Volume 14 Issue 3
Sep.  2001
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YOGESHWER SHUKLA. Histochemical Localization of Glutathione Dependent NBT-Reductase in Mouse Skin[J]. Biomedical and Environmental Sciences, 2001, 14(3): 237-240.
Citation: YOGESHWER SHUKLA. Histochemical Localization of Glutathione Dependent NBT-Reductase in Mouse Skin[J]. Biomedical and Environmental Sciences, 2001, 14(3): 237-240.

Histochemical Localization of Glutathione Dependent NBT-Reductase in Mouse Skin

  • Objective Localization of the glutathione dependent Nitroblue tetrazolium (NBT) reductase in fresh frozen sections of mouse skin and possible dependence of NBT reductase on tissue thiol levels has been investigated. Methods The fresh frozen tissue sections (8m thickness) were prepared and incubated in medium containing NBT, reduced glutathione (GSH) and phosphate buffer. The staining for GSH was performed with mercury orange. Results  The activity of the NBT-reductase in mouse skin has been found to be localized in the areas rich in glutathione and actively proliferating area of the skin. Conclusion The activity of the NBT-reductase seems to be dependent on the glutathione contents.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Histochemical Localization of Glutathione Dependent NBT-Reductase in Mouse Skin

Abstract: Objective Localization of the glutathione dependent Nitroblue tetrazolium (NBT) reductase in fresh frozen sections of mouse skin and possible dependence of NBT reductase on tissue thiol levels has been investigated. Methods The fresh frozen tissue sections (8m thickness) were prepared and incubated in medium containing NBT, reduced glutathione (GSH) and phosphate buffer. The staining for GSH was performed with mercury orange. Results  The activity of the NBT-reductase in mouse skin has been found to be localized in the areas rich in glutathione and actively proliferating area of the skin. Conclusion The activity of the NBT-reductase seems to be dependent on the glutathione contents.

YOGESHWER SHUKLA. Histochemical Localization of Glutathione Dependent NBT-Reductase in Mouse Skin[J]. Biomedical and Environmental Sciences, 2001, 14(3): 237-240.
Citation: YOGESHWER SHUKLA. Histochemical Localization of Glutathione Dependent NBT-Reductase in Mouse Skin[J]. Biomedical and Environmental Sciences, 2001, 14(3): 237-240.

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