Volume 17 Issue 1
Mar.  2004
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JIAN-MING JIANG, Zhen Wang, DIAN-DONG LI. Effects of AGEs on Oxidation Stress and Antioxidation Abilities in Cultured Astrocytes[J]. Biomedical and Environmental Sciences, 2004, 17(1): 79-86.
Citation: JIAN-MING JIANG, Zhen Wang, DIAN-DONG LI. Effects of AGEs on Oxidation Stress and Antioxidation Abilities in Cultured Astrocytes[J]. Biomedical and Environmental Sciences, 2004, 17(1): 79-86.

Effects of AGEs on Oxidation Stress and Antioxidation Abilities in Cultured Astrocytes

Funds:  This work was supported by a grant from the National Key Basic Research Development Program Foundation of China(G2000057010)%国家自然科学基金(30070827)
  • Objective To investigate whether two kinds of in vitro prepared advanced glycation end products (AGEs), Glu-BSA and Gal-BSA, could change oxidation stress and anti-oxidation abilities in astrocytes, and thus might contribute to brain injury. Methods Changes of GSH, MDA, SOD,MAO-B, nitric oxide were measured after AGEs treatment. Results Both 0.1 g/L Glu-BSA and Gal-BSA could slightly decrease GSH level, while 1 g/L of them significantly decreased GSH level by 35% and 43% respectively. The MDA levels of both 1 g/L AGEs treated groups (306±13 and 346±22) were higher than that of the normal group (189±18), which could be inhibited by free radical scavenger NAC. The SOD activities of both 1 g/L AGEs treated groups (67.0±5.2 and 74.0±11.0)were lower than that of the normal group (85.2±8.0). Both 0.1 g/L AGEs could slightly increase the activity of MAO-B, while 1 g/L of them could increase MAO-B activity by 1.5 and 1.7 folds respectively. Both AGEs stimulation could produce NO level by 1.7 and 2 folds respectively.Conclusion Enhanced levels of astrocytic oxidation stress and decrease of antioxidation abilities may contribute to, at least partially, the detrimental effects of AGEs in neuronal disorders and aging brain.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Effects of AGEs on Oxidation Stress and Antioxidation Abilities in Cultured Astrocytes

Funds:  This work was supported by a grant from the National Key Basic Research Development Program Foundation of China(G2000057010)%国家自然科学基金(30070827)

Abstract: Objective To investigate whether two kinds of in vitro prepared advanced glycation end products (AGEs), Glu-BSA and Gal-BSA, could change oxidation stress and anti-oxidation abilities in astrocytes, and thus might contribute to brain injury. Methods Changes of GSH, MDA, SOD,MAO-B, nitric oxide were measured after AGEs treatment. Results Both 0.1 g/L Glu-BSA and Gal-BSA could slightly decrease GSH level, while 1 g/L of them significantly decreased GSH level by 35% and 43% respectively. The MDA levels of both 1 g/L AGEs treated groups (306±13 and 346±22) were higher than that of the normal group (189±18), which could be inhibited by free radical scavenger NAC. The SOD activities of both 1 g/L AGEs treated groups (67.0±5.2 and 74.0±11.0)were lower than that of the normal group (85.2±8.0). Both 0.1 g/L AGEs could slightly increase the activity of MAO-B, while 1 g/L of them could increase MAO-B activity by 1.5 and 1.7 folds respectively. Both AGEs stimulation could produce NO level by 1.7 and 2 folds respectively.Conclusion Enhanced levels of astrocytic oxidation stress and decrease of antioxidation abilities may contribute to, at least partially, the detrimental effects of AGEs in neuronal disorders and aging brain.

JIAN-MING JIANG, Zhen Wang, DIAN-DONG LI. Effects of AGEs on Oxidation Stress and Antioxidation Abilities in Cultured Astrocytes[J]. Biomedical and Environmental Sciences, 2004, 17(1): 79-86.
Citation: JIAN-MING JIANG, Zhen Wang, DIAN-DONG LI. Effects of AGEs on Oxidation Stress and Antioxidation Abilities in Cultured Astrocytes[J]. Biomedical and Environmental Sciences, 2004, 17(1): 79-86.

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