2016 Vol. 29, No. 5

Select articles
Development of a Novel PmpD-N ELISA for Chlamydia psittaci Infection
LIU Shan Shan, CHU Jun, ZHANG Qiang, SUN Wei, ZHANG Tian Yuan, HE Cheng
2016, 29(5): 315-322. doi: 10.3967/bes2016.041
ObjectiveChlamydia psittaci is an avian respiratory pathogen and zoonotic agent.The wide prevalence ofC. psittaci poses a threat to the poultry industry and its employees. However, few commercial kits are available for detecting avian antibodies excluding the in-house ELISA kit. In this study, we developed a novel ELISA kit for detecting antibodies againstC. psittaci based on the N-terminal fragment of polymorphic outer membrane protein D (PmpD-N) as the coating antigen.
Methods The antigen concentrations, primary antibody, and cut-off value were determined and optimized. The ELISA, designated PmpD-N ELISA, was assessed for sensitivity, specificity, and concordance using sera samples from 48 experimentally infected and 168 uninfected SPF chickens.
Results The sensitivity and specificity of PmpD-N ELISA were 97.9%, 100%, respectively, while the concordance was 98.1% as compared to that of MOMP-ELISA. No cross-reaction with positive sera for other avian pathogens was found. Using PmpD-N ELISA, 799/836 clinical samples were positive, including 93.0% and 98.1% positivity in layers and broilers, respectively.
Conclusion These data indicate that indirect ELISA with PmpD-N as the antigen candidate is a promising approach for the surveillance ofC. psittaci infection.
Evaluation of Six Recombinant Proteins for Serological Diagnosis of Lyme Borreliosis in China
LIU Wei, LIU Hui Xin, ZHANG Lin, HOU Xue Xia, WAN Kang Lin, HAO Qin
2016, 29(5): 323-330. doi: 10.3967/bes2016.042
ObjectiveIn this study, we evaluated the diagnostic efficiency of six recombinant proteins for the serodiagnosis of Lyme borreliosis (LB) and screened out the appropriate antigens to support the production of a Chinese clinical ELISA (enzyme-linked immunosorbent assay) kit for LB.
MethodsSix recombinant antigens, Fla B.g, OspC B.a, OspC B.g, P39 B.g, P83 B.g, and VlsE B.a, were used for ELISA to detect serum antibodies in LB, syphilis, and healthy controls. The ELISA results were used to generate receiver operating characteristic (ROC) curves, and the sensitivity and specificity of each protein was evaluated. All recombinant proteins were evaluated and screened by using logistic regression models.
ResultsTwo IgG (VlsE and OspC B.g) and two IgM (OspC B.g and OspC B.a) antigens were left by the logistic regression model screened. VlsE had the highest specificity for syphilis samples in the IgG test (87.7%,P<0.05). OspC B.g had the highest diagnostic value in the IgM test (AUC=0.871). Interactive effects between OspC B.a and Fla B.g could reduce the specificity of the ELISA.
ConclusionThree recombinant antigens, OspC B.g, OspC B.a, and VlsE B.a, were useful for ELISAs of LB. Additionally, the interaction between OspC B.a and Fla B.g should be examined in future research.
Viral Etiology Relationship between Human Papillomavirus and Human Breast Cancer and Target of Gene Therapy
CHEN Yun Xin, SHEN Dan Hua, LI Jin Tao
2016, 29(5): 331-339. doi: 10.3967/bes2016.043
ObjectiveTo explore the viral etiology of human breast cancer to determine whether there are novel molecular targets for gene therapy of breast cancer and provide evidence for the research of gene therapy and vaccine development for breast cancer.
MethodsPCR was used to screen HPV16 and HPV18 oncogenesE6 andE7 in the SKBR3 cell line andin 76 paraffin embedded breast cancer tissue samples. RNA interference was used to knock down the expression of HPV18E6 andE7 in SKBR3 cells, then the changes in the expression of cell-cycle related proteins, cell viability, colony formation, metastasis, and cell cycle progression were determined.
