Articles in press have been peer-reviewed and accepted, which are not yet assigned to volumes /issues, but are citable by Digital Object Identifier (DOI).
The Regulatory Role and Mechanism of Circadian Rhythm in Hemoglobin Co-cultured Neurovascular Unit
Fang Xue, Wenchao Chen, Xia Lian, Guanghui He, Jingyuan Tian, Yinghong Liu, Gaiqing Wang
In press  doi: 10.3967/bes2024.090
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  Objective  Intracranial hemorrhage (ICH), the second most common subtype of stroke, exacerbates the disruption of the blood-brain barrier (BBB), leading to vasogenic edema, plasma protein extravasation, and infiltration of neurotoxic substances. The clearance capacity of the brain plays a crucial role in maintaining BBB homeostasis and facilitating patient recovery after hemorrhage. This study aimed to investigate the effect of circadian rhythms on BBB function, neuronal damage, and clearance capabilities.  Methods  The transwell model and hemoglobin were co-cultured to simulate the BBB environment after ICH. After intervention with different light groups, neuronal apoptosis was determined, glial phagocytosis was analyzed, the expression of endogenous clearing-related proteins aquaporin 4 (AQP4) and low-density lipoprotein receptor-related protein 1 (LRP1) was detected by western blotting and immunofluorescence dual standard method, and the expression of the tight junction protein occludin and melatonin receptor 1A (MTNR1A) was quantitatively analyzed.  Results  Circadian rhythms play a key role in maintaining the integrity of the BBB, reducing oxidative stress-induced neuronal damage, and improving microglial phagocytosis. Meanwhile, the expression of occludin and MTNR1A in neurovascular unit (NVU) co-cultured with hemoglobin improved the expression of AQP4 and LRP1, the key proteins in the NVU’s endogenous brain clearance system.  Conclusion  Circadian rhythm (alternating black and white light) protects the NVU BBB function after ICH, promotes the expression of proteins related to the clearance of the hematoma, provides new evidence for the clinical treatment of patients recovering from ICH, and improves the circadian rhythm to promote brain metabolism and hematoma clearance.
Prospective Cohort Investigation on Physical Activity of Osteoporosis Outcomes (PAOPO) in Jidong: Objectives, Study Design, and Baseline Characteristics
Yang Jingzhi, Shen Hao, Wang Sicheng, Bai Long, Geng Zhen, Jing Yingying, Xu Ke, Zhang Hao, Zhang Yuanwei, Li Zuhao, Wang Chuandong, Wang Guangchao, Chen Xiao, Su Jiacan
In press  doi: 10.3967/bes2024.117
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  Objective  The aim of this study was to investigate the prospective association between physical activity (PA), independently or in conjunction with other contributing factors, and osteoporosis (OP) outcomes.  Methods  The Physical Activity in Osteoporosis Outcomes study was a community-based cohort investigation. A structured questionnaire was used to gather the participants’ sociodemographic characteristics. Bone mineral density (BMD) measurements were performed to assess OP outcomes, and the relationship between BMD and OP was evaluated within this cohort.  Results  From 2013 to 2014, 8,471 participants aged 18 years and older were recruited from Tangshan, China’s Jidong community. Based on their PA level, participants were categorized as inactive, moderately active, or very active. Men showed higher physical exercise levels than women across the activity groups. BMD was significantly higher in the very active group than in the moderately active and inactive groups. Individuals aged > 50 years are at a higher risk of developing OP and osteopenia.  Conclusion  The Physical Activity in Osteoporosis Outcomes study offers promising insights into the relationship between PA and OP outcomes, encouraging the implementation of PA in preventing and managing OP.
Spatial Heterogeneity and Risk Factors of Dental Caries in 12-Year-Old Children in Shanxi Province, China
HOU Ru Xia, YANG Ting Ting, LIU Jia Jia, CHEN Hao, KANG Wen, LI Jun Ming, SHI Xiao Tong, LIANG Yi, LIU Jun Yu, ZHAO Bin, WANG Xiang Yu
In press  doi: 10.3967/bes2024.102
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  Objective   This study aimed to explore the spatial heterogeneity and risk factors for dental caries in 12-year-old children in Shanxi province , China.  Methods   The data encompassed 3,721 participants from the two most recent oral health surveys conducted across 16 districts in Shanxi Province in 2015 and 2018. Eighteen specific variables were analyzed to examine the interplay between socioeconomic factors, medical resources and environmental conditions. The Geo-detector model was employed to assess the impacts and interactions of these ecological factors.  Results   Socioeconomic factors (Q = 0.30, P < 0.05) exhibited a more substantial impact compared to environmental (Q = 0.19, P < 0.05) and medical resource factors (Q = 0.25, P < 0.05). Notably, the urban population percentage (UPP) demonstrated the most significant explanatory power for the spatial heterogeneity in caries prevalence, as denoted by its highest q-value (q = 0.51, P < 0.05). Additionally, the spatial distribution’s heterogeneity of caries was significantly affected by SO2 concentration (q = 0.39, P < 0.05) and water fluoride levels (q = 0.27, P < 0.05) among environmental factors.  Conclusion   The prevalence of caries exhibited spatial heterogeneity, escalating from North to South in Shanxi Province, China, influenced by socioeconomic factors, medical resources, and environmental conditions to varying extents.
DNA Methylation of KLRC1 and KLRC3 in Autoimmune Thyroiditis: Perspective of Different Water Iodine Exposure
CHEN Yao, LIU Jin Jin, QU Meng Ying, REN Bing Xuan, WU Huai Yong, ZHANG Li, ZHOU Zheng, LIU Li Xiang, SHEN Hong Mei
In press  doi: 10.3967/bes2024.103
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  Objective   This study aimed to identify differentially methylated genes (DMGs) associated with natural killer cells in patients with autoimmune thyroiditis (AIT), focusing on the influence of varying water iodine exposure levels.  Methods   Participants were divided into categories based on median water iodine (MWI) concentrations: iodine-fortified areas (IFA, MWI < 10 µg/L), iodine-adequate areas (IAA, 40 ≤ MWI ≤ 100 µg/L), and iodine-excessive areas (IEA, MWI > 300 µg/L). A total of 176 matched AIT cases and controls were recruited and divided into 89, 40, and 47 pairs for IFA, IAA, and IEA, respectively. DMGs were identified using 850k BeadChip analysis for 10/10 paired samples. Validation of DNA methylation and mRNA expression levels of the DMGs was conducted using MethylTarget™ and QRT-PCR for 176/176 paired samples.  Results   KLRC1, KLRC3, and SH2D1B were identified as significant DMGs. Validation revealed that KLRC1 was hypomethylated and highly expressed, whereas KLRC3 was hypermethylated and highly expressed in individuals with AIT. Furthermore, KLRC1 was hypomethylated and highly expressed in both IFA and IEA.  Conclusion   The DNA methylation status of KLRC1 and KLRC3 may play crucial roles in AIT pathogenesis. Additionally, DNA methylation of KLRC1 seems to be influenced by different iodine concentrations in water.
