Volume 20 Issue 1
Feb.  2007
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DAN-HUI LIU, XIAO-MIN WANG, LI-JUAN ZHANG, SONG-WEI DAI, LI-YUN LIU, JI-FU LIU, SHAN-SHAN WU, SHUAN-YING YANG, SAM FU, XUE-YUAN XIAO, DA-CHENG HE. Serum Amyloid A Protein: A Potential Biomarker Correlated With Clinical Stage of Lung Cancer[J]. Biomedical and Environmental Sciences, 2007, 20(1): 33-40.
Citation: DAN-HUI LIU, XIAO-MIN WANG, LI-JUAN ZHANG, SONG-WEI DAI, LI-YUN LIU, JI-FU LIU, SHAN-SHAN WU, SHUAN-YING YANG, SAM FU, XUE-YUAN XIAO, DA-CHENG HE. Serum Amyloid A Protein: A Potential Biomarker Correlated With Clinical Stage of Lung Cancer[J]. Biomedical and Environmental Sciences, 2007, 20(1): 33-40.

Serum Amyloid A Protein: A Potential Biomarker Correlated With Clinical Stage of Lung Cancer

Funds:  国家自然科学基金(30370712)%Beijing Key Project(7051002)%Beijing Science Technology Committee Project(Y0204002040111)%Majon State Basic Research Program of China(2006CB%910100)
  • Objective To identify serum diagnosis or progression biomarkers in patients with lung cancer using protein chip profiling analysis. Method Profiling analysis was performed on 450 sera collected from 213 patients with lung cancer, 19with pneumonia, 16 with pulmonary tuberculosis, 65 with laryngeal carcinoma, 55 with laryngopharyngeal carcinoma patients,and 82 normal individuals. A new strategy was developed to identify the biomarkers on chip by trypsin pre-digestion. Results Profiling analysis demonstrated that an 11.6kDa protein was significantly elevated in lung cancer patients, compared with the control groups (P<0.001). The level and percentage of 11.6kDa protein progressively increased with the clinical stages Ⅰ-Ⅳ and were also higher in patients with squamous cell carcinoma than in other subtypes. This biomarker could be decreased after operation or chemotherapy. On the other hand, 11.6kDa protein was also increased in 50% benign diseases of lung and 13% of other cancer controls. After trypsin pre-digestion, a set of new peptide biomarkers was noticed to appear only in the samples containing a 11.6kDa peak. Further identification showed that 2177Da was a fragment of serum amyloid A (SAA, MW 11.6kDa). Two of the new peaks, 1550Da and 1611Da, were defined from the same protein by database searching. This result was further confirmed by partial purification of 11.6kDa protein and MS analysis. Conclusion SAA is a useful biomarker to monitor the progression of lung cancer and can directly identify some biomarkers on chip.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Serum Amyloid A Protein: A Potential Biomarker Correlated With Clinical Stage of Lung Cancer

Funds:  国家自然科学基金(30370712)%Beijing Key Project(7051002)%Beijing Science Technology Committee Project(Y0204002040111)%Majon State Basic Research Program of China(2006CB%910100)

Abstract: Objective To identify serum diagnosis or progression biomarkers in patients with lung cancer using protein chip profiling analysis. Method Profiling analysis was performed on 450 sera collected from 213 patients with lung cancer, 19with pneumonia, 16 with pulmonary tuberculosis, 65 with laryngeal carcinoma, 55 with laryngopharyngeal carcinoma patients,and 82 normal individuals. A new strategy was developed to identify the biomarkers on chip by trypsin pre-digestion. Results Profiling analysis demonstrated that an 11.6kDa protein was significantly elevated in lung cancer patients, compared with the control groups (P<0.001). The level and percentage of 11.6kDa protein progressively increased with the clinical stages Ⅰ-Ⅳ and were also higher in patients with squamous cell carcinoma than in other subtypes. This biomarker could be decreased after operation or chemotherapy. On the other hand, 11.6kDa protein was also increased in 50% benign diseases of lung and 13% of other cancer controls. After trypsin pre-digestion, a set of new peptide biomarkers was noticed to appear only in the samples containing a 11.6kDa peak. Further identification showed that 2177Da was a fragment of serum amyloid A (SAA, MW 11.6kDa). Two of the new peaks, 1550Da and 1611Da, were defined from the same protein by database searching. This result was further confirmed by partial purification of 11.6kDa protein and MS analysis. Conclusion SAA is a useful biomarker to monitor the progression of lung cancer and can directly identify some biomarkers on chip.

DAN-HUI LIU, XIAO-MIN WANG, LI-JUAN ZHANG, SONG-WEI DAI, LI-YUN LIU, JI-FU LIU, SHAN-SHAN WU, SHUAN-YING YANG, SAM FU, XUE-YUAN XIAO, DA-CHENG HE. Serum Amyloid A Protein: A Potential Biomarker Correlated With Clinical Stage of Lung Cancer[J]. Biomedical and Environmental Sciences, 2007, 20(1): 33-40.
Citation: DAN-HUI LIU, XIAO-MIN WANG, LI-JUAN ZHANG, SONG-WEI DAI, LI-YUN LIU, JI-FU LIU, SHAN-SHAN WU, SHUAN-YING YANG, SAM FU, XUE-YUAN XIAO, DA-CHENG HE. Serum Amyloid A Protein: A Potential Biomarker Correlated With Clinical Stage of Lung Cancer[J]. Biomedical and Environmental Sciences, 2007, 20(1): 33-40.

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