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2022, 35(6).  
2022, 35(6): 1-2.  
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Original Article
Exosomes from PM2.5-treated Human Bronchial Epithelial Cells Increase Lung Cancer Metastatic Potential
YU Heng Yi, GUO Hua Qi, FENG Yan, CHENG Wei, WANG Yan
2022, 35(6): 473-484.   doi: 10.3967/bes2022.066
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  Objective  Fine particulate matter (PM2.5) is an air pollutant that has become of great concern in recent years. Numerous studies have found that PM2.5 may contribute to lung cancer, but the pathogenesis has not yet been fully elucidated. In this study, we explored the roles of exosomes from bronchial epithelial cells in PM2.5-promoted lung cancer metastasis.  Methods  Exosomes were isolated from cell supernatants. An animal model of lung metastasis (established by tail vein injection of A549-luc) and in vitro studies with lung cancer cell lines were used to investigate the effects of exosomes derived from PM2.5-treated human bronchial epithelial cells (PHBE-exo).  Results  The animal experiments revealed that PHBE-exo-treated mice showed stronger luciferase activity and a larger relative metastatic region in the lungs, thus indicating that PHBE-exo promoted the metastatic potential of lung cancer. Additionally, PHBE-exo promoted the migration, invasion and epithelial-to-mesenchymal transition of lung cancer cells, in a manner mediated by activation of c-Jun N-terminal kinase.  Conclusion  These results implied that PM2.5 may promote the development of lung cancer through exosomes derived from bronchial epithelial cells, thus providing a potential interventional target for lung cancer. These findings broadened our understanding of cancer-promoting mechanisms of environmental pollutants.
MicroRNA-125b Accelerates and Promotes PML-RARa-driven Murine Acute Promyelocytic Leukemia
GUO Bo, QIN Ran, CHEN Ji Jun, PAN Wen, LU Xue Chun
2022, 35(6): 485-493.   doi: 10.3967/bes2022.067
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  Objective  Most acute promyelocytic leukemia cases are characterized by the PML-RARa fusion oncogene and low white cell counts in peripheral blood.  Methods  Based on the frequent overexpression of miR-125-family miRNAs in acute promyelocytic leukemia, we examined the consequence of this phenomenon by using an inducible mouse model overexpressing human miR-125b.  Results  MiR-125b expression significantly accelerates PML-RARa-induced leukemogenesis, with the resultant induced leukemia being partially dependent on continued miR-125b overexpression. Interestingly, miR-125b expression led to low peripheral white cell counts to bone marrow blast percentage ratio, confirming the clinical observation in acute promyelocytic leukemia patients.  Conclusion  This study suggests that dysregulated miR-125b expression is actively involved in disease progression and pathophysiology of acute promyelocytic leukemia, indicating that targeting miR-125b may represent a new therapeutic option for acute promyelocytic leukemia.
A Novel Early Warning Model for Hand, Foot and Mouth Disease Prediction Based on a Graph Convolutional Network
JI Tian Jiao, CHENG Qiang, ZHANG Yong, ZENG Han Ri, WANG Jian Xing, YANG Guan Yu, XU Wen Bo, LIU Hong Tu
2022, 35(6): 494-503.   doi: 10.3967/bes2022.065
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  Objectives  Hand, foot and mouth disease (HFMD) is a widespread infectious disease that causes a significant disease burden on society. To achieve early intervention and to prevent outbreaks of disease, we propose a novel warning model that can accurately predict the incidence of HFMD.  Methods  We propose a spatial-temporal graph convolutional network (STGCN) that combines spatial factors for surrounding cities with historical incidence over a certain time period to predict the future occurrence of HFMD in Guangdong and Shandong between 2011 and 2019. The 2011–2018 data served as the training and verification set, while data from 2019 served as the prediction set. Six important parameters were selected and verified in this model and the deviation was displayed by the root mean square error and the mean absolute error.  Results  As the first application using a STGCN for disease forecasting, we succeeded in accurately predicting the incidence of HFMD over a 12-week period at the prefecture level, especially for cities of significant concern.  Conclusions  This model provides a novel approach for infectious disease prediction and may help health administrative departments implement effective control measures up to 3 months in advance, which may significantly reduce the morbidity associated with HFMD in the future.
