Preparation and Initial Application of a Monoclonal Antibody Specific for a Newly Discovered Conserved Linear Epitope of Rabies Virus Nucleoprotein

LV Xin Jun; MA Xue Jun; WANG Li Hua; LI Hao; SHEN Xin Xin; YU Peng Cheng; TANG Qing; LIANG Guo Dong

doi:10.3967/0895-3988.2012.01.014

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Volume 25 Issue 1

Feb. 2012

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Article Navigation > Biomedical and Environmental Sciences > 2012 > 25(1): 98-103

LV Xin Jun, MA Xue Jun, WANG Li Hua, LI Hao, SHEN Xin Xin, YU Peng Cheng, TANG Qing, LIANG Guo Dong. Preparation and Initial Application of a Monoclonal Antibody Specific for a Newly Discovered Conserved Linear Epitope of Rabies Virus Nucleoprotein[J]. Biomedical and Environmental Sciences, 2012, 25(1): 98-103. doi: 10.3967/0895-3988.2012.01.014

Citation:

LV Xin Jun, MA Xue Jun, WANG Li Hua, LI Hao, SHEN Xin Xin, YU Peng Cheng, TANG Qing, LIANG Guo Dong. Preparation and Initial Application of a Monoclonal Antibody Specific for a Newly Discovered Conserved Linear Epitope of Rabies Virus Nucleoprotein[J]. Biomedical and Environmental Sciences, 2012, 25(1): 98-103. doi: 10.3967/0895-3988.2012.01.014

Preparation and Initial Application of a Monoclonal Antibody Specific for a Newly Discovered Conserved Linear Epitope of Rabies Virus Nucleoprotein

doi: 10.3967/0895-3988.2012.01.014

State Key Laboratory for Infectious Disease Prevention and Control Department of Viral Encephalitis, Institute for Viral Disease Control and Prevention, Chinese Centers for Disease Control and Prevention, Beijing 102206,China
State Key Laboratory for Molecular Virology and Genetic Engineering, Department of Core Facility,Institute for Viral Disease Control and Prevention, Chinese Centers for Disease Control and Prevention, Beijing 102206, China

Funds: This study was supported by research grants from the Diagnosis of Infectious Pathogens and Combination of Diagnostic Technologies(2008ZX10004-002)%Prevention and Control of Major Infectious Disease such as AIDS and Viral Hepatitis,State Eleventh Five-Year Plan

Abstract

Objective To prepare monoclonal antibodies against a newly discovered and conserved linear epitope of Rabies virus nucleoprotein and to use them in a rabies diagnostic test.Methods Synthetic peptide containing the epitope was used as immunogen to prepare hybridoma cell lines by classical hybridoma technology.Anti-peptide monoclonal antibodies produced in ascites of inoculated Balb/c mice were labeled with fluorescein isothiocyanate (FITC) after purification and used in fluorescent antibody test (FAT). Results Two positive hybridoma cell lines,RVNP-mAb1-CL and RVNP-mAb2-CL,were obtained.RVNPmAb1-CL produced a higher concentration of monoclonal antibody RVNP-mAb1 in Balb/c ascites.FITC-labeled RVNP-mAb1 showed correct results on certain Rabies virus-positive canine brain tissue samples and cells of a small subclone of baby hamster kidney 21 cell line (BSR).Conclusion FITC-labeled RVNP-mAb1 has potential application for laboratory diagnosis of rabies.
- Rabies virus,
- Nucleoprotein,
- Epitope,
- Peptide,
- Monoclonal antibody
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Preparation and Initial Application of a Monoclonal Antibody Specific for a Newly Discovered Conserved Linear Epitope of Rabies Virus Nucleoprotein

doi: 10.3967/0895-3988.2012.01.014

State Key Laboratory for Infectious Disease Prevention and Control Department of Viral Encephalitis, Institute for Viral Disease Control and Prevention, Chinese Centers for Disease Control and Prevention, Beijing 102206,China

State Key Laboratory for Molecular Virology and Genetic Engineering, Department of Core Facility,Institute for Viral Disease Control and Prevention, Chinese Centers for Disease Control and Prevention, Beijing 102206, China

Key words:

Abstract: Objective To prepare monoclonal antibodies against a newly discovered and conserved linear epitope of Rabies virus nucleoprotein and to use them in a rabies diagnostic test.Methods Synthetic peptide containing the epitope was used as immunogen to prepare hybridoma cell lines by classical hybridoma technology.Anti-peptide monoclonal antibodies produced in ascites of inoculated Balb/c mice were labeled with fluorescein isothiocyanate (FITC) after purification and used in fluorescent antibody test (FAT). Results Two positive hybridoma cell lines,RVNP-mAb1-CL and RVNP-mAb2-CL,were obtained.RVNPmAb1-CL produced a higher concentration of monoclonal antibody RVNP-mAb1 in Balb/c ascites.FITC-labeled RVNP-mAb1 showed correct results on certain Rabies virus-positive canine brain tissue samples and cells of a small subclone of baby hamster kidney 21 cell line (BSR).Conclusion FITC-labeled RVNP-mAb1 has potential application for laboratory diagnosis of rabies.

Citation:

LV Xin Jun, MA Xue Jun, WANG Li Hua, LI Hao, SHEN Xin Xin, YU Peng Cheng, TANG Qing, LIANG Guo Dong. Preparation and Initial Application of a Monoclonal Antibody Specific for a Newly Discovered Conserved Linear Epitope of Rabies Virus Nucleoprotein[J]. Biomedical and Environmental Sciences, 2012, 25(1): 98-103. doi: 10.3967/0895-3988.2012.01.014