Evaluation of a New Real-time PCR Assay for Detection of Mycoplasma Pneumoniae in Clinical Specimens

ZHAO Fei; CAO Bin; HE Li Hua; YIN Yu Dong; TAO Xiao Xia; SONG Shu Fan; MENG Fan Liang; ZHANG Jian Zhong

doi:10.3967/0895-3988.2012.01.011

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Volume 25 Issue 1

Feb. 2012

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Article Navigation > Biomedical and Environmental Sciences > 2012 > 25(1): 77-81

ZHAO Fei, CAO Bin, HE Li Hua, YIN Yu Dong, TAO Xiao Xia, SONG Shu Fan, MENG Fan Liang, ZHANG Jian Zhong. Evaluation of a New Real-time PCR Assay for Detection of Mycoplasma Pneumoniae in Clinical Specimens[J]. Biomedical and Environmental Sciences, 2012, 25(1): 77-81. doi: 10.3967/0895-3988.2012.01.011

Citation:

ZHAO Fei, CAO Bin, HE Li Hua, YIN Yu Dong, TAO Xiao Xia, SONG Shu Fan, MENG Fan Liang, ZHANG Jian Zhong. Evaluation of a New Real-time PCR Assay for Detection of Mycoplasma Pneumoniae in Clinical Specimens[J]. Biomedical and Environmental Sciences, 2012, 25(1): 77-81. doi: 10.3967/0895-3988.2012.01.011

Evaluation of a New Real-time PCR Assay for Detection of Mycoplasma Pneumoniae in Clinical Specimens

doi: 10.3967/0895-3988.2012.01.011

National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;State Key Laboratory for Infectious Disease Prevention and Control Beijing 102206, China
Department of Infectious Diseases and Clinical Microbiology, Beijing Chao-Yang Hospital, Beijing Institute of Respiratory Medicine, Capital Medical University, Beijing 100020, China;Beijing Key Laboratory of Respiratory and Pulmonary Circulation, Beijing 100020, China

Funds: This study was supported by the National Key Program for Infectious Diseases of China(2008ZX10004-002)

Abstract

Objective To establish and evaluate a real-time PCR assay to detect Mycoplasma pneumoniae (M.pneumoniae) in clinical specimens.Methods By analysing the whole p1 gene sequence of 60 M.pneumoniae clinical isolates in Beijing of China,an optimized real-time PCR assay (MpP1) using p1 gene conserved region was designed.The specificity and sensitivity of this assay were evaluated and compared with other two reported assays (RepMp1 and Mp181) using 40 positive and 100 negative clinical specimens.Results The detection limit of the new assay was 8.1 fg (about 1～3CFU) M.pneumoniae DNA.The sensitivity of Mpp1,RepMp1,and Mp181 assays appeared to be 100％,100％,and 85％,respectively.Conclusion MpP1 assay is suitable for the detection of M.pneumoniae in Chinese clinical specimens.
- Mycoplasma pneumoniae,
- Real-time PCR,
- p1 gene
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Evaluation of a New Real-time PCR Assay for Detection of Mycoplasma Pneumoniae in Clinical Specimens

doi: 10.3967/0895-3988.2012.01.011

National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;State Key Laboratory for Infectious Disease Prevention and Control Beijing 102206, China

Department of Infectious Diseases and Clinical Microbiology, Beijing Chao-Yang Hospital, Beijing Institute of Respiratory Medicine, Capital Medical University, Beijing 100020, China;Beijing Key Laboratory of Respiratory and Pulmonary Circulation, Beijing 100020, China

Funds: This study was supported by the National Key Program for Infectious Diseases of China(2008ZX10004-002)

Key words:

Abstract: Objective To establish and evaluate a real-time PCR assay to detect Mycoplasma pneumoniae (M.pneumoniae) in clinical specimens.Methods By analysing the whole p1 gene sequence of 60 M.pneumoniae clinical isolates in Beijing of China,an optimized real-time PCR assay (MpP1) using p1 gene conserved region was designed.The specificity and sensitivity of this assay were evaluated and compared with other two reported assays (RepMp1 and Mp181) using 40 positive and 100 negative clinical specimens.Results The detection limit of the new assay was 8.1 fg (about 1～3CFU) M.pneumoniae DNA.The sensitivity of Mpp1,RepMp1,and Mp181 assays appeared to be 100％,100％,and 85％,respectively.Conclusion MpP1 assay is suitable for the detection of M.pneumoniae in Chinese clinical specimens.

Citation:

ZHAO Fei, CAO Bin, HE Li Hua, YIN Yu Dong, TAO Xiao Xia, SONG Shu Fan, MENG Fan Liang, ZHANG Jian Zhong. Evaluation of a New Real-time PCR Assay for Detection of Mycoplasma Pneumoniae in Clinical Specimens[J]. Biomedical and Environmental Sciences, 2012, 25(1): 77-81. doi: 10.3967/0895-3988.2012.01.011