20-Hydroxyecdysone Improves Neuronal Differentiation of Adult Hippocampal Neural Stem Cells in High Power Microwave Radiation-Exposed Rats
LIU Jing Jing, ZHANG Hong Yan, CHEN Xin, ZHANG Guang Bin, LIN Jiang Kai, FENG Hua, CHU Wei Hua
2022, 35(6): 504-517.   doi: 10.3967/bes2022.068
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  Objective  The hippocampus is thought to be a vulnerable target of microwave exposure. The aim of the present study was to investigate whether 20-hydroxyecdysone (20E) acted as a fate regulator of adult rat hippocampal neural stem cells (NSCs). Furthermore, we investigated if 20E attenuated high power microwave (HMP) radiation-induced learning and memory deficits.   Methods  Sixty male Sprague-Dawley rats were randomly divided into three groups: normal controls, radiation treated, and radiation+20E treated. Rats in the radiation and radiation+20E treatment groups were exposed to HPM radiation from a microwave emission system. The learning and memory abilities of the rats were assessed using the Morris water maze test. Primary adult rat hippocampal NSCs were isolated in vitro and cultured to evaluate their proliferation and differentiation. In addition, hematoxylin & eosin staining, western blotting, and immunofluorescence were used to detect changes in the rat brain and the proliferation and differentiation of the adult rat hippocampal NSCs after HPM radiation exposure.   Results  The results showed that 20E induced neuronal differentiation of adult hippocampal NSCs from HPM radiation-exposed rats via the Wnt3a/β-catenin signaling pathway in vitro. Furthermore, 20E facilitated neurogenesis in the subgranular zone of the rat brain following HPM radiation exposure. Administration of 20E attenuated learning and memory deficits in HPM radiation-exposed rats and frizzled-related protein (FRZB) reduced the 20E-induced nuclear translocation of β-catenin, while FRZB treatment also reversed 20E-induced neuronal differentiation of NSCs in vitro.   Conclusion  These results suggested that 20E was a fate regulator of adult rat hippocampal NSCs, where it played a role in attenuating HPM radiation-induced learning and memory deficits.
Visual Detection of Vibrio parahaemolyticus using Combined CRISPR/Cas12a and Recombinase Polymerase Amplification
JIANG Han Ji, TAN Rong, JIN Min, YIN Jing, GAO Zhi Xian, LI Hai Bei, SHI Dan Yang, ZHOU Shu Qing, CHEN Tian Jiao, YANG Dong, LI Jun Wen
2022, 35(6): 518-527.   doi: 10.3967/bes2022.069
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  Objective  To establish an ultra-sensitive, ultra-fast, visible detection method for Vibrio parahaemolyticus (VP).   Methods  We established a new method for detecting the tdh and trh genes of VP using clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 12a (CRISPR/Cas12a) combined with recombinase polymerase amplification and visual detection (CRISPR/Cas12a-VD).   Results  CRISPR/Cas12a-VD accurately detected target DNA at concentrations as low as 10-18 M (single molecule detection) within 30 min without cross-reactivity against other bacteria. When detecting pure cultures of VP, the consistency of results reached 100% compared with real-time PCR. The method accurately analysed pure cultures and spiked shrimp samples at concentrations as low as 102 CFU/g.   Conclusion  The novel CRISPR/Cas12a-VD method for detecting VP performed better than traditional detection methods, such as real-time PCR, and has great potential for preventing the spread of pathogens.
