2017 Vol. 30, No. 9

2017-09cover
2017, 30(9): 1-1.
2017-09contents
2017, 30(9): 2-2.
Original Articles
Antioxidant and Anti-aging Activities of Silybum Marianum Protein Hydrolysate in Mice Treated with D-galactose
ZHU Shu Yun, JIANG Ning, TU Jie, YANG Jing, ZHOU Yue
2017, 30(9): 623-631. doi: 10.3967/bes2017.083
Objective In the present study, we investigated the antioxidant and anti-aging effects of Silybum marianum protein hydrolysate (SMPH) in D-galactose-treated mice. Methods D-galactose (500 mg/kg body weight) was intraperitoneally injected daily for 7 weeks to accelerate aging, and SMPH (400, 800, 1, 200 mg/kg body weight, respectively) was simultaneously administered orally. The antioxidant and anti-aging effects of SMPH in the liver and brain were measured by biochemical assays. Transmission electron microscopy (TEM) was performed to study the ultrastructure of liver mitochondria. Results SMPH decreased triglyceride and cholesterol levels in the D-galactose-treated mice. It significantly elevated the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC), which were suppressed by D-galactose. Monoamine oxidase (MAO) and malondialdehyde (MDA) levels as well as the concentrations of caspase-3 and 8-OHdG in the liver and brain were significantly reduced by SMPH. Moreover, it increased Bcl-2 levels in the liver and brain. Furthermore, SMPH significantly attenuated D-galactose-induced liver mitochondrial dysfunction by improving the activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase as well as mitochondrial membrane potential (ΔΨm) and fluidity. TEM showed that the degree of liver mitochondrial damage was significantly decreased by SMPH. Conclusion The results indicated that SMPH protects against D-galactose-induced accelerated aging in mice through its antioxidant and anti-aging activities.
Combined Effects of Family History of Cardiovascular Disease and Serum C-reactive Protein Level on the Risk of Stroke:A 9.2-year Prospective Study among Mongolians in China
ZHU Zheng Bao, HUANGFU Xin Feng, ZHONG Chong Ke, ZHOU Yi Peng, TIAN Yun Fan, BUREN Batu, XU Tian, WANG Ai Li, LI Hong Mei, ZHANG Ming Zhi, ZHANG Yong Hong
2017, 30(9): 632-640. doi: 10.3967/bes2017.084