Current Articles

2024-5 Cover
2024, 37(5)
2024-5 Contents
2024, 37(5): 1-2.
Original Article
A Comprehensive Study of the Association between LEPR Gene rs1137101 Variant and Risk of Digestive System Cancers
HU Wei Qiong, ZHOU Wei Guang, ZHOU Guang Wei, LIAO Jia Xi, SHI Jia Xing, XIE FengYang, LI Shou Heng, WANG Yong, FENG Xian Hong, GU Xiu Li, CHEN Bi Feng
2024, 37(5): 445-456. doi: 10.3967/bes2024.051
  Objective   The leptin receptor, encoded by the LEPR gene, is involved in tumorigenesis. A potential functional variant of LEPR, rs1137101 (Gln223Arg), has been extensively investigated for its contribution to the risk of digestive system (DS) cancers, but results remain conflicting rather than conclusive. Here, we performed a case–control study and subsequent meta-analysis to examine the association between rs1137101 and DS cancer risk.  Methods   A total of 1,727 patients with cancer (gastric/liver/colorectal: 460/480/787) and 800 healthy controls were recruited. Genotyping of rs1137101 was conducted using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay and confirmed using Sanger sequencing. Twenty-four eligible studies were included in the meta-analysis.  Results   After Bonferroni correction, the case–control study revealed that rs1137101 was significantly associated with the risk of liver cancer in the Hubei Chinese population. The meta-analysis suggested that rs1137101 is significantly associated with the risk of overall DS, gastric, and liver cancer in the Chinese population.  Conclusion   The LEPR rs1137101 variant may be a genetic biomarker for susceptibility to DS cancers (especially liver and gastric cancer) in the Chinese population.
Clinicopathological Features and Long-Term Prognostic Role of Human Epidermal Growth Factor Receptor-2 Low Expression in Chinese Patients with Early Breast Cancer: A Single-Institution Study
KONG Zi Qing, LIU Li Qun, HUANG De Qin, WANG Yu Tong, LI Jing Jie, ZHANG Zheng, WANG Xi Xi, LIU Chuan Ling, ZHANG Ya Di, SHAO Jia Kang, ZHU Yi Min, CHEN Yi Meng, LIU Mei, ZHAO Wei Hong
2024, 37(5): 457-470. doi: 10.3967/bes2024.014
  Objective   This study aimed to comprehensively analyze and compare the clinicopathological features and prognosis of Chinese patients with human epidermal growth factor receptor 2 (HER2)-low early breast cancer (BC) and HER2-IHC0 BC.  Methods   Patients diagnosed with HER2-negative BC (N = 999) at our institution between January 2011 and December 2015 formed our study population. Clinicopathological characteristics, association between estrogen receptor (ER) expression and HER2-low, and evolution of HER2 immunohistochemical (IHC) score were assessed. Kaplan-Meier method and log-rank test were used to compare the long-term survival outcomes (5-year follow-up) between the HER2-IHC0 and HER2-low groups.  Results   HER2-low BC group tended to demonstrate high expression of ER and more progesterone receptor (PgR) positivity than HER2-IHC0 BC group (P < 0.001). The rate of HER2-low status increased with increasing ER expression levels (Mantel-Haenszel χ2 test, P < 0.001, Pearson’s R = 0.159, P < 0.001). Survival analysis revealed a significantly longer overall survival (OS) in HER2-low BC group than in HER2-IHC0 group (P = 0.007) in the whole cohort and the hormone receptor (HR)-negative group. There were no significant differences between the two groups in terms of disease-free survival (DFS). The discordance rate of HER2 IHC scores between primary and metastatic sites was 36.84%.  Conclusion   HER2-low BC may not be regarded as a unique BC group in this population-based study due to similar clinicopathological features and prognostic roles.
Association of Human Whole-blood NAD+ Levels with Nabothian Cyst
XU Ling, WANG Yue Xuan, WANG Wei, FAN Xue, CHEN Xue Yu, ZHOU Tian Yun, LIU Yu He, YU Ye, YANG Fan, JU Zhen Yu, ZHOU Yong, WANG Deng Liang
2024, 37(5): 471-478. doi: 10.3967/bes2024.052
  Objective  Little is known about the association between whole-blood nicotinamide adenine dinucleotide (NAD+) levels and nabothian cysts. This study aimed to assess the association between NAD+ levels and nabothian cysts in healthy Chinese women.  Methods  Multivariate logistic regression analysis was performed to analyze the association between NAD+ levels and nabothian cysts.  Results  The mean age was 43.0 ± 11.5 years, and the mean level of NAD+ was 31.3 ± 5.3 μmol/L. Nabothian cysts occurred in 184 (27.7%) participants, with single and multiple cysts in 100 (15.0%) and 84 (12.6%) participants, respectively. The total nabothian cyst prevalence gradually decreased from 37.4% to 21.6% from Q1 to Q4 of NAD+ and the prevalence of single and multiple nabothian cysts also decreased across the NAD+ quartiles. As compared with the highest NAD+ quartile (≥ 34.4 μmol/L), the adjusted odds ratios with 95% confidence interval of the NAD+ Q1 was 1.89 (1.14–3.14) for total nabothian cysts. The risk of total and single nabothian cysts linearly decreased with increasing NAD+ levels, while the risk of multiple nabothian cysts decreased more rapidly at NAD+ levels of 28.0 to 35.0 μmol/L.  Conclusion:   Low NAD+ levels were associated with an increased risk of total and multiple nabothian cysts.
