Pravin Suryakantrao Deshmukh; Kanu Megha; Namita Nasare; Basu Dev Banerjee; Rafat Sultana Ahmed; Mahesh Pandurang Abegaonkar; Ashok Kumar Tripathi; Pramod Kumari Mediratta

doi:10.3967/bes2016.115

Effect of Low Level Subchronic Microwave Radiation on Rat Brain

Pravin Suryakantrao Deshmukh, Kanu Megha, Namita Nasare, Basu Dev Banerjee, Rafat Sultana Ahmed, Mahesh Pandurang Abegaonkar, Ashok Kumar Tripathi, Pramod Kumari Mediratta

文章导航 > Biomedical and Environmental Sciences > 2016 > 29(12): 858-867

Pravin Suryakantrao Deshmukh, Kanu Megha, Namita Nasare, Basu Dev Banerjee, Rafat Sultana Ahmed, Mahesh Pandurang Abegaonkar, Ashok Kumar Tripathi, Pramod Kumari Mediratta. Effect of Low Level Subchronic Microwave Radiation on Rat Brain[J]. Biomedical and Environmental Sciences, 2016, 29(12): 858-867. doi: 10.3967/bes2016.115

Citation:

Pravin Suryakantrao Deshmukh, Kanu Megha, Namita Nasare, Basu Dev Banerjee, Rafat Sultana Ahmed, Mahesh Pandurang Abegaonkar, Ashok Kumar Tripathi, Pramod Kumari Mediratta. Effect of Low Level Subchronic Microwave Radiation on Rat Brain[J]. Biomedical and Environmental Sciences, 2016, 29(12): 858-867. doi: 10.3967/bes2016.115

doi: 10.3967/bes2016.115

Effect of Low Level Subchronic Microwave Radiation on Rat Brain

Environmental Biochemistry and Molecular Biology Laboratory, Department of Biochemistry. University College of Medical Sciences & G.T.B. Hospital University of Delhi, Dilshad Garden, Delhi 110095, India
Centre for Applied Research in Electronics CARE, Indian Institute of Technology, Hauz Khas, New Delhi 110016, India
Department of Pharmacology, University College of Medical Sciences & G.T.B. Hospital University of Delhi, Dilshad Garden, Delhi 110095, India

摘要: Objective The present study was designed to investigate the effects of subchronic low level microwave radiation (MWR) on cognitive function, heat shock protein 70 (HSP70) level and DNA damage in brain of Fischer rats. Methods Experiments were performed on male Fischer rats exposed to microwave radiation for 90 days at three different frequencies: 900, 1800, and 2450 MHz. Animals were divided into 4 groups:Group I: Sham exposed, Group II: animals exposed to microwave radiation at 900 MHz and specific absorption rate (SAR) 5.953 × 10-4 W/kg, Group III: animals exposed to 1800 MHz at SAR 5.835 × 10-4 W/kg and Group IV: animals exposed to 2450 MHz at SAR 6.672 × 10-4 W/kg. All the animals were tested for cognitive function using elevated plus maze and Morris water maze at the end of the exposure period and subsequently sacrificed to collect brain tissues. HSP70 levels were estimated by ELISA and DNA damage was assessed using alkaline comet assay. Results Microwave exposure at 900-2450 MHz with SAR values as mentioned above lead to decline in cognitive function, increase in HSP70 level and DNA damage in brain. Conclusion The results of the present study suggest that low level microwave exposure at frequencies 900, 1800, and 2450 MHz may lead to hazardous effects on brain.
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Abstract: Objective The present study was designed to investigate the effects of subchronic low level microwave radiation (MWR) on cognitive function, heat shock protein 70 (HSP70) level and DNA damage in brain of Fischer rats. Methods Experiments were performed on male Fischer rats exposed to microwave radiation for 90 days at three different frequencies: 900, 1800, and 2450 MHz. Animals were divided into 4 groups:Group I: Sham exposed, Group II: animals exposed to microwave radiation at 900 MHz and specific absorption rate (SAR) 5.953 × 10-4 W/kg, Group III: animals exposed to 1800 MHz at SAR 5.835 × 10-4 W/kg and Group IV: animals exposed to 2450 MHz at SAR 6.672 × 10-4 W/kg. All the animals were tested for cognitive function using elevated plus maze and Morris water maze at the end of the exposure period and subsequently sacrificed to collect brain tissues. HSP70 levels were estimated by ELISA and DNA damage was assessed using alkaline comet assay. Results Microwave exposure at 900-2450 MHz with SAR values as mentioned above lead to decline in cognitive function, increase in HSP70 level and DNA damage in brain. Conclusion The results of the present study suggest that low level microwave exposure at frequencies 900, 1800, and 2450 MHz may lead to hazardous effects on brain.
- Brain /
- Cognitive function /
- Comet assay /
- DNA damage /
- HSP70 /
- Microwave radiation

