Effect of X-rays on Expression of Caspase-3 and p53 in EL-4 Cells and Its Biological Implications

GUI-ZHI JU; BO SHEN; SHI-LONG SUN; FENG-QIN YAN; SHI-BO FU

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Volume 20 Issue 6

Dec. 2007

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Article Navigation > Biomedical and Environmental Sciences > 2007 > 20(6): 456-459

GUI-ZHI JU, BO SHEN, SHI-LONG SUN, FENG-QIN YAN, SHI-BO FU. Effect of X-rays on Expression of Caspase-3 and p53 in EL-4 Cells and Its Biological Implications[J]. Biomedical and Environmental Sciences, 2007, 20(6): 456-459.

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GUI-ZHI JU, BO SHEN, SHI-LONG SUN, FENG-QIN YAN, SHI-BO FU. Effect of X-rays on Expression of Caspase-3 and p53 in EL-4 Cells and Its Biological Implications[J]. Biomedical and Environmental Sciences, 2007, 20(6): 456-459.

Effect of X-rays on Expression of Caspase-3 and p53 in EL-4 Cells and Its Biological Implications

Department of Radiation Biology, School of Public Health, Jilin University,Changchun 130021, Jilin, China

Funds: 国家自然科学基金(30670630)

Abstract

Objective To investigate the effect of X-rays on expression of caspase-3 and p53 protein in EL-4 cells and its implications in induction of apoptosis and polyploid cells. Methods Mouse lymphoma cell line (EL-4 cells) was used.Fluorescent staining and flow cytometry analysis were employed for measurement of protein expression, apoptosis, cell cycle,and polyploid cells. Results The expression of caspase-3 protein increased significantly at 8 h and 12 h, compared with that of sham-irradiated control (P＜0.05, respectively) and the expression of p53 protein increased significantly at 2, 4, 8, 12, and 24 h, compared with that of sham-irradiated control (P＜0.05-P＜0.01) in EL-4 cells after 4.0 Gy X-irradiation. Apoptosis of EL-4 cells was increased significantly at 2, 4, 8, 12, 24, 48, and 72 h after 4.0Gy exposure, compared with that of sham-irradiated control (P＜0.05-P＜0.001). G2 phase cells were increased significantly at 4, 8, 12, 24, 48, and 72 h (P＜0.05-P＜0.001). However, no marked change in the number of 8 C polyploid cells was found from 2 to 48 h after 4.0 Gy exposure. Conclusion The expressions of caspase-3 and p53 protein in EL-4 cells are induced by X-rays, which might play an important role in the induction of apoptosis, and the molecular pathway for polyploid formation might be p53-independent.
- X-rays,
- Caspase-3,
- p53,
- Apoptosis,
- Polyploid
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Effect of X-rays on Expression of Caspase-3 and p53 in EL-4 Cells and Its Biological Implications

Department of Radiation Biology, School of Public Health, Jilin University,Changchun 130021, Jilin, China

Funds: 国家自然科学基金(30670630)

Key words:

X-rays /
Caspase-3 /
p53 /
Apoptosis /
Polyploid

Abstract: Objective To investigate the effect of X-rays on expression of caspase-3 and p53 protein in EL-4 cells and its implications in induction of apoptosis and polyploid cells. Methods Mouse lymphoma cell line (EL-4 cells) was used.Fluorescent staining and flow cytometry analysis were employed for measurement of protein expression, apoptosis, cell cycle,and polyploid cells. Results The expression of caspase-3 protein increased significantly at 8 h and 12 h, compared with that of sham-irradiated control (P＜0.05, respectively) and the expression of p53 protein increased significantly at 2, 4, 8, 12, and 24 h, compared with that of sham-irradiated control (P＜0.05-P＜0.01) in EL-4 cells after 4.0 Gy X-irradiation. Apoptosis of EL-4 cells was increased significantly at 2, 4, 8, 12, 24, 48, and 72 h after 4.0Gy exposure, compared with that of sham-irradiated control (P＜0.05-P＜0.001). G2 phase cells were increased significantly at 4, 8, 12, 24, 48, and 72 h (P＜0.05-P＜0.001). However, no marked change in the number of 8 C polyploid cells was found from 2 to 48 h after 4.0 Gy exposure. Conclusion The expressions of caspase-3 and p53 protein in EL-4 cells are induced by X-rays, which might play an important role in the induction of apoptosis, and the molecular pathway for polyploid formation might be p53-independent.

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