Volume 19 Issue 1
Feb.  2006
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GUI-DONG DAI, LUN-BIAO CUI, LING SONG, REN-ZHEN ZHAO, JIAN-FENG CHEN, YU-BANG WANG, HEBRON C. CHANG, XIN-RU WANG. Metabolism of Terephthalic Acid and Its Effects on CYP4B1 Induction[J]. Biomedical and Environmental Sciences, 2006, 19(1): 8-14.
Citation: GUI-DONG DAI, LUN-BIAO CUI, LING SONG, REN-ZHEN ZHAO, JIAN-FENG CHEN, YU-BANG WANG, HEBRON C. CHANG, XIN-RU WANG. Metabolism of Terephthalic Acid and Its Effects on CYP4B1 Induction[J]. Biomedical and Environmental Sciences, 2006, 19(1): 8-14.

Metabolism of Terephthalic Acid and Its Effects on CYP4B1 Induction

Funds:  the National Natural Science Foundation of China(30170798)%the Preliminary Study of an Important Project in the National Basic Research(2001CCA04900)%the Greatest Project in the National Basic Research(2002CB512908)
  • Objective To investgate the metabolism of terephthalic acid (TPA) in rats and its mechanism. Methods Metabolism was evaluated by incubating sodium terephthalate (NaTPA) with rat normal liver microsomes, or with microsomes pretreated by phenobarbital sodium, or with 3-methycholanthrene, or with diet control following a NADPH-generating system. The determination was performed by high performance liquid chromatography (HPLC), and the mutagenic activation was analyzed by umu tester strain Salmonella typhimurium NM2009. Expression of CYP4B 1 mRNA was detected by RT-PCR. Results The amount of NaTPA (12.5-200 μmol· L-1) detected by HPLC did not decrease in microsomes induced by NADPH-generating system. Incubation of TPA (0.025-0.1 mmol·L-1) with induced or noninduced liver microsomes in an NM2009 umu response system did not show any mutagenic activation. TPA exposure increased the expression of CYP4B 1 mRNA in rat liver, kidney, and bladder. Conclusion Lack of metabolism of TPA in liver and negative genotoxic data from NM2009 study are consistent with other previous short-term tests, suggesting that the carcinogenesis in TPA feeding animals is not directly interfered with TPA itself and/or its metabolites.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Metabolism of Terephthalic Acid and Its Effects on CYP4B1 Induction

Funds:  the National Natural Science Foundation of China(30170798)%the Preliminary Study of an Important Project in the National Basic Research(2001CCA04900)%the Greatest Project in the National Basic Research(2002CB512908)

Abstract: Objective To investgate the metabolism of terephthalic acid (TPA) in rats and its mechanism. Methods Metabolism was evaluated by incubating sodium terephthalate (NaTPA) with rat normal liver microsomes, or with microsomes pretreated by phenobarbital sodium, or with 3-methycholanthrene, or with diet control following a NADPH-generating system. The determination was performed by high performance liquid chromatography (HPLC), and the mutagenic activation was analyzed by umu tester strain Salmonella typhimurium NM2009. Expression of CYP4B 1 mRNA was detected by RT-PCR. Results The amount of NaTPA (12.5-200 μmol· L-1) detected by HPLC did not decrease in microsomes induced by NADPH-generating system. Incubation of TPA (0.025-0.1 mmol·L-1) with induced or noninduced liver microsomes in an NM2009 umu response system did not show any mutagenic activation. TPA exposure increased the expression of CYP4B 1 mRNA in rat liver, kidney, and bladder. Conclusion Lack of metabolism of TPA in liver and negative genotoxic data from NM2009 study are consistent with other previous short-term tests, suggesting that the carcinogenesis in TPA feeding animals is not directly interfered with TPA itself and/or its metabolites.

GUI-DONG DAI, LUN-BIAO CUI, LING SONG, REN-ZHEN ZHAO, JIAN-FENG CHEN, YU-BANG WANG, HEBRON C. CHANG, XIN-RU WANG. Metabolism of Terephthalic Acid and Its Effects on CYP4B1 Induction[J]. Biomedical and Environmental Sciences, 2006, 19(1): 8-14.
Citation: GUI-DONG DAI, LUN-BIAO CUI, LING SONG, REN-ZHEN ZHAO, JIAN-FENG CHEN, YU-BANG WANG, HEBRON C. CHANG, XIN-RU WANG. Metabolism of Terephthalic Acid and Its Effects on CYP4B1 Induction[J]. Biomedical and Environmental Sciences, 2006, 19(1): 8-14.

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