ± 0.7 Total body fat mass (kg) 1,968 3.0 (2.4, 4.0) 632 2.7 (2.1, 3.5) 693 3.2 (2.5, 4.0) 643 3.3 (2.6, 4.5) Fat mass index (kg/m2) 1,968 2.7 (2.1, 3.5) 632 2.7 (2.2, 3.4) 693 2.8 (2.2, 3.5) 643 2.6 (2.1, 3.5) Percentage body fat (%) 1,968 18.2 ± 5.5 632 18.1 ± 5.1 693 18.6 ± 5.5 643 18.1 ± 6.0 Anthropometric status* Height (cm) 2,008 107.1 ± 7.4 642 99.9 ± 4.6 700 107.1 ± 4.9 666 114.0 ± 4.9 Weight (kg) 2,002 17.4 (15.6, 19.6) 642 15.3 (14.2, 16.6) 700 17.5 (16.0, 19.3) 660 19.6 (18.0, 22.1) BMI (kg/m2) 2,002 15.4 (14.6, 16.3) 642 15.4 (14.7, 16.2) 700 15.4 (14.6, 16.3) 660 15.3 (14.5, 16.5) WAZ 2,002 0.2 ± 1.1 642 0.1 ± 1.0 700 0.2 ± 1.0 660 0.3 ± 1.2 HAZ 2,008 0.2 ± 1.0 642 0.1 ± 1.0 700 0.1 ± 1.0 666 0.3 ± 1.0 WHZ 1,342 0.1 ± 1.0 642 0.1 ±1.0 700 0.1 ± 1.0 / / BAZ 2,002 0.1 ± 1.1 642 0.1 ±1.0 700 0.2 ± 1.0 660 0.2 ± 1.3 Note. *HAZ: Height-for-age Z-score, WAZ: Weight-for-age Z-score, WHZ: Weight-for-height Z-score, BAZ: BMI-for-age Z-score. Table 3. Relationship between breastfeeding duration and HAZ and WAZ of participants stratified by age
Variables n Height-for-age Z-score (HAZ) Weight-for-age Z-score (WAZ) Adjusted* Difference
(95% CI)Adjusted** Difference
(95% CI)Adjusted* Difference
(95% CI)Adjusted** Difference
(95% CI)Children aged 3 years Breastfeeding 642 Never 72 0.27 Referent 0.42 Referent 0.15 Referent 0.25 Referent Ever 570 0.12 −0.15
(−0.41, 0.10)0.12 −0.30
(−0.58, −0.01)0.14 0
(−0.25, 0.24)0.21 −0.03
(−0.30, 0.23)F value 1.46 4.14 0 0.06 P value 0.228 0.043 0.969 0.799 Breastfeeding duration (months) 623 Never 72 0.27 Referent 0.41 Referent 0.15 Referent 0.24 Referent < 4 68 0 −0.27
(−0.71, 0.16)−0.03 −0.43
(−0.94, 0.08)0.13 −0.02
(−0.44, 0.40)0.27 0.03
(−0.44, 0.49)4– 17 0.66 0.39
(−0.31, 1.08)0.38 −0.02
(−0.81, 0.76)0.50 0.36
(−0.32, 1.03)0.32 0.07
(−0.65, 0.79)6– 174 0.21 −0.06
(−0.42, 0.30)0.23 −0.18
(−0.60, 0.25)0.18 0.03
(−0.32, 0.38)0.24 0
(−0.39, 0.39)12– 266 0.03 −0.24
(−0.59, 0.10)0.05 −0.36
(−0.76, 0.05)0.07 −0.07
(−0.40, 0.26)0.19 −0.06
(−0.43, 0.31)≥ 24 26 0.16 −0.12
(−0.70, 0.47)−0.29 −0.69
(−1.41, 0.03)0.34 0.20
(−0.37, 0.77)−0.08 −0.33
(−0.99, 0.34)F value 2.15 2.19 0.96 0.44 P value 0.058 0.054 0.440 0.819 Children aged 4 years Breastfeeding 699 Never 71 0.38 Referent 0.40 Referent 0.46 Referent 0.65 Referent Ever 628 0.10 −0.28
(−0.51, −0.04)0.12 −0.28
(−0.58, 0.01)0.15 −0.31
(−0.55, −0.07)0.31 −0.34
(−0.64, −0.04)F value 5.21 3.60 6.22 4.99 P value 0.023 0.058 0.013 0.026 Breastfeeding duration (months) 663 Never 71 0.38 Referent 0.41 Referent 0.46 Referent 0.66 Referent < 4 80 0.21 −0.17
(−0.56, 0.22)0.29 −0.12
(−0.60, 0.35)0.21 −0.25