Changes in the Non-targeted Metabolomic Profile of Three-year-old Toddlers with Elevated Exposure to Polycyclic Aromatic Hydrocarbons
LI Yang, LIN Dan, ZHANG Xiu Qin, JU Guang Xiu, SU Ya, ZHANG Qian, DUAN Hai Ping, YU Wei Sen, WANG Bing Ling, PANG Shu Tao
2024, 37(5): 479-493. doi: 10.3967/bes2024.053
  Objective  To investigate changes in the urinary metabolite profiles of children exposed to polycyclic aromatic hydrocarbons (PAHs) during critical brain development and explore their potential link with the intestinal microbiota.  Methods  Liquid chromatography-tandem mass spectrometry was used to determine ten hydroxyl metabolites of PAHs (OH-PAHs) in 36-month-old children. Subsequently, 37 children were categorized into low- and high-exposure groups based on the sum of the ten OH-PAHs. Ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was used to identify non-targeted metabolites in the urine samples. Furthermore, fecal flora abundance was assessed by 16S rRNA gene sequencing using Illumina MiSeq.  Results  The concentrations of 21 metabolites were significantly higher in the high exposure group than in the low exposure group (variable importance for projection > 1, P < 0.05). Most of these metabolites were positively correlated with the hydroxyl metabolites of naphthalene, fluorine, and phenanthrene (r = 0.336–0.531). The identified differential metabolites primarily belonged to pathways associated with inflammation or proinflammatory states, including amino acid, lipid, and nucleotide metabolism. Additionally, these distinct metabolites were significantly associated with specific intestinal flora abundances (r = 0.34–0.55), which were mainly involved in neurodevelopment.  Conclusion  Higher PAH exposure in young children affected metabolic homeostasis, particularly that of certain gut microbiota-derived metabolites. Further investigation is needed to explore the potential influence of PAHs on the gut microbiota and their possible association with neurodevelopmental outcomes.
Association of Cytokines with Clinical Indicators in Patients with Drug-Induced Liver Injury
CAO Wei Hua, JIANG Ting Ting, SHEN Ge, DENG Wen, WANG Shi Yu, ZHANG Zi Yu, LI Xin Xin, LU Yao, ZHANG Lu, LIU Ru Yu, CHANG Min, WU Shu Ling, GAO Yuan Jiao, HAO Hong Xiao, CHEN Xiao Xue, HU Lei Ping, XU Meng Jiao, YI Wei, XIE Yao, LI Ming Hui
2024, 37(5): 494-502. doi: 10.3967/bes2024.054
  Objective  To explore characteristics of clinical parameters and cytokines in patients with drug-induced liver injury (DILI) caused by different drugs and their correlation with clinical indicators.  Method  The study was conducted on patients who were up to Review of Uncertainties in Confidence Assessment for Medical Tests (RUCAM) scoring criteria and clinically diagnosed with DILI. Based on Chinese herbal medicine, cardiovascular drugs, non-steroidal anti-inflammatory drugs (NSAIDs), anti-infective drugs, and other drugs, patients were divided into five groups. Cytokines were measured by Luminex technology. Baseline characteristics of clinical biochemical indicators and cytokines in DILI patients and their correlation were analyzed.  Results  73 patients were enrolled. Age among five groups was statistically different (P = 0.032). Alanine aminotransferase (ALT) (P = 0.033) and aspartate aminotransferase (AST) (P = 0.007) in NSAIDs group were higher than those in chinese herbal medicine group. Interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) in patients with Chinese herbal medicine (IL-6: P < 0.001; TNF-α: P < 0.001) and cardiovascular medicine (IL-6: P = 0.020; TNF-α: P = 0.001) were lower than those in NSAIDs group. There was a positive correlation between ALT (r = 0.697, P = 0.025), AST (r = 0.721, P = 0.019), and IL-6 in NSAIDs group.  Conclusion  Older age may be more prone to DILI. Patients with NSAIDs have more severe liver damage in early stages of DILI, TNF-α and IL-6 may partake the inflammatory process of DILI.