Using 16S rDNA Sequencing Technology to Preliminarily Analyze Intestinal Flora in Children with Mycoplasma pneumoniae Pneumonia
SHI Da Wei, WANG Dong Mei, NING Li Hua, LI Jing, DONG Yan, ZHANG Zhi Kun, DOU Hai Wei, WAN Rui Jie, JIA Chun Mei, XIN De LI
2022, 35(6): 528-537.   doi: 10.3967/bes2022.070
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  Objective  We investigated changes in the intestinal flora of children with Mycoplasma pneumoniae pneumonia (MPP).   Methods  Between September 2019 and November 2019, stool samples from 14 children with MPP from The Fourth Hospital of Baotou city, Inner Mongolia Autonomous Region, were collected and divided into general treatment (AF) and probiotic (AFY) groups, according to the treatment of “combined Bifidobacterium, Lactobacillus, Enterococcus, and Bacillus cereus tablets live”. High-throughput 16S rDNA sequencing was used to identify intestinal flora.   Results  Intestinal flora abundance and diversity in children with MPP were decreased. Both Shannon and Simpson indices were lower in the AF group when compared with healthy controls (P < 0.05). When compared with healthy controls, the proportion of Enterorhabdus was lower in the AF group, while the proportion of Lachnoclostridium was higher (P < 0.05). The proportion of Bifidobacteria and Akkermansia was lower in the AFY group but Enterococcus, Lachnoclostridium, Roseburia, and Erysipelatoclostridium proportions were higher. The proportion of Escherichia coli–Shigella in the AFY group after treatment was decreased (P < 0.05).   Conclusions  The intestinal flora of children with MPP is disturbed, manifested as decreased abundance and diversity, and decreased Bifidobacteria. Our probiotic mixture partly improved intestinal flora disorders.
Letter to the Editor
Effects of Hyperbaric Exposure on Hematology and Cecal Microbiota in Rats
ZHANG Chun Hong, DONG Shu Yue, LI Dan, LIU Jun, MAO Wei Chang, FANG Yi Qun, ZHANG Tie Hua
2022, 35(6): 538-542.   doi: 10.3967/bes2022.071
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Evaluation of Insulin Resistance Markers and Their Relationship with ADIPO Q Gene Polymorphism in Clinically Euthyroid Type 2 Diabetic Patients
Rajendran Jayanthi, Anandaraj Lokeshmaran, Ramasamy Sathiya, Abu Raghavan Srinivasan
2022, 35(6): 543-546.   doi: 10.3967/bes2022.072
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Triclosan-induced Oxidative Stress Injury and Apoptosis by Regulating the PI3K/Akt/Caspase-3 Signaling Pathway in Human Renal Glomerular Endothelial Cells
MA Yan, CHEN Chen, WANG Jing Bo, CHENG Jia Li, SHEN Shi, CHEN Xi, HUO Jun Sheng
2022, 35(6): 547-551.   doi: 10.3967/bes2022.073
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Long-Term, Low-Level Microwave Radiation Impairs Learning and Memory via Synbindin: Molecular Basis and Underlying Mechanism
YANG Zhen Zhong, WU Da Wei, DING Bai, MA Hong Bo, FEI Jin Xue, CHENG Liang, ZHAO Ya Li
2022, 35(6): 552-557.   doi: 10.3967/bes2022.074
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Association of Early-Life Famine Exposure with Metabolic Dysfunction-Associated Fatty Liver Disease and Fibrosis in Adulthood
WEI Ran, QI Hong Yan, LIN Lin, ZHU Yuan Yue, ZHANG Yi, ZHANG Jie, WU Xue Yan, HU Chun Yan, WANG Shuang Yuan, LIN Hong, XU Yu, XU Min, BI Yu Fang, WANG Wei Qing, LU Jie Li, NING Guang, CHEN Yu Hong
2022, 35(6): 558-562.   doi: 10.3967/bes2022.075
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Now- and Fore-casting the Secular Epidemiological Trends and Seasonality of the Comeback of Scarlet Fever in China: A 16-year Time Series Analysis
WANG Yong Bin, LI Yan Yan, LU Hao, TAO Ying Jun, LI Yu Hong, WANG Lei, LIANG Wen Juan
2022, 35(6): 563-567.   doi: 10.3967/bes2022.076
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Impact of COVID-19 Pandemic on the Mental Health of Returning Employees in Labor-Intensive Companies: A Study Based on Repeated Surveys
LIU Yu, WANG Xiao Feng, XI Jun Yan, XIAO Qi Peng, HE Li Ping, LU Ci Yong, HE Ling
2022, 35(6): 568-572.   doi: 10.3967/bes2022.077
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Bone Injury and Fracture Healing Biology
Ahmad Oryan, Somayeh Monazzah, Amin Bigham-Sadegh
2015, 28(1): 57-71.   doi: 10.3967/bes2015.006
[Abstract](5733) [PDF 3875KB](2231)
The Serum Exosome Derived MicroRNA-135a, -193b, and-384 Were Potential Alzheimer's Disease Biomarkers
YANG Ting Ting, LIU Chen Geng, GAO Shi Chao, ZHANG Yi, WANG Pei Chang
2018, 31(2): 87-96.   doi: 10.3967/bes2018.011
[Abstract](5526) [FullText HTML](1612) [PDF 11333KB](1612)
Stability of SARS Coronavirus in Human Specimens and Environment and Its Sensitivity to Heating and UV Irradiation
2003, 16(3): 246-255.  