(−0.65, 0.15)0.44 −0.22
(−0.70, 0.26)4– 24 0.10 −0.28
(−0.85, 0.29)0.14 −0.28
(−0.93, 0.37)0.15 −0.31
(−0.89, 0.28)0.29 −0.37
(−1.03, 0.29)6– 196 0.16 −0.22
(−0.55, 0.12)0.23 −0.18
(−0.60, 0.24)0.20 −0.26
(−0.60, 0.08)0.48 −0.18
(−0.61, 0.24)12– 264 0.07 −0.30
(−0.62, 0.02)0.03 −0.38
(−0.78, 0.02)0.15 −0.31
(−0.64, 0.02)0.21 −0.45
(−0.85, −0.05)≥ 24 28 0.10 −0.27
(−0.81, 0.26)−0.06 −0.47
(−1.09, 0.14)0.13 −0.33
(−0.88, 0.22)0.14 −0.52
(−1.15, 0.10)F value 1.23 1.84 1.18 2.43 P value 0.292 0.105 0.315 0.034 Children aged 5 years Breastfeeding 666 Never 74 0.48 Referent 0.40 Referent 0.53 Referent 0.33 Referent Ever 592 0.32 −0.16
(−0.39, 0.06)0.34 −0.06
(−0.35, 0.24)0.13 −0.22
(−0.49, 0.06)0.34 0.02
(−0.34, 0.37)F value 1.96 0.14 2.46 0.01 P value 0.162 0.709 0.117 0.925 Breastfeeding duration (months) 645 Never 74 0.48 Referent 0.40 Referent 0.53 Referent 0.36 Referent < 4 73 0.51 0.03
(−0.36, 0.42)0.67 0.27
(−0.22, 0.75)0.37 −0.16
(−0.63, 0.31)0.64 0.28
(−0.30, 0.87)4– 28 0.42 0.06
(−0.59, 0.46)0.42 0.01
(−0.60, 0.63)0.53 0
(−0.64, 0.63)0.68 0.32
(−0.42, 1.06)6– 178 0.35 −0.13
(−0.46, 0.19)0.39 −0.01
(−0.44, 0.41)0.28 −0.26
(−0.65, 0.14)0.25 −0.10
(−0.62, 0.41)12– 270 0.22 −0.27
(−0.58, 0.04)0.25 −0.15
(−0.55, 0.24)0.29 −0.25
(−0.62, 0.13)0.34 −0.02
(−0.49, 0.46)≥ 24 22 0.41 −0.07
(−0.65, 0.50)0.39 −0.01
(−0.67, 0.65)0.16 −0.37
(−1.07, 0.32)0.23 −0.13
(−0.92, 0.67)F value 1.82 1.69 0.92 1.28 P value 0.106 0.135 0.470 0.273 Note. *Mean value after adjusting for gender. **Mean values after adjusting for gender, birth weight, survey site, nocturnal sleep time, middle- and high-level physical activity time, eating behavior score, balanced diet index score, parental BMI, and education level. Table 4. Relationship between breastfeeding duration and WHZ or BAZ of participants stratified by age
Variables n Weight-for-height Z-score (WHZ) or BMI-for-age Z-score (BAZ) Adjusted* Difference (95% CI) Adjusted** Difference (95% CI) Children aged 3 years Breastfeeding 642 Never 72 −0.02 Referent 0.001 Referent Ever 570 0.11 −0.08 0.21 0.21 (−0.07, 0.49) F value 1.00 0.13 (−0.12, 0.37) 2.08 P value 0.317 0.150 Breastfeeding duration (months) 623 Never 72 −0.02 Referent 0.01 Referent < 4 68 0.19 0.21 (−0.22, 0.64) 0.42 0.42 (−0.08, 0.91) 4– 17 0.19 0.21 (−0.48, 0.89) 0.14 0.13 (−0.63, 0.89) 6– 174 0.08 0.10 (−0.26, 0.45) 0.16 0.15 (−0.26, 0.56) 12– 266 0.09 0.37 (−0.21, 0.95) 0.22 0.22 (−0.17, 0.61) ≥ 24 26 0.35 0.10 0.09 (−0.61, 0.80) F value 0.70 1.03 P value 0.626 0.399 Children aged 4 years Breastfeeding 699 Never 71 0.28 Referent 0.55 Referent Ever 628 0.10 −0.18 (−0.43, 0.07) 0.32 −0.23 (−0.54, 0.09) F value 2.09 1.94 P value 0.149 0.164 Breastfeeding duration (months) 663 Never 71 0.28 Referent 0.56 Referent < 4 80 0.10 −0.19 (−0.60, 0.23) 0.37 −0.20 (−0.71, 0.32) 4– 24 0.09 −0.19 (−0.80, 0.41) 0.27 −0.29 (−1.00, 0.42) 6– 196 0.12 −0.17 (−0.52, 0.19) 0.47 −0.09 (−0.54, 0.37) 12– 264 0.12 −0.16 (−0.51, 0.18) 0.26 −0.30 (−0.74, 0.13) ≥ 24 28 0.05 −0.23 (−0.81, 0.34) 0.22 −0.34 (−1.01, 0.33) F value 0.39 1.10 P value 0.856 0.361 Children aged 5 years Breastfeeding 666 Never 74 0.31 Referent 0.08 Referent Ever 592 0.14 −0.17 (−0.47, 0.13) 0.17 0.09 (−0.30, 0.49) F value 1.26 0.21 P value 0.262 0.649 Breastfeeding duration (months) 645 Never 74 0.32 Referent 0.12 Referent < 4 73 0.08 −0.24 (−0.76, 0.27) 0.33 0.21 (−0.44, 0.86) 4– 28 0.40 0.08 (−0.61, 0.77) 0.62 0.50 (−0.32, 1.33) 6– 178 0.07 −0.25 (−0.68, 0.18) −0.01 −0.13 (−0.70, 0.44) 12– 270 0.20 −0.12 (−0.53, 0.29) 0.25 0.13 (−0.40, 0.66) ≥ 24 22 −0.23 −0.55 (−1.30, 0.21) −0.12 −0.24 (−1.13, 0.64) F value 1.21 1.58 P value 0.301 0.166 Note. * Mean value after adjusting for gender. **Mean value after adjusting for gender, birthweight, survey site, nocturnal sleep time, middle- and high-level physical activity time, eating behavior score, balanced diet index score, parental BMI, and education level. Table 5. Relationship between breastfeeding status and fat-free mass and fat-free mass index of children stratified by age
Variables n Fat-free mass, kg Fat-free mass index, kg/m2.5 Adjusted* Difference
(95% CI)Adjusted** Difference
(95% CI)Adjusted* Difference
(95% CI)Adjusted** Difference
(95% CI)Children aged 3 years Breastfeeding 641 Never 71 12.72 Referent 12.52 Referent 12.70 Referent 12.62 Referent Ever 570 12.75 0.03
(−0.32, 0.38)12.84 0.32
(0.06, 0.59)12.75 0.05
(−0.14, 0.24)12.76 0.14
(−0.09, 0.37)F value 0.03 5.91 0.31 1.50 P value 0.868 0.015 0.580 0.222 Breastfeeding duration (months) 622 Never 71 12.72 Referent 12.51 Referent 12.70 Referent 12.63 Referent < 4 68 12.76 0.03
(−0.57, 0.64)12.98 0.46
(0.01, 0.92)12.74 0.04
(−0.29, 0.37)12.83 0.21
(−0.19, 0.61)4– 17 13.62 0.89
(−0.07, 1.86)13.06 0.54
(−0.16, 1.24)12.74 0.04
(−0.49, 0.56)12.70 0.07
(−0.54, 0.69)6– 174 12.85 0.13
(−0.37, 0.63)12.85 0.33
(−0.05, 0.71)12.74 0.04
(−0.23, 0.31)12.74 0.11
(−0.22, 0.44)12– 266 12.63 −0.10
(−0.58, 0.38)12.85 0.33
(−0.03, 0.69)12.77 0.07
(−0.18, 0.33)12.81 0.19
(−0.13, 0.51)≥ 24 26 12.76 0.03
(−0.78, 0.85)12.57 0.06
(−0.59, 0.71)12.77 0.08
(−0.37, 0.52)12.74 0.12
(−0.45, 0.68)F value 1.88 1.94 0.13 0.59 P value 0.096 0.086 0.986 0.711 Children aged 4 years Breastfeeding 699 Never 71 15.20 Referent 14.64 Referent 12.25 Referent 12.17 Referent Ever 628 14.49 −0.71