ResultsHPV18 oncogenesE6 andE7 were amplified and sequenced from the SKBR3 cells. Ofthe patient samples, 6.58% and 23.68% were tested to bepositivefor HPV18E6 and HPV18E7. In the cell culture models, the knockdown of HPV18E6 andE7 inhibited the proliferation, metastasis, and cell cycle progression of SKBR3 cell. The knockdown also clearly affected the expression levels of cell cycle related proteins.
ConclusionHPV was a contributor to virus causedhuman breast cancer, suggesting that the oncogenes in HPV were potential targets for gene therapy of breast cancer.
Whole Genome Sequencing and Comparisons of Different Chinese Rabies Virus Lineages Including the First Complete Genome of an Arctic-like Strain in China
LI Hao, GUO ZhenYang, ZHANG Jian, TAO XiaoYan, ZHU WuYang, TANG Qing, LIU HongTu
2016, 29(5): 340-346. doi: 10.3967/bes2016.044
ObjectiveTo learn the rabies genome molecular characteristics and compare the difference of China rabies lineages.
MethodsThe complete genomes of 12 strains from different China rabies lineages were amplified and sequenced, and all the China street strain genomes (total 43), Arctic and Arctic-like genomes were aligned using ClustalX2, the genome homologies were analyzed using MegAlign software, and the phylogenetic trees were constructed by MEGA 5.
ResultsFirst Arctic-like rabies genome in China (CQH1202D) was reported, and we supplemented the rabies genome data of China, ensuring at least one genome was available in each China lineage. The genome size of China V (11908nt) is obviously shorter than other lineages’ (11923-11925nt) for the difference of N-P non-coding regions. Among different lineages, the genome homologies are almost under 90%.CQH1202D (China IV lineage) has close relationship with strains from South Korea and they share about 95% genome similarities.
ConclusionThe molecular characteristics of 6 different China rabies lineages werecompared and analyzed from genome level, which benefits for continued comprehensive rabies surveillance, rabies prevention and control in China.
The Status and Associated Factors of Successful Aging among Older Adults Residing in Longevity Areas in China
SHI Wen Hui, ZHANG Hong Yan, ZHANG Juan, LYU Yue Bin, Melanie Sereny Brasher, YIN Zhao Xue, LUO Jie Si, HU Dong Sheng, FEN Lei, andSHI Xiao Ming
2016, 29(5): 347-355. doi: 10.3967/bes2016.045
ObjectiveThis study aims to assess the status of successful aging (SA) in longevity areas in Chinaand explore multiple factors associated with SA among the young-old and oldest-old.
MethodsA total of 2296 elderly people aged 65 and older were interviewed in the longevity areas sub-sample of the Chinese Longitudinal Healthy Longevity Survey (CLHLS) in 2012. Baseline assessments included a researcher-administered questionnaire, physical examination, and laboratory testing. A logistic regression model was used to identify factors associated with SA.
ResultsThe prevalenceof SA was 38.81% in the CLHLS in 2012. There were significant differences between ages groups, with SA compromising 56.85%among≥65 years group and 20.31% among ≥100 years group (χ2trend=126.73,P<0.01). The prevalence of SA among females was 33.59%, which was significantly lower than that among males (45.58%) (χ2gender=33.65,P<0.05). In the regression analysis, having anemia (OR=0.744, 95% CI: 0.609-0.910), poor lifestyle (OR=0.697, 95% CI: 0.568-0.854), poor sleep quality (OR=0.558, 95% CI: 0.456-0.682), and central obesity (OR=0.684, 95% CI: 0.556-0.841) were the main factors associated with SA. The promoting SA rate decreased as age increased, and the group of 65-79 years had higher odds than the other age group.
ConclusionPreventing central obesity, improving sleep quality and promoting healthy lifestyle may contribute to achieve SA among the elderly.