blaNDM-1 Carried by a Transferable Plasmid in a Salmonella Strain Isolated from Healthy Individuals
ZENG Wei, LUO Ming, DU Pengcheng, LI Zhenpeng, PENG Yao, WANG Mengyu, ZHAO Wenxuan, ZHANG Huayao, LI Yang, LUO Pengjie, WU Yannong, XU Jialiang, LI Xu, LU Xin, KAN Biao
In press  doi: 10.3967/bes2024.104
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  Objectives   Our study aimed to conduct genomic characterization of Salmonella strains carrying the blaNDM-1 gene in the intestinal tract of healthy individuals. The objectives were to underscore the importance of genomic surveillance for drug resistance in both commensal and pathogenic bacteria among healthy populations, and to establish protocols for regulating drug resistance plasmids based on the completion of a comprehensive map of drug resistance plasmid genomes.  Methods  We performed antimicrobial susceptibility testing and employed second- and third-generation sequencing techniques to analyze Salmonella strains harboring the blaNDM-1 gene, to surveil drug-resistant bacteria in the intestines of healthy subjects. Sequence comparison was conducted using both core- and pan-genome approaches. Concurrently, conjugation experiments were carried out to assess the efficiency of plasmid transfer.  Results  We isolated a carbapenem-resistant Salmonella enterica serovar Typhimurium strain from a healthy food worker in China. This strain harbored an IncHI2/IncHI2A plasmid carrying blaNDM-1 along with multiple antibiotic resistance genes (ARGs). Our findings highlight the potential for asymptomatic carriers to facilitate the transmission of ARGs. Pan-genomic analysis revealed that blaNDM-1-positive plasmids could traverse bacterial species barriers, facilitating cross-host transmission.  Conclusions  This study marks the first detection of blaNDM-1 in Salmonella strains isolated from healthy individuals. We underscore the risk associated with the transmission of conjugative hybrid plasmids carrying blaNDM-1, which have the potential to be harbored and transmitted among healthy individuals. Enhanced surveillance of drug-resistant pathogens and plasmids in the intestinal microbiota of healthy individuals could provide insights into the risk of ARG transmission and pathways for population-wide dissemination via ARG transfer factors.
Predicting Acute Mountain Sickness Using Regional Sea-Level Cerebral Blood Flow
ZHANG Hao, FENG Jie, ZHANG Shi Yu, LIU Wen Jia, MA Lin
In press  doi: 10.3967/bes2024.100
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  Objective   To investigate the role of sea-level cerebral blood flow (CBF) in predicting acute mountain sickness (AMS) using three-dimensional pseudo-continuous arterial spin labeling (3D-pCASL).  Methods  Forty-eight healthy volunteers reached an altitude of 3650 m by air after undergoing a head magnetic resonance imaging (MRI) including 3D-pCASL at sea level. The CBF values of the bilateral anterior cerebral artery (ACA), middle cerebral artery (MCA), posterior cerebral artery (PCA), and posterior inferior cerebellar artery (PICA) territories and the laterality index (LI) of CBF were compared between the AMS and non-AMS groups. Statistical analyses were performed to determine the relationship between CBF and AMS, and the predictive performance was assessed using receiver operating characteristic (ROC) curves.  Results   The mean cortical CBF in women (81.65±2.69 ml/100 g/min) was higher than that in men (74.35±2.12 ml/100 g/min) (P < 0.05). In men, the cortical CBF values in the bilateral ACA, PCA, PICA, and right MCA were higher in patients with AMS than in those without. Cortical CBF in the right PCA best predicted AMS (AUC = 0.818). In women, the LI of CBF in the ACA was different between the AMS and non-AMS groups and predicted AMS with an AUC of 0.753.  Conclusion   Although the mechanism and prediction of AMS are quite complicated, higher cortical CBF at sea level, especially the CBF of the posterior circulatory system, may be used for prediction in male volunteers using non-invasive 3D-pCASL.
Changes on Stroke Burden Attributable to Ambient Fine Particulate Matter in China
WANG Jing Yu, WANG Yan, LIANG Xiao Hua, HUANG Ke Yong, LIU Fang Chao, CHEN Shu Feng, LU Xiang Feng, LI Jian Xin
In press  doi: 10.3967/bes2024.083
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  Objective  In recent decades, China has implemented a series of policies to address air pollution. We aimed to assess the health effects of these policies on stroke burden attributable to ambient fine particulate matter (PM2.5).  Methods  Joinpoint regression was applied to explore the temporal tendency of stroke burden based on data from the Global Burden of Disease 2019 study.  Results  The age-standardized rates of disability-adjusted life year (DALY) for stroke attributable to ambient PM2.5 in China, increased dramatically during 1990−2012, subsequently decreased at an annual percentage change (APC) of −1.98 (95% confidence interval [CI]: −2.26, −1.71) during 2012−2019. For ischemic stroke (IS), the age-standardized DALY rates doubled from 1990 to 2014, and decreased at an APC of −0.83 (95% CI: −1.33, −0.33) during 2014−2019. Intracerebral hemorrhage (ICH) showed a substantial increase in age-standardized DALY rates from 1990 to 2003, followed by declining trends, with APCs of −1.46 (95% CI: −2.74, −0.16) during 2003−2007 and −3.33 (95% CI: −3.61, −3.06) during 2011−2019, respectively. Conversely, the age-standardized DALY rates for subarachnoid hemorrhage (SAH) generally declined during 1990−2019.   Conclusion  Our results clarified the dynamic changes of the ambient PM2.5−attributable stroke burden in China during 1990−2019, highlighting the health effects of air quality improvement policies.
Effects of curcumin on neuroinflammation and the Nrf2/HO-1 pathway in rat brains following gas explosion
Dong Xinwen, Su Yaguang, Luo Zheng, Deng Lyufei, Xiao Feng HAN, Liang Yifang, YAO Sanqiao, Wu Weidong, Cao Jia, Tian Linqiang, Bai Yichun, Ren Wenjie
In press  doi: 10.3967/bes2024.116
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Gas explosions, a major occupational hazard in China’s coal industry, endanger the lives and health of miners. These explosions cause a specific type of traumatic brain injury with complex mechanisms, leading to disability and death. A study by Zhao et al. using magnetic resonance imaging on 49 gas explosion survivors revealed significant damage to brain regions like the hippocampus and cerebral cortex. Similarly, our recent animal experiments conducted in a real tunnel environment demonstrated that gas explosions alter rat neurobehavior, cause microscopic structural damage to brain tissue, and impaired function[1]. However, the underlying physiological and pathological mechanisms of this blast-induced brain injury remain unclear.
