Volume 21 Issue 4
Aug.  2008
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YIX-IONG LEI, LIAN WEI, MIN WANG, GEN-RONG WU, MIN LI. Malignant Transformation and Abnormal Expression of Eukaryotic Initiation Factor in Bronchial Epithelial Cells Induced by Cadmium Chloride[J]. Biomedical and Environmental Sciences, 2008, 21(4): 332-338.
Citation: YIX-IONG LEI, LIAN WEI, MIN WANG, GEN-RONG WU, MIN LI. Malignant Transformation and Abnormal Expression of Eukaryotic Initiation Factor in Bronchial Epithelial Cells Induced by Cadmium Chloride[J]. Biomedical and Environmental Sciences, 2008, 21(4): 332-338.

Malignant Transformation and Abnormal Expression of Eukaryotic Initiation Factor in Bronchial Epithelial Cells Induced by Cadmium Chloride

Funds:  国家自然科学基金(30371195)%广东省自然科学基金(06022672)%Guangzhou Science and Technology Foundation(2003Z2-E0191/E0192)%Guangzhou Municipal Department of Education(1002)
  • To analyze the relationship between malignant transformation and abnormal expression of eukaryotic initiation factor 3 (eIF3 p36) in human bronchial epithelial (16HBE) cells induced by cadmium chloride (CdCl<,2>). Methods 16HBE cellswere treated several dmes with different concentrations of CdCl<,2>. Tumorigenic potential of transformed cells was identified by assays for anchorage-independent growth in soft agar and for tumorigenicity in nude mice after the 35th passage. Total RNA was isolated from 16HBE cells induced by CdCl<,2>, including non-transformed, Cd-transformed, and Cd-tumorigenic cell lines. Special primers for eIF3 p36 were designed and the expression of eIF3 mRNA in different cell lines was detected with fluorescent quantitative-polymerase chain reaction technique (FQ-PCR). Results The 35th passage of 16HBE cells transformed by CdCl<,2> exhibited overlapping growth. Compared with the non-transformed cells, colonies of transformed cell lines in soft agar showed statistically significant increases and dose-dependent effects (P<0.01). All Cd-induced transformed cell lines formed tumors in nude mice within 2 weeks of inoculation, but none of the mice injected with non-transformed cells showed tumors even after 3 weeks. All tumors were pathologically identified as poorly differentiated squamous cell carcinoma. The eIF3 p36 genes in different stages of 16HBE cells transformed by CdCl<,2> were elevated as compared with the non-transformed control (P<0.01), and the eIF3 expression increased with the degree of cell malignancy. Conclusion CdCl<,2> is capable of inducing morphological transformation in 16HBE cells and transformed cells are potentially tumorigenic. Over-expression of eIF3 p36 is positively correlated with malignant transformation of 16HBE cells induced by CdCl<,2> and may be one of the molecular mechanisms potentially responsible for carcinogenesis due to Cd.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Malignant Transformation and Abnormal Expression of Eukaryotic Initiation Factor in Bronchial Epithelial Cells Induced by Cadmium Chloride

Funds:  国家自然科学基金(30371195)%广东省自然科学基金(06022672)%Guangzhou Science and Technology Foundation(2003Z2-E0191/E0192)%Guangzhou Municipal Department of Education(1002)

Abstract: To analyze the relationship between malignant transformation and abnormal expression of eukaryotic initiation factor 3 (eIF3 p36) in human bronchial epithelial (16HBE) cells induced by cadmium chloride (CdCl<,2>). Methods 16HBE cellswere treated several dmes with different concentrations of CdCl<,2>. Tumorigenic potential of transformed cells was identified by assays for anchorage-independent growth in soft agar and for tumorigenicity in nude mice after the 35th passage. Total RNA was isolated from 16HBE cells induced by CdCl<,2>, including non-transformed, Cd-transformed, and Cd-tumorigenic cell lines. Special primers for eIF3 p36 were designed and the expression of eIF3 mRNA in different cell lines was detected with fluorescent quantitative-polymerase chain reaction technique (FQ-PCR). Results The 35th passage of 16HBE cells transformed by CdCl<,2> exhibited overlapping growth. Compared with the non-transformed cells, colonies of transformed cell lines in soft agar showed statistically significant increases and dose-dependent effects (P<0.01). All Cd-induced transformed cell lines formed tumors in nude mice within 2 weeks of inoculation, but none of the mice injected with non-transformed cells showed tumors even after 3 weeks. All tumors were pathologically identified as poorly differentiated squamous cell carcinoma. The eIF3 p36 genes in different stages of 16HBE cells transformed by CdCl<,2> were elevated as compared with the non-transformed control (P<0.01), and the eIF3 expression increased with the degree of cell malignancy. Conclusion CdCl<,2> is capable of inducing morphological transformation in 16HBE cells and transformed cells are potentially tumorigenic. Over-expression of eIF3 p36 is positively correlated with malignant transformation of 16HBE cells induced by CdCl<,2> and may be one of the molecular mechanisms potentially responsible for carcinogenesis due to Cd.

YIX-IONG LEI, LIAN WEI, MIN WANG, GEN-RONG WU, MIN LI. Malignant Transformation and Abnormal Expression of Eukaryotic Initiation Factor in Bronchial Epithelial Cells Induced by Cadmium Chloride[J]. Biomedical and Environmental Sciences, 2008, 21(4): 332-338.
Citation: YIX-IONG LEI, LIAN WEI, MIN WANG, GEN-RONG WU, MIN LI. Malignant Transformation and Abnormal Expression of Eukaryotic Initiation Factor in Bronchial Epithelial Cells Induced by Cadmium Chloride[J]. Biomedical and Environmental Sciences, 2008, 21(4): 332-338.

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