(−1.12, −0.30)14.31 −0.33
(−0.66, −0.003)12.25 −0.004
(−0.17, 0.16)12.13 −0.04
(−0.26, 0.18)F value 11.79 3.92 0.00 0.12 P value < 0.001 0.048 0.960 0.728 Breastfeeding duration (months) 663 Never 71 15.19 Referent 14.64 Referent Referent 12.17 Referent < 4 80 14.53 −0.67
(−1.34, 0.01)14.29 −0.34
(−0.87, 0.19)12.25 −0.04
(−0.32, 0.24)12.15 −0.01
(−0.38, 0.33)4– 24 14.71 −0.49
(−1.46, 0.49)14.59 −0.04
(−0.77, 0.69)12.22 0.08
(−0.32, 0.49)12.21 0.04
(−0.45, 0.53)6– 196 14.57 −0.62
(−1.19, −0.04)14.30 −0.33
(−0.80, 0.13)12.34 0.001
(−0.24, 0.24)12.17 −0.003
(−0.32, 0.31)12– 264 14.46 −0.73
(−1.29, −0.18)14.26 −0.37
(−0.82, 0.07)12.26 0.004
(−0.23, 0.23)12.08 −0.09
(−0.39, 0.21)≥ 24 28 14.69 −0.51
(−1.43, 0.42)14.48 −0.16
(−0.85, 0.53)12.26 0.001
(−0.38, 0.39)12.17 0.004
(−0.46, 0.47)F value 2.30 1.24 0.13 0.31 P value 0.044 0.290 0.986 0.908 Children aged 5 years Breastfeeding 658 Never 74 16.91 Referent 16.42 Referent 11.96 Referent 11.81 Referent Ever 584 16.46 −0.45
(−0.87, −0.02)16.40 −0.01
(−0.37, 0.33)11.90 −0.06
(−0.22, 0.10)11.87 0.06
(−0.15, 0.28)F value 4.24 0.01 0.55 0.36 P value 0.040 0.914 0.459 0.547 Breastfeeding duration (months) 637 Never 74 16.91 Referent 16.48 Referent 11.97 Referent 11.83 Referent < 4 72 16.79 −0.12
(−0.86, 0.61)16.52 0.04
(−0.52, 0.61)11.92 −0.05
(−0.32, 0.23)11.91 0.08
(−0.27, 0.42)4– 27 16.43 −0.48
(−1.48, 0.51)16.31 −0.16
(−0.87, 0.54)11.87 −0.09
(−0.47, 0.28)11.84 0.01
(−0.43, 0.44)6– 174 16.31 −0.61
(−1.22, 0.01)16.08 −0.40
(−0.89, 0.09)11.87 −0.10
(−0.33, 0.13)11.73 −0.11
(−0.41, 0.20)12– 268 16.45 −0.46
(−1.04, 0.12)16.64 0.16
(−0.29, 0.62)11.94 −0.03
(−0.25, 0.19)11.97 0.14
(−0.14, 0.42)≥ 24 22 16.19 −0.72
(−1.79, 0.35)16.14 −0.33
(−1.10, 0.43)11.76 −0.21
(0.61, 0.20)11.85 0.02
(−0.45, 0.49)F value 1.80 4.09 0.62 1.96 P value 0.110 0.001 0.684 0.083 Note. * Mean value after adjusting for gender. **Mean value after adjusting for gender, birthweight, survey site, nocturnal sleep time, middle-and high-level physical activity time, eating behavior score, balanced diet index score, parental BMI, and education level. HAZ was also adjusted for fat-free mass. Table 6. Relationship between breastfeeding status and body fat mass, and fat mass index
Variables n Body fat mass, kg Body fat mass index, kg/m2 Adjusted* Difference
(95% CI)Adjusted** Difference
(95% CI)Adjusted* Difference
(95% CI)Adjusted** Difference
(95% CI)Children aged 3 years Breastfeeding 632 Never 72 2.76 Referent 2.75 Referent 2.77 Referent 2.73 Referent Ever 560 2.71 0.98
(0.90, 1.07)2.79 1.02
(0.91, 1.12)2.72 0.98