Estimation of Incidence of Herpes Zoster in Three Cities of China, 2019-2020
Zhang Qian, Qin Wei, Huang Zhenshui, Liang Lilin, Hu Qianqian, Wang Yao, Pan Fan, Cui Feng, Liu Xinli, Tang Lin, Wang Xiaoqi, Ma Chao, Wang Fuzhen, Yin Zundong
In press  doi: 10.3967/bes2024.115
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Multivariate Analysis of Female Stress Urinary Incontinence and Establishment of a Prediction Model
Lei Li, Lin Luo, Junnai Wang, Ying Hong, Jianfang Geng
In press  doi: 10.3967/bes2024.114
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The Effect of Blood Lipid Profiles on Chronic Kidney Disease in a Prospective Cohort: Based on a Regression Discontinuity Design
Kang Lyu, Shaodong Liu, Yanli Liu, Jinlong You, Xue Wang, Min Jiang, Chun Yin, Desheng Zhang, Yana Bai, Minzhen Wang, Shan Zheng
In press  doi: 10.3967/bes2024.113
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  Objective   Previous studies on the association between lipid profiles and chronic kidney disease (CKD) have yielded inconsistent results and no defined thresholds for blood lipids.  Methods   A prospective cohort study including 32,351 subjects who completed baseline and follow-up surveys over 5 years was conducted. Restricted cubic splines and Cox models were used to examine the association between the lipid profiles and CKD. A regression discontinuity design was used to determine the cutoff value of lipid profiles that was significantly associated with increased the risk of CKD.  Results   Over a median follow-up time of 2.2 (0.5, 4.2) years, 648 (2.00%) subjects developed CKD. The lipid profiles that were significantly and linearly related to CKD included total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), TC/HDL-C, and TG/HDL-C, whereas low-density lipoprotein cholesterol (LDL-C) and LDL-C/HDL-C were nonlinearly correlated with CKD. TC, TG, TC/HDL-C, and TG/HDL-C showed an upward jump at the cutoff value, increasing the risk of CKD by 0.90%, 1.50%, 2.30%, and 1.60%, respectively, whereas HDL-C showed a downward jump at the cutoff value, reducing this risk by 1.0%. Female and participants with dyslipidemia had a higher risk of CKD, while the cutoff values for the different characteristics of the population were different.  Conclusions   There was a significant association between lipid profiles and CKD in a prospective cohort from Northwest China, and TG, TC/HDL-C, while TG/HDL-C showed a stronger risk association. The specific cutoff values of lipid profiles may provide a clinical reference for screening or diagnosing CKD risk.
Nogo-A Protein Mediates Oxidative Stress and Synaptic Damage Induced by High-altitude Hypoxia in the Rat Hippocampus
Jinyu Fang, Huaicun Liu, Yanfei Zhang, Quancheng Cheng, Ziyuan Wang, Xuan Fang, Huiru Ding, Weiguang Zhang, Chunhua Chen
In press  doi: 10.3967/bes2024.112
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  Objective  High-altitude hypoxia exposure often damages hippocampus-dependent learning and memory. Nogo-A is an important axonal growth inhibitory factor. However, its function in high-altitude hypoxia and its mechanism of action remain unclear.  Methods  In an in vivo study, a low-pressure oxygen chamber was used to simulate high-altitude hypoxia, and genetic or pharmacological intervention was used to block the Nogo-A/NgR1 signaling pathway. Contextual fear conditioning and Morris water maze behavioral tests were used to assess learning and memory in rats, and synaptic damage in the hippocampus and changes in oxidative stress levels were observed. In vitro, SH-SY5Y cells were used to assess oxidative stress and mitochondrial function with or without Nogo-A knockdown in Oxygen Glucose-Deprivation/Reperfusion (OGD/R) models.  Results  Exposure to acute high-altitude hypoxia for 3 or 7 days impaired learning and memory in rats, triggered oxidative stress in the hippocampal tissue, and reduced the dendritic spine density of hippocampal neurons. Blocking the Nogo-A/NgR1 pathway ameliorated oxidative stress, synaptic damage, and the learning and memory impairment induced by high-altitude exposure.  Conclusion  Our results demonstrate the detrimental role of Nogo-A protein in mediating learning and memory impairment under high-altitude hypoxia and suggest the potential of the Nogo-A/NgR1 signaling pathway as a crucial therapeutic target for alleviating learning and memory dysfunction induced by high-altitude exposure.
Mito-TEMPO Ameliorates Sodium Palmitate Induced Ferroptosis in MIN6 Cells through PINK1/Parkin-Mediated Mitophagy
Baolei Chang, Yanyu Su, Tingting Li, Yanxia Zheng, Ruirui Yang, Heng Lu, Hao Wang, Yusong Ding
In press  doi: 10.3967/bes2024.111
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  Objective   Mitochondrial reactive oxygen species (mtROS) could cause damage to pancreatic β-cells, rendering them susceptible to oxidative damage. Hence, investigating the potential of the mitochondria-targeted antioxidant (Mito-TEMPO) to protect pancreatic β-cells from ferroptosis by mitigating lipid peroxidation becomes crucial.  Methods   MIN6 cells were cultured in vitro with 100 μmol/L sodium palmitate (SP) to simulate diabetes. FerroOrange was utilized for the detection of Fe2+ fluorescence staining, BODIPY581/591C11 for lipid reactive oxygen species, and MitoSox-Red for mtROS. Alterations in mitophagy levels were assessed through the co-localization of lysosomal and mitochondrial fluorescence. Western blotting was employed to quantify protein levels of Acsl4, GPX4, FSP1, FE, PINK1, Parkin, TOMM20, P62, and LC3. Subsequently, interventions were implemented using Mito-TEMPO and Carbonyl cyanide 3-chlorophenylhydrazone (CCCP) to observe changes in ferroptosis and mitophagy within MIN6 cells.  Results   We found that SP induced a dose-dependent increase in Fe2+ and lipid ROS in MIN6 cells while decreasing the expression levels of GPX4 and FSP1 proteins. Through bioinformatics analysis, it has been uncovered that mitophagy assumes a crucial role within the ferroptosis pathway associated with diabetes. Additionally, SP decreased the expression of mitophagy-related proteins PINK1 and Parkin, leading to mtROS overproduction. Conversely, Mito-TEMPO effectively eliminated mtROS while activating the mitophagy pathways involving PINK1 and Parkin, thereby reducing the occurrence of ferroptosis in MIN6 cells. CCCP also demonstrated efficacy in reducing ferroptosis in MIN6 cells.  Conclusion   In summary, Mito-TEMPO proved effective in attenuating mtROS production and initiating mitophagy pathways mediated by PINK1 and Parkin in MIN6 cells. Consequently, this decreased iron overload and lipid peroxidation, ultimately safeguarding the cells from ferroptosis.
