Preparation and Initial Application of a Monoclonal Antibody Specific for a Newly Discovered Conserved Linear Epitope of Rabies Virus Nucleoprotein
Abstract: Objective To prepare monoclonal antibodies against a newly discovered and conserved linear epitope of Rabies virus nucleoprotein and to use them in a rabies diagnostic test.Methods Synthetic peptide containing the epitope was used as immunogen to prepare hybridoma cell lines by classical hybridoma technology.Anti-peptide monoclonal antibodies produced in ascites of inoculated Balb/c mice were labeled with fluorescein isothiocyanate (FITC) after purification and used in fluorescent antibody test (FAT). Results Two positive hybridoma cell lines,RVNP-mAb1-CL and RVNP-mAb2-CL,were obtained.RVNPmAb1-CL produced a higher concentration of monoclonal antibody RVNP-mAb1 in Balb/c ascites.FITC-labeled RVNP-mAb1 showed correct results on certain Rabies virus-positive canine brain tissue samples and cells of a small subclone of baby hamster kidney 21 cell line (BSR).Conclusion FITC-labeled RVNP-mAb1 has potential application for laboratory diagnosis of rabies.
|LV Xin Jun, MA Xue Jun, WANG Li Hua, LI Hao, SHEN Xin Xin, YU Peng Cheng, TANG Qing, LIANG Guo Dong. Preparation and Initial Application of a Monoclonal Antibody Specific for a Newly Discovered Conserved Linear Epitope of Rabies Virus Nucleoprotein[J]. Biomedical and Environmental Sciences, 2012, 25(1): 98-103. doi: 10.3967/0895-3988.2012.01.014