ZHANG XiaoPeng; XIANG Qian; CUI WenMing; JIA XuDong; LI Ning

doi:10.3967/0895-3988.2011.04.013

Evaluation of the in vitro and in vivo Genotoxicity of Almond Skins

ZHANG XiaoPeng, XIANG Qian, CUI WenMing, JIA XuDong, LI Ning

文章导航 > Biomedical and Environmental Sciences > 2011 > 24(4): 415-421

ZHANG XiaoPeng, XIANG Qian, CUI WenMing, JIA XuDong, LI Ning. Evaluation of the in vitro and in vivo Genotoxicity of Almond Skins[J]. Biomedical and Environmental Sciences, 2011, 24(4): 415-421. doi: 10.3967/0895-3988.2011.04.013

Citation:

ZHANG XiaoPeng, XIANG Qian, CUI WenMing, JIA XuDong, LI Ning. Evaluation of the in vitro and in vivo Genotoxicity of Almond Skins[J]. Biomedical and Environmental Sciences, 2011, 24(4): 415-421. doi: 10.3967/0895-3988.2011.04.013

doi: 10.3967/0895-3988.2011.04.013

Evaluation of the in vitro and in vivo Genotoxicity of Almond Skins

Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China

摘要: Objective It aims to study potential genotoxicity of almond skins.Methods A bacterial reverse mutation assay was performed on S.typhimurium strains TA97,TA98,TA100,TA102,and TA1535 in the absence or presence of S-9 mixture at a dose range of 312.5 to 5 000 μg/plate.A micronucleus test and a mammalian bone marrow chromosome aberration tests were performed in Swiss Albino (CD-1) mice at doses of 625,1 250,and 2 500 mg/kg bw used.Results Almond skins exerted no mutagenic activity in various bacterial strains of Salmonella typhimurium in either the absence or the presence of metabolic activation at all doses tested.Various doses of almond skins did not affect the proportions of immature to total erythrocytes,the number of micronuclei in the immature erythrocytes,or the number of structural and numerical chromosomal aberrations of Swiss albino mice.Conclusion Almond skins are not genotoxic under the conditions of the in vitro bacterial reverse mutation assay and two in vivo tests - micronucleus test and mammalian bone marrow chromosome aberration test,which supports the safety of almond skins for dietary consumption.
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Abstract: Objective It aims to study potential genotoxicity of almond skins.Methods A bacterial reverse mutation assay was performed on S.typhimurium strains TA97,TA98,TA100,TA102,and TA1535 in the absence or presence of S-9 mixture at a dose range of 312.5 to 5 000 μg/plate.A micronucleus test and a mammalian bone marrow chromosome aberration tests were performed in Swiss Albino (CD-1) mice at doses of 625,1 250,and 2 500 mg/kg bw used.Results Almond skins exerted no mutagenic activity in various bacterial strains of Salmonella typhimurium in either the absence or the presence of metabolic activation at all doses tested.Various doses of almond skins did not affect the proportions of immature to total erythrocytes,the number of micronuclei in the immature erythrocytes,or the number of structural and numerical chromosomal aberrations of Swiss albino mice.Conclusion Almond skins are not genotoxic under the conditions of the in vitro bacterial reverse mutation assay and two in vivo tests - micronucleus test and mammalian bone marrow chromosome aberration test,which supports the safety of almond skins for dietary consumption.
- Almond skins /
- Genotoxicity /
- Mutagenicity /
- Micronuclei /
- Chromosome aberration

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Evaluation of the in vitro and in vivo Genotoxicity of Almond Skins

doi: 10.3967/0895-3988.2011.04.013

刊出日期: 2011-08-20

关键词:

Almond skins /

Genotoxicity /

Mutagenicity /

Micronuclei /

Chromosome aberration

摘要: Objective It aims to study potential genotoxicity of almond skins.Methods A bacterial reverse mutation assay was performed on S.typhimurium strains TA97,TA98,TA100,TA102,and TA1535 in the absence or presence of S-9 mixture at a dose range of 312.5 to 5 000 μg/plate.A micronucleus test and a mammalian bone marrow chromosome aberration tests were performed in Swiss Albino (CD-1) mice at doses of 625,1 250,and 2 500 mg/kg bw used.Results Almond skins exerted no mutagenic activity in various bacterial strains of Salmonella typhimurium in either the absence or the presence of metabolic activation at all doses tested.Various doses of almond skins did not affect the proportions of immature to total erythrocytes,the number of micronuclei in the immature erythrocytes,or the number of structural and numerical chromosomal aberrations of Swiss albino mice.Conclusion Almond skins are not genotoxic under the conditions of the in vitro bacterial reverse mutation assay and two in vivo tests - micronucleus test and mammalian bone marrow chromosome aberration test,which supports the safety of almond skins for dietary consumption.