Summary Analysis of National Surveillance on Kashin–Beck disease from 1990 to 2023
Silu Cui, Hui Liu, Junrui Pei, Jiaxin Li, Zhe Jiao, Qing Deng, Ning Liu, Yanhong Cao, Jun Yu
In press  doi: 10.3967/bes2024.109
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  Objectives  To analyze the epidemiological characteristics and epidemic situation of children with Kashin–Beck disease (KBD) in China, and provide the basis for formulating prevention and control measures.  Methods  Fixed-point monitoring, moving-point monitoring, and full coverage of monitoring were promoted successively from 1990 to 2023. Some children (7–12 years old) underwent clinical and right-hand X-ray examinations every year. According to the KBD diagnosis criteria, clinical and X-ray assessments were used to confirm the diagnosis.  Results  In 1990, the national KBD detectable rate was 21.01%. X-ray detection decreased to below 10% in 2003 and below 5% in 2007. Between 2010 and 2018, the prevalence of KBD in children was less than 0.4%, which fluctuated at a low level, and has decreased to 0% since 2019. Spatial epidemiological analysis indicated a spatial clustering of adult patients prevalence rate in the KBD areas.  Conclusion  The evaluation results of the elimination of KBD in China over the last 2 years showed that all villages in the monitored areas have reached the elimination standard. While the adult KBD patients still need for policy consideration and care.
A miR-100 Polymorphism Signature is Protectively Associated with Hematological Abnormalities in Individuals Exposed to Benzene, Toluene, Ethylbenzene, and Xylene
Farnaz Nourmohammadian Dehkordi, Samaneh Jafari Roshan, Amin Yousefvand, Behnam Mansoori, Yaser Mansoori, Abdolreza Daraei
In press  doi: 10.3967/bes2024.110
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  Objective  The DNA double-strand break (DSB) repair system plays a key role in eliminating DNA damage in hematopoietic cells caused by benzene, toluene, ethylbenzene, and xylene (BTEX) compounds, and the polymorphisms of genes controlling this DNA repair system are linked to the different genetic susceptibilities of individuals to respond to the effects of BTEXs. In addition, there is some evidence that BTEX can induce hematological abnormalities through changing the function of micro RNAs regulating the DSB system, but there is no report on the association of their polymorphisms with BTEX-related disorders. This study aimed to explore the relationship between important polymorphisms in miRNA-100 and miRNA-506, two key regulators of the hematopoietic DSB system, including miRNA-100 RS543412 C>T and miRNA-506 RS5905008 A>G, and hematological abnormalities in BTEX-exposed workers.  Methods  This study included 138 patients who were exposed to BTEX compounds and had one or more abnormalities in their hematological indices and 145 healthy individuals with the same exposure conditions but without any abnormal hematological defects. Polymorphism genotyping was performed using polymerase chain reaction restriction fragment length polymorphism.  Results  The results revealed that frequency of the T allele as well as the TT and CT genotypes of the miR-100-RS543412C>T single nucleotide polymorphism was significantly lower in patients than in healthy participants (allelic P = 0.005; genotype P = 0.013). Moreover, individuals with TT and CT genotypes in the codominant (CT vs. CC, OR: 0.546, P = 0.034; TT vs. CC, OR: 0.335, P = 0.004), dominant (CT+TT vs. CC, OR: 0.486, P = 0.008), and recessive (TT vs. CC+CT, OR: 0.507, P = 0.038) models had a lower risk of hematological abnormalities. We also observed that the combined heterozygous genotype of the two polymorphisms was significantly different (P = 0.028) between the two groups and was associated with a reduced risk of abnormalities in blood indices (OR: 0.089, P = 0.03).  Conclusion  These findings suggest that this miR-single nucleotide polymorphism may be a protective non-coding signature underlying the risk of BTEX exposure-related hematological abnormalities. However, this hypothesis requires further investigation.
Willingness to Pay for Antiretroviral Drugs Covered by Medical Insurance among People Living with HIV in 18 Chinese Cities
HU Jing Kun, TANG Hou Lin, KANG Wen Ting, WANG Shu Yu, XU Jie, ZHAO De Cai, HAO Yang, WANG Xin Lun, LYU Fan, ZHANG Guang, XU Peng
In press  doi: 10.3967/bes2024.105
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  Objective   Antiretroviral drugs covered by medical insurance have been gradually used by people living with human immunodeficiency virus (PLWH) in recent years in China. This study aimed to analyze their willingness to pay (WTP) for antiretroviral drugs.  Methods   A mixed-methods study design involving a cross-sectional survey and in-depth interviews was conducted. A cross-sectional survey was performed to collect data on the general characteristics, economic status, antiretroviral therapy (ART) status, and WTP of PLWH in 18 Chinese cities from August 2022 to February 2023. Multivariate logistic regression was used to analyze the factors associated with WTP. Representatives of PLWH were interviewed via in-depth interviews, and the data were thematically analyzed.  Results   Among the 941 PLWH, 271 (28.80%) were willing to pay for antiretroviral drugs covered by medical insurance. For basic medical insurance for urban and rural residents, PLWH with the following characteristics were more willing to pay: an educational level of senior high school or technical secondary school, having an undergraduate degree or higher, frequently working away from their hometowns, and homosexual transmission. Off-farm workers and recipients of government medical aid were more unwilling to pay. For basic medical insurance for urban employees, PLWH with the following characteristics were more willing to pay: frequently working away from their hometowns; homosexual transmission; personal annual income ≥100,000 CNY; and adverse events of antiretroviral drugs. The main reasons for PLWH’s WTP for antiretroviral drugs covered by medical insurance were that the drugs had fewer adverse events and were easier to administer. The main reasons for PLWH’s unwillingness to pay were financial difficulties and privacy concerns.  Conclusion   Nearly one-third of PLWH are willing to pay for antiretroviral drugs covered by medical insurance. In the future, PLWH with a high WTP can be guided to use these drugs.
Spatial Dynamics of Chickenpox Outbreaks in Rapidly Developing Regions: Implications for Global Public Health
WANG Li, WANG Miao Miao, XU Cheng Dong, WANG Pei Han, YOU Mei Ying, LI Zi Han, CHEN Xin Mei, LIU Xin Yu, LI Xu Dong, WANG Yuan Yuan, HU Yue Hua, YIN Da Peng
In press  doi: 10.3967/bes2024.068
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  Objective  The occurrence of chickenpox in rapidly developing areas poses substantial seasonal risk to children. However, certain factors influencing local chickenpox outbreaks have not been studied. Here, we examined the relationship between spatial clustering, heterogeneity of chickenpox outbreaks, and socioeconomic factors in Southern China.  Methods  We assessed chickenpox outbreak data from Southern China between 2006 and 2021, comprising both relatively fast-growing parts and slower sub-regions, and provides a representative sample of many developing regions. We analyzed the spatial clustering attributes associated with chickenpox outbreaks using Moran’s I and local indicators of spatial association and quantified their socioeconomic determinants using Geodetector q statistics.  Results  There were significant spatial heterogeneity in the risk of chickenpox outbreaks, with strong correlations between chickenpox risk and various factors, particularly demographics and living environment. Furthermore, interactive effects among specific are factors, such as population density and per capita residential building area, percentage of households with toilets, percentage of rental housing, exhibited q statistics of 0.28, 0.25, and 0.24, respectively.  Conclusion  This study provides valuable insights into the spatial dynamics of chickenpox outbreaks in rapidly developing regions, revealing the socioeconomic factors affecting disease transmission. These implications extend the formulation of effective public health strategies and interventions to prevent and control chickenpox outbreaks in similar global contexts.