[Abstract](5843) [PDF 610KB](1201)
Burden of Cirrhosis and Other Chronic Liver Diseases Caused by Specific Etiologies in China, 1990−2016: Findings from the Global Burden of Disease Study 2016
LI Man, WANG Zhuo Qun, ZHANG Lu, ZHENG Hao, LIU Dian Wu, ZHOU Mai Geng
2020, 33(1): 1-10.   doi: 10.3967/bes2020.001
[Abstract](4418) [FullText HTML](1185) [PDF 2552KB](1185)
Effects of Short-Term Forest Bathing on Human Health in a Broad-Leaved Evergreen Forest in Zhejiang Province, China
MAO Gen Xiang, LAN Xiao Guang, CAO Yong Bao, CHEN Zhuo Mei, HE Zhi Hua, LV Yuan Dong, WANG Ya Zhen, HU Xi Lian, WANG Guo Fu, YAN Jing
2012, 25(3): 317-324.   doi: 10.3967/0895-3988.2012.03.010
[Abstract](3052) [PDF 528KB](1124)
Metabolomic Profiling Differences among Asthma, COPD, and Healthy Subjects: A LC-MS-based Metabolomic Analysis
LIANG Ying, GAI Xiao Yan, CHANG Chun, ZHANG Xu, WANG Juan, LI Ting Ting
2019, 32(9): 659-672.   doi: 10.3967/bes2019.085
[Abstract](3390) [FullText HTML](753) [PDF 2914KB](753)
Evaluating the Nutritional Status of Oncology Patientsand Its Association with Quality of Life
ZHANG Ya Hui, XIE Fang Yi, CHEN Ya Wen, WANG Hai Xia, TIAN Wen Xia, SUN Wen Guang, WU Jing
2018, 31(9): 637-644.   doi: 10.3967/bes2018.088
[Abstract](4003) [FullText HTML](883) [PDF 31943KB](883)
Protein Requirements in Healthy Adults:A Meta-analysis of Nitrogen Balance Studies
LI Min, SUN Feng, PIAO Jian Hua, YANG Xiao Guang
2014, 27(8): 606-613.   doi: 10.3967/bes2014.093
[Abstract](2464) [PDF 8784KB](898)
Resveratrol Induces Apoptosis and Autophagy in T-cell Acute Lymphoblastic Leukemia Cells by Inhibiting Akt/mTOR and Activating p38-MAPK
GE Jiao, LIU Yan, LI Qiang, GUO Xia, GU Ling, MA Zhi Gui, ZHU Yi Ping
2013, 26(11): 902-911.   doi: 10.3967/bes2013.019
[Abstract](3267) [PDF 12143KB](607)
The Emergence, Epidemiology, and Etiology of Haff Disease
PEI Pei, LI Xiao Yan, LU Shuang Shuang, LIU Zhe, WANG Rui, LU Xuan Cheng, LU Kai
2019, 32(10): 769-778.   doi: 10.3967/bes2019.096
[Abstract](3186) [FullText HTML](1220) [PDF 2249KB](1220)
Supplementation of Fermented Barley Extracts with Lactobacillus Plantarum dy-1 Inhibits Obesity via a UCP1-dependent Mechanism
XIAO Xiang, BAI Juan, LI Ming Song, ZHANG Jia Yan, SUN Xin Juan, DONG Ying
2019, 32(8): 578-591.   doi: 10.3967/bes2019.076
[Abstract](3453) [FullText HTML](586) [PDF 9101KB](586)
Inactivation of Poliovirus by Ozone and the Impact of Ozone on the Viral Genome
JIANG Han Ji, CHEN Na, SHEN Zhi Qiang, YIN Jing, QIU Zhi Gang, MIAO Jing, YANG Zhong Wei, SHI Dan Yang, WANG Hua Ran, WANG Xin Wei, LI Jun Wen, YANG Dong, JIN Min
2019, 32(5): 324-333.   doi: 10.3967/bes2019.044
[Abstract](3266) [FullText HTML](714) [PDF 2666KB](714)
Mutual Impact of Diabetes Mellitus and Tuberculosis in China