Microdeletion on Xq27.1 in a Chinese VACTERL-Like Family with Kidney and Anal Anomalies
LI Min, ZHANG Yu Lan, ZHANG Kai Li, LI Ping Ping, LYU Yu Han, LIANG Ya Xin, YU Yue
2024, 37(5): 503-510. doi: 10.3967/bes2024.055
  Objective   VATER/VACTERL-like association is associated with adverse pregnancy outcomes. Genetic evidence of this disorder is sporadic. In this study, we aimed to provide genetic insights to improve the diagnosis of VACTERL.   Methods   We have described a Chinese family in which four members were affected by renal defects or agenesis, anal atresia, and anovaginal fistula, which is consistent with the diagnosis of a VACTERL-like association. Pedigree and genetic analyses were conducted using genome and exome sequencing.   Results   Segregation analysis revealed the presence of a recessive X-linked microdeletion in two living affected individuals, harboring a 196–380 kb microdeletion on Xq27.1, which was identified by familial exome sequencing. Genome sequencing was performed on the affected male, confirming a -196 kb microdeletion in Xq27.1, which included a 28% loss of the CDR-1 gene. Four family members were included in the co-segregation analysis, and only VACTERL-like cases with microdeletions were reported in X27.1.  Conclusion   These results suggest that the 196–380 kb microdeletion in Xq27.1 could be a possible cause of the VATER/VACTERL-like association. However, further genetic and functional analyses are required to confirm or rule out genetic background as the definitive cause of the VACTERL association.
Geographically and Temporally Weighted Regression in Assessing Dengue Fever Spread Factors in YunnanBorder Regions
ZHU Xiao Xiang, WANG Song Wang, LI Yan Fei, ZHANG Ye Wu, SU Xue Mei, ZHAO Xiao Tao
2024, 37(5): 511-520. doi: 10.3967/bes2024.056
  Objective  This study employs the Geographically and Temporally Weighted Regression (GTWR) model to assess the impact of meteorological elements and imported cases on dengue fever outbreaks, emphasizing the spatial-temporal variability of these factors in border regions.  Methods  We conducted a descriptive analysis of dengue fever’s temporal-spatial distribution in Yunnan border areas. Utilizing annual data from 2013 to 2019, with each county in the Yunnan border serving as a spatial unit, we constructed a GTWR model to investigate the determinants of dengue fever and their spatio-temporal heterogeneity in this region.  Results  The GTWR model, proving more effective than Ordinary Least Squares (OLS) analysis, identified significant spatial and temporal heterogeneity in factors influencing dengue fever’s spread along the Yunnan border. Notably, the GTWR model revealed a substantial variation in the relationship between indigenous dengue fever incidence, meteorological variables, and imported cases across different counties.  Conclusion  In the Yunnan border areas, local dengue incidence is affected by temperature, humidity, precipitation, wind speed, and imported cases, with these factors’ influence exhibiting notable spatial and temporal variation.
Letter to the Editor
Identification of Key Blood Biomarkers Linking Di (2-ethylhexyl) Phthalate and Autoimmune Diseases in Adolescents Mice
XU Xiao Mei, LI Xiao Lu, HU Die, CAI Xiao Yue, LI Qi Meng, ZHENG Yuan Zhuo, CAI Yang, QI Yin Yin, XU Tao, ZHAO Ling Li, NING Xia
2024, 37(5): 521-525. doi: 10.3967/bes2024.057
Network Pharmacology and Experimental Study of Momordicine I and Momordicine II from Bitter Melon Saponins in Inhibiting Fat Accumulation
HUANG Shi Ting, LI Shang Yuan, LI Xin Yu, ZHU Ying, ZHU Lin, ZHANG Jia Yan, ZHAO Yan Sheng, DAGLIA Maria, XIAO Xiang, BAI Juan
2024, 37(5): 526-530. doi: 10.3967/bes2024.058
Insufficient Physical Activity among Students Aged 6–17 Years in China, 2016–2017
XUE Tao Tao, GAO Xing Xing, WANG Li Min, ZHANG Xiao, ZHAO Zhen Ping, LI Chun, NYASHA Grace Mudoti, LIU Chen Yi, ZHANG Mei
2024, 37(5): 531-536. doi: 10.3967/bes2024.059
Short-term Association of Meteorological Elements on COVID-19 Pandemic in a Semi-arid City of Northwest China, 2021–2022
FENG Feng Liu, MA Yu Xia, CHENG Bo Wen, LIU Zong Rui, ZHAO Yu Han, YANG Jie
2024, 37(5): 537-542. doi: 10.3967/bes2024.060
Molecular Characterization of Coxsackievirus B1 Strains Isolated from Patients with Hand Foot and Mouth Disease in Yunnan, Southwest China
XU Dan Han, ZHANG Ming, CHEN Jun Wei, Feng Chang Zeng, LIU Yu Han, CHU Zhao Yang, LIU Wen Jing, LI Li, MA Shao Hui
2024, 37(5): 543-548. doi: 10.3967/bes2024.061
Standards of Basic Dataset of Chronic Disease Behavior Risk Factor Surveillance in Adults
2024, 37(5): 549-550. doi: 10.3967/bes2024.062
Interpretation of the Standards of Basic Dataset of Chronic Diseases Behavior Risk Factor Surveillance in Adults
ZHAO Yan Fang, ZHANG Mei, WEI Wen Qiang, SUN Ke Xin, LI Xin Hua, WANG Li Min
2024, 37(5): 551-556. doi: 10.3967/bes2022.063
The 10th China Obesity Science Conference Held in Beijing
2024, 37(5): 557-557. doi: 10.3967/bes2024.089