Disparities in the Levels of Whole-blood Epstein-Barr Virus between the Cancer and Non-cancer Populations in Zhejiang, China
JIA Qing Jun, ZENG Mei Chun, CHEN Qi
In press  doi: 10.3967/bes2024.101
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  Objective  This study aimed to investigate the prevalence of Epstein-Barr virus (EBV) infection in patients with and without cancer.  Methods  A total of 26,648 participants who underwent whole-blood EBV DNA (WBEBV) assays between January 1, 2020, and August 31, 2023, were included. The chi-square test was used for categorical data analysis, and R software was used to analyze the differences in EBV DNA load levels and the diagnostic capabilities of WBEBV.  Results  Positive rates were 10.2% and 25.4% for healthy controls (HC) and patients, respectively. The positivity rate for EBV-associated neoplasms (EN) was the highest at 7.53%, followed by leukemia (Le) at 5.49%. The subgroup analysis showed that the positivity rate for abnormal proliferation or hyperplasia (APH) was 31.9%, followed by 30.5% for Le. The WBEBV of patients with transplants (TP), especially living-related transplants (LT), was the highest among all subgroups. WBEBV at diagnosis was used to differentiate between infectious mononucleosis (IM) and chronic active Epstein-Barr virus (CAEBV), with a sensitivity of 67.4% (95% confidence interval [CI]: 57.6–75.8) and specificity of 72% (95% CI: 63.3–79.3). We conclude that the prevalence of EBV infection is low in the healthy population in this region and that a high EBV load at bas`eline is more common in LT, IM, and Lymphocyte Leukemia (LL).  Conclusion  This study used a large-sample survey to characterize the prevalence of whole-blood EBV levels in various diseases, including the stages and subtypes. The EBV detection rate was higher in patients with malignant disease than in those with benign disease. Our study provides clinicians with baseline information regarding EBV-associated diseases.
Letter to the Editor Synaptic Transmission of Primary Hippocampal Neurons was Enhanced after Terahertz Waves Exposure
SONG Le Quan, HE Zhi Wei, PAN Jun Miao, DONG Ji, WANG Hao Yu, ZHANG Jing, YAO Bin Wei, XU Xin Ping, WANG Hui, ZHAO Li, PENG Rui Yun
In press  doi: 10.3967/bes2024.099
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Molecular investigation of Theileria in ixodid ticks from Changzhi, Shanxi Province, China
CUI Jia, RAO Hua Xiang, LIU Yi Ping, LI Dong Mei, YU Juan
In press  doi: 10.3967/bes2024.098
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Cover
2024-6 Cover
2024, 37(6).  
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Contents
2024-6 Contents
2024, 37(6): 1-2.  
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Message from the New Editor-in-Chief
Xiaoming Shi
2024, 37(6): 559-562.   doi: 10.3967/bes2024.108
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Uphold the Original Intention and Work together for Future Development — Speech at the 35th Anniversary Symposium of BES
Dr. Junshi Chen
2024, 37(6): 563-564.   doi: 10.3967/bes2024.107
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Original Article
Identifying Comprehensive Genomic Alterations and Potential Neoantigens for Cervical Cancer Immunotherapy in a Cohort of Chinese Squamous Cell Carcinoma of the Cervix
Meng Wu, Jialu Zhou, Zhe Zhang, Yuanguang Meng
2024, 37(6): 565-580.   doi: 10.3967/bes2024.064
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  Objective   Genomic alterations and potential neoantigens for cervical cancer immunotherapy were identified in a cohort of Chinese patients with cervical squamous cell carcinoma (CSCC).   Methods   Whole-exome sequencing was used to identify genomic alterations and potential neoantigens for CSCC immunotherapy. RNA Sequencing was performed to analyze neoantigen expression.   Results   Systematic bioinformatics analysis showed that C>T/G>A transitions/transversions were dominant in CSCCs. Missense mutations were the most frequent types of somatic mutation in the coding sequence regions. Mutational signature analysis detected signature 2, signature 6, and signature 7 in CSCC samples. PIK3CA, FBXW7, and BICRA were identified as potential driver genes, with BICRA as a newly reported gene. Genomic variation profiling identified 4,960 potential neoantigens, of which 114 were listed in two neoantigen-related databases.  Conclusion   The present findings contribute to our understanding of the genomic characteristics of CSCC and provide a foundation for the development of new biotechnology methods for individualized immunotherapy in CSCC.
Predicting the Prognosis and Immunotherapeutic Response of Triple-Negative Breast Cancer by Constructing a Prognostic Model Based on CD8+ T Cell-Related Immune Genes
Nani Li, Xiaoting Qiu, Jingsong Xue, Limu Yi, Mulan Chen, Zhijian Huang
2024, 37(6): 581-593.   doi: 10.3967/bes2024.065
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  Objective  Triple-negative breast cancer (TNBC) poses a significant challenge for treatment efficacy. CD8+ T cells, which are pivotal immune cells, can be effectively analyzed for differential gene expression across diverse cell populations owing to rapid advancements in sequencing technology. By leveraging these genes, our objective was to develop a prognostic model that accurately predicts the prognosis of patients with TNBC and their responsiveness to immunotherapy.  Methods  Sample information and clinical data of TNBC were sourced from The Cancer Genome Atlas and METABRIC databases. In the initial stage, we identified 67 differentially expressed genes associated with immune response in CD8+ T cells. Subsequently, we narrowed our focus to three key genes, namely CXCL13, GBP2, and GZMB, which were used to construct a prognostic model. The accuracy of the model was assessed using the validation set data and receiver operating characteristic (ROC) curves. Furthermore, we employed various methods, including Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, immune infiltration, and correlation analyses with CD274 (PD-L1) to explore the model's predictive efficacy in immunotherapeutic responses. Additionally, we investigated the potential underlying biological pathways that contribute to divergent treatment responses.  Results  We successfully developed a model capable of predicting the prognosis of patients with TNBC. The areas under the curve (AUC) values for the 1-, 3-, and 5-year survival predictions were 0.618, 0.652, and 0.826, respectively. Employing this risk model, we stratified the samples into high- and low-risk groups. Through KEGG enrichment analysis, we observed that the high-risk group predominantly exhibited enrichment in metabolism-related pathways such as drug and chlorophyll metabolism, whereas the low-risk group demonstrated significant enrichment in cytokine pathways. Furthermore, immune landscape analysis revealed noteworthy variations between (PD-L1) expression and risk scores, indicating that our model effectively predicted the response of patients to immune-based treatments.  Conclusion  Our study demonstrates the potential of CXCL13, GBP2, and GZMB as prognostic indicators of clinical outcomes and immunotherapy responses in patients with TNBC. These findings provide valuable insights and novel avenues for developing immunotherapeutic approaches targeting TNBC.