Cognitive Training in Older Adults with Mild Cognitive Impairment
LIU Xin Yan, LI Li, XIAO Jia Qing, HE Chang Zhi, LYU Xiu Lin, GAO Lei, YANG Xiao Wei, CUI Xin Gang, FAN Li Hua
2016, 29(5): 356-364. doi: 10.3967/bes2016.046
ObjectiveWe investigated the feasibility and efficacy of cognitive training for older adults in rural settings and with low education levels, who have mild cognitive impairment (MCI).
MethodsForty-five older adults (ages >65 years) with MCI were assigned to treatment or control groups, at a 2:1 ratio. Cognitivetraining occurred in the treatment group for 2 months. The cognitive abilities of the participants were assessed at pre-training, metaphase, and post-training time points, using the Mini-Mental State Examination (MMSE), Montreal Cognitive Assessment (MoCA), Loewenstein Occupational Therapy Cognitive Assessment (LOTCA), and Hamilton Depression Scale (HAM-D).
ResultsFollowing training, cognitive abilities improved in the treatment group, based on the total scores of all 4 measures, as well as specifically on the MoCA and LOTCA. There were differences in the main effects of group and time point on some subscales, but these differences had little, if any, effect on the overall analyses.
ConclusionThe present study demonstrated that cognitive training has beneficial effects on attention, language, orientation, visual perception, organization of visual movement, and logical questioning in patients with MCI. Furthermore, the observed effects are long-term changes.
Dietary Exposure to Benzyl Butyl Phthalate in China
ZHANG Lei, JIANG Ding Guo, SUI Hai Xia, WU Ping Gu, LIU Ai Dong, YANG Da Jin, LIU Zhao Ping, SONG Yan, LI Ning
2016, 29(5): 365-373. doi: 10.3967/bes2016.047
ObjectiveBenzyl butyl phthalate (BBP) is a plasticizer used in food contact materials. Dietary exposure to BBP might lead to reproduction and developmental damages to human. The present paper was aimed to assess the health risk of BBP dietary exposure in Chinese population.
MethodsThe BBP contents were detected in 7409 food samples from 25 foodcategories by gas chromatography-mass spectrometry operated in selected ion monitoring (SIM) mode. The dietary exposures of BBP in different age and sex groups were estimated by combining the content data with food consumption data derived from 2002 China National Nutrient and Health Survey, and evaluated according to the tolerable daily intake (TDI) of BBP established by European Food safety Agency.
ResultsIt was found that BBP was undetectable in most samples and the highest level was 1.69 mg/kg detected in a vegetable oil sample. The average dietary exposure of BBP in people aged≥2 years was
1.03 μg/kgbw perday and the highest average exposure was found in 2-6 years old children (1.98 μg/kg bw perday). The BBP exposure in 7-12 months old children excessed 10% of tolerable daily intake (TDI) in worst scenario.
ConclusionThe health risk of BBP dietary exposure in Chinese population is low and, considering BBP alone, there is no safety concern.
Rapid and Sensitive Chemiluminescent Enzyme Immunoassay for the Determination of NeomycinResidues in Milk
LUO Peng Jie, ZHANG Jian Bo, WANG Hua Li, WU Nan, ZHAO Yun Feng, WANGXiaoMei, ZHANGHong, ZHANGJiYue, ZHULei, JIANGWenXiao
2016, 29(5): 374-378. doi: 10.3967/bes2016.048
Occupational Exposure to Indium of Indium Smelter Workers
DING Chun Guang, WANG Huan Qiang, SONG Han Bo, LI Zhi Hui, LI Xiao Ping, YE Shao Se, ZHANG Fu Gang, CUI Shi Wei, YAN Hui Fang, LI Tao
2016, 29(5): 379-384. doi: 10.3967/bes2016.049
Circulating Micro RNA-21is Downregulatedin Patients with Metabolic Syndrome
HE Qing Fang, WANG Li Xin, ZHONG Jie Ming, HU Ru Ying, FANG Le, WANG Hao, GONG Wei Wei, ZHANG Jie, PAN Jing, YU Min#
2016, 29(5): 385-389. doi: 10.3967/bes2016.050