CHENG Jun, ZHANG Hui, ZHAO Yan Lin, WANG Li Xia, CHEN Ming Ting
2017, 30(5): 384-389.   doi: 10.3967/bes2017.051
[Abstract](2720) [FullText HTML](823) [PDF 671KB](823)
Health Effect of Forest Bathing Trip on Elderly Patients with Chronic Obstructive Pulmonary Disease
JIA Bing Bing, YANG Zhou Xin, MAO Gen Xiang, LYU Yuan Dong, WEN Xiao Lin, XU Wei Hong, LYU XIAO Ling
2016, 29(3): 212-218.   doi: 10.3967/bes2016.026
[Abstract](1531) [PDF 803KB](517)
Exposure Effects of Terahertz Waves on Primary Neurons and Neuron-like Cells Under Nonthermal Conditions
TAN Sheng Zhi, TAN Peng Cheng, LUO Lan Qing, CHI Yun Liang, YANG Zi Long, ZHAO Xue Long, ZHAO Li, DONG Ji, ZHANG Jing, YAO Bin Wei, XU Xin Ping, TIAN Guang, CHEN Jian Kui, WANG Hui, PENG Rui Yun
2019, 32(10): 739-754.   doi: 10.3967/bes2019.094
[Abstract](2936) [FullText HTML](780) [PDF 3322KB](780)
Hypertension Prevalence, Awareness, Treatment, and Control and Their Associated Socioeconomic Factors in China: A Spatial Analysis of A National Representative Survey
WANG Wei, ZHANG Mei, XU Cheng Dong, YE Peng Peng, LIU Yun Ning, HUANG Zheng Jing, HU Cai Hong, ZHANG Xiao, ZHAO Zhen Ping, LI Chun, CHEN Xiao Rong, WANG Li Min, ZHOU Mai Geng
2021, 34(12): 937-951.   doi: 10.3967/bes2021.130
[Abstract](550) [FullText HTML](236) [PDF 2205KB](236)
TaqMan Real-time RT-PCR Assay for Detecting and Differentiating Japanese Encephalitis Virus
SHAO Nan, LI Fan, NIE Kai, FU Shi Hong, ZHANG Wei Jia, HE Ying, LEI Wen Wen, WANG Qian Ying, LIANG Guo Dong, CAO Yu Xi, WANG Huan Yu
2018, 31(3): 208-214.   doi: 10.3967/bes2018.026
[Abstract](2056) [FullText HTML](668) [PDF 4691KB](668)
Trends in Lipids Level and Dyslipidemia among Chinese Adults, 2002-2015
SONG Peng Kun, MAN Qing Qing, LI Hong, PANG Shao Jie, JIA Shan Shan, LI Yu Qian, HE Li, ZHAO Wen Hua, ZHANG Jian
2019, 32(8): 559-570.   doi: 10.3967/bes2019.074
[Abstract](3041) [FullText HTML](701) [PDF 3641KB](701)
Paraben Content in Adjacent Normal-malignant Breast Tissues from Women with Breast Cancer
Mohammad Mehdi Amin, Maryam Tabatabaeian, Afsane Chavoshani, Elham Amjadi, Majid Hashemi, Karim Ebrahimpour, Roya Klishadi, Sedigheh Khazaei, Marjan Mansourian
2019, 32(12): 893-904.   doi: 10.3967/bes2019.112
[Abstract](3100) [FullText HTML](942) [PDF 2461KB](942)
Outline of the Report on Cardiovascular Disease in China, 2010
HU Sheng Shou, KONG Ling Zhi, GAO Run Lin, ZHU Man Lu, WANG Wen, WANG Yong Jun, WU Zhao Su, CHEN Wei Wei, LIU Ming Bo
2012, 25(3): 251-256.   doi: 10.3967/0895-3988.2012.03.001
[Abstract](3876) [PDF 2961KB](676)

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Current Issue

Vol 35, No 6

(June, 2022)

ISSN 0895-3988

CN 11-2816/Q

  • 2021 Impact Factor 2.831

  • 2021 Journal Citation Reports