Knockout of C6orf120 in Rats Alleviates Concanavalin A-induced Autoimmune Hepatitis by Regulating Macrophage Polarization
Xin Wang, Yuqi Wang, Hui Liu, Yingying Lin, Peng Wang, Yunyun Yi, Xin Li
2024, 37(6): 594-606.   doi: 10.3967/bes2024.066
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  Objective  The effect of the functionally unknown gene C6orf120 on autoimmune hepatitis was investigated on C6orf120 knockout rats (C6orf120-/-) and THP-1 cells.  Method  Six–eight-week-old C6orf120-/- and wild-type (WT) SD rats were injected with Con A (16 mg/kg), and euthanized after 24 h. The sera, livers, and spleens were collected. THP-1 cells and the recombinant protein (rC6ORF120) were used to explore the mechanism in vitro. The frequency of M1 and M2 macrophages was analyzed using flow cytometry. Western blotting and PCR were used to detect macrophage polarization-associated factors.  Results  C6orf120 knockout attenuated Con A-induced autoimmune hepatitis. Flow cytometry indicated that the proportion of CD68+CD86+M1 macrophages from the liver and spleen in the C6orf120-/- rats decreased. C6orf120 knockout induced downregulation of CD86 protein and the mRNA levels of related inflammatory factors TNF-α, IL-1β, and IL-6 in the liver. C6orf120 knockout did not affect the polarization of THP-1 cells. However, rC6ORF120 promoted the THP-1 cells toward CD68+CD80+M1 macrophages and inhibited the CD68+CD206+M2 phenotype.  Conclusion  C6orf120 knockout alleviates Con A-induced autoimmune hepatitis by inhibiting macrophage polarization toward M1 macrophages and reducing the expression of related inflammatory factors in C6orf120-/- rats.
The Association between GLP-1 Receptor-Based Agonists and the Incidence of Asthma in Patients with Type 2 Diabetes and/or Obesity: A Meta-Analysis
Mengqing Zhang, Chu Lin, Xiaoling Cai, Ruoyang Jiao, Shuzhen Bai, Zonglin Li, Suiyuan Hu, Fang Lyu, Wenjia Yang, Linong Ji
2024, 37(6): 607-616.   doi: 10.3967/bes2024.067
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  Objective  Recent studies have indicated potential anti-inflammatory effects of glucagon-like peptide-1 receptor agonists (GLP-1RAs) on asthma, which is often comorbid with type 2 diabetes mellitus (T2DM) and obesity. Therefore, we conducted a meta-analysis to assess the association between the administration of glucagon-like peptide-1 (GLP-1) receptor-based agonists and the incidence of asthma in patients with T2DM and/or obesity.  Methods  PubMed, Web of Science, Embase, the Cochrane Central Register of Controlled Trials, and Clinicaltrial.gov were systematically searched from inception to July 2023. Randomized controlled trials (RCTs) of GLP-1 receptor-based agonists (GLP-1RA, GLP-1 based dual and triple receptor agonist) with reports of asthma events were included. Outcomes were computed as risk ratios (RR) using a fixed-effects model.  Results  Overall, 39 RCTs with a total of 85,755 participants were included. Compared to non-GLP-1 receptor-based agonist users, a trend of reduced risk of asthma was observed in patients with T2DM or obesity using GLP-1 receptor-based agonist treatments, although the difference was not statistically significant [RR = 0.91, 95% confidence interval (CI): 0.68 to 1.24]. Further Subgroup analyses indicated that the use of light-molecular-weight GLP-1RAs might be associated with a reduced the risk of asthma when compared with non-users (RR = 0.65, 95% CI: 0.43 to 0.99, P = 0.043). We also performed sensitivity analyses for participant characteristics, study design, drug structure, duration of action, and drug subtypes. However, no significant associations were observed.  Conclusion  Compared with non-users, a modest reduction in the incidence of asthma was observed in patients with T2DM or obesity using GLP-1 receptor-based agonist treatments. Further investigations are warranted to assess the association between GLP-1 receptor-based agonists and the risk of asthma.
SiO2 Induces Iron Overload and Ferroptosis in Cardiomyocytes in a Silicosis Mouse Model
Yongheng Wang, Ning Li, Yi Guan, Tong LI, Yuxiu Zhang, Hong Cao, Zhihua Yu, Zhiheng Li, Shuoyan Li, Jiahao Hu, Wenxin Zhou, Sisi Qin, Shuang Li, Sanqiao Yao
2024, 37(6): 617-627.   doi: 10.3967/bes2024.087
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  Objective   The aim of this study was to explore the role and mechanism of ferroptosis in SiO2-induced cardiac injury using a mouse model.  Methods   Male C57BL/6 mice were intratracheally instilled with SiO2 to create a silicosis model. Ferrostatin-1 (Fer-1) and deferoxamine (DFO) were used to suppress ferroptosis. Serum biomarkers, oxidative stress markers, histopathology, iron content, and the expression of ferroptosis-related proteins were assessed.  Results   SiO2 altered serum cardiac injury biomarkers, oxidative stress, iron accumulation, and ferroptosis markers in myocardial tissue. Fer-1 and DFO reduced lipid peroxidation and iron overload, and alleviated SiO2-induced mitochondrial damage and myocardial injury. SiO2 inhibited Nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream antioxidant genes, while Fer-1 more potently reactivated Nrf2 compared to DFO.  Conclusion   Iron overload-induced ferroptosis contributes to SiO2-induced cardiac injury. Targeting ferroptosis by reducing iron accumulation or inhibiting lipid peroxidation protects against SiO2 cardiotoxicity, potentially via modulation of the Nrf2 pathway.
Antibody Levels and Infection Status of Pertussis in the Population under Pertussis Resurgence in Guangxi in 2018: A Cross-Sectional Survey
Liang Liang, Qiuyun Deng, Lili Deng, Jinghang Wei, Shiyi Chen, Yizhi Wei, Yuyan Ma, Yue Qin, Wei Liu
2024, 37(6): 628-638.   doi: 10.3967/bes2024.069
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  Objective  Pertussis cases have increased markedly since 2018 in Guangxi. The aim of this study was to evaluate antibody levels and the infection status of pertussis in the resident population.  Method  A total of 10,215 serum samples from residents were collected from August–November 2018 and tested for anti-pertussis IgG and toxin IgG using the enzyme-linked immunosorbent assay (ELISA).  Results  Of the collected samples, 1,833 (17.94%) tested positive for anti-pertussis IgG, with the median concentration of 16.06 IU/mL. Antibody level < 10 IU/mL accounted for more than 60% in children under 4 years of age, but declined with age, whereas the percentages of the other three levels (10–40, 40–50, and ≥ 50 IU/mL) increased almost with age (P < 0.001). Moreover, 7,924 samples were selected for anti-pertussis toxin IgG, of which 653 (8.24%) tested positive (≥ 40 IU/mL) with the median concentration of 5.89 IU/mL, and 204 participants (2.56%) had recent pertussis infection (≥ 100 IU/mL). Among the different age groups, the highest rates of positivity and recent infection were observed at 11–20 years of age, the lowest positivity rate at 5 years of age, and the lowest recent infection rate at 4 years of age (P < 0.001, P = 0.005, respectively).   Conclusion  The survey results showed that all age groups in Guangxi lacked immunity against pertussis, which was one of the main factors contributing to the resurgence of pertussis in 2018. In addition, the prevalence of pertussis is relatively high in Guangxi, and its incidence is seriously underestimated, especially in adolescents and adults.