(0.90, 1.10)2.80 1.03
(0.93, 1.13)F value 0.18 0.08 0.16 0.26 P value 0.669 0.771 0.690 0.608 Breastfeeding duration
(months)613 Never 72 2.76 Referent 2.74 Referent 2.77 Referent 2.73 Referent < 4 68 2.78 1.01
(0.86, 1.18)2.92 1.07
(0.89, 1.27)2.79 1.01
(0.87, 1.17)2.95 1.08
(0.91, 1.29)4– 16 2.86 1.03
(0.80, 1.34)2.83 1.03
(0.78, 1.37)2.72 0.98
(0.77, 1.26)2.76 1.01
(0.77, 1.33)6– 172 2.73 0.99
(0.87, 1.13)2.80 1.02
(0.88, 1.19)2.72 0.98
(0.87, 1.11)2.78 1.02
(0.88, 1.18)12– 264 2.69 0.97
(0.86, 1.10)2.73 1.00
(0.87, 1.15)2.72 0.98
(0.87, 1.11)2.77 1.01
(0.88, 1.17)≥ 24 21 2.44 0.88
(0.70, 1.11)2.67 0.97
(0.75, 1.27)2.53 0.91
(0.73, 1.14)2.83 1.04
(0.80, 1.34)F value 0.54 0.31 0.28 0.32 P value 0.750 0.909 0.926 0.899 Children aged 4 years Breastfeeding 692 Never 68 3.31 Referent 3.46 Referent 2.81 Referent 2.92 Referent Ever 624 3.16 0.95
(0.86, 1.05)3.18 0.92
(0.81, 1.04)2.77 0.98
(0.90, 1.08)2.78 0.95
(0.85, 1.07)F value 0.90 1.69 0.10 0.64 P value 0.344 0.194 0.749 0.425 Breastfeeding duration
(months)656 Never 68 3.31 Referent 3.49 Referent 2.81 Referent 2.94 Referent < 4 78 3.26 0.98
(0.83, 1.16)3.35 0.96
(0.79, 1.18)2.86 1.02
(0.87, 1.19)2.93 0.99
(0.82, 1.20)4– 24 3.11 0.94
(0.74, 1.19)3.04 0.87
(0.66, 1.14)2.71 0.96
(0.77, 1.21)2.63 0.89
(0.69, 1.15)6– 195 3.2 0.97
(0.84, 1.11)3.30 0.95
(0.79, 1.13)2.80 0.99
(0.87, 1.13)2.85 0.97
(0.82, 1.14)12– 263 3.11 0.94
(0.82, 1.08)3.13 0.9
(0.76, 1.06)2.74 0.97
(0.86, 1.11)2.75 0.94
(0.80, 1.10)≥ 24 28 3.16 0.95
(0.76, 1.19)3.11 0.89
(0.69, 1.15)2.75 0.98
(0.79, 1.21)2.74 0.93
(0.73, 1.18)F value 0.37 0.86 0.22 0.57 P value 0.869 0.507 0.952 0.724 Children aged 5 years Breastfeeding 643 Never 71 3.48 Referent 3.22 Referent 2.66 Referent 2.46 Referent Ever 572 3.42 0.98
(0.88, 1.09)3.54 1.1
(0.96, 1.26)2.65 1.00
(0.90, 1.10)2.73 1.11
(0.97, 1.26)F value 0.10 1.81 0.01 2.39 P value 0.747 0.180 0.929 0.123 Breastfeeding duration
(months)623 Never 71 3.49 Referent 3.24 Referent 2.66 Referent 2.48 Referent < 4 70 3.14 0.90
(0.75, 1.08)3.46 1.07
(0.85, 1.35)2.41 0.9
(0.76, 1.07)2.61 1.06
(0.85, 1.31)4– 26 3.83 1.10
(0.86, 1.41)4.22 1.30
(0.98, 1.73)2.96 1.11
(0.88, 1.4)3.24 1.31
(1.00, 1.72)6– 175 3.39 0.97
(0.83, 1.13)3.42 1.05
(0.86, 1.29)2.63 0.99
(0.85, 1.14)2.64 1.07
(0.88, 1.29)12– 259 3.49 1.00
(0.87, 1.16)3.55 1.09
(0.91, 1.32)2.71 1.02
(0.89, 1.17)2.74 1.11
(0.93, 1.32)≥ 24 22 3.43 0.98
(0.76, 1.28)3.73 1.15
(0.84, 1.57)2.66 1.00
(0.78, 1.28)2.89 1.17
