Yeast One-hybrid System Used to Identify the Binding Proteins for Rat Glutathione S-transferase P Enhancer I

LIAO MING-XIANG; LIU DONG-YUAN; ZUO JIN; FANG FU-DE

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Volume 15 Issue 1

Mar. 2002

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Article Navigation > Biomedical and Environmental Sciences > 2002 > 15(1): 36-40

LIAO MING-XIANG, LIU DONG-YUAN, ZUO JIN, FANG FU-DE. Yeast One-hybrid System Used to Identify the Binding Proteins for Rat Glutathione S-transferase P Enhancer I[J]. Biomedical and Environmental Sciences, 2002, 15(1): 36-40.

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LIAO MING-XIANG, LIU DONG-YUAN, ZUO JIN, FANG FU-DE. Yeast One-hybrid System Used to Identify the Binding Proteins for Rat Glutathione S-transferase P Enhancer I[J]. Biomedical and Environmental Sciences, 2002, 15(1): 36-40.

Yeast One-hybrid System Used to Identify the Binding Proteins for Rat Glutathione S-transferase P Enhancer I

Institute of laboratory Animal Science, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100005, China

Funds: 国家自然科学基金(Grant 39670173,30170441)%国家高技术研究发展计划(863计划)(Grant 2001 AA221161)%北京市自然科学基金(7002026)%高等教育科研项目(20010023024)

Abstract

To detect the trans-factors specifically binding to the strong enhancer element (GPEI) in the upstream of rat glutathione S-transferase P (GST-P) gene. Methods Yeast one-hybrid system was used to screen rat lung MATCHMAKER cDNA library to identify potential trans-factors that can interact with core sequence of GPEI(cGPEI).Electrophoresis mobility shift assay (EMSA) was used to analyze the binding of transfactors to cGPEI. Results cDNA fragments coding for the C-terminal part of the transcription factor c-Jun and rat adenine nucleotide translocator (ANT) were isolated, The binding of c-Jun and ANT to GPEI core sequence were confirmed. Conclusions Rat c-jun transcriptional factor and ANT may interact with cGPEI. They could play an important role in the induced expression of GST-P gene.
- Glutathione S-transferase P enhancer I: Yeast one-hybrid system trans-action factor
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Yeast One-hybrid System Used to Identify the Binding Proteins for Rat Glutathione S-transferase P Enhancer I

Institute of laboratory Animal Science, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100005, China

Key words:

Glutathione S-transferase P enhancer I: Yeast one-hybrid system trans-action factor

Abstract: To detect the trans-factors specifically binding to the strong enhancer element (GPEI) in the upstream of rat glutathione S-transferase P (GST-P) gene. Methods Yeast one-hybrid system was used to screen rat lung MATCHMAKER cDNA library to identify potential trans-factors that can interact with core sequence of GPEI(cGPEI).Electrophoresis mobility shift assay (EMSA) was used to analyze the binding of transfactors to cGPEI. Results cDNA fragments coding for the C-terminal part of the transcription factor c-Jun and rat adenine nucleotide translocator (ANT) were isolated, The binding of c-Jun and ANT to GPEI core sequence were confirmed. Conclusions Rat c-jun transcriptional factor and ANT may interact with cGPEI. They could play an important role in the induced expression of GST-P gene.

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