Development of an Integrated Disposable Device for SARS-CoV-2 Nucleic Acid Extraction and Detection
Jing Ma, Yanzhe Hao, Meiling Hou, Xiaoshan Zhang, Jingduan Liu, Haodi Meng, Jiangbo Chang, Xuejun Ma, Jihua Liu, Qingjie Ying, Xianhua Wang, Hongxia Li, Yuxi Cao, Xiaoguang Zhang
2024, 37(6): 639-646.   doi: 10.3967/bes2024.070
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  Objective   To develop a highly sensitive and rapid nucleic acid detection method for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).  Methods   We designed, developed, and manufactured an integrated disposable device for SARS-CoV-2 nucleic acid extraction and detection. The precision of the liquid transfer and temperature control was tested. A comparison between our device and a commercial kit for SARS-Cov-2 nucleic acid extraction was performed using real-time fluorescence reverse transcription polymerase chain reaction (RT-PCR). The entire process, from SARS-CoV-2 nucleic acid extraction to amplification, was evaluated.  Results   The precision of the syringe transfer volume was 19.2 ± 1.9 μL (set value was 20), 32.2 ± 1.6 (set value was 30), and 57.2 ± 3.5 (set value was 60). Temperature control in the amplification tube was measured at 60.0 ± 0.0 °C (set value was 60) and 95.1 ± 0.2 °C (set value was 95) respectively. SARS-Cov-2 nucleic acid extraction yield through the device was 7.10 × 106 copies/mL, while a commercial kit yielded 2.98 × 106 copies/mL. The mean time to complete the entire assay, from SARS-CoV-2 nucleic acid extraction to amplification detection, was 36 min and 45 s. The detection limit for SARS-CoV-2 nucleic acid was 250 copies/mL.  Conclusion   The integrated disposable devices may be used for SARS-CoV-2 Point-of-Care test (POCT).
Review
Research Progress on the Association between Schizophrenia and Toxoplasma gondii Infection
Yiting Zhu, Xiaohui Yang, Miaoru Chen, Yu Hu, Yunfeng Chang, Xiang Wu
2024, 37(6): 647-660.   doi: 10.3967/bes2024.071
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Abstract: Toxoplasma gondii (T. gondii or Tg), is an obligatory intracellular parasite with humans as its intermediate hosts. In recent years, significant correlations between T. gondii infection and schizophrenia have been reported, including the possible mediating mechanisms. Currently, mechanisms and hypotheses focus on central neurotransmitters, immunity, neuroinflammation, and epigenetics; however, the exact underlying mechanisms remain unclear. In this article, we review the studies related to T. gondii infection and schizophrenia, particularly the latest research progress. Research on dopamine (DA) and other neurotransmitters, the blood-brain barrier, inflammatory factors, disease heterogeneity, and other confounders is also discussed. In addition, we also summarized the results of some new epidemiological investigations.
Letter to the Editor
Selenium, Type-2 Diabetes, and the Possible Protective Role of Vitamin D
Runxue Ma, Ce Liu, Ling Zhang, Jingzhe Guo, Erkai Zhou, Ling Zheng, Li He, Xiaobing Shan, Yunhui Yu, Bin Luo
2024, 37(6): 661-665.   doi: 10.3967/bes2024.072
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Associations of Perchlorate, Nitrate, and Thiocyanate with Sex Hormones among Children: A Nationally Representative Cross-sectional Study in U.S.
Yiwen Wang, Guodong Ding, Peipei Hu, Yongjun Zhang
2024, 37(6): 666-671.   doi: 10.3967/bes2024.073
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Genetically Predicted Plasma Levels of Amino Acids and Endometriosis: A Mendelian Randomization Study
Juanmei Li, Yao Ni, Ning Wang, Yu Liao, Yiwei Yu, Wenliang Lyu, Ruihua Zhao
2024, 37(6): 672-675.   doi: 10.3967/bes2024.074
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Inhibition of the NF-κB Signaling Pathway Improves Cigarette Mainstream Smoke-Induced Lung Injury and Gut Microbiota Disturbance
Hong Huang, Shengjie Li, Lifang Zeng, Yan Zhang, Ying Chen, Yanbing Ma, Jing Wei, Changwei Zou, Tingtao Chen
2024, 37(6): 676-681.   doi: 10.3967/bes2024.075
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Health Effects of Extreme Low Temperatures and Cold Waves on Respiratory Diseases
Zongrui Liu, Yuxia Ma, Yuhan Zhao, Wanci Wang, Pengpeng Qin, Jie Yang, Bowen Cheng
2024, 37(6): 682-685.   doi: 10.3967/bes2024.076
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Bone Injury and Fracture Healing Biology
Ahmad Oryan, Somayeh Monazzah, Amin Bigham-Sadegh
2015, 28(1): 57-71.   doi: 10.3967/bes2015.006
[Abstract](10340) [PDF 3875KB](4367)
Report on Cardiovascular Health and Diseases in China 2021: An Updated Summary
The Writing Committee of the Report on Cardiovascular Health and Diseases in China
2022, 35(7): 573-603.   doi: 10.3967/bes2022.079
[Abstract](11789) [FullText HTML](5575) [PDF 2336KB](5575)
The Serum Exosome Derived MicroRNA-135a, -193b, and-384 Were Potential Alzheimer's Disease Biomarkers
YANG Ting Ting, LIU Chen Geng, GAO Shi Chao, ZHANG Yi, WANG Pei Chang
2018, 31(2): 87-96.   doi: 10.3967/bes2018.011
[Abstract](7677) [FullText HTML](2592) [PDF 11333KB](2592)
Burden of Cirrhosis and Other Chronic Liver Diseases Caused by Specific Etiologies in China, 1990−2016: Findings from the Global Burden of Disease Study 2016
LI Man, WANG Zhuo Qun, ZHANG Lu, ZHENG Hao, LIU Dian Wu, ZHOU Mai Geng
2020, 33(1): 1-10.   doi: 10.3967/bes2020.001
[Abstract](8195) [FullText HTML](2891) [PDF 2552KB](2891)
Effects of Short-Term Forest Bathing on Human Health in a Broad-Leaved Evergreen Forest in Zhejiang Province, China
MAO Gen Xiang, LAN Xiao Guang, CAO Yong Bao, CHEN Zhuo Mei, HE Zhi Hua, LV Yuan Dong, WANG Ya Zhen, HU Xi Lian, WANG Guo Fu, YAN Jing
2012, 25(3): 317-324.   doi: 10.3967/0895-3988.2012.03.010
[Abstract](5529) [PDF 528KB](2254)
Trends in Lipids Level and Dyslipidemia among Chinese Adults, 2002-2015
SONG Peng Kun, MAN Qing Qing, LI Hong, PANG Shao Jie, JIA Shan Shan, LI Yu Qian, HE Li, ZHAO Wen Hua, ZHANG Jian
2019, 32(8): 559-570.   doi: 10.3967/bes2019.074
[Abstract](5815) [FullText HTML](2000) [PDF 3641KB](2000)
Stability of SARS Coronavirus in Human Specimens and Environment and Its Sensitivity to Heating and UV Irradiation
SHU-MING DUAN, Xin-sheng Zhao, RUI-FU WEN, JING-JING HUANG, GUO-HUA PI, SU-XIANG ZHANG, JUN HAN, SHENG-LI BI, LI RUAN, XIAO-PING DONG, SARS RESEARCH TEAM
2003, 16(3): 246-255.  