(0.87, 1.56)F value 1.04 1.29 1.33 1.54 P value 0.391 0.269 0.251 0.175 Note. Regression analyses of body fat mass and body fat mass index were conducted after logarithmic transformation, and the antilog of the mean difference after logarithmic transformation was reported. * Mean value after adjusting for gender. **Mean value after adjusting for gender, birthweight, survey site, nocturnal sleep time, middle- and high-level physical activity time, eating behavior score, balanced diet index score, parental BMI, and education level. Table 7. Association of breastfeeding status with percentage of fat in children stratified by age
Variables n Percentage of body fat, % Adjusted* Difference (95% CI) Adjusted** Difference (95% CI) Children aged 3 years Breastfeeding 632 Never 72 18.22 Referent 18.44 Referent Ever 560 18.07 −0.15 (−1.41, 1.11) 18.55 0.11 (−1.33, 1.55) F value 0.05 0.02 P value 0.817 0.882 Breastfeeding duration (months) 613 Never 72 18.22 Referent 18.44 Referent < 4 68 18.54 0.32 (−1.86, 2.50) 19.40 0.96 (−1.56, 3.48) 4– 16 17.29 −0.93 (−4.40, 2.55) 17.49 −0.95 (−4.83, 2.94) 6– 172 17.99 −0.23 (−2.04, 1.58) 18.35 −0.09 (−2.20, 2.02) 12– 264 18.12 −0.10 (−1.82, 1.62) 18.39 −0.05 (−2.05, 1.95) ≥ 24 21 17.46 −0.76 (−3.95, 2.44) 19.13 0.69 (−3.06, 4.44) F value 0.28 0.51 P value 0.927 0.767 Children aged 4 years Breastfeeding 692 Never 68 18.87 Referent 19.10 Referent Ever 624 18.53 −0.35 (−1.69, 0.99) 18.63 −0.47 (−2.17, 1.23) F value 0.26 0.29 P value 0.611 0.590 Breastfeeding duration (months) 656 Never 68 18.87 Referent 19.23 Referent < 4 78 19.17 0.30 (−1.94, 2.54) 19.49 0.26 (−2.50, 3.02) 4– 24 17.99 −0.89 (−4.11, 2.33) 17.79 −1.43 (−5.16, 2.29) 6– 195 18.65 −0.21 (−2.11, 1.69) 19.02 −0.21 (−2.63, 2.20) 12– 263 18.29 −0.58 (−2.41, 1.25) 18.47 −0.76 (−3.06, 1.54) ≥ 24 28 18.20 −0.67 (−3.72, 2.37) 18.17 −1.06 (−4.59, 2.46) F value 0.47 0.60 P value 0.801 0.702 Children aged 5 years Breastfeeding 643 Never 71 18.35 Referent 17.07 Referent Ever 572 18.01 −0.34 (−1.82, 1.13) 18.50 1.43 (−0.50, 3.36) F value 0.21 2.13 P value 0.646 0.146 Breastfeeding duration (months) 623 Never 71 18.36 Referent 17.18 Referent < 4 70 17.06 −1.30 (−3.82, 1.21) 18.51 1.34 (−1.85, 4.52) 4– 26 19.97 1.60 (−1.85, 5.06) 21.31 4.13 (0.13, 8.13) 6– 175 17.87 −0.49 (−2.61, 1.62) 18.07 0.89 (−1.89, 3.68) 12– 259 18.10 −0.27 (−2.28, 1.74) 18.38 1.20 (−1.39, 3.80) ≥ 24 22 18.53 0.17 (−3.51, 3.85) 19.68 2.50 (−1.81, 6.81) F value 1.02 1.61 P value 0.404 0.157 Note. *Mean value after adjusting for gender. **Mean value after adjusting for gender, birthweight, survey site, nocturnal sleep time, middle- and high-level physical activity time, eating behavior score, balanced diet index score, parental BMI, and education level. -
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