Reversal of Apoptotic Resistance by Lycium barbarum Glycopeptide 3 in Aged T Cells
Abstract: Objective To study whether Lycium barbarian glycopeptide 3 (LBGP3) affects T cell apeptosis in aged mice. Methods LBGP3 was purified with DEAE cellulose and Sephadex columns. Apoptotic "sub-Gl peak" was detected by flow cytometry and DNA ladder was resolved by agarose gel electrophoresis. Levels of IFN-γ, and IL-10 were measured with specific kits and mRNA expression was detected by RT-PCR. Apoptosis-related proteins of FLIP, FasL, and Bcl-2 were determined by Western blotting. Resdts LBGP3 was purified from Fructus Lycii water extracts and identified as a 41 kD glycopeptide.Treatment with 200 μg/mL LBGP3 increased the apoptotic rate of T cells from aged mice and showed a similar DNA ladder pattern to that in young T ceils. The reversal of apoptotic resistance was involved in down-regulating the expression of Bcl-2 and FLIP, and up-regulating the expression of FasL. Conclusion Lycium barbarum glycopeptide 3 reverses apoptotic resistance of aged T cells by modulating the expression of apoptosis-related molecules.
|Citation:||LONG-GUO YUAN, HONG-BIN DENG, LI-HUI CHEN, DIAN-DONG LI, QI-YANG HE. Reversal of Apoptotic Resistance by Lycium barbarum Glycopeptide 3 in Aged T Cells[J]. Biomedical and Environmental Sciences, 2008, 21(3): 212-217.|