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Effect of Low Level Subchronic Microwave Radiation on Rat Brain

doi: 10.3967/bes2016.115

刊出日期: 2016-12-20

关键词:

Brain /

Cognitive function /

Comet assay /

DNA damage /

HSP70 /

Microwave radiation

摘要: Objective The present study was designed to investigate the effects of subchronic low level microwave radiation (MWR) on cognitive function, heat shock protein 70 (HSP70) level and DNA damage in brain of Fischer rats. Methods Experiments were performed on male Fischer rats exposed to microwave radiation for 90 days at three different frequencies: 900, 1800, and 2450 MHz. Animals were divided into 4 groups:Group I: Sham exposed, Group II: animals exposed to microwave radiation at 900 MHz and specific absorption rate (SAR) 5.953 × 10-4 W/kg, Group III: animals exposed to 1800 MHz at SAR 5.835 × 10-4 W/kg and Group IV: animals exposed to 2450 MHz at SAR 6.672 × 10-4 W/kg. All the animals were tested for cognitive function using elevated plus maze and Morris water maze at the end of the exposure period and subsequently sacrificed to collect brain tissues. HSP70 levels were estimated by ELISA and DNA damage was assessed using alkaline comet assay. Results Microwave exposure at 900-2450 MHz with SAR values as mentioned above lead to decline in cognitive function, increase in HSP70 level and DNA damage in brain. Conclusion The results of the present study suggest that low level microwave exposure at frequencies 900, 1800, and 2450 MHz may lead to hazardous effects on brain.

English Abstract

Effect of Low Level Subchronic Microwave Radiation on Rat Brain

Environmental Biochemistry and Molecular Biology Laboratory, Department of Biochemistry. University College of Medical Sciences & G.T.B. Hospital University of Delhi, Dilshad Garden, Delhi 110095, India

Centre for Applied Research in Electronics CARE, Indian Institute of Technology, Hauz Khas, New Delhi 110016, India

Department of Pharmacology, University College of Medical Sciences & G.T.B. Hospital University of Delhi, Dilshad Garden, Delhi 110095, India

Publish Date: 2016-12-20

Keywords:

Abstract: Objective The present study was designed to investigate the effects of subchronic low level microwave radiation (MWR) on cognitive function, heat shock protein 70 (HSP70) level and DNA damage in brain of Fischer rats. Methods Experiments were performed on male Fischer rats exposed to microwave radiation for 90 days at three different frequencies: 900, 1800, and 2450 MHz. Animals were divided into 4 groups:Group I: Sham exposed, Group II: animals exposed to microwave radiation at 900 MHz and specific absorption rate (SAR) 5.953 × 10-4 W/kg, Group III: animals exposed to 1800 MHz at SAR 5.835 × 10-4 W/kg and Group IV: animals exposed to 2450 MHz at SAR 6.672 × 10-4 W/kg. All the animals were tested for cognitive function using elevated plus maze and Morris water maze at the end of the exposure period and subsequently sacrificed to collect brain tissues. HSP70 levels were estimated by ELISA and DNA damage was assessed using alkaline comet assay. Results Microwave exposure at 900-2450 MHz with SAR values as mentioned above lead to decline in cognitive function, increase in HSP70 level and DNA damage in brain. Conclusion The results of the present study suggest that low level microwave exposure at frequencies 900, 1800, and 2450 MHz may lead to hazardous effects on brain.

Citation:

Pravin Suryakantrao Deshmukh, Kanu Megha, Namita Nasare, Basu Dev Banerjee, Rafat Sultana Ahmed, Mahesh Pandurang Abegaonkar, Ashok Kumar Tripathi, Pramod Kumari Mediratta. Effect of Low Level Subchronic Microwave Radiation on Rat Brain[J]. Biomedical and Environmental Sciences, 2016, 29(12): 858-867. doi: 10.3967/bes2016.115