[Abstract](7046) [PDF 610KB](1717)
Protein Requirements in Healthy Adults:A Meta-analysis of Nitrogen Balance Studies
LI Min, SUN Feng, PIAO Jian Hua, YANG Xiao Guang
2014, 27(8): 606-613.   doi: 10.3967/bes2014.093
[Abstract](4097) [PDF 8784KB](1572)
Metabolomic Profiling Differences among Asthma, COPD, and Healthy Subjects: A LC-MS-based Metabolomic Analysis
LIANG Ying, GAI Xiao Yan, CHANG Chun, ZHANG Xu, WANG Juan, LI Ting Ting
2019, 32(9): 659-672.   doi: 10.3967/bes2019.085
[Abstract](4921) [FullText HTML](1430) [PDF 2914KB](1430)
Report on Cardiovascular Health and Diseases in China 2022: an Updated Summary
The Writing Committee of the Report on Cardiovascular Health and Diseases in China
2023, 36(8): 669-701.   doi: 10.3967/bes2023.106
[Abstract](3644) [FullText HTML](1779) [PDF 1561KB](1779)
TaqMan Real-time RT-PCR Assay for Detecting and Differentiating Japanese Encephalitis Virus
SHAO Nan, LI Fan, NIE Kai, FU Shi Hong, ZHANG Wei Jia, HE Ying, LEI Wen Wen, WANG Qian Ying, LIANG Guo Dong, CAO Yu Xi, WANG Huan Yu
2018, 31(3): 208-214.   doi: 10.3967/bes2018.026
[Abstract](4147) [FullText HTML](1585) [PDF 4691KB](1585)
Evaluating the Nutritional Status of Oncology Patientsand Its Association with Quality of Life
ZHANG Ya Hui, XIE Fang Yi, CHEN Ya Wen, WANG Hai Xia, TIAN Wen Xia, SUN Wen Guang, WU Jing
2018, 31(9): 637-644.   doi: 10.3967/bes2018.088
[Abstract](5514) [FullText HTML](1525) [PDF 31943KB](1525)
Evidence on Invasion of Blood, Adipose Tissues, Nervous System and Reproductive System of Mice After a Single Oral Exposure: Nanoplastics versus Microplastics
YANG Zuo Sen, BAI Ying Long, JIN Cui Hong, NA Jun, ZHANG Rui, GAO Yuan, PAN Guo Wei, YAN Ling Jun, SUN Wei
2022, 35(11): 1025-1037.   doi: 10.3967/bes2022.131
[Abstract](2348) [FullText HTML](1115) [PDF 10064KB](1115)
Health Effect of Forest Bathing Trip on Elderly Patients with Chronic Obstructive Pulmonary Disease
JIA Bing Bing, YANG Zhou Xin, MAO Gen Xiang, LYU Yuan Dong, WEN Xiao Lin, XU Wei Hong, LYU XIAO Ling
2016, 29(3): 212-218.   doi: 10.3967/bes2016.026
[Abstract](2637) [PDF 803KB](1017)
Supplementation of Fermented Barley Extracts with Lactobacillus Plantarum dy-1 Inhibits Obesity via a UCP1-dependent Mechanism
XIAO Xiang, BAI Juan, LI Ming Song, ZHANG Jia Yan, SUN Xin Juan, DONG Ying
2019, 32(8): 578-591.   doi: 10.3967/bes2019.076
[Abstract](4745) [FullText HTML](1146) [PDF 9101KB](1146)
Application of Nanopore Sequencing Technology in the Clinical Diagnosis of Infectious Diseases
ZHANG Lu Lu, ZHANG Chi, PENG Jun Ping
2022, 35(5): 381-392.   doi: 10.3967/bes2022.054
[Abstract](2668) [FullText HTML](1377) [PDF 2174KB](1377)
Protective Effect of Angiotensin (1-7) on Silicotic Fibrosis in Rats
ZHANG Bo Nan, XU Hong, GAO Xue Min, ZHANG Gui Zhen, ZHANG Xin, YANG Fang
2019, 32(6): 419-426.   doi: 10.3967/bes2019.057
[Abstract](3555) [PDF 1328KB](977)
The Emergence, Epidemiology, and Etiology of Haff Disease
PEI Pei, LI Xiao Yan, LU Shuang Shuang, LIU Zhe, WANG Rui, LU Xuan Cheng, LU Kai
2019, 32(10): 769-778.   doi: 10.3967/bes2019.096
[Abstract](4624) [FullText HTML](1913) [PDF 2249KB](1913)
Hypertension Prevalence, Awareness, Treatment, and Control and Their Associated Socioeconomic Factors in China: A Spatial Analysis of A National Representative Survey
WANG Wei, ZHANG Mei, XU Cheng Dong, YE Peng Peng, LIU Yun Ning, HUANG Zheng Jing, HU Cai Hong, ZHANG Xiao, ZHAO Zhen Ping, LI Chun, CHEN Xiao Rong, WANG Li Min, ZHOU Mai Geng
2021, 34(12): 937-951.   doi: 10.3967/bes2021.130
[Abstract](2748) [FullText HTML](1318) [PDF 2205KB](1318)
Mutual Impact of Diabetes Mellitus and Tuberculosis in China
CHENG Jun, ZHANG Hui, ZHAO Yan Lin, WANG Li Xia, CHEN Ming Ting
2017, 30(5): 384-389.   doi: 10.3967/bes2017.051
[Abstract](3929) [FullText HTML](1345) [PDF 671KB](1345)

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Vol 37, No 6

(June, 2024)

ISSN 0895-3988

CN 11-2816/Q

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